ID   MB3L2_HUMAN             Reviewed;         208 AA.
AC   Q8NHZ7;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 3.
DT   24-JAN-2024, entry version 145.
DE   RecName: Full=Methyl-CpG-binding domain protein 3-like 2;
DE            Short=MBD3-like protein 2;
GN   Name=MBD3L2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=12504854; DOI=10.1006/geno.2002.7001;
RA   Jiang C.-L., Jin S.-G., Lee D.-H., Lan Z.-J., Xu X., O'Connor T.R.,
RA   Szabo P.E., Mann J.R., Cooney A.J., Pfeifer G.P.;
RT   "MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding
RT   proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific
RT   transcriptional repressor.";
RL   Genomics 80:621-629(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-208.
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, INTERACTION WITH MBD3; MTA1; HDAC1; HDAC2; RBBP4 AND RBBP7,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=15701600; DOI=10.1074/jbc.m413492200;
RA   Jin S.-G., Jiang C.-L., Rauch T., Li H., Pfeifer G.P.;
RT   "MBD3L2 interacts with MBD3 and components of the NuRD complex and can
RT   oppose MBD2-MeCP1-mediated methylation silencing.";
RL   J. Biol. Chem. 280:12700-12709(2005).
CC   -!- FUNCTION: May displace the NuRD complex from chromatin.
CC       {ECO:0000269|PubMed:15701600}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with MBD3; the interaction is
CC       direct (PubMed:15701600). Interacts with MTA1 (PubMed:15701600).
CC       Interacts with HDAC1 (PubMed:15701600). Interacts with HDAC2
CC       (PubMed:15701600). Interacts with RBBP4 (PubMed:15701600). Interacts
CC       with RBBP7 (PubMed:15701600). {ECO:0000269|PubMed:15701600}.
CC   -!- INTERACTION:
CC       Q8NHZ7; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-11989378, EBI-5916454;
CC       Q8NHZ7; Q96D09: GPRASP2; NbExp=3; IntAct=EBI-11989378, EBI-473189;
CC       Q8NHZ7; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-11989378, EBI-3044087;
CC       Q8NHZ7; O76011: KRT34; NbExp=3; IntAct=EBI-11989378, EBI-1047093;
CC       Q8NHZ7; Q99750: MDFI; NbExp=3; IntAct=EBI-11989378, EBI-724076;
CC       Q8NHZ7; Q59EK9-3: RUNDC3A; NbExp=3; IntAct=EBI-11989378, EBI-11957366;
CC       Q8NHZ7; P0C264: SBK3; NbExp=3; IntAct=EBI-11989378, EBI-17181801;
CC       Q8NHZ7; Q8N7C3: TRIML2; NbExp=3; IntAct=EBI-11989378, EBI-11059915;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15701600}.
CC   -!- TISSUE SPECIFICITY: Detected at low levels in several somatic tissues
CC       (PubMed:12504854, PubMed:15701600). Highly expressed in the ovarian
CC       teratocarcinoma cell line PA-1 (PubMed:12504854).
CC       {ECO:0000269|PubMed:12504854, ECO:0000269|PubMed:15701600}.
CC   -!- MISCELLANEOUS: The MBD3L proteins are encoded by strongly repeated
CC       regions of the 19p13 chromosome. The exact number of functional copies
CC       is unclear, and some of them may represent pseudogenes.
CC   -!- SIMILARITY: Belongs to the MBD3L family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM28154.2; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF503919; AAM28154.2; ALT_FRAME; mRNA.
DR   EMBL; AC010606; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BE502398; -; NOT_ANNOTATED_CDS; mRNA.
DR   CCDS; CCDS42483.1; -.
DR   RefSeq; NP_653215.2; NM_144614.3.
DR   AlphaFoldDB; Q8NHZ7; -.
DR   SMR; Q8NHZ7; -.
DR   BioGRID; 125944; 54.
DR   IntAct; Q8NHZ7; 8.
DR   STRING; 9606.ENSP00000370800; -.
DR   BioMuta; MBD3L2; -.
DR   MassIVE; Q8NHZ7; -.
DR   PaxDb; 9606-ENSP00000370800; -.
DR   PeptideAtlas; Q8NHZ7; -.
DR   Antibodypedia; 68491; 81 antibodies from 11 providers.
DR   DNASU; 125997; -.
DR   Ensembl; ENST00000381393.3; ENSP00000370800.2; ENSG00000230522.5.
DR   GeneID; 125997; -.
DR   KEGG; hsa:125997; -.
DR   MANE-Select; ENST00000381393.3; ENSP00000370800.2; NM_144614.4; NP_653215.2.
DR   UCSC; uc010dvf.2; human.
DR   AGR; HGNC:18532; -.
DR   CTD; 125997; -.
DR   DisGeNET; 125997; -.
DR   GeneCards; MBD3L2; -.
DR   HGNC; HGNC:18532; MBD3L2.
DR   HPA; ENSG00000230522; Not detected.
DR   MIM; 607964; gene.
DR   neXtProt; NX_Q8NHZ7; -.
DR   OpenTargets; ENSG00000230522; -.
DR   PharmGKB; PA38567; -.
DR   VEuPathDB; HostDB:ENSG00000230522; -.
DR   eggNOG; KOG4161; Eukaryota.
DR   GeneTree; ENSGT00950000183005; -.
DR   InParanoid; Q8NHZ7; -.
DR   OMA; YNERERT; -.
DR   OrthoDB; 4849303at2759; -.
DR   PhylomeDB; Q8NHZ7; -.
DR   TreeFam; TF325032; -.
DR   PathwayCommons; Q8NHZ7; -.
DR   SignaLink; Q8NHZ7; -.
DR   BioGRID-ORCS; 125997; 56 hits in 673 CRISPR screens.
DR   GenomeRNAi; 125997; -.
DR   Pharos; Q8NHZ7; Tdark.
DR   PRO; PR:Q8NHZ7; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q8NHZ7; Protein.
DR   Bgee; ENSG00000230522; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 3 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0008327; F:methyl-CpG binding; IBA:GO_Central.
DR   GO; GO:0006346; P:DNA methylation-dependent heterochromatin formation; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:1902275; P:regulation of chromatin organization; IDA:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   InterPro; IPR032343; MBD2/MBD3_p55-bd.
DR   InterPro; IPR025884; MeCpG-bd_2/3_C_dom.
DR   Pfam; PF14048; MBD_C; 1.
DR   Pfam; PF16564; MBDa; 1.
DR   Genevisible; Q8NHZ7; HS.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..208
FT                   /note="Methyl-CpG-binding domain protein 3-like 2"
FT                   /id="PRO_0000096253"
FT   REGION          1..89
FT                   /note="Interacts with MBD3"
FT                   /evidence="ECO:0000269|PubMed:15701600"
FT   CONFLICT        124
FT                   /note="G -> S (in Ref. 1; AAM28154 and 3; BE502398)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        207..208
FT                   /note="GG -> CR (in Ref. 1; AAM28154)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   208 AA;  22994 MW;  55F7F5E2275F15CB CRC64;
     MGEPAFTSFP SLPVLGKLKR NMMPWALQKK REIHMAKAHR RRAARSALPM RLTSCIFRRP
     VTRIRSHPDN QVRRRKGDEH LEKPQQLCAY RRLQALQPCS SQGEGSSPLH LESVLSILAP
     GTAGESLDRA GAERVRSPLE PTPGRFPAVA GGPTPGMGCQ LPPPLSGQLV TPADIRRQAR
     RVKKARERLA KALQADRLAR RAEMLTGG
//