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Protein

Transcription factor MafA

Gene

MAFA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.6 Publications

GO - Molecular functioni

GO - Biological processi

  • insulin secretion Source: BHF-UCL
  • nitric oxide mediated signal transduction Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB
  • response to glucose Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-210745 Regulation of gene expression in beta cells
SIGNORiQ8NHW3

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafA
Alternative name(s):
Pancreatic beta-cell-specific transcriptional activator
Transcription factor RIPE3b1
V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
Gene namesi
Name:MAFA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000182759.3
HGNCiHGNC:23145 MAFA
MIMi610303 gene
neXtProtiNX_Q8NHW3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14S → A: Abolishes transactivation activity; when associated with A-65. 1 Publication1
Mutagenesisi49S → A: Diminishes transcriptional activity and transforming activity and abolishes ubiquitination; when associated with A-53; A-57 and A-61. 1 Publication1
Mutagenesisi53T → A: Diminishes transcriptional activity and transforming activity and abolishes ubiquitination; when associated with A-49; A-57 and A-61. 1 Publication1
Mutagenesisi57T → A: Diminishes transcriptional activity and transforming activity and abolishes ubiquitination; when associated with A-49; A-53 and A-61. 1 Publication1
Mutagenesisi61S → A: Diminishes transcriptional activity and transforming activity and abolishes ubiquitination; when associated with A-49; A-53 and A-57. 1 Publication1
Mutagenesisi65S → A: Greatly reduces phosphorylation and reduces transcriptional activity; when associated with A-14. 1 Publication1

Keywords - Diseasei

Proto-oncogene, Tumor suppressor

Organism-specific databases

DisGeNETi389692
OpenTargetsiENSG00000182759
PharmGKBiPA134963361

Polymorphism and mutation databases

BioMutaiMAFA
DMDMi296435511

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003202741 – 353Transcription factor MafAAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14Phosphoserine; by MAPK11 Publication1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei49Phosphoserine; by GSK31 Publication1
Modified residuei53Phosphothreonine; by GSK31 Publication1
Modified residuei57Phosphothreonine; by GSK31 Publication1
Modified residuei61Phosphoserine; by GSK31 Publication1
Modified residuei65Phosphoserine; by MAPK11 Publication1

Post-translational modificationi

Ubiquitinated, leading to its degradation by the proteasome.1 Publication
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through a ubiquitin/proteasome-dependent pathway.Curated3 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8NHW3
PeptideAtlasiQ8NHW3
PRIDEiQ8NHW3
TopDownProteomicsiQ8NHW3

PTM databases

iPTMnetiQ8NHW3
PhosphoSitePlusiQ8NHW3

Expressioni

Inductioni

Up-regulated by glucose.1 Publication

Gene expression databases

BgeeiENSG00000182759
CleanExiHS_MAFA

Interactioni

Subunit structurei

Binds DNA as a homodimer. Interacts with NEUROD1, PCAF and PDX1.3 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi133233, 9 interactors
STRINGi9606.ENSP00000328364

Structurei

Secondary structure

1353
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi229 – 234Combined sources6
Helixi237 – 243Combined sources7
Turni244 – 246Combined sources3
Helixi249 – 316Combined sources68

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EOTX-ray2.86A/B226-318[»]
ProteinModelPortaliQ8NHW3
SMRiQ8NHW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini254 – 317bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 279Basic motifAdd BLAST26
Regioni260 – 274Interaction with DNAAdd BLAST15
Regioni282 – 303Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 86Poly-GlyAdd BLAST13
Compositional biasi147 – 220His-richAdd BLAST74

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISHR Eukaryota
ENOG4112BJ1 LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000261683
HOVERGENiHBG000313
InParanoidiQ8NHW3
KOiK07595
OMAiQRHILES
OrthoDBiEOG091G0H46
PhylomeDBiQ8NHW3
TreeFamiTF325689

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR013592 Maf_TF_N
IPR028562 MafA
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF30 PTHR10129:SF30, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
PF08383 Maf_N, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NHW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL
60 70 80 90 100
SSTPLSTPCS SVPSSPSFCA PSPGTGGGGG AGGGGGSSQA GGAPGPPSGG
110 120 130 140 150
PGAVGGTSGK PALEDLYWMS GYQHHLNPEA LNLTPEDAVE ALIGSGHHGA
160 170 180 190 200
HHGAHHPAAA AAYEAFRGPG FAGGGGADDM GAGHHHGAHH AAHHHHAAHH
210 220 230 240 250
HHHHHHHHGG AGHGGGAGHH VRLEERFSDD QLVSMSVREL NRQLRGFSKE
260 270 280 290 300
EVIRLKQKRR TLKNRGYAQS CRFKRVQQRH ILESEKCQLQ SQVEQLKLEV
310 320 330 340 350
GRLAKERDLY KEKYEKLAGR GGPGSAGGAG FPREPSPPQA GPGGAKGTAD

FFL
Length:353
Mass (Da):36,982
Last modified:May 18, 2010 - v2
Checksum:i38F732D4C959AD62
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199Missing in BAC20389 (PubMed:12368292).Curated1
Sequence conflicti199Missing in AAL89527 (PubMed:12011435).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086960 Genomic DNA Translation: BAC20389.1
AY083269 Genomic DNA Translation: AAL89527.1
AC105118 Genomic DNA No translation available.
CCDSiCCDS34955.1
RefSeqiNP_963883.2, NM_201589.3
UniGeneiHs.521914

Genome annotation databases

EnsembliENST00000333480; ENSP00000328364; ENSG00000182759
GeneIDi389692
KEGGihsa:389692
UCSCiuc003yyc.3 human

Similar proteinsi

Entry informationi

Entry nameiMAFA_HUMAN
AccessioniPrimary (citable) accession number: Q8NHW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 18, 2010
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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