Q8NHV4 (NEDD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NEDD1

Alternative name(s):
Neural precursor cell expressed developmentally down-regulated protein 1
Short name=NEDD-1

Gene names

Name:

NEDD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. Ref.5 Ref.8
Subunit structure	Interacts with FAM29A and gamma-tubulin. Ref.5
Subcellular location	Cytoplasm › cytoskeleton › centrosome Ref.5.
Post-translational modification	During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Ref.8
Sequence similarities	Contains 8 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Traceable author statement. Source: Reactome cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from direct assay PubMed 21399614. Source: UniProtKB cytosol Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NHV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NHV4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Protein NEDD1		PRO_0000051095
Regions
	Repeat	1 – 31	31	WD 1
	Repeat	32 – 71	40	WD 2
	Repeat	75 – 114	40	WD 3
	Repeat	117 – 156	40	WD 4
	Repeat	160 – 200	41	WD 5
	Repeat	204 – 244	41	WD 6
	Repeat	246 – 285	40	WD 7
	Repeat	289 – 332	44	WD 8
Amino acid modifications
	Modified residue	382	1	Phosphothreonine; by PLK1 Ref.8
	Modified residue	397	1	Phosphoserine; by PLK1 Ref.8
	Modified residue	411	1	Phosphoserine Ref.6
	Modified residue	426	1	Phosphoserine; by PLK1 Ref.8
	Modified residue	516	1	Phosphoserine Ref.7 Ref.9
	Modified residue	550	1	Phosphothreonine; by CDK1 Ref.8
	Modified residue	637	1	Phosphoserine; by PLK1 Ref.8
Natural variations
	Alternative sequence	1 – 89	89	Missing in isoform 2.		VSP_043411
Experimental info
	Sequence conflict	170	1	Y → C in BAC04099. Ref.1
	Sequence conflict	409	1	M → V in BAC04099. Ref.1
	Sequence conflict	649	1	L → P in BAC04099. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 12817A567C13098B	FASTA	660	71,966
	10 20 30 40 50 60 MQENLRFASS GDDIKIWDAS SMTLVDKFNP HTSPHGISSI CWSSNNNFLV TASSSGDKIV 70 80 90 100 110 120 VSSCKCKPVP LLELAEGQKQ TCVNLNSTSM YLVSGGLNNT VNIWDLKSKR VHRSLKDHKD 130 140 150 160 170 180 QVTCVTYNWN DCYIASGSLS GEIILHSVTT NLSSTPFGHG SNQSVRHLKY SLFKKSLLGS 190 200 210 220 230 240 VSDNGIVTLW DVNSQSPYHN FDSVHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK 250 260 270 280 290 300 LVKTLVADTP LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ 310 320 330 340 350 360 YSTVLTKSSL NKGCSNKPTT VNKRSVNVNA ASGGVQNSGI VREAPATSIA TVLPQPMTSA 370 380 390 400 410 420 MGKGTVAVQE KAGLPRSINT DTLSKETDSG KNQDFSSFDD TGKSSLGDMF SPIRDDAVVN 430 440 450 460 470 480 KGSDESIGKG DGFDFLPQLN SVFPPRKNPV TSSTSVLHSS PLNVFMGSPG KEENENRDLT 490 500 510 520 530 540 AESKKIYMGK QESKDSFKQL AKLVTSGAES GNLNTSPSSN QTRNSEKFEK PENEIEAQLI 550 560 570 580 590 600 CEPPINGSST PNPKIASSVT AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL 610 620 630 640 650 660 DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF
	Isoform 2 [UniParc]. Checksum: 1FBE4E895B36789B	FASTA	571	62,379
	10 20 30 40 50 60 MYLVSGGLNN TVNIWDLKSK RVHRSLKDHK DQVTCVTYNW NDCYIASGSL SGEIILHSVT 70 80 90 100 110 120 TNLSSTPFGH GSNQSVRHLK YSLFKKSLLG SVSDNGIVTL WDVNSQSPYH NFDSVHKAPA 130 140 150 160 170 180 SGICFSPVNE LLFVTIGLDK RIILYDTSSK KLVKTLVADT PLTAVDFMPD GATLAIGSSR 190 200 210 220 230 240 GKIYQYDLRM LKSPVKTISA HKTSVQCIAF QYSTVLTKSS LNKGCSNKPT TVNKRSVNVN 250 260 270 280 290 300 AASGGVQNSG IVREAPATSI ATVLPQPMTS AMGKGTVAVQ EKAGLPRSIN TDTLSKETDS 310 320 330 340 350 360 GKNQDFSSFD DTGKSSLGDM FSPIRDDAVV NKGSDESIGK GDGFDFLPQL NSVFPPRKNP 370 380 390 400 410 420 VTSSTSVLHS SPLNVFMGSP GKEENENRDL TAESKKIYMG KQESKDSFKQ LAKLVTSGAE 430 440 450 460 470 480 SGNLNTSPSS NQTRNSEKFE KPENEIEAQL ICEPPINGSS TPNPKIASSV TAGVASSLSE 490 500 510 520 530 540 KIADSIGNNR QNAPLTSIQI RFIQNMIQET LDDFREACHR DIVNLQVEMI KQFHMQLNEM 550 560 570 HSLLERYSVN EGLVAEIERL REENKRLRAH F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis, Thymus and Tongue.
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[5]	"FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle." Zhu H., Coppinger J.A., Jang C.-Y., Yates J.R. III, Fang G. J. Cell Biol. 183:835-848(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FAM29A, SUBCELLULAR LOCATION, FUNCTION.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gammaTuRC to the centrosome." Zhang X., Chen Q., Feng J., Hou J., Yang F., Liu J., Jiang Q., Zhang C. J. Cell Sci. 122:2240-2251(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT THR-550 BY CDK1, PHOSPHORYLATION AT THR-382; SER-397; SER-426 AND SER-637 BY PLK1.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK093221 mRNA. Translation: BAC04099.1. AK303656 mRNA. Translation: BAG64657.1. AK315821 mRNA. Translation: BAF98712.1. AC007564 Genomic DNA. No translation available. AC013417 Genomic DNA. No translation available. CH471054 Genomic DNA. Translation: EAW97577.1. CH471054 Genomic DNA. Translation: EAW97579.1. BC027605 mRNA. Translation: AAH27605.1.
IPI	IPI00169345. IPI00789363.
RefSeq	NP_001128647.1. NM_001135175.1. NP_001128648.1. NM_001135176.1. NP_001128649.1. NM_001135177.1. NP_690869.1. NM_152905.3.
UniGene	Hs.740471.
3D structure databases
ProteinModelPortal	Q8NHV4.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48838N.
IntAct	Q8NHV4. 6 interactions.
STRING	9606.ENSP00000407964.
PTM databases
PhosphoSite	Q8NHV4.
Polymorphism databases
DMDM	74762597.
Proteomic databases
PaxDb	Q8NHV4.
PRIDE	Q8NHV4.
Protocols and materials databases
DNASU	121441.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000266742; ENSP00000266742; ENSG00000139350. ENST00000411739; ENSP00000411307; ENSG00000139350. ENST00000429527; ENSP00000404978; ENSG00000139350. ENST00000457368; ENSP00000407964; ENSG00000139350.
GeneID	121441.
KEGG	hsa:121441.
UCSC	uc001teu.4. human.
Organism-specific databases
CTD	121441.
GeneCards	GC12P097301.
HGNC	HGNC:7723. NEDD1.
HPA	HPA038591.
MIM	600372. gene.
neXtProt	NX_Q8NHV4.
PharmGKB	PA31531.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000034642.
HOVERGEN	HBG055623.
InParanoid	Q8NHV4.
KO	K16547.
OrthoDB	EOG4NZTSQ.
PhylomeDB	Q8NHV4.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
ArrayExpress	Q8NHV4.
Bgee	Q8NHV4.
CleanEx	HS_NEDD1.
Genevestigator	Q8NHV4.
GermOnline	ENSG00000139350. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	121441.
NextBio	80736.
SOURCE	Search...

Entry information

Entry name

NEDD1_HUMAN

Accession

Primary (citable) accession number: Q8NHV4
Secondary accession number(s): B0AZN0, B4E145, Q8NA30

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.