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Protein

DnaJ homolog subfamily B member 8

Gene

DNAJB8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Efficient suppressor of aggregation and toxicity of disease-associated polyglutamine proteins.1 Publication

GO - Molecular functioni

  • chaperone binding Source: UniProtKB
  • protein binding involved in protein folding Source: MGI
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

  • chaperone-mediated protein folding Source: MGI
  • negative regulation of inclusion body assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 8
Gene namesi
Name:DNAJB8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:23699. DNAJB8.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi216 – 2161K → A: Significant loss of activity. 1 Publication

Organism-specific databases

PharmGKBiPA134881798.

Polymorphism and mutation databases

BioMutaiDNAJB8.
DMDMi27805461.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232DnaJ homolog subfamily B member 8PRO_0000071029Add
BLAST

Proteomic databases

EPDiQ8NHS0.
MaxQBiQ8NHS0.
PaxDbiQ8NHS0.
PeptideAtlasiQ8NHS0.
PRIDEiQ8NHS0.

PTM databases

iPTMnetiQ8NHS0.
PhosphoSiteiQ8NHS0.

Expressioni

Gene expression databases

BgeeiQ8NHS0.
CleanExiHS_DNAJB8.
GenevisibleiQ8NHS0. HS.

Organism-specific databases

HPAiHPA034733.

Interactioni

Subunit structurei

Interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.1 Publication

GO - Molecular functioni

  • chaperone binding Source: UniProtKB
  • protein binding involved in protein folding Source: MGI
  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127919. 13 interactions.
IntActiQ8NHS0. 1 interaction.
STRINGi9606.ENSP00000316053.

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi18 – 2912Combined sources
Turni32 – 343Combined sources
Helixi40 – 5718Combined sources
Helixi59 – 6810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMXNMR-A1-79[»]
ProteinModelPortaliQ8NHS0.
SMRiQ8NHS0. Positions 1-105.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NHS0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 6967JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi149 – 18537Ser-richAdd
BLAST

Domaini

The antiaggregation activity resides in the serine-rich region and the C-terminus.

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ8NHS0.
KOiK09514.
OMAiDRAGCDG.
OrthoDBiEOG7KSX9F.
PhylomeDBiQ8NHS0.
TreeFamiTF105142.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NHS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS
60 70 80 90 100
EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF
110 120 130 140 150
REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS
160 170 180 190 200
FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV
210 220 230
ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK
Length:232
Mass (Da):25,686
Last modified:October 1, 2002 - v1
Checksum:i058B180995B772F6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti153 – 1531M → L.
Corresponds to variant rs35948511 [ dbSNP | Ensembl ].
VAR_033881

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK125946 mRNA. Translation: BAG54269.1.
AK126068 mRNA. Translation: BAG54285.1.
CH471052 Genomic DNA. Translation: EAW79320.1.
BC029521 mRNA. Translation: AAH29521.1.
BC050288 mRNA. Translation: AAH50288.1.
CCDSiCCDS3048.1.
RefSeqiNP_699161.1. NM_153330.4.
XP_006713582.1. XM_006713519.2.
UniGeneiHs.518241.

Genome annotation databases

EnsembliENST00000319153; ENSP00000316053; ENSG00000179407.
ENST00000469083; ENSP00000417418; ENSG00000179407.
GeneIDi165721.
KEGGihsa:165721.
UCSCiuc003ekk.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK125946 mRNA. Translation: BAG54269.1.
AK126068 mRNA. Translation: BAG54285.1.
CH471052 Genomic DNA. Translation: EAW79320.1.
BC029521 mRNA. Translation: AAH29521.1.
BC050288 mRNA. Translation: AAH50288.1.
CCDSiCCDS3048.1.
RefSeqiNP_699161.1. NM_153330.4.
XP_006713582.1. XM_006713519.2.
UniGeneiHs.518241.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMXNMR-A1-79[»]
ProteinModelPortaliQ8NHS0.
SMRiQ8NHS0. Positions 1-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127919. 13 interactions.
IntActiQ8NHS0. 1 interaction.
STRINGi9606.ENSP00000316053.

PTM databases

iPTMnetiQ8NHS0.
PhosphoSiteiQ8NHS0.

Polymorphism and mutation databases

BioMutaiDNAJB8.
DMDMi27805461.

Proteomic databases

EPDiQ8NHS0.
MaxQBiQ8NHS0.
PaxDbiQ8NHS0.
PeptideAtlasiQ8NHS0.
PRIDEiQ8NHS0.

Protocols and materials databases

DNASUi165721.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319153; ENSP00000316053; ENSG00000179407.
ENST00000469083; ENSP00000417418; ENSG00000179407.
GeneIDi165721.
KEGGihsa:165721.
UCSCiuc003ekk.3. human.

Organism-specific databases

CTDi165721.
GeneCardsiDNAJB8.
HGNCiHGNC:23699. DNAJB8.
HPAiHPA034733.
MIMi611337. gene.
neXtProtiNX_Q8NHS0.
PharmGKBiPA134881798.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ8NHS0.
KOiK09514.
OMAiDRAGCDG.
OrthoDBiEOG7KSX9F.
PhylomeDBiQ8NHS0.
TreeFamiTF105142.

Miscellaneous databases

EvolutionaryTraceiQ8NHS0.
GenomeRNAii165721.
PROiQ8NHS0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8NHS0.
CleanExiHS_DNAJB8.
GenevisibleiQ8NHS0. HS.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "A DNAJB chaperone subfamily with HDAC-dependent activities suppresses toxic protein aggregation."
    Hageman J., Rujano M.A., van Waarde M.A., Kakkar V., Dirks R.P., Govorukhina N., Oosterveld-Hut H.M., Lubsen N.H., Kampinga H.H.
    Mol. Cell 37:355-369(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDACS, MUTAGENESIS OF LYS-216.
  5. "Solution structure of the J domain of DnaJ homolog subfamily B member 8."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-82.

Entry informationi

Entry nameiDNJB8_HUMAN
AccessioniPrimary (citable) accession number: Q8NHS0
Secondary accession number(s): B3KWV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.