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Q8NHQ1 (CEP70_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centrosomal protein of 70 kDa

Short name=Cep70
Alternative name(s):
p10-binding protein
Gene names
Name:CEP70
Synonyms:BITE
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.

Subunit structure

Directly interacts with tubulin-gamma; this interaction determines centrosomal localization. Ref.7

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Note: Localized at the center of the radial microtubule array in interphase and at the spindle poles during various stages of mitosis. Ref.6 Ref.7

Domain

The coiled-coil domains may be important for tubulin-gamma-binding and hence for centrosomal localization.

Sequence similarities

Contains 1 TPR repeat.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NHQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NHQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     551-554: SIIY → RYLF
     555-597: Missing.
Isoform 3 (identifier: Q8NHQ1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     213-597: Missing.
Isoform 4 (identifier: Q8NHQ1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q8NHQ1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     22-23: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Centrosomal protein of 70 kDa
PRO_0000089497

Regions

Repeat483 – 51634TPR
Coiled coil75 – 179105 Potential
Coiled coil254 – 32673 Potential

Natural variations

Alternative sequence1 – 1818Missing in isoform 4 and isoform 5.
VSP_042140
Alternative sequence22 – 232Missing in isoform 5.
VSP_042141
Alternative sequence213 – 597385Missing in isoform 3.
VSP_012745
Alternative sequence551 – 5544SIIY → RYLF in isoform 2.
VSP_012746
Alternative sequence555 – 59743Missing in isoform 2.
VSP_012747
Natural variant1351S → N. Ref.2 Ref.4 Ref.5
Corresponds to variant rs1673607 [ dbSNP | Ensembl ].
VAR_021177
Natural variant5371N → S.
Corresponds to variant rs35340237 [ dbSNP | Ensembl ].
VAR_061590

Experimental info

Sequence conflict521Missing in BAB14403. Ref.2
Sequence conflict2931Q → L in BAH12924. Ref.2
Sequence conflict3711Q → R in BAB14403. Ref.2
Sequence conflict3851Q → R in BAH13584. Ref.2
Sequence conflict3931V → A in BAH13584. Ref.2
Sequence conflict4711S → P in AAG35616. Ref.1
Sequence conflict4711S → P in AAG35791. Ref.1
Sequence conflict4781D → G in AAG35616. Ref.1
Sequence conflict4781D → G in AAG35791. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: 345EEEB9B1F0D6C7

FASTA59769,752
        10         20         30         40         50         60 
MFPVAPKPQD SSQPSDRLMT EKQQEEAEWE SINVLLMMHG LKPLSLVKRT DLKDLIIFDK 

        70         80         90        100        110        120 
QSSQRMRQNL KLLVEETSCQ QNMIQELIET NQQLRNELQL EQSRAANQEQ RANDLEQIME 

       130        140        150        160        170        180 
SVKSKIGELE DESLSRACHQ QNKIKDLQKE QKTLQVKCQH YKKKRTEQEE TIASLQMEVC 

       190        200        210        220        230        240 
RLKKEEEDRI VTQNRVFAYL CKRVPHTVLD RQLLCLIDYY ESKIRKIHTQ RQYKEDESQS 

       250        260        270        280        290        300 
EEENDYRNLD ASPTYKGLLM SLQNQLKESK SKIDALSSEK LNLQKDLETR PTQHELRLYK 

       310        320        330        340        350        360 
QQVKKLEKAL KKNVKLQELI NHKKAEDTEK KDEPSKYNQQ QALIDQRYFQ VLCSINSIIH 

       370        380        390        400        410        420 
NPRAPVIIYK QTKGGVQNFN KDLVQDCGFE HLVPVIEMWA DQLTSLKDLY KSLKTLSAEL 

       430        440        450        460        470        480 
VPWLNLKKQD ENEGIKVEDL LFIVDTMLEE VENKEKDSNM PHFQTLQAIV SHFQKLFDVP 

       490        500        510        520        530        540 
SLNGVYPRMN EVYTRLGEMN NAVRNLQELL ELDSSSSLCV LVSTVGKLCR LINEDVNEQV 

       550        560        570        580        590 
MQVLGPEDLQ SIIYKLEEHE EFFPAFQAFT NDLLEILEID DLDAIVPAVK KLKVLSY 

« Hide

Isoform 2 [UniParc].

Checksum: 995E0945339F20CA
Show »

FASTA55464,880
Isoform 3 [UniParc].

Checksum: 5E1C436C297C8CF3
Show »

FASTA21225,034
Isoform 4 [UniParc].

Checksum: AE654471BDE652DF
Show »

FASTA57967,770
Isoform 5 [UniParc].

Checksum: F4A66BB90BB75EAE
Show »

FASTA57767,513

References

« Hide 'large scale' references
[1]"Cloning and characterization of BITE: a binding partner of the cell cycle inhibitor p10."
Tschan M.P., Cajot J.-F., Grob T.J., Fey M.F., Tobler A.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Chronic myeloid leukemia cell.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 4 AND 5), VARIANT ASN-135.
Tissue: Teratocarcinoma and Testis.
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-135.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 3), VARIANT ASN-135.
Tissue: Skin and Testis.
[6]"Proteomic characterization of the human centrosome by protein correlation profiling."
Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.
Nature 426:570-574(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Lymphoblast.
[7]"CEP70 protein interacts with gamma-tubulin to localize at the centrosome and is critical for mitotic spindle assembly."
Shi X., Sun X., Liu M., Li D., Aneja R., Zhou J.
J. Biol. Chem. 286:33401-33408(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TUBULIN-GAMMA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF202146 mRNA. Translation: AAG35616.1.
AF289495 mRNA. Translation: AAG35791.1.
AK023098 mRNA. Translation: BAB14403.1.
AK299003 mRNA. Translation: BAH12924.1.
AK301913 mRNA. Translation: BAH13584.1.
AC020890 Genomic DNA. No translation available.
AC072045 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79062.1.
CH471052 Genomic DNA. Translation: EAW79063.1.
BC016050 mRNA. Translation: AAH16050.1.
BC030598 mRNA. Translation: AAH30598.1.
CCDSCCDS3102.1. [Q8NHQ1-1]
RefSeqNP_001275893.1. NM_001288964.1. [Q8NHQ1-4]
NP_001275894.1. NM_001288965.1. [Q8NHQ1-5]
NP_001275895.1. NM_001288966.1. [Q8NHQ1-2]
NP_001275896.1. NM_001288967.1.
NP_077817.2. NM_024491.3. [Q8NHQ1-1]
XP_005247863.1. XM_005247806.1. [Q8NHQ1-1]
UniGeneHs.531962.

3D structure databases

ProteinModelPortalQ8NHQ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123234. 37 interactions.
IntActQ8NHQ1. 42 interactions.
MINTMINT-1446081.
STRING9606.ENSP00000264982.

PTM databases

PhosphoSiteQ8NHQ1.

Polymorphism databases

DMDM84028943.

Proteomic databases

PaxDbQ8NHQ1.
PRIDEQ8NHQ1.

Protocols and materials databases

DNASU80321.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264982; ENSP00000264982; ENSG00000114107. [Q8NHQ1-1]
ENST00000464035; ENSP00000419743; ENSG00000114107. [Q8NHQ1-3]
ENST00000474781; ENSP00000419833; ENSG00000114107. [Q8NHQ1-4]
ENST00000481834; ENSP00000417465; ENSG00000114107. [Q8NHQ1-2]
ENST00000484888; ENSP00000419231; ENSG00000114107. [Q8NHQ1-1]
ENST00000542237; ENSP00000444128; ENSG00000114107. [Q8NHQ1-5]
GeneID80321.
KEGGhsa:80321.
UCSCuc003esl.3. human. [Q8NHQ1-1]
uc003esm.3. human. [Q8NHQ1-2]
uc003esn.3. human. [Q8NHQ1-3]
uc011bmk.2. human. [Q8NHQ1-5]

Organism-specific databases

CTD80321.
GeneCardsGC03M138213.
H-InvDBHIX0018484.
HGNCHGNC:29972. CEP70.
HPAHPA036941.
HPA036942.
MIM614310. gene.
neXtProtNX_Q8NHQ1.
PharmGKBPA128394729.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG68751.
HOGENOMHOG000290163.
HOVERGENHBG050899.
InParanoidQ8NHQ1.
KOK16802.
OMAINVLLMM.
OrthoDBEOG70PC06.
PhylomeDBQ8NHQ1.
TreeFamTF330986.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.

Gene expression databases

ArrayExpressQ8NHQ1.
BgeeQ8NHQ1.
CleanExHS_CEP70.
GenevestigatorQ8NHQ1.

Family and domain databases

InterProIPR013026. TPR-contain_dom.
IPR019734. TPR_repeat.
[Graphical view]
PROSITEPS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCEP70.
GenomeRNAi80321.
NextBio70839.
PROQ8NHQ1.
SOURCESearch...

Entry information

Entry nameCEP70_HUMAN
AccessionPrimary (citable) accession number: Q8NHQ1
Secondary accession number(s): B7Z5I8 expand/collapse secondary AC list , B7Z7E8, D3DNE9, F5GZX8, Q96B31, Q9H2C3, Q9H2Z1, Q9H940
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM