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Protein

Putative phospholipase B-like 2

Gene

PLBD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative phospholipase.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Protein family/group databases

MEROPSiC95.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative phospholipase B-like 2 (EC:3.1.1.-)
Alternative name(s):
76 kDa protein
Short name:
p76
LAMA-like protein 2
Lamina ancestor homolog 2
Phospholipase B domain-containing protein 2
Cleaved into the following 2 chains:
Gene namesi
Name:PLBD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:27283. PLBD2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • lysosomal lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164724624.

Polymorphism and mutation databases

BioMutaiPLBD2.
DMDMi224471847.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 41411 PublicationAdd
BLAST
Chaini42 – 589548Putative phospholipase B-like 2PRO_0000286110Add
BLAST
Chaini42 – 243202Putative phospholipase B-like 2 32 kDa formPRO_0000314074Add
BLAST
Chaini244 – 589346Putative phospholipase B-like 2 45 kDa formPRO_0000314075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)1 Publication
Glycosylationi110 – 1101N-linked (GlcNAc...)1 Publication
Disulfide bondi142 ↔ 152By similarity
Glycosylationi231 – 2311N-linked (GlcNAc...)1 Publication
Glycosylationi436 – 4361N-linked (GlcNAc...)1 Publication
Glycosylationi465 – 4651N-linked (GlcNAc...)2 Publications
Disulfide bondi492 ↔ 495By similarity
Glycosylationi515 – 5151N-linked (GlcNAc...)1 Publication

Post-translational modificationi

The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
Glycosylated; contains mannose 6-phosphate sugars.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8NHP8.
MaxQBiQ8NHP8.
PaxDbiQ8NHP8.
PeptideAtlasiQ8NHP8.
PRIDEiQ8NHP8.

PTM databases

iPTMnetiQ8NHP8.
PhosphoSiteiQ8NHP8.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in heart, brain and liver.1 Publication

Gene expression databases

BgeeiQ8NHP8.
ExpressionAtlasiQ8NHP8. baseline and differential.
GenevisibleiQ8NHP8. HS.

Organism-specific databases

HPAiHPA017163.

Interactioni

Subunit structurei

Interacts with IGF2R.1 Publication

Protein-protein interaction databases

BioGridi128208. 14 interactions.
IntActiQ8NHP8. 9 interactions.
STRINGi9606.ENSP00000280800.

Structurei

3D structure databases

ProteinModelPortaliQ8NHP8.
SMRiQ8NHP8. Positions 56-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase B-like family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOGENOMiHOG000006903.
HOVERGENiHBG096591.
InParanoidiQ8NHP8.
OMAiTNAIRET.
OrthoDBiEOG7D2FD5.
PhylomeDBiQ8NHP8.
TreeFamiTF315042.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NHP8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGQMYCYPG SHLARALTRA LALALVLALL VGPFLSGLAG AIPAPGGRWA
60 70 80 90 100
RDGQVPPASR SRSVLLDVSA GQLLMVDGRH PDAVAWANLT NAIRETGWAF
110 120 130 140 150
LELGTSGQYN DSLQAYAAGV VEAAVSEELI YMHWMNTVVN YCGPFEYEVG
160 170 180 190 200
YCERLKSFLE ANLEWMQEEM ESNPDSPYWH QVRLTLLQLK GLEDSYEGRV
210 220 230 240 250
SFPAGKFTIK PLGFLLLQLS GDLEDLELAL NKTKIKPSLG SGSCSALIKL
260 270 280 290 300
LPGQSDLLVA HNTWNNYQHM LRVIKKYWLQ FREGPWGDYP LVPGNKLVFS
310 320 330 340 350
SYPGTIFSCD DFYILGSGLV TLETTIGNKN PALWKYVRPR GCVLEWVRNI
360 370 380 390 400
VANRLASDGA TWADIFKRFN SGTYNNQWMI VDYKAFIPGG PSPGSRVLTI
410 420 430 440 450
LEQIPGMVVV ADKTSELYQK TYWASYNIPS FETVFNASGL QALVAQYGDW
460 470 480 490 500
FSYDGSPRAQ IFRRNQSLVQ DMDSMVRLMR YNDFLHDPLS LCKACNPQPN
510 520 530 540 550
GENAISARSD LNPANGSYPF QALRQRSHGG IDVKVTSMSL ARILSLLAAS
560 570 580
GPTWDQVPPF QWSTSPFSGL LHMGQPDLWK FAPVKVSWD
Length:589
Mass (Da):65,472
Last modified:March 3, 2009 - v2
Checksum:iD0D1BACCFBF756BB
GO
Isoform 2 (identifier: Q8NHP8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     374-405: Missing.

Note: No experimental confirmation available.
Show »
Length:557
Mass (Da):61,885
Checksum:i6F9D325421593760
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti521 – 5211Q → K in AAH30618 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti54 – 541Q → P.
Corresponds to variant rs7965471 [ dbSNP | Ensembl ].
VAR_062187
Natural varianti524 – 5241R → C.
Corresponds to variant rs12425042 [ dbSNP | Ensembl ].
VAR_032075

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei374 – 40532Missing in isoform 2. 1 PublicationVSP_055623Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010178 Genomic DNA. No translation available.
BC030618 mRNA. Translation: AAH30618.1.
BC071832 mRNA. No translation available.
CCDSiCCDS53834.1. [Q8NHP8-2]
CCDS9168.1. [Q8NHP8-1]
RefSeqiNP_001153199.1. NM_001159727.1. [Q8NHP8-2]
NP_775813.2. NM_173542.3. [Q8NHP8-1]
UniGeneiHs.115896.

Genome annotation databases

EnsembliENST00000280800; ENSP00000280800; ENSG00000151176. [Q8NHP8-1]
ENST00000545182; ENSP00000443463; ENSG00000151176. [Q8NHP8-2]
GeneIDi196463.
KEGGihsa:196463.
UCSCiuc001tve.3. human. [Q8NHP8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010178 Genomic DNA. No translation available.
BC030618 mRNA. Translation: AAH30618.1.
BC071832 mRNA. No translation available.
CCDSiCCDS53834.1. [Q8NHP8-2]
CCDS9168.1. [Q8NHP8-1]
RefSeqiNP_001153199.1. NM_001159727.1. [Q8NHP8-2]
NP_775813.2. NM_173542.3. [Q8NHP8-1]
UniGeneiHs.115896.

3D structure databases

ProteinModelPortaliQ8NHP8.
SMRiQ8NHP8. Positions 56-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128208. 14 interactions.
IntActiQ8NHP8. 9 interactions.
STRINGi9606.ENSP00000280800.

Protein family/group databases

MEROPSiC95.001.

PTM databases

iPTMnetiQ8NHP8.
PhosphoSiteiQ8NHP8.

Polymorphism and mutation databases

BioMutaiPLBD2.
DMDMi224471847.

Proteomic databases

EPDiQ8NHP8.
MaxQBiQ8NHP8.
PaxDbiQ8NHP8.
PeptideAtlasiQ8NHP8.
PRIDEiQ8NHP8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000280800; ENSP00000280800; ENSG00000151176. [Q8NHP8-1]
ENST00000545182; ENSP00000443463; ENSG00000151176. [Q8NHP8-2]
GeneIDi196463.
KEGGihsa:196463.
UCSCiuc001tve.3. human. [Q8NHP8-1]

Organism-specific databases

CTDi196463.
GeneCardsiPLBD2.
HGNCiHGNC:27283. PLBD2.
HPAiHPA017163.
neXtProtiNX_Q8NHP8.
PharmGKBiPA164724624.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOGENOMiHOG000006903.
HOVERGENiHBG096591.
InParanoidiQ8NHP8.
OMAiTNAIRET.
OrthoDBiEOG7D2FD5.
PhylomeDBiQ8NHP8.
TreeFamiTF315042.

Miscellaneous databases

ChiTaRSiPLBD2. human.
GenomeRNAii196463.
NextBioi89484.
PROiQ8NHP8.

Gene expression databases

BgeeiQ8NHP8.
ExpressionAtlasiQ8NHP8. baseline and differential.
GenevisibleiQ8NHP8. HS.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis.
  3. "Biochemical characterization and lysosomal localization of the mannose-6-phosphate protein p76 (hypothetical protein LOC196463)."
    Jensen A.G., Chemali M., Chapel A., Kieffer-Jaquinod S., Jadot M., Garin J., Journet A.
    Biochem. J. 402:449-458(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-49 AND 244-250, GLYCOSYLATION AT ASN-88; ASN-110; ASN-231; ASN-436; ASN-465 AND ASN-515, INTERACTION WITH IGF2R, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-465.
    Tissue: Liver.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPLBL2_HUMAN
AccessioniPrimary (citable) accession number: Q8NHP8
Secondary accession number(s): F5H5E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 3, 2009
Last modified: April 13, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.