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Protein

Aflatoxin B1 aldehyde reductase member 4

Gene

AKR7L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441NADPBy similarity
Active sitei49 – 491Proton donorBy similarity
Sitei77 – 771Lowers pKa of active site TyrBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei169 – 1691NADPBy similarity
Binding sitei222 – 2221NADPBy similarity
Binding sitei232 – 2321SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 1442NADPBy similarity
Nucleotide bindingi198 – 20811NADPBy similarityAdd
BLAST
Nucleotide bindingi290 – 2989NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-HSA-5423646. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
Aflatoxin B1 aldehyde reductase member 4 (EC:1.-.-.-)
Alternative name(s):
AFB1 aldehyde reductase 3
Short name:
AFB1-AR 3
Aldoketoreductase 7-like
Gene namesi
Name:AKR7L
Synonyms:AFAR3, AKR7A4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24056. AKR7L.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164715300.

Polymorphism and mutation databases

DMDMi294862545.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Aflatoxin B1 aldehyde reductase member 4PRO_0000070379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei85 – 851PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NHP1.
PRIDEiQ8NHP1.

PTM databases

iPTMnetiQ8NHP1.
PhosphoSiteiQ8NHP1.

Expressioni

Tissue specificityi

Mainly expressed in uterus.1 Publication

Gene expression databases

BgeeiQ8NHP1.

Structurei

3D structure databases

ProteinModelPortaliQ8NHP1.
SMRiQ8NHP1. Positions 9-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000250286.
HOVERGENiHBG050576.
InParanoidiQ8NHP1.
OMAiGMYSATT.
PhylomeDBiQ8NHP1.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NHP1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFLYS
60 70 80 90 100
DGQSETILGG LGLRMGSSDC RVKIATKANP WIGNSLKPDS VRSQLETSLK
110 120 130 140 150
RLQCPUVDLF YLHAPDHSAP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV
160 170 180 190 200
AEICTLCKSN GWILPTVYQG MYSATTRQVE TELFPCLRHF GLRFYAYNPL
210 220 230 240 250
AGGLLTGKYK YEDKDGKQPV GRFFGTQWAE IYRNHFWKEH HFEGIALVEK
260 270 280 290 300
ALQAAYGASA PSMTSAALRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA
310 320 330
AAEEGPLEPA VVDAFNQAWH LFAHECPNYF I
Length:331
Mass (Da):36,964
Last modified:April 20, 2010 - v6
Checksum:i6311BEC97EE899F2
GO
Isoform 2 (identifier: Q8NHP1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-178: Missing.
     179-235: VETELFPCLR...TQWAEIYRNH → MHLTTAPRWK...TAGSCPLCTS

Show »
Length:153
Mass (Da):16,724
Checksum:i3C29E1ADB792AD73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861C → Y in CAC79668 (PubMed:12879023).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti255 – 2551A → T.1 Publication
Corresponds to variant rs2235795 [ dbSNP | Ensembl ].
VAR_046190
Natural varianti322 – 3221F → V.
Corresponds to variant rs2982534 [ dbSNP | Ensembl ].
VAR_046191

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 178178Missing in isoform 2. 1 PublicationVSP_035173Add
BLAST
Alternative sequencei179 – 23557VETEL…IYRNH → MHLTTAPRWKRHCVPATSCT RRASSWSLASPTMPPGKWPR SVPSARATAGSCPLCTS in isoform 2. 1 PublicationVSP_035174Add
BLAST

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei106 – 1061SelenocysteineCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035413 Genomic DNA. No translation available.
BC035351 mRNA. Translation: AAH35351.1.
AJ278012 mRNA. Translation: CAC79668.1.
UniGeneiHs.662342.

Genome annotation databases

EnsembliENST00000420396; ENSP00000406430; ENSG00000211454.
UCSCiuc057cwj.1. human. [Q8NHP1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035413 Genomic DNA. No translation available.
BC035351 mRNA. Translation: AAH35351.1.
AJ278012 mRNA. Translation: CAC79668.1.
UniGeneiHs.662342.

3D structure databases

ProteinModelPortaliQ8NHP1.
SMRiQ8NHP1. Positions 9-330.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ8NHP1.
PhosphoSiteiQ8NHP1.

Polymorphism and mutation databases

DMDMi294862545.

Proteomic databases

EPDiQ8NHP1.
PRIDEiQ8NHP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000420396; ENSP00000406430; ENSG00000211454.
UCSCiuc057cwj.1. human. [Q8NHP1-1]

Organism-specific databases

GeneCardsiAKR7L.
HGNCiHGNC:24056. AKR7L.
MIMi608478. gene.
neXtProtiNX_Q8NHP1.
PharmGKBiPA164715300.
GenAtlasiSearch...

Phylogenomic databases

HOGENOMiHOG000250286.
HOVERGENiHBG050576.
InParanoidiQ8NHP1.
OMAiGMYSATT.
PhylomeDBiQ8NHP1.

Enzyme and pathway databases

ReactomeiR-HSA-5423646. Aflatoxin activation and detoxification.

Miscellaneous databases

NextBioi13641911.
PROiQ8NHP1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8NHP1.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon.
  3. "Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells."
    Praml C., Savelyeva L., Schwab M.
    Oncogene 22:4765-4773(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-331, VARIANT THR-255, TISSUE SPECIFICITY, PROBABLE SELENOCYSTEINE AT SEC-106.
    Tissue: Uterus.

Entry informationi

Entry nameiARK74_HUMAN
AccessioniPrimary (citable) accession number: Q8NHP1
Secondary accession number(s): Q5U614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: April 20, 2010
Last modified: March 16, 2016
This is version 110 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.