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Q8NHM4 (TRY6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative trypsin-6

EC=3.4.21.4
Alternative name(s):
Serine protease 3 pseudogene 2
Trypsinogen C
Gene names
Name:PRSS3P2
Synonyms:T6, TRY6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceUncertain

General annotation (Comments)

Function

May regulate cell migration. Ref.3

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secreted Potential.

Tissue specificity

Overexpressed in metastasing in non small cell lung tumors, leading to an enhanced cell migration. Ref.3

Sequence similarities

Belongs to the peptidase S1 family. Tryptase subfamily.

Contains 1 peptidase S1 domain.

Caution

Could be the product of a pseudogene. However, some data suggest that it could be a protein-coding gene (Ref.3).

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendothelial cell migration

Inferred from mutant phenotype Ref.3. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Chain16 – 247232Putative trypsin-6
PRO_0000337059

Regions

Domain24 – 244221Peptidase S1
Compositional bias19 – 224Poly-Asp

Sites

Active site631Charge relay system By similarity
Active site1071Charge relay system By similarity
Active site2001Charge relay system By similarity
Metal binding751Calcium By similarity
Metal binding771Calcium; via carbonyl oxygen By similarity
Metal binding801Calcium; via carbonyl oxygen By similarity
Metal binding851Calcium By similarity
Site1941Required for specificity By similarity

Amino acid modifications

Disulfide bond48 ↔ 64 By similarity
Disulfide bond139 ↔ 206 By similarity
Disulfide bond171 ↔ 185 By similarity
Disulfide bond196 ↔ 220 By similarity

Experimental info

Sequence conflict1031I → T in AAC80208. Ref.2
Sequence conflict1821N → K in AAC80208. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8NHM4 [UniParc].

Last modified February 19, 2014. Version 2.
Checksum: 4F93CA1C93803264

FASTA24726,537
        10         20         30         40         50         60 
MNPLLILAFV GAAVAVPFDD DDKIVGGYTC EENSVPYQVS LNSGSHFCGG SLISEQWVVS 

        70         80         90        100        110        120 
AGHCYKPHIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN RIILNNDIML IKLSTPAVIN 

       130        140        150        160        170        180 
AHVSTISLPT APPAAGTECL ISGWGNTLSS GADYPDELQC LDAPVLTQAK CKASYPLKIT 

       190        200        210        220        230        240 
SNMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGIVSWGYGC AQKRRPGVYT KVYNYVDWIK 


DTIAANS 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The complete 685-kilobase DNA sequence of the human beta T cell receptor locus."
Rowen L., Koop B.F., Hood L.
Science 272:1755-1762(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"S100 family members and trypsinogens are predictors of distant metastasis and survival in early-stage non-small cell lung cancer."
Diederichs S., Bulk E., Steffen B., Ji P., Tickenbrock L., Lang K., Zanker K.S., Metzger R., Schneider P.M., Gerke V., Thomas M., Berdel W.E., Serve H., Muller-Tidow C.
Cancer Res. 64:5564-5569(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC231380 Genomic DNA. No translation available.
L36092 Genomic DNA. Translation: AAC80208.1.
UniGeneHs.449281.
Hs.728780.

3D structure databases

ProteinModelPortalQ8NHM4.
SMRQ8NHM4. Positions 24-247.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8NHM4. 1 interaction.

Protein family/group databases

MEROPSS01.298.

PTM databases

PhosphoSiteQ8NHM4.

Polymorphism databases

DMDM74760358.

Proteomic databases

PRIDEQ8NHM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCuc011ksq.2. human.

Organism-specific databases

HGNCHGNC:43788. PRSS3P2.
neXtProtNX_Q8NHM4.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHOG000251820.
HOVERGENHBG013304.

Gene expression databases

GenevestigatorQ8NHM4.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio35541312.

Entry information

Entry nameTRY6_HUMAN
AccessionPrimary (citable) accession number: Q8NHM4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 19, 2014
Last modified: June 11, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM