Q8NHM4 (TRY6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative trypsin-6
EC=3.4.21.4

Alternative name(s):
Trypsinogen C

Gene names

Name:	TRY6
Synonyms:	T6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted Potential.
Tissue specificity	Overexpressed in metastasing in non small cell lung tumors, leading to an enhanced cell migration. Ref.3
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.
Caution	Could be the product of a pseudogene. Although being similar to trypsinogen, it appears to be a transcribed pseudogene. ESTs support its transcription, but expression of its predicted protein has not been observed.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endothelial cell migration Inferred from mutant phenotype Ref.3. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 247	232	Putative trypsin-6		PRO_0000337059
Regions
	Domain	24 – 244	221	Peptidase S1
	Compositional bias	19 – 22	4	Poly-Asp
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	139 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NHM4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6B9F55B6A4F757BE	FASTA	247	26,539
	10 20 30 40 50 60 MNPLLILAFV GAAVAVPFDD DDKIVGGYTC EENSVPYQVS LNSGSHFCGG SLISEQWVVS 70 80 90 100 110 120 AGHCYKPHIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN RITLNNDIML IKLSTPAVIN 130 140 150 160 170 180 AHVSTISLPT APPAAGTECL ISGWGNTLSS GADYPDELQC LDAPVLTQAK CKASYPLKIT 190 200 210 220 230 240 SKMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGIVSWGYGC AQKRRPGVYT KVYNYVDWIK DTIAANS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The complete 685-kilobase DNA sequence of the human beta T cell receptor locus." Rowen L., Koop B.F., Hood L. Science 272:1755-1762(1996) [PubMed: 8650574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"S100 family members and trypsinogens are predictors of distant metastasis and survival in early-stage non-small cell lung cancer." Diederichs S., Bulk E., Steffen B., Ji P., Tickenbrock L., Lang K., Zanker K.S., Metzger R., Schneider P.M., Gerke V., Thomas M., Berdel W.E., Serve H., Muller-Tidow C. Cancer Res. 64:5564-5569(2004) [PubMed: 15313892] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC231380 Genomic DNA. No translation available. L36092 Genomic DNA. Translation: AAC80208.1.
IPI	IPI00169276.
UniGene	Hs.449281.
3D structure databases
HSSP	HSSP built from PDB template 1H4W based on UniProtKB P35030.
ProteinModelPortal	Q8NHM4.
SMR	Q8NHM4. Positions 24-247.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NHM4. 1 interaction.
STRING	Q8NHM4.
Protein family/group databases
MEROPS	S01.298.
Polymorphism databases
DMDM	74760358.
Proteomic databases
PRIDE	Q8NHM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000290968; ENSP00000290968; ENSG00000204983. ENST00000361585; ENSP00000355222; ENSG00000204983.
UCSC	uc003wao.1. human.
Organism-specific databases
GeneCards	GC07P142478.
neXtProt	NX_Q8NHM4.
Phylogenomic databases
eggNOG	prNOG16001.
GeneTree	ENSGT00580000081320.
HOVERGEN	HBG013304.
Gene expression databases
ArrayExpress	Q8NHM4.
Genevestigator	Q8NHM4.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY6_HUMAN

Accession

Primary (citable) accession number: Q8NHM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.