Q8NG68 (TTL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tubulin--tyrosine ligase
Short name=TTL
EC=6.3.2.25

Gene names

Name:

TTL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin By similarity.
Catalytic activity	ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the tubulin--tyrosine ligase family. Contains 1 TTL domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Nucleotide-binding Potassium
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tubulin-tyrosine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Tubulin--tyrosine ligase		PRO_0000212434
Regions
	Domain	3 – 370	368	TTL
Experimental info
	Sequence conflict	245	1	I → V in CAD98091. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q8NG68 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 7A13E2C28E1AD6EA	FASTA	377	43,212
	10 20 30 40 50 60 MYTFVVRDEN SSVYAEVSRL LLATGHWKRL RRDNPRFNLM LGERNRLPFG RLGHEPGLVQ 70 80 90 100 110 120 LVNYYRGADK LCRKASLVKL IKTSPELAES CTWFPESYVI YPTNLKTPVA PAQNGIQPPI 130 140 150 160 170 180 SNSRTDEREF FLASYNRKKE DGEGNVWIAK SSAGAKGEGI LISSEASELL DFIDNQGQVH 190 200 210 220 230 240 VIQKYLEHPL LLEPGHRKFD IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL 250 260 270 280 290 300 TNHCIQKEYS KNYGKYEEGN EMFFKEFNQY LTSALNITLE SSILLQIKHI IRNCLLSVEP 310 320 330 340 350 360 AISTKHLPYQ SFQLFGFDFM VDEELKVWLI EVNGAPACAQ KLYAELCQGI VDIAISSVFP 370 PPDVEQPQTQ PAAFIKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homo sapiens tubulin tyrosine ligase mRNA, complete cds." Miyazaki K., Okamoto Y., Kato C., Sakamoto M., Ohira M., Morohashi A., Nakagawara A. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 205-377. Tissue: Uterus.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB071393 mRNA. Translation: BAC06832.2. AC012442 Genomic DNA. Translation: AAX81998.1. CH471217 Genomic DNA. Translation: EAW73589.1. BC036819 mRNA. Translation: AAH36819.1. BX538316 mRNA. Translation: CAD98091.1.
IPI	IPI00166611.
RefSeq	NP_714923.1. NM_153712.4.
UniGene	Hs.358997.
3D structure databases
ProteinModelPortal	Q8NG68.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NG68.
PTM databases
PhosphoSite	Q8NG68.
Polymorphism databases
DMDM	47117358.
Proteomic databases
PeptideAtlas	Q8NG68.
PRIDE	Q8NG68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233336; ENSP00000233336; ENSG00000114999.
GeneID	150465.
KEGG	hsa:150465.
UCSC	uc002thu.1. human.
Organism-specific databases
CTD	150465.
GeneCards	GC02P113239.
H-InvDB	HIX0200272.
HGNC	HGNC:21586. TTL.
MIM	608291. gene.
neXtProt	NX_Q8NG68.
PharmGKB	PA134934330.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00600000084169.
HOGENOM	HBG717360.
HOVERGEN	HBG016579.
InParanoid	Q8NG68.
OMA	HLTNHCL.
OrthoDB	EOG412M5S.
PhylomeDB	Q8NG68.
Enzyme and pathway databases
BRENDA	6.3.2.25. 2681.
Gene expression databases
ArrayExpress	Q8NG68.
Bgee	Q8NG68.
CleanEx	HS_TTL.
Genevestigator	Q8NG68.
GermOnline	ENSG00000114999. Homo sapiens.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR004344. Tub_tyr_ligase. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K06047.
PANTHER	PTHR12241. Tub_tyr_ligase. 1 hit.
Pfam	PF03133. TTL. 1 hit. [Graphical view]
PROSITE	PS51221. TTL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	86445.
SOURCE	Search...

Entry information

Entry name

TTL_HUMAN

Accession

Primary (citable) accession number: Q8NG68
Secondary accession number(s): Q585T3, Q7Z302, Q8N426

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2004
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.