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Protein

Serine/threonine-protein kinase Nek11

Gene

NEK11

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mn2+2 Publications, Mg2+2 Publications

Enzyme regulationi

Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1 Publication1
Active sitei158Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 43ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • G2 DNA damage checkpoint Source: UniProtKB
  • histone phosphorylation Source: CACAO
  • intracellular signal transduction Source: UniProtKB
  • intra-S DNA damage checkpoint Source: UniProtKB
  • mitotic cell cycle phase transition Source: CACAO
  • protein phosphorylation Source: UniProtKB
  • regulation of mitotic cell cycle phase transition Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03787-MONOMER.
SignaLinkiQ8NG66.
SIGNORiQ8NG66.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek11 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 11
Short name:
NimA-related protein kinase 11
Gene namesi
Name:NEK11Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:18593. NEK11.

Subcellular locationi

  • Nucleus
  • Nucleusnucleolus

  • Note: Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.

GO - Cellular componenti

  • nucleolus Source: UniProtKB
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61K → R: Loss of kinase activity. 1 Publication1

Organism-specific databases

DisGeNETi79858.
OpenTargetsiENSG00000114670.
PharmGKBiPA38595.

Chemistry databases

ChEMBLiCHEMBL5638.
DrugBankiDB08912. Dabrafenib.
GuidetoPHARMACOLOGYi2116.

Polymorphism and mutation databases

BioMutaiNEK11.
DMDMi313104142.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864381 – 645Serine/threonine-protein kinase Nek11Add BLAST645

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273Phosphoserine; by CHEK12 Publications1

Post-translational modificationi

Phosphorylated by NEK2. Phosphorylation at Ser-273 is important for its activation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8NG66.
PeptideAtlasiQ8NG66.
PRIDEiQ8NG66.

PTM databases

iPTMnetiQ8NG66.
PhosphoSitePlusiQ8NG66.

Expressioni

Tissue specificityi

Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.1 Publication

Developmental stagei

Predominantly expressed at S/G2/M phase.1 Publication

Gene expression databases

BgeeiENSG00000114670.
CleanExiHS_NEK11.
ExpressionAtlasiQ8NG66. baseline and differential.
GenevisibleiQ8NG66. HS.

Organism-specific databases

HPAiHPA016908.

Interactioni

Subunit structurei

Interacts with isoform 1 of NEK2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NEK2P519555EBI-633195,EBI-633182
NEK2P51955-12EBI-633195,EBI-687792

Protein-protein interaction databases

BioGridi122947. 6 interactors.
IntActiQ8NG66. 3 interactors.
STRINGi9606.ENSP00000372857.

Chemistry databases

BindingDBiQ8NG66.

Structurei

3D structure databases

ProteinModelPortaliQ8NG66.
SMRiQ8NG66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST259

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili346 – 385Sequence analysisBy similarityAdd BLAST40

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000293260.
HOVERGENiHBG082016.
InParanoidiQ8NG66.
KOiK08857.
OrthoDBiEOG091G0FBH.
PhylomeDBiQ8NG66.
TreeFamiTF106472.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8NG66-1) [UniParc]FASTAAdd to basket
Also known as: Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK
60 70 80 90 100
AKRGEELKVL KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE
110 120 130 140 150
QDNFCIITEY CEGRDLDDKI QEYKQAGKIF PENQIIEWFI QLLLGVDYMH
160 170 180 190 200
ERRILHRDLK SKNVFLKNNL LKIGDFGVSR LLMGSCDLAT TLTGTPHYMS
210 220 230 240 250
PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI VLKIVEGDTP
260 270 280 290 300
SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE
310 320 330 340 350
MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL
360 370 380 390 400
QAADEKARKL KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM
410 420 430 440 450
EEKEEQPEGR LSCSPQDEDE ERWQGREEES DEPTLENLPE SQPIPSMDLH
460 470 480 490 500
ELESIVEDAT SDLGYHEIPE DPLVAEEYYA DAFDSYCEES DEEEEEIALE
510 520 530 540 550
RPEKEIRNEG SQPAYRTNQQ DSDIEALARC LENVLGCTSL DTKTITTMAE
560 570 580 590 600
DMSPGPPIFN SVMARTKMKR MRESAMQKLG TEVFEEVYNY LKRARHQNAS
610 620 630 640
EAEIRECLEK VVPQASDCFE VDQLLYFEEQ LLITMGKEPT LQNHL
Length:645
Mass (Da):74,192
Last modified:November 30, 2010 - v2
Checksum:i7D11AE1D89711DEC
GO
Isoform 21 Publication (identifier: Q8NG66-2) [UniParc]FASTAAdd to basket
Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     467-470: EIPE → ATHS
     471-645: Missing.

Show »
Length:470
Mass (Da):54,007
Checksum:iF52B82ED2B096FFB
GO
Isoform 3Curated (identifier: Q8NG66-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     467-482: EIPEDPLVAEEYYADA → GDCNLISLDEYWKNEK
     483-645: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:482
Mass (Da):55,519
Checksum:iFA6D89E50C0223A5
GO
Isoform 4 (identifier: Q8NG66-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     542-599: TKTITTMAED...YLKRARHQNA → QPCRSWGQKY...TSSCTLKSSC
     600-645: Missing.

Note: No experimental confirmation available.
Show »
Length:599
Mass (Da):68,880
Checksum:iD9080C265940829F
GO

Sequence cautioni

The sequence BAB15672 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040935108T → M in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs200709914dbSNPEnsembl.1
Natural variantiVAR_040936123Y → C.1 PublicationCorresponds to variant rs55806123dbSNPEnsembl.1
Natural variantiVAR_040937213S → L.1 PublicationCorresponds to variant rs55920129dbSNPEnsembl.1
Natural variantiVAR_040938263I → V.1 PublicationCorresponds to variant rs35567155dbSNPEnsembl.1
Natural variantiVAR_040939451E → K.1 PublicationCorresponds to variant rs35409692dbSNPEnsembl.1
Natural variantiVAR_040940488E → V.4 PublicationsCorresponds to variant rs3738000dbSNPEnsembl.1
Natural variantiVAR_040941492E → K in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs140599545dbSNPEnsembl.1
Natural variantiVAR_040942548M → T.1 PublicationCorresponds to variant rs55813244dbSNPEnsembl.1
Natural variantiVAR_033907562V → A.1 PublicationCorresponds to variant rs16836266dbSNPEnsembl.1
Natural variantiVAR_040943606E → K.1 PublicationCorresponds to variant rs55944737dbSNPEnsembl.1
Natural variantiVAR_040944617D → N in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs765563230dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051820467 – 482EIPED…YYADA → GDCNLISLDEYWKNEK in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_051821467 – 470EIPE → ATHS in isoform 2. 1 Publication4
Alternative sequenceiVSP_051822471 – 645Missing in isoform 2. 1 PublicationAdd BLAST175
Alternative sequenceiVSP_051823483 – 645Missing in isoform 3. 1 PublicationAdd BLAST163
Alternative sequenceiVSP_040080542 – 599TKTIT…RHQNA → QPCRSWGQKYLKRSIITSRE QGIRMLAKQRSASVWKKWCL KPATVLKWTSSCTLKSSC in isoform 4. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_040081600 – 645Missing in isoform 4. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071996 mRNA. Translation: BAC06350.1.
AB071997 mRNA. Translation: BAC06351.1.
AL833472 mRNA. Translation: CAI46114.1.
AC055733 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79213.1.
BC028587 mRNA. Translation: AAH28587.1.
AK027148 mRNA. Translation: BAB15672.1. Different initiation.
CCDSiCCDS3069.1. [Q8NG66-1]
CCDS46915.1. [Q8NG66-2]
CCDS54639.1. [Q8NG66-4]
CCDS82836.1. [Q8NG66-3]
RefSeqiNP_001139475.1. NM_001146003.1. [Q8NG66-4]
NP_001308149.1. NM_001321220.1. [Q8NG66-1]
NP_001308150.1. NM_001321221.1.
NP_001308151.1. NM_001321222.1.
NP_001308152.1. NM_001321223.1. [Q8NG66-3]
NP_001308153.1. NM_001321224.1.
NP_079076.3. NM_024800.4. [Q8NG66-1]
NP_665917.1. NM_145910.3. [Q8NG66-2]
XP_011511469.1. XM_011513167.2. [Q8NG66-1]
XP_016862702.1. XM_017007213.1. [Q8NG66-4]
XP_016862703.1. XM_017007214.1. [Q8NG66-4]
UniGeneiHs.657336.

Genome annotation databases

EnsembliENST00000356918; ENSP00000349389; ENSG00000114670. [Q8NG66-2]
ENST00000383366; ENSP00000372857; ENSG00000114670. [Q8NG66-1]
ENST00000507910; ENSP00000426662; ENSG00000114670. [Q8NG66-3]
ENST00000508196; ENSP00000421851; ENSG00000114670. [Q8NG66-1]
ENST00000510688; ENSP00000423458; ENSG00000114670. [Q8NG66-4]
ENST00000511262; ENSP00000425114; ENSG00000114670. [Q8NG66-2]
GeneIDi79858.
KEGGihsa:79858.
UCSCiuc003enx.4. human. [Q8NG66-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071996 mRNA. Translation: BAC06350.1.
AB071997 mRNA. Translation: BAC06351.1.
AL833472 mRNA. Translation: CAI46114.1.
AC055733 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79213.1.
BC028587 mRNA. Translation: AAH28587.1.
AK027148 mRNA. Translation: BAB15672.1. Different initiation.
CCDSiCCDS3069.1. [Q8NG66-1]
CCDS46915.1. [Q8NG66-2]
CCDS54639.1. [Q8NG66-4]
CCDS82836.1. [Q8NG66-3]
RefSeqiNP_001139475.1. NM_001146003.1. [Q8NG66-4]
NP_001308149.1. NM_001321220.1. [Q8NG66-1]
NP_001308150.1. NM_001321221.1.
NP_001308151.1. NM_001321222.1.
NP_001308152.1. NM_001321223.1. [Q8NG66-3]
NP_001308153.1. NM_001321224.1.
NP_079076.3. NM_024800.4. [Q8NG66-1]
NP_665917.1. NM_145910.3. [Q8NG66-2]
XP_011511469.1. XM_011513167.2. [Q8NG66-1]
XP_016862702.1. XM_017007213.1. [Q8NG66-4]
XP_016862703.1. XM_017007214.1. [Q8NG66-4]
UniGeneiHs.657336.

3D structure databases

ProteinModelPortaliQ8NG66.
SMRiQ8NG66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122947. 6 interactors.
IntActiQ8NG66. 3 interactors.
STRINGi9606.ENSP00000372857.

Chemistry databases

BindingDBiQ8NG66.
ChEMBLiCHEMBL5638.
DrugBankiDB08912. Dabrafenib.
GuidetoPHARMACOLOGYi2116.

PTM databases

iPTMnetiQ8NG66.
PhosphoSitePlusiQ8NG66.

Polymorphism and mutation databases

BioMutaiNEK11.
DMDMi313104142.

Proteomic databases

PaxDbiQ8NG66.
PeptideAtlasiQ8NG66.
PRIDEiQ8NG66.

Protocols and materials databases

DNASUi79858.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356918; ENSP00000349389; ENSG00000114670. [Q8NG66-2]
ENST00000383366; ENSP00000372857; ENSG00000114670. [Q8NG66-1]
ENST00000507910; ENSP00000426662; ENSG00000114670. [Q8NG66-3]
ENST00000508196; ENSP00000421851; ENSG00000114670. [Q8NG66-1]
ENST00000510688; ENSP00000423458; ENSG00000114670. [Q8NG66-4]
ENST00000511262; ENSP00000425114; ENSG00000114670. [Q8NG66-2]
GeneIDi79858.
KEGGihsa:79858.
UCSCiuc003enx.4. human. [Q8NG66-1]

Organism-specific databases

CTDi79858.
DisGeNETi79858.
GeneCardsiNEK11.
HGNCiHGNC:18593. NEK11.
HPAiHPA016908.
MIMi609779. gene.
neXtProtiNX_Q8NG66.
OpenTargetsiENSG00000114670.
PharmGKBiPA38595.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000293260.
HOVERGENiHBG082016.
InParanoidiQ8NG66.
KOiK08857.
OrthoDBiEOG091G0FBH.
PhylomeDBiQ8NG66.
TreeFamiTF106472.

Enzyme and pathway databases

BioCyciZFISH:HS03787-MONOMER.
SignaLinkiQ8NG66.
SIGNORiQ8NG66.

Miscellaneous databases

ChiTaRSiNEK11. human.
GenomeRNAii79858.
PROiQ8NG66.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114670.
CleanExiHS_NEK11.
ExpressionAtlasiQ8NG66. baseline and differential.
GenevisibleiQ8NG66. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEK11_HUMAN
AccessioniPrimary (citable) accession number: Q8NG66
Secondary accession number(s): A6NHD7
, Q5JPC0, Q8NG65, Q8TBY1, Q9H5F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.