Reviewed, UniProtKB/Swiss-Prot Q8NG66 (NEK11_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase Nek11
    EC=2.7.11.1
Alternative name(s):
    Never in mitosis A-related kinase 11
      Short name=NimA-related protein kinase 11

Gene names

Name:

NEK11

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible role in S-phase checkpoint mechanism. Ref.1 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1 Ref.5
Cofactor	Manganese or magnesium. Ref.1 Ref.5
Enzyme regulation	Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc. Ref.1 Ref.5
Subcellular location	Nucleus. Nucleus › nucleolus. Note: Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. Ref.1 Ref.5
Tissue specificity	Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Ref.1
Developmental stage	Predominantly expressed at S/G2/M phase. Ref.1
Post-translational modification	Phosphorylated. Ref.1 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAI46114.1 differs from that shown. Reason: Frameshift at position 573.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW intra-S DNA damage checkpoint Ref.5 Inferred from direct assay. Source: UniProtKB protein amino acid phosphorylation Ref.5 Inferred from direct assay. Source: UniProtKB protein kinase cascade Ref.5 Inferred from direct assay. Source: UniProtKB
Cellular component	nucleolus Ref.5 Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Ref.5 Inferred from direct assay. Source: UniProtKB identical protein binding Ref.5 Inferred from physical interaction. Source: IntAct magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Ref.5 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-633195,EBI-633195
NEK2	P51955	3	EBI-633195,EBI-633182
NEK2	P51955-1	2	EBI-633195,EBI-687792

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q8NG66-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q8NG66-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     467-470: EIPE → ATHS
     471-645: Missing.

Isoform 3 (identifier: Q8NG66-3)

The sequence of this isoform differs from the canonical sequence as follows:
     467-482: EIPEDPLVAEEYYADA → GDCNLISLDEYWKNEK
     483-645: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Serine/threonine-protein kinase Nek11		PRO_0000086438
Regions
	Domain	29 – 287	259	Protein kinase
	Nucleotide binding	35 – 43	9	ATP By similarity UniProtKB P51957
	Coiled coil	346 – 385	40	Potential UniProtKB P51957
Sites
	Active site	158	1	Proton acceptor By similarity UniProtKB P51957
	Binding site	61	1	ATP Ref.1
Natural variations
	Alternative sequence	467 – 482	16	EIPED…YYADA → GDCNLISLDEYWKNEK in isoform 3.		VSP_051820
	Alternative sequence	467 – 470	4	EIPE → ATHS in isoform 2. Ref.1		VSP_051821
	Alternative sequence	471 – 645	175	Missing in isoform 2. Ref.1		VSP_051822
	Alternative sequence	483 – 645	163	Missing in isoform 3.		VSP_051823
	Natural variant	108	1	T → M in a colorectal adenocarcinoma sample; somatic mutation. Ref.6		VAR_040935
	Natural variant	123	1	Y → C		VAR_040936
	Natural variant	213	1	S → L		VAR_040937
	Natural variant	263	1	I → V		VAR_040938
	Natural variant	451	1	E → K		VAR_040939
	Natural variant	488	1	V → E: dbSNP rs3738000. Ref.6		VAR_040940
	Natural variant	492	1	E → K in a colorectal adenocarcinoma sample; somatic mutation. Ref.6		VAR_040941
	Natural variant	548	1	M → T		VAR_040942
	Natural variant	562	1	V → A: dbSNP rs16836266. Ref.6		VAR_033907
	Natural variant	606	1	E → K		VAR_040943
	Natural variant	617	1	D → N in a colorectal adenocarcinoma sample; somatic mutation. Ref.6		VAR_040944
Experimental info
	Mutagenesis	61	1	K → R: Loss of kinase activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FE957E05C5FFB059	FASTA	645	74,162
	10 20 30 40 50 60 MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL 70 80 90 100 110 120 KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI 130 140 150 160 170 180 QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR 190 200 210 220 230 240 LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI 250 260 270 280 290 300 VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE 310 320 330 340 350 360 MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL 370 380 390 400 410 420 KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE 430 440 450 460 470 480 ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHEIPE DPLVAEEYYA 490 500 510 520 530 540 DAFDSYCVES DEEEEEIALE RPEKEIRNEG SQPAYRTNQQ DSDIEALARC LENVLGCTSL 550 560 570 580 590 600 DTKTITTMAE DMSPGPPIFN SVMARTKMKR MRESAMQKLG TEVFEEVYNY LKRARHQNAS 610 620 630 640 EAEIRECLEK VVPQASDCFE VDQLLYFEEQ LLITMGKEPT LQNHL
	Isoform 2 (Short). Checksum: F52B82ED2B096FFB	FASTA	470	54,007
	10 20 30 40 50 60 MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL 70 80 90 100 110 120 KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI 130 140 150 160 170 180 QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR 190 200 210 220 230 240 LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI 250 260 270 280 290 300 VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE 310 320 330 340 350 360 MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL 370 380 390 400 410 420 KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE 430 440 450 460 470 ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHATHS
	Isoform 3. Checksum: FA6D89E50C0223A5	FASTA	482	55,519
	10 20 30 40 50 60 MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL 70 80 90 100 110 120 KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI 130 140 150 160 170 180 QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR 190 200 210 220 230 240 LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI 250 260 270 280 290 300 VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE 310 320 330 340 350 360 MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL 370 380 390 400 410 420 KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE 430 440 450 460 470 480 ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHGDCN LISLDEYWKN EK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses." Noguchi K., Fukazawa H., Murakami Y., Uehara Y. J. Biol. Chem. 277:39655-39665(2002) [PubMed: 12154088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, ENZYME REGULATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-61. Tissue: Cervix carcinoma.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cervix.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 162-645 (ISOFORM 1). Tissue: Lung.
[5]	"Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells." Noguchi K., Fukazawa H., Murakami Y., Uehara Y. J. Biol. Chem. 279:32716-32727(2004) [PubMed: 15161910] [Abstract] Cited for: SUBCELLULAR LOCATION, ENZYME REGULATION.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-108; CYS-123; LEU-213; VAL-263; LYS-451; GLU-488; LYS-492; THR-548; ALA-562; LYS-606 AND ASN-617.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB071996 mRNA. Translation: BAC06350.1. AB071997 mRNA. Translation: BAC06351.1. AL833472 mRNA. Translation: CAI46114.1. Frameshift. BC028587 mRNA. Translation: AAH28587.1. AK027148 mRNA. Translation: BAB15672.1. Different initiation.
IPI	IPI00398836. IPI00745302. IPI00745916.
RefSeq	NP_001139475.1. NP_079076.3. NP_665917.1.
UniGene	Hs.657336
3D structure databases
HSSP	HSSP built from PDB template 1Q99 based on UniProtKB Q03656.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NG66. 8 interactions.
STRING	Q8NG66.
Proteomic databases
PRIDE	Q8NG66.
Genome annotation databases
Ensembl	ENST00000264996; ENSP00000264996; ENSG00000114670; Homo sapiens. [Genome view] ENST00000356918; ENSP00000349389; ENSG00000114670; Homo sapiens. [Genome view] ENST00000383366; ENSP00000372857; ENSG00000114670; Homo sapiens. [Genome view] ENST00000412440; ENSP00000411888; ENSG00000114670; Homo sapiens. [Genome view] ENST00000429253; ENSP00000397180; ENSG00000114670; Homo sapiens. [Genome view]
GeneID	79858.
KEGG	hsa:79858.
UCSC	uc003enx.1. human. uc003eny.1. human.
Organism-specific databases
GeneCards	GC03P132228.
H-InvDB	HIX0003682.
HGNC	HGNC:18593. NEK11.
HPA	HPA016908.
MIM	609779. gene.
PharmGKB	PA38595.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8NG66.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Gene expression databases
ArrayExpress	Q8NG66.
Bgee	Q8NG66.
CleanEx	HS_NEK11.
Genevestigator	Q8NG66.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	69585.
SOURCE	Search...

Entry information

Entry name

NEK11_HUMAN

Accession

Primary (citable) accession number: Q8NG66
Secondary accession number(s): Q5JPC0 , Q8NG65, Q8TBY1, Q9H5F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 1, 2002
Last modified:	October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.