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Q8NG08 (HELB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA helicase B

Short name=hDHB
EC=3.6.4.12
Gene names
Name:HELB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1087 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress. Ref.1 Ref.7

Catalytic activity

ATP + H2O = ADP + phosphate.

Enzyme regulation

Inhibited by salt concentration greater than 100 mM. Uses either magnesium or manganese ions to support helicase activity. Binds strongly to single-stranded DNA in the absence of ATP but dissociates readily in the presence of 1 mM ATP. Ref.1

Subunit structure

Binds to RPA1; this interaction promotes HELB recruitment to chromatin following DNA damage. Interacts with at least two subunits of pol-prim. Ref.1 Ref.7

Subcellular location

Note: Preferentially during S phase, recruited to chromatin following DNA damage induced by UV irradiation, or camptothecin or hydroxyurea treatment.

Tissue specificity

Highly expressed in testis and thymus and weakly in liver, spleen, kidney and brain. Ref.1

Sequence caution

The sequence BAB15754.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NG08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NG08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     562-574: NYSFYSWTQTMMT → TLDSYPVLNLVTC
     575-1087: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10871087DNA helicase B
PRO_0000338992

Amino acid modifications

Modified residue9671Phosphoserine By similarity
Modified residue9711Phosphoserine By similarity
Modified residue10271Phosphothreonine Ref.5

Natural variations

Alternative sequence562 – 57413NYSFY…QTMMT → TLDSYPVLNLVTC in isoform 2.
VSP_034086
Alternative sequence575 – 1087513Missing in isoform 2.
VSP_034087
Natural variant1721E → K.
Corresponds to variant rs35605829 [ dbSNP | Ensembl ].
VAR_043855
Natural variant1911L → P. Ref.2
Corresponds to variant rs4430553 [ dbSNP | Ensembl ].
VAR_043856
Natural variant2671L → F.
Corresponds to variant rs35138454 [ dbSNP | Ensembl ].
VAR_043857
Natural variant5751T → A.
Corresponds to variant rs58589183 [ dbSNP | Ensembl ].
VAR_061665
Natural variant9661P → L. Ref.2
Corresponds to variant rs1185244 [ dbSNP | Ensembl ].
VAR_043858
Natural variant9801T → I. Ref.2
Corresponds to variant rs1168312 [ dbSNP | Ensembl ].
VAR_043859

Experimental info

Mutagenesis4811K → A: No ATPase activity. Ref.1
Mutagenesis4991E → A: Loss of RPA1-binding; when associated with A-506 and A-510. Ref.7
Mutagenesis5061D → A: Loss of RPA1-binding; when associated with A-499 and A-510. Ref.7
Mutagenesis5101D → A: Loss of RPA1-binding; when associated with A-499 and A-506. Ref.7
Mutagenesis5911E → Q: No ATPase activity. Ref.1
Sequence conflict3661S → P in BAF83583. Ref.2
Sequence conflict8491F → S in BAF83583. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 05220F3186CE722B

FASTA1,087123,252
        10         20         30         40         50         60 
MARSSPYLRQ LQGPLLPPRD LVEEDDDYLN DDVEEDEESV FIDAEELCSG GVKAGSLPGC 

        70         80         90        100        110        120 
LRVSICDENT QETCKVFGRF PITGAWWRVK VQVKPVVGSR SYQYQVQGFP SYFLQSDMSP 

       130        140        150        160        170        180 
PNQKHICALF LKECEVSSDD VNKFLTWVKE VSNYKNLNFE NLRETLRTFH KETGRKDQKQ 

       190        200        210        220        230        240 
PTQNGQEELF LDNEMSLPLE NTIPFRNVMT ALQFPKIMEF LPVLLPRHFK WIIGSGSKEM 

       250        260        270        280        290        300 
LKEIEEILGT HPWKLGFSKI TYREWKLLRC EASWIAFCQC ESLLQLMTDL EKNALIMYSR 

       310        320        330        340        350        360 
LKQICREDGH TYVEVNDLTL TLSNHMSFHA ASESLKFLKD IGVVTYEKSC VFPYDLYHAE 

       370        380        390        400        410        420 
RAIAFSICDL MKKPPWHLCV DVEKVLASIH TTKPENSSDD ALNESKPDEV RLENPVDVVD 

       430        440        450        460        470        480 
TQDNGDHIWT NGENEINAEI SEVQLDQDQV EVPLDRDQVA ALEMICSNPV TVISGKGGCG 

       490        500        510        520        530        540 
KTTIVSRLFK HIEQLEEREV KKACEDFEQD QNASEEWITF TEQSQLEADK AIEVLLTAPT 

       550        560        570        580        590        600 
GKAAGLLRQK TGLHAYTLCQ VNYSFYSWTQ TMMTTNKPWK FSSVRVLVVD EGSLVSVGIF 

       610        620        630        640        650        660 
KSVLNLLCEH SKLSKLIILG DIRQLPSIEP GNLLKDLFET LKSRNCAIEL KTNHRAESQL 

       670        680        690        700        710        720 
IVDNATRISR RQFPKFDAEL NISDNPTLPI SIQDKTFIFV RLPEEDASSQ SSKTNHHSCL 

       730        740        750        760        770        780 
YSAVKTLLQE NNLQNAKTSQ FIAFRRQDCD LINDCCCKHY TGHLTKDHQS RLVFGIGDKI 

       790        800        810        820        830        840 
CCTRNAYLSD LLPENISGSQ QNNDLDASSE DFSGTLPDFA KNKRDFESNV RLCNGEIFFI 

       850        860        870        880        890        900 
TNDVTDVTFG KRRSLTINNM AGLEVTVDFK KLMKYCRIKH AWARTIHTFQ GSEEQTVVYV 

       910        920        930        940        950        960 
VGKAGRQHWQ HVYTAVTRGR CRVYVIAEES QLRNAIMKNS FPRKTRLKHF LQSKLSSSGA 

       970        980        990       1000       1010       1020 
PPADFPSPRK SSGDSGGPST PSASPLPVVT DHAMTNDVTW SEASSPDERT LTFAERWQLS 

      1030       1040       1050       1060       1070       1080 
SPDGVDTDDD LPKSRASKRT CGVNDDESPS KIFMVGESPQ VSSRLQNLRL NNLIPRQLFK 


PTDNQET 

« Hide

Isoform 2 [UniParc].

Checksum: 5628E8F75508B446
Show »

FASTA57465,443

References

« Hide 'large scale' references
[1]"A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication."
Taneja P., Gu J., Peng R., Carrick R., Uchiumi F., Ott R.D., Gustafson E., Podust V.N., Fanning E.
J. Biol. Chem. 277:40853-40861(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, ENZYME REGULATION, INTERACTION WITH RPA1 AND POL-PRIM COMPLEX, MUTAGENESIS OF LYS-481 AND GLU-591.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS PRO-191; LEU-966 AND ILE-980.
Tissue: Spleen.
[3]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[4]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1027, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[6]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress."
Guler G.D., Liu H., Vaithiyalingam S., Arnett D.R., Kremmer E., Chazin W.J., Fanning E.
J. Biol. Chem. 287:6469-6481(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RPA1, MUTAGENESIS OF GLU-499; ASP-506 AND ASP-510.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF319995 mRNA. Translation: AAM73554.1.
AK024464 mRNA. Translation: BAB15754.1. Different initiation.
AK290894 mRNA. Translation: BAF83583.1.
CCDSCCDS8976.1. [Q8NG08-1]
RefSeqNP_387467.2. NM_033647.3. [Q8NG08-1]
XP_005269291.1. XM_005269234.1. [Q8NG08-1]
UniGeneHs.505941.

3D structure databases

ProteinModelPortalQ8NG08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124978. 4 interactions.
IntActQ8NG08. 1 interaction.
STRING9606.ENSP00000247815.

PTM databases

PhosphoSiteQ8NG08.

Polymorphism databases

DMDM190359604.

Proteomic databases

MaxQBQ8NG08.
PaxDbQ8NG08.
PRIDEQ8NG08.

Protocols and materials databases

DNASU92797.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000247815; ENSP00000247815; ENSG00000127311. [Q8NG08-1]
ENST00000440906; ENSP00000396955; ENSG00000127311. [Q8NG08-2]
ENST00000545134; ENSP00000443287; ENSG00000127311. [Q8NG08-1]
GeneID92797.
KEGGhsa:92797.
UCSCuc001sti.3. human. [Q8NG08-1]

Organism-specific databases

CTD92797.
GeneCardsGC12P066696.
H-InvDBHIX0036722.
HGNCHGNC:17196. HELB.
MIM614539. gene.
neXtProtNX_Q8NG08.
PharmGKBPA134987279.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0507.
HOGENOMHOG000043085.
HOVERGENHBG097647.
InParanoidQ8NG08.
KOK15254.
OMAGRQHWQH.
OrthoDBEOG7GBG16.
PhylomeDBQ8NG08.
TreeFamTF336223.

Gene expression databases

ArrayExpressQ8NG08.
BgeeQ8NG08.
CleanExHS_HELB.
GenevestigatorQ8NG08.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027044. Helb.
IPR027417. P-loop_NTPase.
IPR027785. UvrD_like_helicase_C.
[Graphical view]
PANTHERPTHR18934:SF97. PTHR18934:SF97. 1 hit.
PfamPF13538. UvrD_C_2. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 6 hits.
ProtoNetSearch...

Other

GenomeRNAi92797.
NextBio77879.
PROQ8NG08.
SOURCESearch...

Entry information

Entry nameHELB_HUMAN
AccessionPrimary (citable) accession number: Q8NG08
Secondary accession number(s): A8K4C9, Q4G0T2, Q9H7L5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM