Reviewed, UniProtKB/Swiss-Prot Q8NFZ5 (TNIP2_HUMAN)

Names and origin

Protein names

Recommended name:
    TNFAIP3-interacting protein 2
Alternative name(s):
    Fetal liver LKB1-interacting protein
    A20-binding inhibitor of NF-kappa-B activation 2
      Short name=ABIN-2

Gene names

Name:	TNIP2
Synonyms:	ABIN2, FLIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Ref.2 Ref.6 Ref.7
Subunit structure	Interacts with STK11, TNFAIP3, IKBKG, NFKB1, MAP3K8 and RIPK1. Ref.2 Ref.6 Ref.7
Subcellular location	Cytoplasm. Ref.2
Tissue specificity	Ubiquitously expressed in all tissues examined. Ref.2

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.2 Ref.6 Ref.7 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IKBKG	Q9Y6K9	2	EBI-359372,EBI-81279
MAP3K8	P41279	3	EBI-359372,EBI-354900
NFKB1	P19838-1	4	EBI-359372,EBI-1452239
STK11	Q15831	4	EBI-359372,EBI-306838
TNFAIP3	P21580	1	EBI-359372,EBI-527670

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 Ref.5 Ref.1 (identifier: Q8NFZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q8NFZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	TNFAIP3-interacting protein 2		PRO_0000322583
Regions
	Coiled coil	29 – 117	89	Potential
	Coiled coil	196 – 226	31	Potential
	Coiled coil	255 – 340	86	Potential
Amino acid modifications
	Modified residue	186	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	1 – 107	107	Missing in isoform 2. Ref.3		VSP_052701
	Natural variant	396	1	A → V: dbSNP rs2269495. Ref.3 Ref.5		VAR_039463
Experimental info
	Sequence conflict	153	1	Q → H in CAC34835. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0F6B049C2B3483DC	FASTA	429	48,700
	10 20 30 40 50 60 MSRDPGSGGW EEAPRAAAAL CTLYHEAGQR LRRLQDQLAA RDALIARLRA RLAALEGDAA 70 80 90 100 110 120 PSLVDALLEQ VARFREQLRR QEGGAAEAQM RQEIERLTER LEEKEREMQQ LLSQPQHERE 130 140 150 160 170 180 KEVVLLRRSM AEGERARAAS DVLCRSLANE THQLRRTLTA TAHMCQHLAK CLDERQHAQR 190 200 210 220 230 240 NVGERSPDQS EHTDGHTSVQ SVIEKLQEEN RLLKQKVTHV EDLNAKWQRY NASRDEYVRG 250 260 270 280 290 300 LHAQLRGLQI PHEPELMRKE ISRLNRQLEE KINDCAEVKQ ELAASRTARD AALERVQMLE 310 320 330 340 350 360 QQILAYKDDF MSERADRERA QSRIQELEEK VASLLHQVSW RQDSREPDAG RIHAGSKTAK 370 380 390 400 410 420 YLAADALELM VPGGWRPGTG SQQPEPPAEG GHPGAAQRGQ GDLQCPHCLQ CFSDEQGEEL LRHVAECCQ
	Isoform 2. Checksum: 4B145A09A85133BE	FASTA	322	36,682
	10 20 30 40 50 60 MQQLLSQPQH EREKEVVLLR RSMAEGERAR AASDVLCRSL ANETHQLRRT LTATAHMCQH 70 80 90 100 110 120 LAKCLDERQH AQRNVGERSP DQSEHTDGHT SVQSVIEKLQ EENRLLKQKV THVEDLNAKW 130 140 150 160 170 180 QRYNASRDEY VRGLHAQLRG LQIPHEPELM RKEISRLNRQ LEEKINDCAE VKQELAASRT 190 200 210 220 230 240 ARDAALERVQ MLEQQILAYK DDFMSERADR ERAQSRIQEL EEKVASLLHQ VSWRQDSREP 250 260 270 280 290 300 DAGRIHAGSK TAKYLAADAL ELMVPGGWRP GTGSQQPEPP AEGGHPGAAQ RGQGDLQCPH 310 320 CLQCFSDEQG EELLRHVAEC CQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel A20-binding inhibitor of nuclear factor-kappaB activation termed ABIN-2." Van Huffel S.C., Delaei F., Heyninck K., De Valck D., Beyaert R. J. Biol. Chem. 276:30216-30223(2001) [PubMed: 11390377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"An LKB1-interacting protein negatively regulates TNFalpha-induced NF-kappaB activation." Liu W.-K., Chien C.-Y., Chou C.-K., Su J.-Y. J. Biomed. Sci. 10:242-252(2003) [PubMed: 12595760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH STK11.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-396. Tissue: Kidney epithelium.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-396. Tissue: Uterus.
[6]	"The inhibitor ABIN-2 disrupts the interaction of receptor-interacting protein with the kinase subunit IKKgamma to block activation of the transcription factor NF-kappaB and potentiate apoptosis." Liu W.-K., Yen P.-F., Chien C.-Y., Fann M.-J., Su J.-Y., Chou C.-K. Biochem. J. 378:867-876(2004) [PubMed: 14653779] [Abstract] Cited for: FUNCTION, INTERACTION WITH IKBKG.
[7]	"ABIN-2 forms a ternary complex with TPL-2 and NF-kappa B1 p105 and is essential for TPL-2 protein stability." Lang V., Symons A., Watton S.J., Janzen J., Soneji Y., Beinke S., Howell S., Ley S.C. Mol. Cell. Biol. 24:5235-5248(2004) [PubMed: 15169888] [Abstract] Cited for: FUNCTION, INTERACTION WITH NFKB1; MAP3K8 AND TNFAIP3.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ304866 mRNA. Translation: CAC34835.1. AF372839 mRNA. Translation: AAM21315.1. AK026176 mRNA. Translation: BAB15382.1. AK096296 mRNA. Translation: BAG53250.1. CH471131 Genomic DNA. Translation: EAW82514.1. BC002740 mRNA. Translation: AAH02740.2.
IPI	IPI00168953. IPI00884948.
RefSeq	NP_077285.2.
UniGene	Hs.368551
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NFZ5. 9 interactions.
Proteomic databases
PRIDE	Q8NFZ5.
Genome annotation databases
Ensembl	ENSG00000168884. Homo sapiens. [Contig view]
GeneID	79155.
KEGG	hsa:79155.
Organism-specific databases
GeneCards	GC04M002780.
HGNC	HGNC:19118. TNIP2.
MIM	610669. gene.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8NFZ5.
OMA	Q8NFZ5. ASDVLCR.
Gene expression databases
ArrayExpress	Q8NFZ5.
Bgee	Q8NFZ5.
CleanEx	HS_TNIP2.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	68079.
SOURCE	Search...

Entry information

Entry name

TNIP2_HUMAN

Accession

Primary (citable) accession number: Q8NFZ5
Secondary accession number(s): B3KTY8 , Q7L5L2, Q9BQR6, Q9H682

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)