Q8NFZ5 (TNIP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TNFAIP3-interacting protein 2

Alternative name(s):
A20-binding inhibitor of NF-kappa-B activation 2
Short name=ABIN-2
Fetal liver LKB1-interacting protein

Gene names

Name:	TNIP2
Synonyms:	ABIN2, FLIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Ref.2 Ref.6 Ref.8 Ref.9
Subunit structure	Interacts with STK11/LKB1, TNFAIP3, IKBKG, NFKB1, MAP3K8, TEK and RIPK1. Ref.2 Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm Ref.2.
Tissue specificity	Ubiquitously expressed in all tissues examined. Ref.2

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction Ref.2Ref.8Ref.9. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IKBKG	Q9Y6K9	6	EBI-359372,EBI-81279
MAP3K8	P41279	8	EBI-359372,EBI-354900
NFKB1	P19838-1	8	EBI-359372,EBI-1452239
STK11	Q15831	4	EBI-359372,EBI-306838

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.2 Ref.6 (identifier: Q8NFZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q8NFZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	TNFAIP3-interacting protein 2		PRO_0000322583
Regions
	Coiled coil	29 – 117	89	Potential
	Coiled coil	196 – 226	31	Potential
	Coiled coil	255 – 340	86	Potential
Amino acid modifications
	Modified residue	186	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	1 – 107	107	Missing in isoform 2. Ref.3		VSP_052701
	Natural variant	396	1	A → V. Ref.3 Ref.6 Corresponds to variant rs2269495 [ dbSNP | Ensembl ].		VAR_039463
Experimental info
	Sequence conflict	153	1	Q → H in CAC34835. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0F6B049C2B3483DC	FASTA	429	48,700
	10 20 30 40 50 60 MSRDPGSGGW EEAPRAAAAL CTLYHEAGQR LRRLQDQLAA RDALIARLRA RLAALEGDAA 70 80 90 100 110 120 PSLVDALLEQ VARFREQLRR QEGGAAEAQM RQEIERLTER LEEKEREMQQ LLSQPQHERE 130 140 150 160 170 180 KEVVLLRRSM AEGERARAAS DVLCRSLANE THQLRRTLTA TAHMCQHLAK CLDERQHAQR 190 200 210 220 230 240 NVGERSPDQS EHTDGHTSVQ SVIEKLQEEN RLLKQKVTHV EDLNAKWQRY NASRDEYVRG 250 260 270 280 290 300 LHAQLRGLQI PHEPELMRKE ISRLNRQLEE KINDCAEVKQ ELAASRTARD AALERVQMLE 310 320 330 340 350 360 QQILAYKDDF MSERADRERA QSRIQELEEK VASLLHQVSW RQDSREPDAG RIHAGSKTAK 370 380 390 400 410 420 YLAADALELM VPGGWRPGTG SQQPEPPAEG GHPGAAQRGQ GDLQCPHCLQ CFSDEQGEEL LRHVAECCQ
	Isoform 2 [UniParc]. Checksum: 4B145A09A85133BE	FASTA	322	36,682
	10 20 30 40 50 60 MQQLLSQPQH EREKEVVLLR RSMAEGERAR AASDVLCRSL ANETHQLRRT LTATAHMCQH 70 80 90 100 110 120 LAKCLDERQH AQRNVGERSP DQSEHTDGHT SVQSVIEKLQ EENRLLKQKV THVEDLNAKW 130 140 150 160 170 180 QRYNASRDEY VRGLHAQLRG LQIPHEPELM RKEISRLNRQ LEEKINDCAE VKQELAASRT 190 200 210 220 230 240 ARDAALERVQ MLEQQILAYK DDFMSERADR ERAQSRIQEL EEKVASLLHQ VSWRQDSREP 250 260 270 280 290 300 DAGRIHAGSK TAKYLAADAL ELMVPGGWRP GTGSQQPEPP AEGGHPGAAQ RGQGDLQCPH 310 320 CLQCFSDEQG EELLRHVAEC CQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel A20-binding inhibitor of nuclear factor-kappaB activation termed ABIN-2." Van Huffel S.C., Delaei F., Heyninck K., De Valck D., Beyaert R. J. Biol. Chem. 276:30216-30223(2001) [PubMed: 11390377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"An LKB1-interacting protein negatively regulates TNFalpha-induced NF-kappaB activation." Liu W.-K., Chien C.-Y., Chou C.-K., Su J.-Y. J. Biomed. Sci. 10:242-252(2003) [PubMed: 12595760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH STK11/LKB1.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-396. Tissue: Kidney epithelium.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-396. Tissue: Uterus.
[7]	"The antiinflammatory endothelial tyrosine kinase Tie2 interacts with a novel nuclear factor-kappaB inhibitor ABIN-2." Hughes D.P., Marron M.B., Brindle N.P. Circ. Res. 92:630-636(2003) [PubMed: 12609966] [Abstract] Cited for: INTERACTION WITH TEK.
[8]	"The inhibitor ABIN-2 disrupts the interaction of receptor-interacting protein with the kinase subunit IKKgamma to block activation of the transcription factor NF-kappaB and potentiate apoptosis." Liu W.-K., Yen P.-F., Chien C.-Y., Fann M.-J., Su J.-Y., Chou C.-K. Biochem. J. 378:867-876(2004) [PubMed: 14653779] [Abstract] Cited for: FUNCTION, INTERACTION WITH IKBKG.
[9]	"ABIN-2 forms a ternary complex with TPL-2 and NF-kappa B1 p105 and is essential for TPL-2 protein stability." Lang V., Symons A., Watton S.J., Janzen J., Soneji Y., Beinke S., Howell S., Ley S.C. Mol. Cell. Biol. 24:5235-5248(2004) [PubMed: 15169888] [Abstract] Cited for: FUNCTION, INTERACTION WITH NFKB1; MAP3K8 AND TNFAIP3.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ304866 mRNA. Translation: CAC34835.1. AF372839 mRNA. Translation: AAM21315.1. AK026176 mRNA. Translation: BAB15382.1. AK096296 mRNA. Translation: BAG53250.1. CH471131 Genomic DNA. Translation: EAW82514.1. AL121750, AL110117 Genomic DNA. Translation: CAM28233.1. AL110117, AL121750 Genomic DNA. Translation: CAM28252.1. CH471131 Genomic DNA. Translation: EAW82516.1. BC002740 mRNA. Translation: AAH02740.2.
IPI	IPI00168953. IPI00884948.
RefSeq	NP_001154999.1. NM_001161527.1. NP_077285.3. NM_024309.3.
UniGene	Hs.726088.
3D structure databases
ProteinModelPortal	Q8NFZ5.
SMR	Q8NFZ5. Positions 258-342, 400-429.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27617N.
IntAct	Q8NFZ5. 13 interactions.
MINT	MINT-97349.
STRING	Q8NFZ5.
PTM databases
PhosphoSite	Q8NFZ5.
Polymorphism databases
DMDM	74715616.
Proteomic databases
PRIDE	Q8NFZ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000315423; ENSP00000321203; ENSG00000168884.
GeneID	79155.
KEGG	hsa:79155.
UCSC	uc003gff.1. human.
Organism-specific databases
CTD	79155.
GeneCards	GC04M002780.
H-InvDB	HIX0004036.
HGNC	HGNC:19118. TNIP2.
HPA	CAB020818.
MIM	610669. gene.
neXtProt	NX_Q8NFZ5.
PharmGKB	PA134957006.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07304.
GeneTree	ENSGT00390000009019.
HOGENOM	HBG445040.
HOVERGEN	HBG055553.
InParanoid	Q8NFZ5.
OMA	ASDILCR.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q8NFZ5.
Bgee	Q8NFZ5.
CleanEx	HS_TNIP2.
Genevestigator	Q8NFZ5.
Family and domain databases
InterPro	IPR022008. EABR. [Graphical view]
Pfam	PF12180. EABR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	68079.
SOURCE	Search...

Entry information

Entry name

TNIP2_HUMAN

Accession

Primary (citable) accession number: Q8NFZ5
Secondary accession number(s): B1AKS4 , B3KTY8, D3DVQ9, Q7L5L2, Q9BQR6, Q9H682

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.