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Q8NFZ4 (NLGN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroligin-2
Gene names
Name:NLGN2
Synonyms:KIAA1366
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion By similarity.

Subunit structure

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with INADL. Interacts with GPHN By similarity. Interacts with MDGA1 and MDGA2 By similarity. Ref.3

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity. Note: Detected at postsynaptic membranes in brain. Detected at dendritic spines in cultured neurons. Colocalizes with GPHN and ARHGEF9 at neuronal cell membranes By similarity. Localized at presynaptic membranes in retina. Colocalizes with GABRG2 at inhibitory synapses in the retina By similarity.

Tissue specificity

Expressed in the blood vessel walls. Detected in colon, brain and pancreas islets of Langerhans (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells. Ref.4 Ref.5

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell-cell adhesion

Non-traceable author statement PubMed 10892652. Source: UniProtKB

cell-cell junction maintenance

Non-traceable author statement PubMed 10892652. Source: UniProtKB

gephyrin clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

locomotory exploration behavior

Inferred from electronic annotation. Source: Ensembl

neuromuscular process controlling balance

Inferred from electronic annotation. Source: Ensembl

neuron cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of excitatory postsynaptic membrane potential

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of inhibitory postsynaptic membrane potential

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synapse assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic transmission, GABAergic

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic transmission, glutamatergic

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic density protein 95 clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

presynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

protein localization to synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of respiratory gaseous exchange by neurological system process

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of synaptic transmission

Inferred from sequence or structural similarity. Source: BHF-UCL

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

synapse assembly

Non-traceable author statement PubMed 10892652. Source: UniProtKB

synapse organization

Inferred from sequence or structural similarity. Source: BHF-UCL

terminal button organization

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from sequence or structural similarity. Source: BHF-UCL

inhibitory synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

membrane

Non-traceable author statement PubMed 10892652. Source: UniProtKB

postsynaptic membrane

Non-traceable author statement PubMed 10892652. Source: UniProtKB

presynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular_functioncell adhesion molecule binding

Inferred from sequence or structural similarity. Source: BHF-UCL

neurexin family protein binding

Non-traceable author statement PubMed 10892652. Source: UniProtKB

receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 835821Neuroligin-2
PRO_0000008643

Regions

Topological domain15 – 677663Extracellular Potential
Transmembrane678 – 69821Helical; Potential
Topological domain699 – 835137Cytoplasmic Potential

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1361N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Disulfide bond106 ↔ 141 By similarity
Disulfide bond317 ↔ 328 By similarity
Disulfide bond487 ↔ 521 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8NFZ4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 359938630193EF87

FASTA83590,820
        10         20         30         40         50         60 
MWLLALCLVG LAGAQRGGGG PGGGAPGGPG LGLGSLGEER FPVVNTAYGR VRGVRRELNN 

        70         80         90        100        110        120 
EILGPVVQFL GVPYATPPLG ARRFQPPEAP ASWPGVRNAT TLPPACPQNL HGALPAIMLP 

       130        140        150        160        170        180 
VWFTDNLEAA ATYVQNQSED CLYLNLYVPT EDGPLTKKRD EATLNPPDTD IRDPGKKPVM 

       190        200        210        220        230        240 
LFLHGGSYME GTGNMFDGSV LAAYGNVIVA TLNYRLGVLG FLSTGDQAAK GNYGLLDQIQ 

       250        260        270        280        290        300 
ALRWLSENIA HFGGDPERIT IFGSGAGASC VNLLILSHHS EGLFQKAIAQ SGTAISSWSV 

       310        320        330        340        350        360 
NYQPLKYTRL LAAKVGCDRE DSAEAVECLR RKPSRELVDQ DVQPARYHIA FGPVVDGDVV 

       370        380        390        400        410        420 
PDDPEILMQQ GEFLNYDMLI GVNQGEGLKF VEDSAESEDG VSASAFDFTV SNFVDNLYGY 

       430        440        450        460        470        480 
PEGKDVLRET IKFMYTDWAD RDNGEMRRKT LLALFTDHQW VAPAVATAKL HADYQSPVYF 

       490        500        510        520        530        540 
YTFYHHCQAE GRPEWADAAH GDELPYVFGV PMVGATDLFP CNFSKNDVML SAVVMTYWTN 

       550        560        570        580        590        600 
FAKTGDPNQP VPQDTKFIHT KPNRFEEVVW SKFNSKEKQY LHIGLKPRVR DNYRANKVAF 

       610        620        630        640        650        660 
WLELVPHLHN LHTELFTTTT RLPPYATRWP PRPPAGAPGT RRPPPPATLP PEPEPEPGPR 

       670        680        690        700        710        720 
AYDRFPGDSR DYSTELSVTV AVGASLLFLN ILAFAALYYK RDRRQELRCR RLSPPGGSGS 

       730        740        750        760        770        780 
GVPGGGPLLP AAGRELPPEE ELVSLQLKRG GGVGADPAEA LRPACPPDYT LALRRAPDDV 

       790        800        810        820        830 
PLLAPGALTL LPSGLGPPPP PPPPSLHPFG PFPPPPPTAT SHNNTLPHPH STTRV 

« Hide

References

« Hide 'large scale' references
[1]"Mutations of the X-linked genes encoding neuroligins NLGN3 and NLGN4 are associated with autism."
Jamain S., Quach H., Betancur C., Rastam M., Colineaux C., Gillberg I.C., Soderstrom H., Giros B., Leboyer M., Gillberg C., Bourgeron T., Nyden A., Philippe A., Cohen D., Chabane N., Mouren-Simeoni M.C., Brice A., Sponheim E. expand/collapse author list , Spurkland I., Skjeldal O.H., Coleman M., Pearl P.L., Cohen I.L., Tsiouris J., Zappella M., Menchetti G., Pompella A., Aschauer H., Van Maldergem L.
Nat. Genet. 34:27-29(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-835.
Tissue: Brain.
[3]"Binding of neuroligins to PSD-95."
Irie M., Hata Y., Takeuchi M., Ichtchenko K., Toyoda A., Hirao K., Takai Y., Rosahl T.W., Suedhof T.C.
Science 277:1511-1515(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG4.
[4]"Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion."
Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D.
Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[5]"The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions."
Bottos A., Destro E., Rissone A., Graziano S., Cordara G., Assenzio B., Cera M.R., Mascia L., Bussolino F., Arese M.
Proc. Natl. Acad. Sci. U.S.A. 106:20782-20787(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF376802 mRNA. Translation: AAM46111.1.
AB037787 mRNA. Translation: BAA92604.1.
RefSeqNP_065846.1. NM_020795.3.
UniGeneHs.26229.

3D structure databases

ProteinModelPortalQ8NFZ4.
SMRQ8NFZ4. Positions 42-609.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121611. 7 interactions.
STRING9606.ENSP00000305288.

Protein family/group databases

MEROPSS09.995.

PTM databases

PhosphoSiteQ8NFZ4.

Polymorphism databases

DMDM31076824.

Proteomic databases

PaxDbQ8NFZ4.
PRIDEQ8NFZ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302926; ENSP00000305288; ENSG00000169992.
ENST00000575301; ENSP00000461168; ENSG00000169992.
GeneID57555.
KEGGhsa:57555.
UCSCuc002ggt.1. human.

Organism-specific databases

CTD57555.
GeneCardsGC17P007311.
HGNCHGNC:14290. NLGN2.
HPAHPA055321.
MIM606479. gene.
neXtProtNX_Q8NFZ4.
PharmGKBPA31648.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000231424.
HOVERGENHBG008839.
InParanoidQ8NFZ4.
KOK07378.
OMADIRDPGK.
OrthoDBEOG7RBZ7R.
PhylomeDBQ8NFZ4.
TreeFamTF326187.

Gene expression databases

BgeeQ8NFZ4.
CleanExHS_NLGN2.
GenevestigatorQ8NFZ4.

Family and domain databases

InterProIPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Neuroligin.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR01090. NEUROLIGIN.
PROSITEPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNLGN2.
GenomeRNAi57555.
NextBio64034.
PROQ8NFZ4.
SOURCESearch...

Entry information

Entry nameNLGN2_HUMAN
AccessionPrimary (citable) accession number: Q8NFZ4
Secondary accession number(s): Q9P2I1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM