Q8NFZ0 (FBX18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box only protein 18
EC=3.6.4.12

Alternative name(s):
F-box DNA helicase 1

Gene names

Name:	FBXO18
Synonyms:	FBH1, FBX18

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent ATPase. Unwinds double-stranded DNA in a 3' to 5' direction. Ref.1 Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Ref.1
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Part of a SCF E3 ubiquitin ligase complex consisting of SKP1, CUL1, RBX1 and FBXO18. Interacts with SKP1. Ref.1
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the helicase family. UvrD subfamily. Contains 1 F-box domain.
Sequence caution	The sequence AAM73631.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB55073.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB55154.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NFZ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NFZ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSYEVTSGCHWTCQVPESCDNGLHCAGPLGHLHRRCQRTSAHLLVFTEHAEM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	F-box only protein 18		PRO_0000119901
Regions
	Domain	138 – 184	47	F-box
Natural variations
	Alternative sequence	1	1	M → MSYEVTSGCHWTCQVPESCD NGLHCAGPLGHLHRRCQRTS AHLLVFTEHAEM in isoform 2.		VSP_037942
Experimental info
	Sequence conflict	234	1	W → R in BAC04535. Ref.3
	Sequence conflict	339	1	Missing in AAP97700. Ref.2
	Sequence conflict	339	1	Missing in BAB55154. Ref.3
	Sequence conflict	425	1	K → N in AAP97705. Ref.2
	Sequence conflict	844	1	N → S in BAC04535. Ref.3
	Sequence conflict	984	1	P → L in AAP97700. Ref.2
	Sequence conflict	984	1	P → L in BAB55154. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: E20EB343E9D05C4D	FASTA	1,043	117,686
	10 20 30 40 50 60 MRRFKRKHLT AIDCQHLARS HLAVTQPFGQ RWTNRDPNHG LYPKPRTKRG SRGQGSQRCI 70 80 90 100 110 120 PEFFLAGKQP CTNDMAKSNS VGQDSCQDSE GDMIFPAESS CALPQEGSAG PGSPGSAPPS 130 140 150 160 170 180 RKRSWSSEEE SNQATGTSRW DGVSKKAPRH HLSVPCTRPR EARQEAEDST SRLSAESGET 190 200 210 220 230 240 DQDAGDVGPD PIPDSYYGLL GTLPCQEALS HICSLPSEVL RHVFAFLPVE DLYWNLSLVC 250 260 270 280 290 300 HLWREIISDP LFIPWKKLYH RYLMNEEQAV SKVDGILSNC GIEKESDLCV LNLIRYTATT 310 320 330 340 350 360 KCSPSVDPER VLWSLRDHPL LPEAEACVRQ HLPDLYAAAG GVNIWALVAA VVLLSSSVND 370 380 390 400 410 420 IQRLLFCLRR PSSTVTMPDV TETLYCIAVL LYAMREKGIN ISNRIHYNIF YCLYLQENSC 430 440 450 460 470 480 TQATKVKEEP SVWPGKKTIQ LTHEQQLILN HKMEPLQVVK IMAFAGTGKT STLVKYAEKW 490 500 510 520 530 540 SQSRFLYVTF NKSIAKQAER VFPSNVICKT FHSMAYGHIG RKYQSKKKLN LFKLTPFMVN 550 560 570 580 590 600 SVLAEGKGGF IRAKLVCKTL ENFFASADEE LTIDHVPIWC KNSQGQRVMV EQSEKLNGVL 610 620 630 640 650 660 EASRLWDNMR KLGECTEEAH QMTHDGYLKL WQLSKPSLAS FDAIFVDEAQ DCTPAIMNIV 670 680 690 700 710 720 LSQPCGKIFV GDPHQQIYTF RGAVNALFTV PHTHVFYLTQ SFRFGVEIAY VGATILDVCK 730 740 750 760 770 780 RVRKKTLVGG NHQSGIRGDA KGQVALLSRT NANVFDEAVR VTEGEFPSRI HLIGGIKSFG 790 800 810 820 830 840 LDRIIDIWIL LQPEEERRKQ NLVIKDKFIR RWVHKEGFSG FKRYVTAAED KELEAKIAVV 850 860 870 880 890 900 EKYNIRIPEL VQRIEKCHIE DLDFAEYILG TVHKAKGLEF DTVHVLDDFV KVPCARHNLP 910 920 930 940 950 960 QLPHFRVESF SEDEWNLLYV AVTRAKKRLI MTKSLENILT LAGEYFLQAE LTSNVLKTGV 970 980 990 1000 1010 1020 VRCCVGQCNN AIPVDTVLTM KKLPITYSNR KENKGGYLCH SCAEQRIGPL AFLTASPEQV 1030 1040 RAMERTVENI VLPRHEALLF LVF
	Isoform 2 [UniParc]. Checksum: F2A3ED56FBC690B4	FASTA	1,094	123,375
	10 20 30 40 50 60 MSYEVTSGCH WTCQVPESCD NGLHCAGPLG HLHRRCQRTS AHLLVFTEHA EMRRFKRKHL 70 80 90 100 110 120 TAIDCQHLAR SHLAVTQPFG QRWTNRDPNH GLYPKPRTKR GSRGQGSQRC IPEFFLAGKQ 130 140 150 160 170 180 PCTNDMAKSN SVGQDSCQDS EGDMIFPAES SCALPQEGSA GPGSPGSAPP SRKRSWSSEE 190 200 210 220 230 240 ESNQATGTSR WDGVSKKAPR HHLSVPCTRP REARQEAEDS TSRLSAESGE TDQDAGDVGP 250 260 270 280 290 300 DPIPDSYYGL LGTLPCQEAL SHICSLPSEV LRHVFAFLPV EDLYWNLSLV CHLWREIISD 310 320 330 340 350 360 PLFIPWKKLY HRYLMNEEQA VSKVDGILSN CGIEKESDLC VLNLIRYTAT TKCSPSVDPE 370 380 390 400 410 420 RVLWSLRDHP LLPEAEACVR QHLPDLYAAA GGVNIWALVA AVVLLSSSVN DIQRLLFCLR 430 440 450 460 470 480 RPSSTVTMPD VTETLYCIAV LLYAMREKGI NISNRIHYNI FYCLYLQENS CTQATKVKEE 490 500 510 520 530 540 PSVWPGKKTI QLTHEQQLIL NHKMEPLQVV KIMAFAGTGK TSTLVKYAEK WSQSRFLYVT 550 560 570 580 590 600 FNKSIAKQAE RVFPSNVICK TFHSMAYGHI GRKYQSKKKL NLFKLTPFMV NSVLAEGKGG 610 620 630 640 650 660 FIRAKLVCKT LENFFASADE ELTIDHVPIW CKNSQGQRVM VEQSEKLNGV LEASRLWDNM 670 680 690 700 710 720 RKLGECTEEA HQMTHDGYLK LWQLSKPSLA SFDAIFVDEA QDCTPAIMNI VLSQPCGKIF 730 740 750 760 770 780 VGDPHQQIYT FRGAVNALFT VPHTHVFYLT QSFRFGVEIA YVGATILDVC KRVRKKTLVG 790 800 810 820 830 840 GNHQSGIRGD AKGQVALLSR TNANVFDEAV RVTEGEFPSR IHLIGGIKSF GLDRIIDIWI 850 860 870 880 890 900 LLQPEEERRK QNLVIKDKFI RRWVHKEGFS GFKRYVTAAE DKELEAKIAV VEKYNIRIPE 910 920 930 940 950 960 LVQRIEKCHI EDLDFAEYIL GTVHKAKGLE FDTVHVLDDF VKVPCARHNL PQLPHFRVES 970 980 990 1000 1010 1020 FSEDEWNLLY VAVTRAKKRL IMTKSLENIL TLAGEYFLQA ELTSNVLKTG VVRCCVGQCN 1030 1040 1050 1060 1070 1080 NAIPVDTVLT MKKLPITYSN RKENKGGYLC HSCAEQRIGP LAFLTASPEQ VRAMERTVEN 1090 IVLPRHEALL FLVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel human DNA helicase hFBH1 is an F-box protein." Kim J., Kim J.-H., Lee S.-H., Kim D.-H., Kang H.-Y., Bae S.-H., Pan Z.-Q., Seo Y.-S. J. Biol. Chem. 277:24530-24537(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH SKP1, IDENTIFICATION IN SCF COMPLEX.
[2]	Zan Q., Guo J.H., Yu L. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Choriocarcinoma and Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland, Testis carcinoma and Tongue.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Pancreas and Testis.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 763-1043 (ISOFORMS 1/2). Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF380349 mRNA. Translation: AAM73631.1. Different initiation. AF454502 mRNA. Translation: AAP97700.1. AF456237 mRNA. Translation: AAP97705.1. AK027381 mRNA. Translation: BAB55073.1. Different initiation. AK027496 mRNA. Translation: BAB55154.1. Different initiation. AK095343 mRNA. Translation: BAC04535.1. AL137186 Genomic DNA. Translation: CAI41073.1. AL137186 Genomic DNA. Translation: CAI41074.1. CH471072 Genomic DNA. Translation: EAW86426.1. BC012762 mRNA. Translation: AAH12762.2. BC032674 mRNA. Translation: AAH32674.2. BC113377 mRNA. Translation: AAI13378.1. AL133069 mRNA. Translation: CAB61392.1.
IPI	IPI00375254. IPI00409721.
PIR	T42669.
RefSeq	NP_001245381.1. NM_001258452.1. NP_001245382.1. NM_001258453.1. NP_116196.3. NM_032807.4. NP_835363.1. NM_178150.2.
UniGene	Hs.498543.
3D structure databases
ProteinModelPortal	Q8NFZ0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NFZ0. 3 interactions.
PTM databases
PhosphoSite	Q8NFZ0.
Polymorphism databases
DMDM	45476952.
Proteomic databases
PaxDb	Q8NFZ0.
PRIDE	Q8NFZ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000362091; ENSP00000355415; ENSG00000134452. ENST00000379999; ENSP00000369335; ENSG00000134452.
GeneID	84893.
KEGG	hsa:84893.
UCSC	uc001iir.3. human. uc001iit.3. human.
Organism-specific databases
CTD	84893.
GeneCards	GC10P005931.
HGNC	HGNC:13620. FBXO18.
HPA	HPA002844.
MIM	607222. gene.
neXtProt	NX_Q8NFZ0.
PharmGKB	PA134948258.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0210.
HOVERGEN	HBG051568.
InParanoid	Q8NFZ0.
KO	K10300.
OMA	FPSNVIC.
OrthoDB	EOG4G7BZ4.
Gene expression databases
ArrayExpress	Q8NFZ0.
Bgee	Q8NFZ0.
Genevestigator	Q8NFZ0.
GermOnline	ENSG00000134452. Homo sapiens.
Family and domain databases
InterPro	IPR014017. DNA_helicase_UvrD-like_C. IPR000212. DNA_helicase_UvrD/REP. IPR001810. F-box_dom_cyclin-like. IPR014016. UvrD-like_ATP-bd. [Graphical view]
PANTHER	PTHR11070. PTHR11070. 1 hit.
Pfam	PF00580. UvrD-helicase. 1 hit. PF13361. UvrD_C. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FBXO18. human.
GenomeRNAi	84893.
NextBio	75230.
SOURCE	Search...

Entry information

Entry name

FBX18_HUMAN

Accession

Primary (citable) accession number: Q8NFZ0
Secondary accession number(s): Q5JVB0 , Q5JVB1, Q7Z4Q6, Q7Z4R0, Q8N1P5, Q8N586, Q96E82, Q96K67, Q96SW7, Q9UFB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.