Q8NFU5 (IPMK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol polyphosphate multikinase
EC=2.7.1.151

Alternative name(s):
Inositol 1,3,4,6-tetrakisphosphate 5-kinase

Gene names

Name:	IPMK
Synonyms:	IMPK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Ref.1 Ref.2
Catalytic activity	ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate. ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate.
Subcellular location	Nucleus Ref.1.
Tissue specificity	Ubiquitous, with the highest expression in skeletal muscle, liver, placenta, lung, peripheral blood leukocytes, kidney, spleen and colon. Ref.2
Sequence similarities	Belongs to the inositol phosphokinase (IPK) family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW inositol-1,4,5-trisphosphate 6-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Inositol polyphosphate multikinase		PRO_0000066870
Regions
	Region	140 – 148	9	Substrate binding By similarity
	Motif	320 – 330	11	Nuclear localization signal
Amino acid modifications
	Modified residue	7	1	Phosphoserine Ref.4 Ref.5
	Modified residue	21	1	Phosphothreonine Ref.5
	Modified residue	22	1	Phosphoserine Ref.5
	Modified residue	348	1	Phosphoserine Ref.5
	Modified residue	350	1	Phosphoserine Ref.5
Natural variations
	Natural variant	349	1	M → I. Corresponds to variant rs2275443 [ dbSNP | Ensembl ].		VAR_022112
Experimental info
	Mutagenesis	322 – 323	2	RK → QQ: Interferes with nuclear localization. Ref.1
	Mutagenesis	327 – 328	2	KK → QQ: Interferes with nuclear localization. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8NFU5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 622D678696A892F9	FASTA	416	47,222
	10 20 30 40 50 60 MATEPPSPLR VEAPGPPEMR TSPAIESTPE GTPQPAGGRL RFLNGCVPLS HQVAGHMYGK 70 80 90 100 110 120 DKVGILQHPD GTVLKQLQPP PRGPRELEFY NMVYAADCFD GVLLELRKYL PKYYGIWSPP 130 140 150 160 170 180 TAPNDLYLKL EDVTHKFNKP CIMDVKIGQK SYDPFASSEK IQQQVSKYPL MEEIGFLVLG 190 200 210 220 230 240 MRVYHVHSDS YETENQHYGR SLTKETIKDG VSRFFHNGYC LRKDAVAASI QKIEKILQWF 250 260 270 280 290 300 ENQKQLNFYA SSLLFVYEGS SQPTTTKLND RTLAEKFLSK GQLSDTEVLE YNNNFHVLSS 310 320 330 340 350 360 TANGKIESSV GKSLSKMYAR HRKIYTKKHH SQTSLKVENL EQDNGWKSMS QEHLNGNVLS 370 380 390 400 410 QLEKVFYHLP TGCQEIAEVE VRMIDFAHVF PSNTIDEGYV YGLKHLISVL RSILDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization." Nalaskowski M.M., Deschermeier C., Fanick W., Mayr G.W. Biochem. J. 366:549-556(2002) [PubMed: 12027805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF 322-ARG-LYS-323 AND 327-LYS-LYS-328, SUBCELLULAR LOCATION.
[2]	"The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase." Chang S.-C., Miller A.L., Feng Y., Wente S.R., Majerus P.W. J. Biol. Chem. 277:43836-43843(2002) [PubMed: 12223481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; THR-21; SER-22; SER-348 AND SER-350, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF432853 mRNA. Translation: AAM97838.1. BK000580 mRNA. Translation: DAA01362.1. BC065709 mRNA. Translation: AAH65709.1.
IPI	IPI00168907.
RefSeq	NP_689416.1. NM_152230.4.
UniGene	Hs.30280.
3D structure databases
ProteinModelPortal	Q8NFU5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NFU5.
PTM databases
PhosphoSite	Q8NFU5.
Polymorphism databases
DMDM	50401072.
Proteomic databases
PRIDE	Q8NFU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373935; ENSP00000363046; ENSG00000151151.
GeneID	253430.
KEGG	hsa:253430.
UCSC	uc001jkb.1. human.
Organism-specific databases
CTD	253430.
GeneCards	GC10M059951.
H-InvDB	HIX0008839.
HGNC	HGNC:20739. IPMK.
HPA	HPA037837.
MIM	609851. gene.
neXtProt	NX_Q8NFU5.
PharmGKB	PA134903369.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08133.
GeneTree	ENSGT00390000014381.
HOGENOM	HBG564560.
HOVERGEN	HBG052124.
InParanoid	Q8NFU5.
OMA	EHLNGNV.
OrthoDB	EOG4NVZKR.
PhylomeDB	Q8NFU5.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000151151-MONOMER.
BRENDA	2.7.1.151. 2681.
Gene expression databases
ArrayExpress	Q8NFU5.
Bgee	Q8NFU5.
CleanEx	HS_IPMK.
Genevestigator	Q8NFU5.
GermOnline	ENSG00000151151. Homo sapiens.
Family and domain databases
InterPro	IPR005522. IPK. [Graphical view]
KO	K00328.
PANTHER	PTHR12400. IPK. 1 hit.
Pfam	PF03770. IPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	92097.
SOURCE	Search...

Entry information

Entry name

IPMK_HUMAN

Accession

Primary (citable) accession number: Q8NFU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.