Reviewed, UniProtKB/Swiss-Prot Q8NFT8 (DNER_HUMAN)

Names and origin

Protein names

Recommended name:
    Delta and Notch-like epidermal growth factor-related receptor

Gene names

Name:	DNER
Synonyms:	BET
ORF Names:	UNQ262/PRO299

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis By similarity.
Subunit structure	Interacts with AP1G1. Interacts with NOTCH1 By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Note: Present on the membrane of dendrites and cell bodies but excluded from axonal membrane. Also found in early endosomes in the somatodendritic region By similarity.
Tissue specificity	Expressed in brain, spinal cord and adrenal gland. Ref.7
Sequence similarities	Contains 10 EGF-like domains. Contains 1 follistatin-like domain.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Repeat Signal Transmembrane
Ligand	Calcium
Molecular function	Activator Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	central nervous system development Ref.1 Inferred from expression pattern. Source: UniProtKB endocytosis Ref.1 Non-traceable author statement. Source: UniProtKB neuron migration Ref.1 Non-traceable author statement. Source: UniProtKB synapse assembly Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	dendrite Ref.1 Inferred from direct assay. Source: UniProtKB early endosome Ref.1 Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	calcium ion binding Ref.1 Non-traceable author statement. Source: UniProtKB clathrin binding Ref.1 Traceable author statement. Source: UniProtKB transmembrane receptor activity Ref.1 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 737	703	Delta and Notch-like epidermal growth factor-related receptor		PRO_0000253557
Regions
	Topological domain	35 – 640	606	Extracellular Potential
	Transmembrane	641 – 661	21	Potential
	Topological domain	662 – 737	76	Cytoplasmic Potential
	Domain	44 – 92	49	EGF-like 1
	Domain	94 – 133	40	EGF-like 2
	Domain	309 – 348	40	EGF-like 3
	Domain	349 – 390	42	EGF-like 4
	Domain	392 – 428	37	EGF-like 5
	Domain	430 – 466	37	EGF-like 6
	Domain	468 – 503	36	EGF-like 7
	Domain	505 – 541	37	EGF-like 8; calcium-binding Potential
	Domain	543 – 579	37	EGF-like 9
	Domain	546 – 568	23	Follistatin-like
	Domain	581 – 617	37	EGF-like 10; calcium-binding Potential
	Region	44 – 133	90	Interaction with NOTCH1 By similarity
	Region	677 – 680	4	Interaction with AP1G1 and somatodendritic targeting By similarity
Amino acid modifications
	Modified residue	711	1	Phosphotyrosine By similarity
	Modified residue	718	1	Phosphotyrosine By similarity
	Modified residue	721	1	Phosphotyrosine By similarity
	Glycosylation	223	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	564	1	N-linked (GlcNAc...) Potential
	Disulfide bond	48 ↔ 59		By similarity
	Disulfide bond	53 ↔ 80		By similarity
	Disulfide bond	82 ↔ 91		By similarity
	Disulfide bond	98 ↔ 108		By similarity
	Disulfide bond	103 ↔ 121		By similarity
	Disulfide bond	123 ↔ 132		By similarity
	Disulfide bond	319 ↔ 336		By similarity
	Disulfide bond	338 ↔ 347		By similarity
	Disulfide bond	353 ↔ 364		By similarity
	Disulfide bond	358 ↔ 378		By similarity
	Disulfide bond	380 ↔ 389		By similarity
	Disulfide bond	396 ↔ 407		By similarity
	Disulfide bond	401 ↔ 416		By similarity
	Disulfide bond	418 ↔ 427		By similarity
	Disulfide bond	434 ↔ 445		By similarity
	Disulfide bond	439 ↔ 454		By similarity
	Disulfide bond	456 ↔ 465		By similarity
	Disulfide bond	472 ↔ 482		By similarity
	Disulfide bond	477 ↔ 491		By similarity
	Disulfide bond	493 ↔ 502		By similarity
	Disulfide bond	509 ↔ 520		By similarity
	Disulfide bond	514 ↔ 529		By similarity
	Disulfide bond	531 ↔ 540		By similarity
	Disulfide bond	547 ↔ 558		By similarity
	Disulfide bond	552 ↔ 567		By similarity
	Disulfide bond	569 ↔ 578		By similarity
	Disulfide bond	585 ↔ 596		By similarity
	Disulfide bond	590 ↔ 605		By similarity
	Disulfide bond	607 ↔ 616		By similarity
Natural variations
	Natural variant	433	1	P → L: dbSNP rs17853365. Ref.5		VAR_028380

Sequences

	Sequence		Length	Mass (Da)
	Q8NFT8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 25A8A8A3044308BE	FASTA	737	78,475
	10 20 30 40 50 60 MQPRRAQAPG AQLLPALALL LLLLGAGPRG SSLANPVPAA PLSAPGPCAA QPCRNGGVCT 70 80 90 100 110 120 SRPEPDPQHP APAGEPGYSC TCPAGISGAN CQLVADPCAS NPCHHGNCSS SSSSSSDGYL 130 140 150 160 170 180 CICNEGYEGP NCEQALPSLP ATGWTESMAP RQLQPVPATQ EPDKILPRSQ ATVTLPTWQP 190 200 210 220 230 240 KTGQKVVEMK WDQVEVIPDI ACGNASSNSS AGGRLVSFEV PQNTSVKIRQ DATASLILLW 250 260 270 280 290 300 KVTATGFQQC SLIDGRSVTP LQASGGLVLL EEMLALGNNH FIGFVNDSVT KSIVALRLTL 310 320 330 340 350 360 VVKVSTCVPG ESHANDLECS GKGKCTTKPS EATFSCTCEE QYVGTFCEEY DACQRKPCQN 370 380 390 400 410 420 NASCIDANEK QDGSNFTCVC LPGYTGELCQ SKIDYCILDP CRNGATCISS LSGFTCQCPE 430 440 450 460 470 480 GYFGSACEEK VDPCASSPCQ NNGTCYVDGV HFTCNCSPGF TGPTCAQLID FCALSPCAHG 490 500 510 520 530 540 TCRSVGTSYK CLCDPGYHGL YCEEEYNECL SAPCLNAATC RDLVNGYECV CLAEYKGTHC 550 560 570 580 590 600 ELYKDPCANV SCLNGATCDS DGLNGTCICA PGFTGEECDI DINECDSNPC HHGGSCLDQP 610 620 630 640 650 660 NGYNCHCPHG WVGANCEIHL QWKSGHMAES LTNMPRHSLY IIIGALCVAF ILMLIILIVG 670 680 690 700 710 720 ICRISRIEYQ GSSRPAYEEF YNCRSIDSEF SNAIASIRHA RFGKKSRPAM YDVSPIAYED 730 YSPDDKPLVT LIKTKDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Delta/notch-like epidermal growth factor (EGF)-related receptor, a novel EGF-like repeat-containing protein targeted to dendrites of developing and adult central nervous system neurons." Eiraku M., Hirata Y., Takeshima H., Hirano T., Kengaku M. J. Biol. Chem. 277:25400-25407(2002) [PubMed: 11950833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-433. Tissue: Brain.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 486-737. Tissue: Amygdala.
[7]	"BET, a novel neuronal transmembrane protein with multiple EGF-like motifs." Nishizumi H., Komiyama T., Miyabayashi T., Sakano S., Sakano H. NeuroReport 13:909-915(2002) [PubMed: 11997712] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-223, MASS SPECTROMETRY. Tissue: Saliva.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF442487 mRNA. Translation: AAM21557.1. AY358891 mRNA. Translation: AAQ89250.1. AC007559 Genomic DNA. Translation: AAY14939.1. AC007748 Genomic DNA. Translation: AAY24263.1. AC008273 Genomic DNA. Translation: AAF19247.2. AC093384 Genomic DNA. Translation: AAY14680.1. CH471063 Genomic DNA. Translation: EAW70893.1. BC024766 mRNA. Translation: AAH24766.2. BC035009 mRNA. Translation: AAH35009.1. AL137311 mRNA. Translation: CAB70690.1.
IPI	IPI00333140.
PIR	T46247.
RefSeq	NP_620711.3.
UniGene	Hs.234074
3D structure databases
HSSP	HSSP built from PDB template 1EDM based on UniProtKB P00740.
SMR	Q8NFT8. Positions 380-580, 504-617.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46249N.
STRING	Q8NFT8.
PTM databases
PhosphoSite	Q8NFT8.
Proteomic databases
PRIDE	Q8NFT8.
Genome annotation databases
Ensembl	ENST00000341772; ENSP00000345229; ENSG00000187957; Homo sapiens. [Genome view]
GeneID	92737.
KEGG	hsa:92737.
UCSC	uc002vpv.1. human.
Organism-specific databases
CTD	92737.
GeneCards	GC02M229930.
HGNC	HGNC:24456. DNER.
HPA	HPA017320.
MIM	607299. gene.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15986.
HOVERGEN	Q8NFT8.
InParanoid	Q8NFT8.
OMA	KWDQVEV.
OrthoDB	EOG9BGCFD.
Gene expression databases
ArrayExpress	Q8NFT8.
Bgee	Q8NFT8.
Genevestigator	Q8NFT8.
GermOnline	ENSG00000187957. Homo sapiens.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR001438. EGF_2. IPR000742. EGF_3. IPR001881. EGF_Ca_bd. IPR018097. EGF_Ca_bd_CS. IPR003645. Fol_N. [Graphical view]
PRINTS	PR00010. EGFBLOOD.
SMART	SM00181. EGF. 7 hits. SM00179. EGF_CA. 3 hits. SM00274. FOLN. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 2 hits. PS00022. EGF_1. 10 hits. PS01186. EGF_2. 7 hits. PS50026. EGF_3. 10 hits. PS01187. EGF_CA. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	77861.
SOURCE	Search...

Entry information

Entry name

DNER_HUMAN

Accession

Primary (citable) accession number: Q8NFT8
Secondary accession number(s): A6NP39 , Q53R88, Q53TP7, Q53TQ5, Q8IYT0, Q8TB42, Q9NTF1, Q9UDM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.