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Protein

Protein DBF4 homolog B

Gene

DBF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri294 – 343DBF4-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein kinase activator activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
  • positive regulation of nuclear cell cycle DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog B
Alternative name(s):
Activator of S phase kinase-like protein 1
Short name:
ASK-like protein 1
Chiffon homolog B
Dbf4-related factor 1
Gene namesi
Name:DBF4B
Synonyms:ASKL1, DRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:17883. DBF4B.

Subcellular locationi

GO - Cellular componenti

  • aggresome Source: HPA
  • cytoplasm Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000161692.
PharmGKBiPA143485447.

Polymorphism and mutation databases

BioMutaiDBF4B.
DMDMi74715595.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175531 – 615Protein DBF4 homolog BAdd BLAST615

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8NFT6.
PaxDbiQ8NFT6.
PeptideAtlasiQ8NFT6.
PRIDEiQ8NFT6.

PTM databases

iPTMnetiQ8NFT6.
PhosphoSitePlusiQ8NFT6.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in testis.1 Publication

Developmental stagei

Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000161692.
CleanExiHS_DBF4B.
ExpressionAtlasiQ8NFT6. baseline and differential.
GenevisibleiQ8NFT6. HS.

Organism-specific databases

HPAiHPA048465.

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.

Binary interactionsi

WithEntry#Exp.IntActNotes
LMO2P257913EBI-749662,EBI-739696

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123157. 12 interactors.
IntActiQ8NFT6. 14 interactors.
MINTiMINT-1477188.
STRINGi9606.ENSP00000323663.

Structurei

3D structure databases

ProteinModelPortaliQ8NFT6.
SMRiQ8NFT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 133BRCTAdd BLAST91

Sequence similaritiesi

Contains 1 BRCT domain.Curated
Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri294 – 343DBF4-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOGENOMiHOG000112166.
InParanoidiQ8NFT6.
OMAiPRTSHPC.
OrthoDBiEOG091G06P9.
PhylomeDBiQ8NFT6.
TreeFamiTF332790.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG
60 70 80 90 100
KSFYLDLPAG KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS
110 120 130 140 150
GKSHRGCPSP SPSEVRVETS AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ
160 170 180 190 200
KAIRNQGSIS GGGSGGSSSL LTNARSWGVR ILHVDEMMMH VQQLSLASLC
210 220 230 240 250
VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF RPFHHQFKSF
260 270 280 290 300
PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
310 320 330 340 350
CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ
360 370 380 390 400
AGLPRWSGSP ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG
410 420 430 440 450
RAAGQQRWTE SLDGVMGPPA SHTCVSATTL LPALPKGSRE QGCLCPCPAS
460 470 480 490 500
FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE NSFAPADIPV KGPLLFPEAR
510 520 530 540 550
PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR LGYLYLLLTQ
560 570 580 590 600
SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
610
PQPFLHCGFL AVDSG
Length:615
Mass (Da):67,243
Last modified:October 1, 2002 - v1
Checksum:i02C1F957B60415A4
GO
Isoform 2 (identifier: Q8NFT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     425-431: VSATTLL → HRPCRLP
     432-615: Missing.

Show »
Length:431
Mass (Da):47,177
Checksum:i7E16C13BC9413B46
GO
Isoform 3 (identifier: Q8NFT6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: Missing.
     397-615: VTQGRAAGQQ...HCGFLAVDSG → GIPEQDGTVD...GSSQGQAAPD

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):54,741
Checksum:iAE51A015F4ACD22E
GO
Isoform 4 (identifier: Q8NFT6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-170: GSISGGGSGGSSSL → VSWGKMGQSRWSPA
     171-615: Missing.

Note: No experimental confirmation available.
Show »
Length:170
Mass (Da):18,392
Checksum:iC93EE702A59B282E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207K → R in AAL75985 (Ref. 2) Curated1
Sequence conflicti352G → D in AAL75985 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0310141 – 186Missing in isoform 3. 1 PublicationAdd BLAST186
Alternative sequenceiVSP_031015157 – 170GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_031016171 – 615Missing in isoform 4. 1 PublicationAdd BLAST445
Alternative sequenceiVSP_031017397 – 615VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3. 1 PublicationAdd BLAST219
Alternative sequenceiVSP_031018425 – 431VSATTLL → HRPCRLP in isoform 2. 1 Publication7
Alternative sequenceiVSP_031019432 – 615Missing in isoform 2. 1 PublicationAdd BLAST184

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448801 mRNA. Translation: AAM73808.1.
AF465820 mRNA. Translation: AAL75985.1.
AK023149 mRNA. Translation: BAB14431.1.
CH471178 Genomic DNA. Translation: EAW51581.1.
CH471178 Genomic DNA. Translation: EAW51584.1.
CH471178 Genomic DNA. Translation: EAW51587.1.
CH471178 Genomic DNA. Translation: EAW51582.1.
CH471178 Genomic DNA. Translation: EAW51586.1.
BC016158 mRNA. Translation: AAH16158.1.
CCDSiCCDS11485.1. [Q8NFT6-1]
CCDS45706.2. [Q8NFT6-2]
RefSeqiNP_079380.2. NM_025104.3. [Q8NFT6-2]
NP_663696.1. NM_145663.2. [Q8NFT6-1]
XP_006722165.1. XM_006722102.3. [Q8NFT6-3]
XP_011523583.1. XM_011525281.2. [Q8NFT6-3]
UniGeneiHs.369998.

Genome annotation databases

EnsembliENST00000315005; ENSP00000323663; ENSG00000161692. [Q8NFT6-1]
ENST00000393547; ENSP00000377178; ENSG00000161692. [Q8NFT6-2]
GeneIDi80174.
KEGGihsa:80174.
UCSCiuc002ihf.4. human. [Q8NFT6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448801 mRNA. Translation: AAM73808.1.
AF465820 mRNA. Translation: AAL75985.1.
AK023149 mRNA. Translation: BAB14431.1.
CH471178 Genomic DNA. Translation: EAW51581.1.
CH471178 Genomic DNA. Translation: EAW51584.1.
CH471178 Genomic DNA. Translation: EAW51587.1.
CH471178 Genomic DNA. Translation: EAW51582.1.
CH471178 Genomic DNA. Translation: EAW51586.1.
BC016158 mRNA. Translation: AAH16158.1.
CCDSiCCDS11485.1. [Q8NFT6-1]
CCDS45706.2. [Q8NFT6-2]
RefSeqiNP_079380.2. NM_025104.3. [Q8NFT6-2]
NP_663696.1. NM_145663.2. [Q8NFT6-1]
XP_006722165.1. XM_006722102.3. [Q8NFT6-3]
XP_011523583.1. XM_011525281.2. [Q8NFT6-3]
UniGeneiHs.369998.

3D structure databases

ProteinModelPortaliQ8NFT6.
SMRiQ8NFT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123157. 12 interactors.
IntActiQ8NFT6. 14 interactors.
MINTiMINT-1477188.
STRINGi9606.ENSP00000323663.

PTM databases

iPTMnetiQ8NFT6.
PhosphoSitePlusiQ8NFT6.

Polymorphism and mutation databases

BioMutaiDBF4B.
DMDMi74715595.

Proteomic databases

MaxQBiQ8NFT6.
PaxDbiQ8NFT6.
PeptideAtlasiQ8NFT6.
PRIDEiQ8NFT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315005; ENSP00000323663; ENSG00000161692. [Q8NFT6-1]
ENST00000393547; ENSP00000377178; ENSG00000161692. [Q8NFT6-2]
GeneIDi80174.
KEGGihsa:80174.
UCSCiuc002ihf.4. human. [Q8NFT6-1]

Organism-specific databases

CTDi80174.
GeneCardsiDBF4B.
HGNCiHGNC:17883. DBF4B.
HPAiHPA048465.
MIMi611661. gene.
neXtProtiNX_Q8NFT6.
OpenTargetsiENSG00000161692.
PharmGKBiPA143485447.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOGENOMiHOG000112166.
InParanoidiQ8NFT6.
OMAiPRTSHPC.
OrthoDBiEOG091G06P9.
PhylomeDBiQ8NFT6.
TreeFamiTF332790.

Miscellaneous databases

GenomeRNAii80174.
PROiQ8NFT6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161692.
CleanExiHS_DBF4B.
ExpressionAtlasiQ8NFT6. baseline and differential.
GenevisibleiQ8NFT6. HS.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBF4B_HUMAN
AccessioniPrimary (citable) accession number: Q8NFT6
Secondary accession number(s): D3DX56
, Q8TEX0, Q96B19, Q9H912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.