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Q8NFT6 (DBF4B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein DBF4 homolog B
Alternative name(s):
Activator of S phase kinase-like protein 1
Short name=ASK-like protein 1
Chiffon homolog B
Dbf4-related factor 1
Gene names
Name:DBF4B
Synonyms:ASKL1, DRF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. Ref.1 Ref.6 Ref.7

Subunit structure

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.

Subcellular location

Nucleus. Note: Predominantly found in soluble fraction but not in the chromatin-bound fraction. Ref.1 Ref.6

Tissue specificity

Widely expressed. Highly expressed in testis. Ref.1

Developmental stage

Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level). Ref.1 Ref.6

Post-translational modification

Phosphorylated. Ref.1

Sequence similarities

Contains 1 BRCT domain.

Contains 1 DBF4-type zinc finger.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentaggresome

Inferred from direct assay. Source: HPA

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NFT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NFT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     425-431: VSATTLL → HRPCRLP
     432-615: Missing.
Isoform 3 (identifier: Q8NFT6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: Missing.
     397-615: VTQGRAAGQQ...HCGFLAVDSG → GIPEQDGTVD...GSSQGQAAPD
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8NFT6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     157-170: GSISGGGSGGSSSL → VSWGKMGQSRWSPA
     171-615: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615Protein DBF4 homolog B
PRO_0000317553

Regions

Domain43 – 13391BRCT
Zinc finger294 – 34350DBF4-type

Natural variations

Alternative sequence1 – 186186Missing in isoform 3.
VSP_031014
Alternative sequence157 – 17014GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4.
VSP_031015
Alternative sequence171 – 615445Missing in isoform 4.
VSP_031016
Alternative sequence397 – 615219VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3.
VSP_031017
Alternative sequence425 – 4317VSATTLL → HRPCRLP in isoform 2.
VSP_031018
Alternative sequence432 – 615184Missing in isoform 2.
VSP_031019

Experimental info

Sequence conflict2071K → R in AAL75985. Ref.2
Sequence conflict3521G → D in AAL75985. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 02C1F957B60415A4

FASTA61567,243
        10         20         30         40         50         60 
MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG KSFYLDLPAG 

        70         80         90        100        110        120 
KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS GKSHRGCPSP SPSEVRVETS 

       130        140        150        160        170        180 
AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ KAIRNQGSIS GGGSGGSSSL LTNARSWGVR 

       190        200        210        220        230        240 
ILHVDEMMMH VQQLSLASLC VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF 

       250        260        270        280        290        300 
RPFHHQFKSF PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY 

       310        320        330        340        350        360 
CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ AGLPRWSGSP 

       370        380        390        400        410        420 
ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG RAAGQQRWTE SLDGVMGPPA 

       430        440        450        460        470        480 
SHTCVSATTL LPALPKGSRE QGCLCPCPAS FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE 

       490        500        510        520        530        540 
NSFAPADIPV KGPLLFPEAR PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR 

       550        560        570        580        590        600 
LGYLYLLLTQ SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT 

       610 
PQPFLHCGFL AVDSG 

« Hide

Isoform 2 [UniParc].

Checksum: 7E16C13BC9413B46
Show »

FASTA43147,177
Isoform 3 [UniParc].

Checksum: AE51A015F4ACD22E
Show »

FASTA50854,741
Isoform 4 [UniParc].

Checksum: C93EE702A59B282E
Show »

FASTA17018,392

References

« Hide 'large scale' references
[1]"Drf1, a novel regulatory subunit for human Cdc7 kinase."
Montagnoli A., Bosotti R., Villa F., Rialland M., Brotherton D., Mercurio C., Berthelsen J., Santocanale C.
EMBO J. 21:3171-3181(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
[2]Shannon M.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[6]"A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases."
Yoshizawa-Sugata N., Ishii A., Taniyama C., Matsui E., Arai K., Masai H.
J. Biol. Chem. 280:13062-13070(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
[7]"Cdc7 is an active kinase in human cancer cells undergoing replication stress."
Tenca P., Brotherton D., Montagnoli A., Rainoldi S., Albanese C., Santocanale C.
J. Biol. Chem. 282:208-215(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF448801 mRNA. Translation: AAM73808.1.
AF465820 mRNA. Translation: AAL75985.1.
AK023149 mRNA. Translation: BAB14431.1.
CH471178 Genomic DNA. Translation: EAW51581.1.
CH471178 Genomic DNA. Translation: EAW51584.1.
CH471178 Genomic DNA. Translation: EAW51587.1.
CH471178 Genomic DNA. Translation: EAW51582.1.
CH471178 Genomic DNA. Translation: EAW51586.1.
BC016158 mRNA. Translation: AAH16158.1.
RefSeqNP_079380.2. NM_025104.3.
NP_663696.1. NM_145663.2.
UniGeneHs.369998.

3D structure databases

ProteinModelPortalQ8NFT6.
SMRQ8NFT6. Positions 225-346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123157. 8 interactions.
IntActQ8NFT6. 5 interactions.
MINTMINT-1477188.
STRING9606.ENSP00000323663.

PTM databases

PhosphoSiteQ8NFT6.

Polymorphism databases

DMDM74715595.

Proteomic databases

PaxDbQ8NFT6.
PRIDEQ8NFT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000315005; ENSP00000323663; ENSG00000161692. [Q8NFT6-1]
ENST00000393547; ENSP00000377178; ENSG00000161692. [Q8NFT6-2]
GeneID80174.
KEGGhsa:80174.
UCSCuc002ihe.3. human. [Q8NFT6-3]
uc002ihf.3. human. [Q8NFT6-1]
uc010wjc.2. human. [Q8NFT6-2]

Organism-specific databases

CTD80174.
GeneCardsGC17P042785.
HGNCHGNC:17883. DBF4B.
HPAHPA048465.
MIM611661. gene.
neXtProtNX_Q8NFT6.
PharmGKBPA143485447.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG314450.
HOGENOMHOG000112166.
InParanoidQ8NFT6.
OMAPRTSHPC.
OrthoDBEOG7BGHK5.
PhylomeDBQ8NFT6.
TreeFamTF332790.

Gene expression databases

ArrayExpressQ8NFT6.
BgeeQ8NFT6.
CleanExHS_DBF4B.
GenevestigatorQ8NFT6.

Family and domain databases

Gene3D3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEPS51265. ZF_DBF4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi80174.
NextBio70499.
PROQ8NFT6.
SOURCESearch...

Entry information

Entry nameDBF4B_HUMAN
AccessionPrimary (citable) accession number: Q8NFT6
Secondary accession number(s): D3DX56 expand/collapse secondary AC list , Q8TEX0, Q96B19, Q9H912
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM