Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8NFT6

- DBF4B_HUMAN

UniProt

Q8NFT6 - DBF4B_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein DBF4 homolog B

Gene

DBF4B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri294 – 34350DBF4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein kinase activator activity Source: UniProt
  3. protein kinase binding Source: UniProt
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. positive regulation of cell proliferation Source: UniProt
  3. positive regulation of G2/M transition of mitotic cell cycle Source: UniProt
  4. positive regulation of nuclear cell cycle DNA replication Source: UniProt
  5. positive regulation of protein kinase activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog B
Alternative name(s):
Activator of S phase kinase-like protein 1
Short name:
ASK-like protein 1
Chiffon homolog B
Dbf4-related factor 1
Gene namesi
Name:DBF4B
Synonyms:ASKL1, DRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:17883. DBF4B.

Subcellular locationi

Nucleus 2 Publications
Note: Predominantly found in soluble fraction but not in the chromatin-bound fraction.

GO - Cellular componenti

  1. aggresome Source: HPA
  2. cytoplasm Source: UniProt
  3. intracellular membrane-bounded organelle Source: HPA
  4. nucleoplasm Source: UniProt
  5. nucleus Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485447.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Protein DBF4 homolog BPRO_0000317553Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8NFT6.
PaxDbiQ8NFT6.
PRIDEiQ8NFT6.

PTM databases

PhosphoSiteiQ8NFT6.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in testis.1 Publication

Developmental stagei

Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).2 Publications

Gene expression databases

BgeeiQ8NFT6.
CleanExiHS_DBF4B.
ExpressionAtlasiQ8NFT6. baseline and differential.
GenevestigatoriQ8NFT6.

Organism-specific databases

HPAiHPA048465.

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.

Protein-protein interaction databases

BioGridi123157. 11 interactions.
IntActiQ8NFT6. 7 interactions.
MINTiMINT-1477188.
STRINGi9606.ENSP00000323663.

Structurei

3D structure databases

ProteinModelPortaliQ8NFT6.
SMRiQ8NFT6. Positions 225-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 13391BRCTAdd
BLAST

Sequence similaritiesi

Contains 1 BRCT domain.Curated
Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri294 – 34350DBF4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG314450.
GeneTreeiENSGT00530000063909.
HOGENOMiHOG000112166.
InParanoidiQ8NFT6.
OMAiKCQKNSP.
OrthoDBiEOG7BGHK5.
PhylomeDBiQ8NFT6.
TreeFamiTF332790.

Family and domain databases

Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8NFT6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG
60 70 80 90 100
KSFYLDLPAG KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS
110 120 130 140 150
GKSHRGCPSP SPSEVRVETS AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ
160 170 180 190 200
KAIRNQGSIS GGGSGGSSSL LTNARSWGVR ILHVDEMMMH VQQLSLASLC
210 220 230 240 250
VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF RPFHHQFKSF
260 270 280 290 300
PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
310 320 330 340 350
CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ
360 370 380 390 400
AGLPRWSGSP ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG
410 420 430 440 450
RAAGQQRWTE SLDGVMGPPA SHTCVSATTL LPALPKGSRE QGCLCPCPAS
460 470 480 490 500
FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE NSFAPADIPV KGPLLFPEAR
510 520 530 540 550
PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR LGYLYLLLTQ
560 570 580 590 600
SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
610
PQPFLHCGFL AVDSG
Length:615
Mass (Da):67,243
Last modified:October 1, 2002 - v1
Checksum:i02C1F957B60415A4
GO
Isoform 2 (identifier: Q8NFT6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     425-431: VSATTLL → HRPCRLP
     432-615: Missing.

Show »
Length:431
Mass (Da):47,177
Checksum:i7E16C13BC9413B46
GO
Isoform 3 (identifier: Q8NFT6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: Missing.
     397-615: VTQGRAAGQQ...HCGFLAVDSG → GIPEQDGTVD...GSSQGQAAPD

Note: No experimental confirmation available.

Show »
Length:508
Mass (Da):54,741
Checksum:iAE51A015F4ACD22E
GO
Isoform 4 (identifier: Q8NFT6-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-170: GSISGGGSGGSSSL → VSWGKMGQSRWSPA
     171-615: Missing.

Note: No experimental confirmation available.

Show »
Length:170
Mass (Da):18,392
Checksum:iC93EE702A59B282E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071K → R in AAL75985. 1 PublicationCurated
Sequence conflicti352 – 3521G → D in AAL75985. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 186186Missing in isoform 3. 1 PublicationVSP_031014Add
BLAST
Alternative sequencei157 – 17014GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4. 1 PublicationVSP_031015Add
BLAST
Alternative sequencei171 – 615445Missing in isoform 4. 1 PublicationVSP_031016Add
BLAST
Alternative sequencei397 – 615219VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3. 1 PublicationVSP_031017Add
BLAST
Alternative sequencei425 – 4317VSATTLL → HRPCRLP in isoform 2. 1 PublicationVSP_031018
Alternative sequencei432 – 615184Missing in isoform 2. 1 PublicationVSP_031019Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448801 mRNA. Translation: AAM73808.1.
AF465820 mRNA. Translation: AAL75985.1.
AK023149 mRNA. Translation: BAB14431.1.
CH471178 Genomic DNA. Translation: EAW51581.1.
CH471178 Genomic DNA. Translation: EAW51584.1.
CH471178 Genomic DNA. Translation: EAW51587.1.
CH471178 Genomic DNA. Translation: EAW51582.1.
CH471178 Genomic DNA. Translation: EAW51586.1.
BC016158 mRNA. Translation: AAH16158.1.
CCDSiCCDS11485.1. [Q8NFT6-1]
CCDS45706.2. [Q8NFT6-2]
RefSeqiNP_079380.2. NM_025104.3. [Q8NFT6-2]
NP_663696.1. NM_145663.2. [Q8NFT6-1]
XP_006722165.1. XM_006722102.1. [Q8NFT6-3]
UniGeneiHs.369998.

Genome annotation databases

EnsembliENST00000315005; ENSP00000323663; ENSG00000161692. [Q8NFT6-1]
ENST00000393547; ENSP00000377178; ENSG00000161692. [Q8NFT6-2]
GeneIDi80174.
KEGGihsa:80174.
UCSCiuc002ihe.3. human. [Q8NFT6-3]
uc002ihf.3. human. [Q8NFT6-1]
uc010wjc.2. human. [Q8NFT6-2]

Polymorphism databases

DMDMi74715595.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448801 mRNA. Translation: AAM73808.1 .
AF465820 mRNA. Translation: AAL75985.1 .
AK023149 mRNA. Translation: BAB14431.1 .
CH471178 Genomic DNA. Translation: EAW51581.1 .
CH471178 Genomic DNA. Translation: EAW51584.1 .
CH471178 Genomic DNA. Translation: EAW51587.1 .
CH471178 Genomic DNA. Translation: EAW51582.1 .
CH471178 Genomic DNA. Translation: EAW51586.1 .
BC016158 mRNA. Translation: AAH16158.1 .
CCDSi CCDS11485.1. [Q8NFT6-1 ]
CCDS45706.2. [Q8NFT6-2 ]
RefSeqi NP_079380.2. NM_025104.3. [Q8NFT6-2 ]
NP_663696.1. NM_145663.2. [Q8NFT6-1 ]
XP_006722165.1. XM_006722102.1. [Q8NFT6-3 ]
UniGenei Hs.369998.

3D structure databases

ProteinModelPortali Q8NFT6.
SMRi Q8NFT6. Positions 225-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123157. 11 interactions.
IntActi Q8NFT6. 7 interactions.
MINTi MINT-1477188.
STRINGi 9606.ENSP00000323663.

PTM databases

PhosphoSitei Q8NFT6.

Polymorphism databases

DMDMi 74715595.

Proteomic databases

MaxQBi Q8NFT6.
PaxDbi Q8NFT6.
PRIDEi Q8NFT6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000315005 ; ENSP00000323663 ; ENSG00000161692 . [Q8NFT6-1 ]
ENST00000393547 ; ENSP00000377178 ; ENSG00000161692 . [Q8NFT6-2 ]
GeneIDi 80174.
KEGGi hsa:80174.
UCSCi uc002ihe.3. human. [Q8NFT6-3 ]
uc002ihf.3. human. [Q8NFT6-1 ]
uc010wjc.2. human. [Q8NFT6-2 ]

Organism-specific databases

CTDi 80174.
GeneCardsi GC17P042785.
HGNCi HGNC:17883. DBF4B.
HPAi HPA048465.
MIMi 611661. gene.
neXtProti NX_Q8NFT6.
PharmGKBi PA143485447.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG314450.
GeneTreei ENSGT00530000063909.
HOGENOMi HOG000112166.
InParanoidi Q8NFT6.
OMAi KCQKNSP.
OrthoDBi EOG7BGHK5.
PhylomeDBi Q8NFT6.
TreeFami TF332790.

Miscellaneous databases

GenomeRNAii 80174.
NextBioi 70499.
PROi Q8NFT6.
SOURCEi Search...

Gene expression databases

Bgeei Q8NFT6.
CleanExi HS_DBF4B.
ExpressionAtlasi Q8NFT6. baseline and differential.
Genevestigatori Q8NFT6.

Family and domain databases

Gene3Di 3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view ]
Pfami PF07535. zf-DBF. 1 hit.
[Graphical view ]
SMARTi SM00586. ZnF_DBF. 1 hit.
[Graphical view ]
PROSITEi PS51265. ZF_DBF4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
  2. Shannon M.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  6. "A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases."
    Yoshizawa-Sugata N., Ishii A., Taniyama C., Matsui E., Arai K., Masai H.
    J. Biol. Chem. 280:13062-13070(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
  7. "Cdc7 is an active kinase in human cancer cells undergoing replication stress."
    Tenca P., Brotherton D., Montagnoli A., Rainoldi S., Albanese C., Santocanale C.
    J. Biol. Chem. 282:208-215(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC7.

Entry informationi

Entry nameiDBF4B_HUMAN
AccessioniPrimary (citable) accession number: Q8NFT6
Secondary accession number(s): D3DX56
, Q8TEX0, Q96B19, Q9H912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3