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Q8NFT6

- DBF4B_HUMAN

UniProt

Q8NFT6 - DBF4B_HUMAN

Protein

Protein DBF4 homolog B

Gene

DBF4B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri294 – 34350DBF4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. protein kinase activator activity Source: UniProt
    3. protein kinase binding Source: UniProt
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. positive regulation of cell proliferation Source: UniProt
    3. positive regulation of G2/M transition of mitotic cell cycle Source: UniProt
    4. positive regulation of nuclear cell cycle DNA replication Source: UniProt
    5. positive regulation of protein kinase activity Source: GOC

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein DBF4 homolog B
    Alternative name(s):
    Activator of S phase kinase-like protein 1
    Short name:
    ASK-like protein 1
    Chiffon homolog B
    Dbf4-related factor 1
    Gene namesi
    Name:DBF4B
    Synonyms:ASKL1, DRF1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:17883. DBF4B.

    Subcellular locationi

    Nucleus 2 Publications
    Note: Predominantly found in soluble fraction but not in the chromatin-bound fraction.

    GO - Cellular componenti

    1. aggresome Source: HPA
    2. cytoplasm Source: UniProt
    3. intracellular membrane-bounded organelle Source: HPA
    4. nucleoplasm Source: UniProt
    5. nucleus Source: UniProt

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA143485447.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 615615Protein DBF4 homolog BPRO_0000317553Add
    BLAST

    Post-translational modificationi

    Phosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8NFT6.
    PaxDbiQ8NFT6.
    PRIDEiQ8NFT6.

    PTM databases

    PhosphoSiteiQ8NFT6.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in testis.1 Publication

    Developmental stagei

    Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).2 Publications

    Gene expression databases

    ArrayExpressiQ8NFT6.
    BgeeiQ8NFT6.
    CleanExiHS_DBF4B.
    GenevestigatoriQ8NFT6.

    Organism-specific databases

    HPAiHPA048465.

    Interactioni

    Subunit structurei

    Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.

    Protein-protein interaction databases

    BioGridi123157. 8 interactions.
    IntActiQ8NFT6. 6 interactions.
    MINTiMINT-1477188.
    STRINGi9606.ENSP00000323663.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NFT6.
    SMRiQ8NFT6. Positions 225-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 13391BRCTAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BRCT domain.Curated
    Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri294 – 34350DBF4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG314450.
    HOGENOMiHOG000112166.
    InParanoidiQ8NFT6.
    OMAiKCQKNSP.
    OrthoDBiEOG7BGHK5.
    PhylomeDBiQ8NFT6.
    TreeFamiTF332790.

    Family and domain databases

    Gene3Di3.40.50.10190. 2 hits.
    InterProiIPR001357. BRCT_dom.
    IPR006572. Znf_DBF.
    [Graphical view]
    PfamiPF07535. zf-DBF. 1 hit.
    [Graphical view]
    SMARTiSM00586. ZnF_DBF. 1 hit.
    [Graphical view]
    PROSITEiPS51265. ZF_DBF4. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NFT6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG    50
    KSFYLDLPAG KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS 100
    GKSHRGCPSP SPSEVRVETS AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ 150
    KAIRNQGSIS GGGSGGSSSL LTNARSWGVR ILHVDEMMMH VQQLSLASLC 200
    VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF RPFHHQFKSF 250
    PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY 300
    CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ 350
    AGLPRWSGSP ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG 400
    RAAGQQRWTE SLDGVMGPPA SHTCVSATTL LPALPKGSRE QGCLCPCPAS 450
    FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE NSFAPADIPV KGPLLFPEAR 500
    PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR LGYLYLLLTQ 550
    SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT 600
    PQPFLHCGFL AVDSG 615
    Length:615
    Mass (Da):67,243
    Last modified:October 1, 2002 - v1
    Checksum:i02C1F957B60415A4
    GO
    Isoform 2 (identifier: Q8NFT6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         425-431: VSATTLL → HRPCRLP
         432-615: Missing.

    Show »
    Length:431
    Mass (Da):47,177
    Checksum:i7E16C13BC9413B46
    GO
    Isoform 3 (identifier: Q8NFT6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-186: Missing.
         397-615: VTQGRAAGQQ...HCGFLAVDSG → GIPEQDGTVD...GSSQGQAAPD

    Note: No experimental confirmation available.

    Show »
    Length:508
    Mass (Da):54,741
    Checksum:iAE51A015F4ACD22E
    GO
    Isoform 4 (identifier: Q8NFT6-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         157-170: GSISGGGSGGSSSL → VSWGKMGQSRWSPA
         171-615: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:170
    Mass (Da):18,392
    Checksum:iC93EE702A59B282E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti207 – 2071K → R in AAL75985. 1 PublicationCurated
    Sequence conflicti352 – 3521G → D in AAL75985. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 186186Missing in isoform 3. 1 PublicationVSP_031014Add
    BLAST
    Alternative sequencei157 – 17014GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4. 1 PublicationVSP_031015Add
    BLAST
    Alternative sequencei171 – 615445Missing in isoform 4. 1 PublicationVSP_031016Add
    BLAST
    Alternative sequencei397 – 615219VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3. 1 PublicationVSP_031017Add
    BLAST
    Alternative sequencei425 – 4317VSATTLL → HRPCRLP in isoform 2. 1 PublicationVSP_031018
    Alternative sequencei432 – 615184Missing in isoform 2. 1 PublicationVSP_031019Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448801 mRNA. Translation: AAM73808.1.
    AF465820 mRNA. Translation: AAL75985.1.
    AK023149 mRNA. Translation: BAB14431.1.
    CH471178 Genomic DNA. Translation: EAW51581.1.
    CH471178 Genomic DNA. Translation: EAW51584.1.
    CH471178 Genomic DNA. Translation: EAW51587.1.
    CH471178 Genomic DNA. Translation: EAW51582.1.
    CH471178 Genomic DNA. Translation: EAW51586.1.
    BC016158 mRNA. Translation: AAH16158.1.
    CCDSiCCDS11485.1. [Q8NFT6-1]
    CCDS45706.2. [Q8NFT6-2]
    RefSeqiNP_079380.2. NM_025104.3. [Q8NFT6-2]
    NP_663696.1. NM_145663.2. [Q8NFT6-1]
    XP_006722165.1. XM_006722102.1. [Q8NFT6-3]
    UniGeneiHs.369998.

    Genome annotation databases

    EnsembliENST00000315005; ENSP00000323663; ENSG00000161692. [Q8NFT6-1]
    ENST00000393547; ENSP00000377178; ENSG00000161692. [Q8NFT6-2]
    GeneIDi80174.
    KEGGihsa:80174.
    UCSCiuc002ihe.3. human. [Q8NFT6-3]
    uc002ihf.3. human. [Q8NFT6-1]
    uc010wjc.2. human. [Q8NFT6-2]

    Polymorphism databases

    DMDMi74715595.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448801 mRNA. Translation: AAM73808.1 .
    AF465820 mRNA. Translation: AAL75985.1 .
    AK023149 mRNA. Translation: BAB14431.1 .
    CH471178 Genomic DNA. Translation: EAW51581.1 .
    CH471178 Genomic DNA. Translation: EAW51584.1 .
    CH471178 Genomic DNA. Translation: EAW51587.1 .
    CH471178 Genomic DNA. Translation: EAW51582.1 .
    CH471178 Genomic DNA. Translation: EAW51586.1 .
    BC016158 mRNA. Translation: AAH16158.1 .
    CCDSi CCDS11485.1. [Q8NFT6-1 ]
    CCDS45706.2. [Q8NFT6-2 ]
    RefSeqi NP_079380.2. NM_025104.3. [Q8NFT6-2 ]
    NP_663696.1. NM_145663.2. [Q8NFT6-1 ]
    XP_006722165.1. XM_006722102.1. [Q8NFT6-3 ]
    UniGenei Hs.369998.

    3D structure databases

    ProteinModelPortali Q8NFT6.
    SMRi Q8NFT6. Positions 225-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123157. 8 interactions.
    IntActi Q8NFT6. 6 interactions.
    MINTi MINT-1477188.
    STRINGi 9606.ENSP00000323663.

    PTM databases

    PhosphoSitei Q8NFT6.

    Polymorphism databases

    DMDMi 74715595.

    Proteomic databases

    MaxQBi Q8NFT6.
    PaxDbi Q8NFT6.
    PRIDEi Q8NFT6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000315005 ; ENSP00000323663 ; ENSG00000161692 . [Q8NFT6-1 ]
    ENST00000393547 ; ENSP00000377178 ; ENSG00000161692 . [Q8NFT6-2 ]
    GeneIDi 80174.
    KEGGi hsa:80174.
    UCSCi uc002ihe.3. human. [Q8NFT6-3 ]
    uc002ihf.3. human. [Q8NFT6-1 ]
    uc010wjc.2. human. [Q8NFT6-2 ]

    Organism-specific databases

    CTDi 80174.
    GeneCardsi GC17P042785.
    HGNCi HGNC:17883. DBF4B.
    HPAi HPA048465.
    MIMi 611661. gene.
    neXtProti NX_Q8NFT6.
    PharmGKBi PA143485447.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG314450.
    HOGENOMi HOG000112166.
    InParanoidi Q8NFT6.
    OMAi KCQKNSP.
    OrthoDBi EOG7BGHK5.
    PhylomeDBi Q8NFT6.
    TreeFami TF332790.

    Miscellaneous databases

    GenomeRNAii 80174.
    NextBioi 70499.
    PROi Q8NFT6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8NFT6.
    Bgeei Q8NFT6.
    CleanExi HS_DBF4B.
    Genevestigatori Q8NFT6.

    Family and domain databases

    Gene3Di 3.40.50.10190. 2 hits.
    InterProi IPR001357. BRCT_dom.
    IPR006572. Znf_DBF.
    [Graphical view ]
    Pfami PF07535. zf-DBF. 1 hit.
    [Graphical view ]
    SMARTi SM00586. ZnF_DBF. 1 hit.
    [Graphical view ]
    PROSITEi PS51265. ZF_DBF4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
    2. Shannon M.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lung.
    6. "A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases."
      Yoshizawa-Sugata N., Ishii A., Taniyama C., Matsui E., Arai K., Masai H.
      J. Biol. Chem. 280:13062-13070(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CDC7.
    7. "Cdc7 is an active kinase in human cancer cells undergoing replication stress."
      Tenca P., Brotherton D., Montagnoli A., Rainoldi S., Albanese C., Santocanale C.
      J. Biol. Chem. 282:208-215(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC7.

    Entry informationi

    Entry nameiDBF4B_HUMAN
    AccessioniPrimary (citable) accession number: Q8NFT6
    Secondary accession number(s): D3DX56
    , Q8TEX0, Q96B19, Q9H912
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3