Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein DBF4 homolog B

Gene

DBF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri294 – 343DBF4-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein kinase activator activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
  • positive regulation of nuclear cell cycle DNA replication Source: UniProtKB

Keywordsi

Biological processCell cycle
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog B
Alternative name(s):
Activator of S phase kinase-like protein 1
Short name:
ASK-like protein 1
Chiffon homolog B
Dbf4-related factor 1
Gene namesi
Name:DBF4B
Synonyms:ASKL1, DRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000161692.17
HGNCiHGNC:17883 DBF4B
MIMi611661 gene
neXtProtiNX_Q8NFT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000161692
PharmGKBiPA143485447

Polymorphism and mutation databases

BioMutaiDBF4B
DMDMi74715595

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175531 – 615Protein DBF4 homolog BAdd BLAST615

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8NFT6
PaxDbiQ8NFT6
PeptideAtlasiQ8NFT6
PRIDEiQ8NFT6

PTM databases

iPTMnetiQ8NFT6
PhosphoSitePlusiQ8NFT6

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in testis.1 Publication

Developmental stagei

Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000161692
CleanExiHS_DBF4B
ExpressionAtlasiQ8NFT6 baseline and differential
GenevisibleiQ8NFT6 HS

Organism-specific databases

HPAiHPA048465

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.

Binary interactionsi

WithEntry#Exp.IntActNotes
LMO2P257913EBI-749662,EBI-739696

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi12315712 interactors.
IntActiQ8NFT6 15 interactors.
MINTiQ8NFT6
STRINGi9606.ENSP00000323663

Structurei

3D structure databases

ProteinModelPortaliQ8NFT6
SMRiQ8NFT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 133BRCTAdd BLAST91

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri294 – 343DBF4-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4139 Eukaryota
COG5067 LUCA
GeneTreeiENSGT00530000063909
HOGENOMiHOG000112166
InParanoidiQ8NFT6
OMAiHVDEMMM
OrthoDBiEOG091G06P9
PhylomeDBiQ8NFT6
TreeFamiTF332790

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.30.160.6801 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR006572 Znf_DBF
IPR038545 Znf_DBF_sf
PfamiView protein in Pfam
PF07535 zf-DBF, 1 hit
SMARTiView protein in SMART
SM00586 ZnF_DBF, 1 hit
PROSITEiView protein in PROSITE
PS51265 ZF_DBF4, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG
60 70 80 90 100
KSFYLDLPAG KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS
110 120 130 140 150
GKSHRGCPSP SPSEVRVETS AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ
160 170 180 190 200
KAIRNQGSIS GGGSGGSSSL LTNARSWGVR ILHVDEMMMH VQQLSLASLC
210 220 230 240 250
VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF RPFHHQFKSF
260 270 280 290 300
PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
310 320 330 340 350
CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ
360 370 380 390 400
AGLPRWSGSP ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG
410 420 430 440 450
RAAGQQRWTE SLDGVMGPPA SHTCVSATTL LPALPKGSRE QGCLCPCPAS
460 470 480 490 500
FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE NSFAPADIPV KGPLLFPEAR
510 520 530 540 550
PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR LGYLYLLLTQ
560 570 580 590 600
SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
610
PQPFLHCGFL AVDSG
Length:615
Mass (Da):67,243
Last modified:October 1, 2002 - v1
Checksum:i02C1F957B60415A4
GO
Isoform 2 (identifier: Q8NFT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     425-431: VSATTLL → HRPCRLP
     432-615: Missing.

Show »
Length:431
Mass (Da):47,177
Checksum:i7E16C13BC9413B46
GO
Isoform 3 (identifier: Q8NFT6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: Missing.
     397-615: VTQGRAAGQQ...HCGFLAVDSG → GIPEQDGTVD...GSSQGQAAPD

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):54,741
Checksum:iAE51A015F4ACD22E
GO
Isoform 4 (identifier: Q8NFT6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-170: GSISGGGSGGSSSL → VSWGKMGQSRWSPA
     171-615: Missing.

Note: No experimental confirmation available.
Show »
Length:170
Mass (Da):18,392
Checksum:iC93EE702A59B282E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207K → R in AAL75985 (Ref. 2) Curated1
Sequence conflicti352G → D in AAL75985 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0310141 – 186Missing in isoform 3. 1 PublicationAdd BLAST186
Alternative sequenceiVSP_031015157 – 170GSISG…GSSSL → VSWGKMGQSRWSPA in isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_031016171 – 615Missing in isoform 4. 1 PublicationAdd BLAST445
Alternative sequenceiVSP_031017397 – 615VTQGR…AVDSG → GIPEQDGTVDSTQAPAERAG TGEVPGPIASCQDLGVSVDV FVDPPGIPVSRSPACQCLLP SSGFMELSSGPDLALFGHKR KVQFPSGSAKKRVGASWPQA SFFVPIAPNPCGTRTTSGKR LPSLPLTGHESRLLASLQPL CHSQTCLSLPDPFPWQPTDR PAEFWATQPSWLGKGWPPGP EDSECTATGPVSQEAGQLLS CPTAPGWPSAPLYSATSVQP SGAPVESRSTSLLQPLPASA GASCSRCLWAPQPLQVPCLP VSQPWSQPQPQPQPHAGREL LLRVPKVLGSSQGQAAPD in isoform 3. 1 PublicationAdd BLAST219
Alternative sequenceiVSP_031018425 – 431VSATTLL → HRPCRLP in isoform 2. 1 Publication7
Alternative sequenceiVSP_031019432 – 615Missing in isoform 2. 1 PublicationAdd BLAST184

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448801 mRNA Translation: AAM73808.1
AF465820 mRNA Translation: AAL75985.1
AK023149 mRNA Translation: BAB14431.1
CH471178 Genomic DNA Translation: EAW51581.1
CH471178 Genomic DNA Translation: EAW51584.1
CH471178 Genomic DNA Translation: EAW51587.1
CH471178 Genomic DNA Translation: EAW51582.1
CH471178 Genomic DNA Translation: EAW51586.1
BC016158 mRNA Translation: AAH16158.1
CCDSiCCDS11485.1 [Q8NFT6-1]
CCDS45706.2 [Q8NFT6-2]
RefSeqiNP_079380.2, NM_025104.3 [Q8NFT6-2]
NP_663696.1, NM_145663.2 [Q8NFT6-1]
XP_006722165.1, XM_006722102.3 [Q8NFT6-3]
XP_011523583.1, XM_011525281.2 [Q8NFT6-3]
UniGeneiHs.369998

Genome annotation databases

EnsembliENST00000315005; ENSP00000323663; ENSG00000161692 [Q8NFT6-1]
ENST00000393547; ENSP00000377178; ENSG00000161692 [Q8NFT6-2]
GeneIDi80174
KEGGihsa:80174
UCSCiuc002ihf.4 human [Q8NFT6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDBF4B_HUMAN
AccessioniPrimary (citable) accession number: Q8NFT6
Secondary accession number(s): D3DX56
, Q8TEX0, Q96B19, Q9H912
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome