Q8NFT2 (STEA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metalloreductase STEAP2
EC=1.16.1.-

Alternative name(s):
Prostate cancer-associated protein 1
Protein up-regulated in metastatic prostate cancer
Short name=PUMPCn
Six-transmembrane epithelial antigen of prostate 2
SixTransMembrane protein of prostate 1

Gene names

Name:	STEAP2
Synonyms:	PCANAP1, STAMP1
ORF Names:	UNQ6507/PRO23203

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. Uses NAD+ as acceptor By similarity.
Cofactor	FAD By similarity.
Subcellular location	Endosome membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Expressed at high levels in prostate and at significantly lower levels in heart, brain, kidney, pancreas, and ovary. Ref.1 Ref.2 Ref.8
Sequence similarities	Belongs to the STEAP family. Contains 1 ferric oxidoreductase domain.

Ontologies

Keywords
Biological process	Electron transport Ion transport Iron transport Transport
Cellular component	Cell membrane Endosome Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Copper FAD Flavoprotein Iron Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane transport Inferred from direct assay Ref.1. Source: UniProtKB electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW endocytosis Inferred from direct assay Ref.1. Source: UniProtKB ion transport Inferred from electronic annotation. Source: UniProtKB-KW iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW regulated secretory pathway Inferred from direct assay Ref.1. Source: UniProtKB response to hormone stimulus Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB early endosome Inferred from direct assay Ref.1. Source: UniProtKB endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB trans-Golgi network transport vesicle Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NFT2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NFT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     445-490: GKIILFLPCISRKLKRIKKGWEKSQFLEEGMGGTIPHVSPERVTVM → DLLQLCRYPD

Isoform 3 (identifier: Q8NFT2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     396-490: STLGYVALLI...HVSPERVTVM → IFCSFADTQTELELEFVFLLTLLL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Metalloreductase STEAP2		PRO_0000191697
Regions
	Transmembrane	208 – 228	21	Helical; Potential
	Transmembrane	259 – 279	21	Helical; Potential
	Transmembrane	305 – 325	21	Helical; Potential
	Transmembrane	359 – 379	21	Helical; Potential
	Transmembrane	393 – 413	21	Helical; Potential
	Transmembrane	432 – 452	21	Helical; Potential
	Domain	259 – 407	149	Ferric oxidoreductase
Sites
	Metal binding	316	1	Iron (heme axial ligand) By similarity
	Metal binding	409	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	396 – 490	95	STLGY…RVTVM → IFCSFADTQTELELEFVFLL TLLL in isoform 3.		VSP_045590
	Alternative sequence	445 – 490	46	GKIIL…RVTVM → DLLQLCRYPD in isoform 2.		VSP_030999
	Natural variant	17	1	F → C. Ref.1 Ref.2 Ref.3 Ref.4 Ref.9 Corresponds to variant rs194520 [ dbSNP | Ensembl ].		VAR_060387
	Natural variant	40	1	D → Y. Corresponds to variant rs17863046 [ dbSNP | Ensembl ].		VAR_060388
	Natural variant	214	1	G → E. Corresponds to variant rs13228098 [ dbSNP | Ensembl ].		VAR_057727
	Natural variant	456	1	R → Q. Ref.2 Corresponds to variant rs194524 [ dbSNP | Ensembl ].		VAR_057728
	Natural variant	475	1	M → I. Ref.1 Ref.2 Ref.6 Ref.7 Corresponds to variant rs194525 [ dbSNP | Ensembl ].		VAR_060389
Experimental info
	Sequence conflict	17	1	F → V in AAN04080. Ref.2
	Sequence conflict	211	1	L → F in AAG32148. Ref.1
	Sequence conflict	211	1	L → F in AAG32149. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: DB12E252DE3F4CAF	FASTA	490	56,056
	10 20 30 40 50 60 MESISMMGSP KSLSETFLPN GINGIKDARK VTVGVIGSGD FAKSLTIRLI RCGYHVVIGS 70 80 90 100 110 120 RNPKFASEFF PHVVDVTHHE DALTKTNIIF VAIHREHYTS LWDLRHLLVG KILIDVSNNM 130 140 150 160 170 180 RINQYPESNA EYLASLFPDS LIVKGFNVVS AWALQLGPKD ASRQVYICSN NIQARQQVIE 190 200 210 220 230 240 LARQLNFIPI DLGSLSSARE IENLPLRLFT LWRGPVVVAI SLATFFFLYS FVRDVIHPYA 250 260 270 280 290 300 RNQQSDFYKI PIEIVNKTLP IVAITLLSLV YLAGLLAAAY QLYYGTKYRR FPPWLETWLQ 310 320 330 340 350 360 CRKQLGLLSF FFAMVHVAYS LCLPMRRSER YLFLNMAYQQ VHANIENSWN EEEVWRIEMY 370 380 390 400 410 420 ISFGIMSLGL LSLLAVTSIP SVSNALNWRE FSFIQSTLGY VALLISTFHV LIYGWKRAFE 430 440 450 460 470 480 EEYYRFYTPP NFVLALVLPS IVILGKIILF LPCISRKLKR IKKGWEKSQF LEEGMGGTIP 490 HVSPERVTVM
	Isoform 2 [UniParc]. Checksum: 1471E787D19E3E21	FASTA	454	52,052
	10 20 30 40 50 60 MESISMMGSP KSLSETFLPN GINGIKDARK VTVGVIGSGD FAKSLTIRLI RCGYHVVIGS 70 80 90 100 110 120 RNPKFASEFF PHVVDVTHHE DALTKTNIIF VAIHREHYTS LWDLRHLLVG KILIDVSNNM 130 140 150 160 170 180 RINQYPESNA EYLASLFPDS LIVKGFNVVS AWALQLGPKD ASRQVYICSN NIQARQQVIE 190 200 210 220 230 240 LARQLNFIPI DLGSLSSARE IENLPLRLFT LWRGPVVVAI SLATFFFLYS FVRDVIHPYA 250 260 270 280 290 300 RNQQSDFYKI PIEIVNKTLP IVAITLLSLV YLAGLLAAAY QLYYGTKYRR FPPWLETWLQ 310 320 330 340 350 360 CRKQLGLLSF FFAMVHVAYS LCLPMRRSER YLFLNMAYQQ VHANIENSWN EEEVWRIEMY 370 380 390 400 410 420 ISFGIMSLGL LSLLAVTSIP SVSNALNWRE FSFIQSTLGY VALLISTFHV LIYGWKRAFE 430 440 450 EEYYRFYTPP NFVLALVLPS IVILDLLQLC RYPD
	Isoform 3 [UniParc]. Checksum: 97C4B47BD22DA168	FASTA	419	47,916
	10 20 30 40 50 60 MESISMMGSP KSLSETFLPN GINGIKDARK VTVGVIGSGD FAKSLTIRLI RCGYHVVIGS 70 80 90 100 110 120 RNPKFASEFF PHVVDVTHHE DALTKTNIIF VAIHREHYTS LWDLRHLLVG KILIDVSNNM 130 140 150 160 170 180 RINQYPESNA EYLASLFPDS LIVKGFNVVS AWALQLGPKD ASRQVYICSN NIQARQQVIE 190 200 210 220 230 240 LARQLNFIPI DLGSLSSARE IENLPLRLFT LWRGPVVVAI SLATFFFLYS FVRDVIHPYA 250 260 270 280 290 300 RNQQSDFYKI PIEIVNKTLP IVAITLLSLV YLAGLLAAAY QLYYGTKYRR FPPWLETWLQ 310 320 330 340 350 360 CRKQLGLLSF FFAMVHVAYS LCLPMRRSER YLFLNMAYQQ VHANIENSWN EEEVWRIEMY 370 380 390 400 410 ISFGIMSLGL LSLLAVTSIP SVSNALNWRE FSFIQIFCSF ADTQTELELE FVFLLTLLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of STAMP1, a highly prostate specific six-trans-membrane protein that is overexpressed in prostate cancer." Korkmaz K.S., Elbi C.C., Korkmaz C.G., Loda M., Hager G.L., Saatcioglu F. J. Biol. Chem. 277:36689-36696(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, VARIANTS CYS-17 AND ILE-475. Tissue: Prostate.
[2]	"Cloning and characterization of a novel six-transmembrane protein STEAP2, expressed in normal and malignant prostate." Porkka K.P., Helenius M.A., Visakorpi T. Lab. Invest. 82:1573-1582(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, VARIANTS CYS-17; GLN-456 AND ILE-475. Tissue: Prostate.
[3]	"Bioinformatics identification and clinical validation of tumor antigens by analysis of EST and tissue microarray data." Zhang Z., Eberhard D.A., Polakis P., Grimaldi C., Frantz G.D., Hillan K.J., Wood W.I. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT CYS-17.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT CYS-17.
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-475.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-475.
[8]	"The Steap proteins are metalloreductases." Ohgami R.S., Campagna D.R., McDonald A., Fleming M.D. Blood 108:1388-1394(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-17, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY008444 mRNA. Translation: AAG32148.1. AY008445 mRNA. Translation: AAG32149.1. AF455138 mRNA. Translation: AAN04080.1. AF526382 mRNA. Translation: AAQ08976.1. AY358267 mRNA. Translation: AAQ88634.1. AC002064 Genomic DNA. No translation available. AC004969 Genomic DNA. No translation available. CH236949 Genomic DNA. Translation: EAL24165.1. CH471091 Genomic DNA. Translation: EAW76890.1. CH471091 Genomic DNA. Translation: EAW76894.1.
IPI	IPI00168894. IPI00434815. IPI00885092.
RefSeq	NP_001035755.1. NM_001040665.1. NP_001035756.1. NM_001040666.1. NP_001231873.1. NM_001244944.1. NP_001231875.1. NM_001244946.1. NP_694544.2. NM_152999.3.
UniGene	Hs.489051.
3D structure databases
ProteinModelPortal	Q8NFT2.
SMR	Q8NFT2. Positions 33-209.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000287908.
PTM databases
PhosphoSite	Q8NFT2.
Polymorphism databases
DMDM	296452950.
Proteomic databases
PaxDb	Q8NFT2.
PeptideAtlas	Q8NFT2.
PRIDE	Q8NFT2.
Protocols and materials databases
DNASU	261729.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000287908; ENSP00000287908; ENSG00000157214. ENST00000394621; ENSP00000378119; ENSG00000157214. ENST00000394622; ENSP00000378120; ENSG00000157214. ENST00000394624; ENSP00000378121; ENSG00000157214. ENST00000394626; ENSP00000378123; ENSG00000157214. ENST00000394629; ENSP00000378125; ENSG00000157214. ENST00000394632; ENSP00000378128; ENSG00000157214.
GeneID	261729.
KEGG	hsa:261729.
UCSC	uc003ujy.2. human.
Organism-specific databases
CTD	261729.
GeneCards	GC07P089796.
HGNC	HGNC:17885. STEAP2.
HPA	HPA029115.
MIM	605094. gene.
neXtProt	NX_Q8NFT2.
PharmGKB	PA38473.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2085.
HOGENOM	HOG000234491.
HOVERGEN	HBG054379.
InParanoid	Q8NFT2.
KO	K14738.
OMA	VSNNMRV.
PhylomeDB	Q8NFT2.
Gene expression databases
ArrayExpress	Q8NFT2.
Bgee	Q8NFT2.
CleanEx	HS_STEAP2.
Genevestigator	Q8NFT2.
GermOnline	ENSG00000157214. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013130. Fe3_Rdtase_TM_dom. IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. [Graphical view]
Pfam	PF03807. F420_oxidored. 1 hit. PF01794. Ferric_reduct. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	STEAP2. human.
GenomeRNAi	261729.
NextBio	93228.
SOURCE	Search...

Entry information

Entry name

STEA2_HUMAN

Accession

Primary (citable) accession number: Q8NFT2
Secondary accession number(s): A4D1F1 , G5E9C6, Q6UXN6, Q6YPB1, Q8IUE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.