Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8NFM4 (ADCY4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylate cyclase type 4

EC=4.6.1.1
Alternative name(s):
ATP pyrophosphate-lyase 4
Adenylate cyclase type IV
Adenylyl cyclase 4
Gene names
Name:ADCY4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1077 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a membrane-bound, calmodulin-insensitive adenylyl cyclase By similarity.

Catalytic activity

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactor

Binds 2 magnesium ions per subunit By similarity.

Enzyme regulation

Insensitive to calcium/calmodulin. Stimulated by the G protein beta and gamma subunit complex By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 2 guanylate cyclase domains.

Sequence caution

The sequence BAB71270.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processcAMP biosynthesis
   Cellular componentMembrane
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLyase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of phospholipase C activity

Traceable author statement. Source: Reactome

activation of protein kinase A activity

Traceable author statement. Source: Reactome

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Traceable author statement. Source: Reactome

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Traceable author statement. Source: Reactome

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

cAMP biosynthetic process

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular response to glucagon stimulus

Traceable author statement. Source: Reactome

energy reserve metabolic process

Traceable author statement. Source: Reactome

epidermal growth factor receptor signaling pathway

Traceable author statement. Source: Reactome

fibroblast growth factor receptor signaling pathway

Traceable author statement. Source: Reactome

innate immune response

Traceable author statement. Source: Reactome

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

neurotrophin TRK receptor signaling pathway

Traceable author statement. Source: Reactome

signal transduction

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

synaptic transmission

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

water transport

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 11549699. Source: UniProtKB

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

adenylate cyclase activity

Inferred from sequence or structural similarity. Source: BHF-UCL

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 17081159. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10771077Adenylate cyclase type 4
PRO_0000195690

Regions

Topological domain1 – 2828Cytoplasmic Potential
Transmembrane29 – 5022Helical; Potential
Transmembrane61 – 8020Helical; Potential
Transmembrane94 – 11724Helical; Potential
Transmembrane120 – 13819Helical; Potential
Transmembrane141 – 16222Helical; Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 585395Cytoplasmic Potential
Transmembrane586 – 60722Helical; Potential
Transmembrane611 – 63323Helical; Potential
Transmembrane664 – 68724Helical; Potential
Topological domain688 – 71427Extracellular Potential
Transmembrane715 – 73622Helical; Potential
Transmembrane744 – 76421Helical; Potential
Transmembrane791 – 80717Helical; Potential
Topological domain808 – 1077270Cytoplasmic Potential

Sites

Metal binding2781Magnesium 1 By similarity
Metal binding2781Magnesium 2 By similarity
Metal binding2791Magnesium 2; via carbonyl oxygen By similarity
Metal binding3221Magnesium 1 By similarity
Metal binding3221Magnesium 2 By similarity

Amino acid modifications

Glycosylation6971N-linked (GlcNAc...) Potential
Glycosylation7041N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5711E → G in BAB71270. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8NFM4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 24C0D5AE495CFD50

FASTA1,077119,794
        10         20         30         40         50         60 
MARLFSPRPP PSEDLFYETY YSLSQQYPLL LLLLGIVLCA LAALLAVAWA SGRELTSDPS 

        70         80         90        100        110        120 
FLTTVLCALG GFSLLLGLAS REQRLQRWTR PLSGLVWVAL LALGHAFLFT GGVVSAWDQV 

       130        140        150        160        170        180 
SYFLFVIFTA YAMLPLGMRD AAVAGLASSL SHLLVLGLYL GPQPDSRPAL LPQLAANAVL 

       190        200        210        220        230        240 
FLCGNVAGVY HKALMERALR ATFREALSSL HSRRRLDTEK KHQEHLLLSI LPAYLAREMK 

       250        260        270        280        290        300 
AEIMARLQAG QGSRPESTNN FHSLYVKRHQ GVSVLYADIV GFTRLASECS PKELVLMLNE 

       310        320        330        340        350        360 
LFGKFDQIAK EHECMRIKIL GDCYYCVSGL PLSLPDHAIN CVRMGLDMCR AIRKLRAATG 

       370        380        390        400        410        420 
VDINMRVGVH SGSVLCGVIG LQKWQYDVWS HDVTLANHME AGGVPGRVHI TGATLALLAG 

       430        440        450        460        470        480 
AYAVEDAGME HRDPYLRELG EPTYLVIDPR AEEEDEKGTA GGLLSSLEGL KMRPSLLMTR 

       490        500        510        520        530        540 
YLESWGAAKP FAHLSHGDSP VSTSTPLPEK TLASFSTQWS LDRSRTPRGL DDELDTGDAK 

       550        560        570        580        590        600 
FFQVIEQLNS QKQWKQSKDF NPLTLYFREK EMEKEYRLSA IPAFKYYEAC TFLVFLSNFI 

       610        620        630        640        650        660 
IQMLVTNRPP ALAITYSITF LLFLLILFVC FSEDLMRCVL KGPKMLHWLP ALSGLVATRP 

       670        680        690        700        710        720 
GLRIALGTAT ILLVFAMAIT SLFFFPTSSD CPFQAPNVSS MISNLSWELP GSLPLISVPY 

       730        740        750        760        770        780 
SMHCCTLGFL SCSLFLHMSF ELKLLLLLLW LAASCSLFLH SHAWLSECLI VRLYLGPLDS 

       790        800        810        820        830        840 
RPGVLKEPKL MGAISFFIFF FTLLVLARQN EYYCRLDFLW KKKLRQEREE TETMENLTRL 

       850        860        870        880        890        900 
LLENVLPAHV APQFIGQNRR NEDLYHQSYE CVCVLFASVP DFKEFYSESN INHEGLECLR 

       910        920        930        940        950        960 
LLNEIIADFD ELLSKPKFSG VEKIKTIGST YMAATGLNAT SGQDAQQDAE RSCSHLGTMV 

       970        980        990       1000       1010       1020 
EFAVALGSKL DVINKHSFNN FRLRVGLNHG PVVAGVIGAQ KPQYDIWGNT VNVASRMEST 

      1030       1040       1050       1060       1070 
GVLGKIQVTE ETAWALQSLG YTCYSRGVIK VKGKGQLCTY FLNTDLTRTG PPSATLG 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the human adenylyl cyclase gene family: cDNA, gene structure, and tissue distribution of the nine isoforms."
Ludwig M.G., Seuwen K.
J. Recept. Signal Transduct. 22:79-110(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta and Tongue.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF497516 mRNA. Translation: AAM94373.1.
AK056745 mRNA. Translation: BAB71270.1. Different initiation.
AK122714 mRNA. Translation: BAG53686.1.
CH471078 Genomic DNA. Translation: EAW66023.1.
CH471078 Genomic DNA. Translation: EAW66026.1.
CH471078 Genomic DNA. Translation: EAW66027.1.
BC117473 mRNA. Translation: AAI17474.1.
BC117475 mRNA. Translation: AAI17476.1.
CCDSCCDS9627.1.
RefSeqNP_001185497.1. NM_001198568.1.
NP_001185521.1. NM_001198592.1.
NP_640340.2. NM_139247.3.
UniGeneHs.443428.

3D structure databases

ProteinModelPortalQ8NFM4.
SMRQ8NFM4. Positions 261-447, 864-1043.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128229. 1 interaction.
IntActQ8NFM4. 4 interactions.
STRING9606.ENSP00000312126.

Chemistry

BindingDBQ8NFM4.
ChEMBLCHEMBL2097167.

PTM databases

PhosphoSiteQ8NFM4.

Polymorphism databases

DMDM25008336.

Proteomic databases

PaxDbQ8NFM4.
PRIDEQ8NFM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310677; ENSP00000312126; ENSG00000129467.
ENST00000418030; ENSP00000393177; ENSG00000129467.
ENST00000554068; ENSP00000452250; ENSG00000129467.
GeneID196883.
KEGGhsa:196883.
UCSCuc001wow.3. human.

Organism-specific databases

CTD196883.
GeneCardsGC14M024787.
HGNCHGNC:235. ADCY4.
HPACAB005070.
MIM600292. gene.
neXtProtNX_Q8NFM4.
PharmGKBPA24562.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2114.
HOVERGENHBG050458.
InParanoidQ8NFM4.
KOK08044.
OMAFLHSHAW.
OrthoDBEOG76X5ZC.
PhylomeDBQ8NFM4.
TreeFamTF313845.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_116125. Disease.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ8NFM4.
BgeeQ8NFM4.
CleanExHS_ADCY4.
GenevestigatorQ8NFM4.

Family and domain databases

Gene3D3.30.70.1230. 2 hits.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR009398. Adenylate_cyclase-like.
[Graphical view]
PfamPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
SMARTSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMSSF55073. SSF55073. 2 hits.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiADCY4.
GenomeRNAi196883.
NextBio89574.
PROQ8NFM4.
SOURCESearch...

Entry information

Entry nameADCY4_HUMAN
AccessionPrimary (citable) accession number: Q8NFM4
Secondary accession number(s): B3KV74 expand/collapse secondary AC list , D3DS75, Q17R40, Q96ML7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM