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Protein

Retinoic acid-induced protein 3

Gene

GPRC5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed:18000218).By similarity1 Publication

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • G-protein coupled receptor activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoic acid-induced protein 3
Alternative name(s):
G-protein coupled receptor family C group 5 member A
Phorbol ester induced gene 11 Publication
Short name:
PEIG-1
Retinoic acid-induced gene 1 protein
Short name:
RAIG-1
Gene namesi
Name:GPRC5A
Synonyms:GPCR5A, RAI3, RAIG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000013588.5
HGNCiHGNC:9836 GPRC5A
MIMi604138 gene
neXtProtiNX_Q8NFJ5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33ExtracellularCuratedAdd BLAST33
Transmembranei34 – 54Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini55 – 68CytoplasmicCuratedAdd BLAST14
Transmembranei69 – 89Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini90 – 97ExtracellularCurated8
Transmembranei98 – 118Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini119 – 129CytoplasmicCuratedAdd BLAST11
Transmembranei130 – 150Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini151 – 176ExtracellularCuratedAdd BLAST26
Transmembranei177 – 197Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini198 – 212CytoplasmicCuratedAdd BLAST15
Transmembranei213 – 233Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini234 – 247ExtracellularCuratedAdd BLAST14
Transmembranei248 – 268Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini269 – 357CytoplasmicCuratedAdd BLAST89

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi9052
OpenTargetsiENSG00000013588
PharmGKBiPA34194

Chemistry databases

DrugBankiDB00755 Tretinoin

Polymorphism and mutation databases

BioMutaiGPRC5A
DMDMi46396943

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002068951 – 357Retinoic acid-induced protein 3Add BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei301PhosphoserineCombined sources1
Modified residuei317Phosphotyrosine1 Publication1
Modified residuei320Phosphotyrosine1 Publication1
Modified residuei345PhosphoserineCombined sources1
Modified residuei347PhosphotyrosineCombined sources1 Publication1
Modified residuei350Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated in two conserved double-tyrosine motifs, TYR-317/TYR-320 and TYR-347/TYR-350, by EGFR; leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. TYR-317 and TYR-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8NFJ5
MaxQBiQ8NFJ5
PaxDbiQ8NFJ5
PeptideAtlasiQ8NFJ5
PRIDEiQ8NFJ5

PTM databases

iPTMnetiQ8NFJ5
PhosphoSitePlusiQ8NFJ5
SwissPalmiQ8NFJ5

Expressioni

Tissue specificityi

Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers (PubMed:9857033, PubMed:18000218). Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart.4 Publications

Inductioni

By all-trans retinoic acid (ATRA).1 Publication

Gene expression databases

BgeeiENSG00000013588
CleanExiHS_GPRC5A
ExpressionAtlasiQ8NFJ5 baseline and differential
GenevisibleiQ8NFJ5 HS

Organism-specific databases

HPAiHPA007928
HPA046526

Interactioni

Subunit structurei

Interacts (via its transmembrane domain) with EGFR.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114514, 37 interactors
IntActiQ8NFJ5, 30 interactors
STRINGi9606.ENSP00000014914

Structurei

3D structure databases

ProteinModelPortaliQ8NFJ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIGU Eukaryota
ENOG410YD51 LUCA
GeneTreeiENSGT00520000055551
HOGENOMiHOG000116197
HOVERGENiHBG049967
InParanoidiQ8NFJ5
KOiK08468
OMAiWDDTILS
OrthoDBiEOG091G0DAG
PhylomeDBiQ8NFJ5
TreeFamiTF321410

Family and domain databases

InterProiView protein in InterPro
IPR017978 GPCR_3_C
PfamiView protein in Pfam
PF00003 7tm_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8NFJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP
60 70 80 90 100
ILVCKVQDSN RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL
110 120 130 140 150
FGILFSICFS CLLAHAVSLT KLVRGRKPLS LLVILGLAVG FSLVQDVIAI
160 170 180 190 200
EYIVLTMNRT NVNVFSELSA PRRNEDFVLL LTYVLFLMAL TFLMSSFTFC
210 220 230 240 250
GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW DDTILSSALA
260 270 280 290 300
ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY
310 320 330 340 350
SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY

EVKKEGS
Length:357
Mass (Da):40,251
Last modified:April 13, 2004 - v2
Checksum:i7BEB524BF6F307E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti292S → G in AAM77594 (PubMed:8832110).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0492819C → F. Corresponds to variant dbSNP:rs11550683Ensembl.1
Natural variantiVAR_018296118S → G. Corresponds to variant dbSNP:rs850932Ensembl.1
Natural variantiVAR_049282182T → A. Corresponds to variant dbSNP:rs12368599Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506289 mRNA Translation: AAM77594.1
AF095448 mRNA Translation: AAC98506.1
AK001761 mRNA Translation: BAA91890.1
AK122672 mRNA Translation: BAG53657.1
CH471094 Genomic DNA Translation: EAW96289.1
BC003665 mRNA Translation: AAH03665.1
CCDSiCCDS8657.1
RefSeqiNP_003970.1, NM_003979.3
UniGeneiHs.631733

Genome annotation databases

EnsembliENST00000014914; ENSP00000014914; ENSG00000013588
GeneIDi9052
KEGGihsa:9052
UCSCiuc001rba.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRAI3_HUMAN
AccessioniPrimary (citable) accession number: Q8NFJ5
Secondary accession number(s): B3KV45, O95357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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