Q8NFJ5 (RAI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinoic acid-induced protein 3

Alternative name(s):
G-protein coupled receptor family C group 5 member A
Orphan G-protein-coupling receptor PEIG-1
Retinoic acid-induced gene 1 protein
Short name=RAIG-1

Gene names

Name:	GPRC5A
Synonyms:	GPCR5A, RAI3, RAIG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Localized in the plasma membrane and perinuclear vesicles.
Tissue specificity	Expressed at high level in fetal and adult lung tissues. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to Ref.6, expressed at low but detectable level in pancreas and heart. Ref.6 Ref.7
Induction	By all-trans retinoic acid (ATRA).
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Retinoic acid-induced protein 3		PRO_0000206895
Regions
	Topological domain	1 – 33	33	Extracellular Potential
	Transmembrane	34 – 54	21	Helical; Name=1; Potential
	Topological domain	55 – 68	14	Cytoplasmic Potential
	Transmembrane	69 – 89	21	Helical; Name=2; Potential
	Topological domain	90 – 97	8	Extracellular Potential
	Transmembrane	98 – 118	21	Helical; Name=3; Potential
	Topological domain	119 – 129	11	Cytoplasmic Potential
	Transmembrane	130 – 150	21	Helical; Name=4; Potential
	Topological domain	151 – 176	26	Extracellular Potential
	Transmembrane	177 – 197	21	Helical; Name=5; Potential
	Topological domain	198 – 212	15	Cytoplasmic Potential
	Transmembrane	213 – 233	21	Helical; Name=6; Potential
	Topological domain	234 – 247	14	Extracellular Potential
	Transmembrane	248 – 268	21	Helical; Name=7; Potential
	Topological domain	269 – 357	89	Cytoplasmic Potential
Amino acid modifications
	Modified residue	301	1	Phosphoserine Ref.11
	Modified residue	345	1	Phosphoserine Ref.10 Ref.11
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	9	1	C → F. Corresponds to variant rs11550683 [ dbSNP | Ensembl ].		VAR_049281
	Natural variant	118	1	S → G. Corresponds to variant rs850932 [ dbSNP | Ensembl ].		VAR_018296
	Natural variant	182	1	T → A. Corresponds to variant rs12368599 [ dbSNP | Ensembl ].		VAR_049282
Experimental info
	Sequence conflict	292	1	S → G in AAM77594. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8NFJ5 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 7BEB524BF6F307E5	FASTA	357	40,251
	10 20 30 40 50 60 MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP ILVCKVQDSN 70 80 90 100 110 120 RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL FGILFSICFS CLLAHAVSLT 130 140 150 160 170 180 KLVRGRKPLS LLVILGLAVG FSLVQDVIAI EYIVLTMNRT NVNVFSELSA PRRNEDFVLL 190 200 210 220 230 240 LTYVLFLMAL TFLMSSFTFC GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW 250 260 270 280 290 300 DDTILSSALA ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY 310 320 330 340 350 SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY EVKKEGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor." Cheng Y., Lotan R. J. Biol. Chem. 273:35008-35015(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	Cafferata E.G., Gonzalez-Guerrico A.M., Costanzo R., Pivetta O.H., Santa-Coloma T.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon carcinoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[6]	"Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain." Braeuner-Osborne H., Krogsgaard-Larsen P. Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)." Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N. Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-345, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF095448 mRNA. Translation: AAC98506.1. AF506289 mRNA. Translation: AAM77594.1. AK001761 mRNA. Translation: BAA91890.1. AK122672 mRNA. Translation: BAG53657.1. CH471094 Genomic DNA. Translation: EAW96289.1. BC003665 mRNA. Translation: AAH03665.1.
IPI	IPI00022624.
RefSeq	NP_003970.1. NM_003979.3.
UniGene	Hs.631733.
3D structure databases
ProteinModelPortal	Q8NFJ5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NFJ5. 1 interaction.
MINT	MINT-5005465.
STRING	9606.ENSP00000014914.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q8NFJ5.
Polymorphism databases
DMDM	46396943.
Proteomic databases
PaxDb	Q8NFJ5.
PeptideAtlas	Q8NFJ5.
PRIDE	Q8NFJ5.
Protocols and materials databases
DNASU	9052.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000014914; ENSP00000014914; ENSG00000013588.
GeneID	9052.
KEGG	hsa:9052.
UCSC	uc001rba.3. human.
Organism-specific databases
CTD	9052.
GeneCards	GC12P013047.
HGNC	HGNC:9836. GPRC5A.
HPA	CAB020771. HPA007928.
MIM	604138. gene.
neXtProt	NX_Q8NFJ5.
PharmGKB	PA34194.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44023.
HOGENOM	HOG000116197.
HOVERGEN	HBG049967.
InParanoid	Q8NFJ5.
KO	K08468.
OMA	WDDTILS.
OrthoDB	EOG4NS3BT.
PhylomeDB	Q8NFJ5.
Gene expression databases
ArrayExpress	Q8NFJ5.
Bgee	Q8NFJ5.
CleanEx	HS_GPRC5A.
Genevestigator	Q8NFJ5.
GermOnline	ENSG00000013588. Homo sapiens.
Family and domain databases
InterPro	IPR017978. GPCR_3_C. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. [Graphical view]
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GPRC5A. human.
DrugBank	DB00755. Tretinoin.
GenomeRNAi	9052.
NextBio	33915.
SOURCE	Search...

Entry information

Entry name

RAI3_HUMAN

Accession

Primary (citable) accession number: Q8NFJ5
Secondary accession number(s): B3KV45, O95357

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.