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Q8NFJ5

- RAI3_HUMAN

UniProt

Q8NFJ5 - RAI3_HUMAN

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Protein

Retinoic acid-induced protein 3

Gene

GPRC5A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: ProtInc

GO - Biological processi

  1. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoic acid-induced protein 3
Alternative name(s):
G-protein coupled receptor family C group 5 member A
Orphan G-protein-coupling receptor PEIG-1
Retinoic acid-induced gene 1 protein
Short name:
RAIG-1
Gene namesi
Name:GPRC5A
Synonyms:GPCR5A, RAI3, RAIG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:9836. GPRC5A.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein
Note: Localized in the plasma membrane and perinuclear vesicles.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularSequence AnalysisAdd
BLAST
Transmembranei34 – 5421Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini55 – 6814CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei69 – 8921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini90 – 978ExtracellularSequence Analysis
Transmembranei98 – 11821Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini119 – 12911CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei130 – 15021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini151 – 17626ExtracellularSequence AnalysisAdd
BLAST
Transmembranei177 – 19721Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini198 – 21215CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei213 – 23321Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini234 – 24714ExtracellularSequence AnalysisAdd
BLAST
Transmembranei248 – 26821Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini269 – 35789CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: UniProtKB
  3. integral component of plasma membrane Source: ProtInc
  4. intracellular membrane-bounded organelle Source: HPA
  5. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34194.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Retinoic acid-induced protein 3PRO_0000206895Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
Modified residuei301 – 3011Phosphoserine1 Publication
Modified residuei345 – 3451Phosphoserine2 Publications
Modified residuei350 – 3501PhosphotyrosineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8NFJ5.
PaxDbiQ8NFJ5.
PeptideAtlasiQ8NFJ5.
PRIDEiQ8NFJ5.

PTM databases

PhosphoSiteiQ8NFJ5.

Expressioni

Tissue specificityi

Expressed at high level in fetal and adult lung tissues. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart.2 Publications

Inductioni

By all-trans retinoic acid (ATRA).

Gene expression databases

BgeeiQ8NFJ5.
CleanExiHS_GPRC5A.
ExpressionAtlasiQ8NFJ5. baseline and differential.
GenevestigatoriQ8NFJ5.

Organism-specific databases

HPAiCAB020771.
HPA007928.
HPA046526.

Interactioni

Protein-protein interaction databases

BioGridi114514. 8 interactions.
IntActiQ8NFJ5. 1 interaction.
MINTiMINT-5005465.
STRINGi9606.ENSP00000014914.

Structurei

3D structure databases

ProteinModelPortaliQ8NFJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44023.
GeneTreeiENSGT00520000055551.
HOGENOMiHOG000116197.
HOVERGENiHBG049967.
InParanoidiQ8NFJ5.
KOiK08468.
OMAiTMNRTNV.
OrthoDBiEOG7RRF79.
PhylomeDBiQ8NFJ5.
TreeFamiTF321410.

Family and domain databases

InterProiIPR017978. GPCR_3_C.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NFJ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP
60 70 80 90 100
ILVCKVQDSN RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL
110 120 130 140 150
FGILFSICFS CLLAHAVSLT KLVRGRKPLS LLVILGLAVG FSLVQDVIAI
160 170 180 190 200
EYIVLTMNRT NVNVFSELSA PRRNEDFVLL LTYVLFLMAL TFLMSSFTFC
210 220 230 240 250
GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW DDTILSSALA
260 270 280 290 300
ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY
310 320 330 340 350
SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY

EVKKEGS
Length:357
Mass (Da):40,251
Last modified:April 13, 2004 - v2
Checksum:i7BEB524BF6F307E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti292 – 2921S → G in AAM77594. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91C → F.
Corresponds to variant rs11550683 [ dbSNP | Ensembl ].
VAR_049281
Natural varianti118 – 1181S → G.
Corresponds to variant rs850932 [ dbSNP | Ensembl ].
VAR_018296
Natural varianti182 – 1821T → A.
Corresponds to variant rs12368599 [ dbSNP | Ensembl ].
VAR_049282

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095448 mRNA. Translation: AAC98506.1.
AF506289 mRNA. Translation: AAM77594.1.
AK001761 mRNA. Translation: BAA91890.1.
AK122672 mRNA. Translation: BAG53657.1.
CH471094 Genomic DNA. Translation: EAW96289.1.
BC003665 mRNA. Translation: AAH03665.1.
CCDSiCCDS8657.1.
RefSeqiNP_003970.1. NM_003979.3.
UniGeneiHs.631733.

Genome annotation databases

EnsembliENST00000014914; ENSP00000014914; ENSG00000013588.
GeneIDi9052.
KEGGihsa:9052.
UCSCiuc001rba.3. human.

Polymorphism databases

DMDMi46396943.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095448 mRNA. Translation: AAC98506.1 .
AF506289 mRNA. Translation: AAM77594.1 .
AK001761 mRNA. Translation: BAA91890.1 .
AK122672 mRNA. Translation: BAG53657.1 .
CH471094 Genomic DNA. Translation: EAW96289.1 .
BC003665 mRNA. Translation: AAH03665.1 .
CCDSi CCDS8657.1.
RefSeqi NP_003970.1. NM_003979.3.
UniGenei Hs.631733.

3D structure databases

ProteinModelPortali Q8NFJ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114514. 8 interactions.
IntActi Q8NFJ5. 1 interaction.
MINTi MINT-5005465.
STRINGi 9606.ENSP00000014914.

Chemistry

DrugBanki DB00755. Tretinoin.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q8NFJ5.

Polymorphism databases

DMDMi 46396943.

Proteomic databases

MaxQBi Q8NFJ5.
PaxDbi Q8NFJ5.
PeptideAtlasi Q8NFJ5.
PRIDEi Q8NFJ5.

Protocols and materials databases

DNASUi 9052.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000014914 ; ENSP00000014914 ; ENSG00000013588 .
GeneIDi 9052.
KEGGi hsa:9052.
UCSCi uc001rba.3. human.

Organism-specific databases

CTDi 9052.
GeneCardsi GC12P013047.
HGNCi HGNC:9836. GPRC5A.
HPAi CAB020771.
HPA007928.
HPA046526.
MIMi 604138. gene.
neXtProti NX_Q8NFJ5.
PharmGKBi PA34194.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG44023.
GeneTreei ENSGT00520000055551.
HOGENOMi HOG000116197.
HOVERGENi HBG049967.
InParanoidi Q8NFJ5.
KOi K08468.
OMAi TMNRTNV.
OrthoDBi EOG7RRF79.
PhylomeDBi Q8NFJ5.
TreeFami TF321410.

Miscellaneous databases

ChiTaRSi GPRC5A. human.
GeneWikii GPRC5A.
GenomeRNAii 9052.
NextBioi 33915.
PROi Q8NFJ5.
SOURCEi Search...

Gene expression databases

Bgeei Q8NFJ5.
CleanExi HS_GPRC5A.
ExpressionAtlasi Q8NFJ5. baseline and differential.
Genevestigatori Q8NFJ5.

Family and domain databases

InterProi IPR017978. GPCR_3_C.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor."
    Cheng Y., Lotan R.
    J. Biol. Chem. 273:35008-35015(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. Cafferata E.G., Gonzalez-Guerrico A.M., Costanzo R., Pivetta O.H., Santa-Coloma T.A.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Colon carcinoma.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  6. "Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
    Braeuner-Osborne H., Krogsgaard-Larsen P.
    Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
    Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
    Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
    Yu L.R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
    J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAI3_HUMAN
AccessioniPrimary (citable) accession number: Q8NFJ5
Secondary accession number(s): B3KV45, O95357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3