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Q8NFJ5

- RAI3_HUMAN

UniProt

Q8NFJ5 - RAI3_HUMAN

Protein

Retinoic acid-induced protein 3

Gene

GPRC5A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: ProtInc

    GO - Biological processi

    1. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retinoic acid-induced protein 3
    Alternative name(s):
    G-protein coupled receptor family C group 5 member A
    Orphan G-protein-coupling receptor PEIG-1
    Retinoic acid-induced gene 1 protein
    Short name:
    RAIG-1
    Gene namesi
    Name:GPRC5A
    Synonyms:GPCR5A, RAI3, RAIG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:9836. GPRC5A.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein
    Note: Localized in the plasma membrane and perinuclear vesicles.

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. extracellular vesicular exosome Source: UniProt
    3. integral component of plasma membrane Source: ProtInc
    4. intracellular membrane-bounded organelle Source: HPA
    5. plasma membrane Source: HPA

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34194.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Retinoic acid-induced protein 3PRO_0000206895Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
    Modified residuei301 – 3011Phosphoserine1 Publication
    Modified residuei345 – 3451Phosphoserine2 Publications
    Modified residuei350 – 3501PhosphotyrosineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8NFJ5.
    PaxDbiQ8NFJ5.
    PeptideAtlasiQ8NFJ5.
    PRIDEiQ8NFJ5.

    PTM databases

    PhosphoSiteiQ8NFJ5.

    Expressioni

    Tissue specificityi

    Expressed at high level in fetal and adult lung tissues. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart.2 Publications

    Inductioni

    By all-trans retinoic acid (ATRA).

    Gene expression databases

    ArrayExpressiQ8NFJ5.
    BgeeiQ8NFJ5.
    CleanExiHS_GPRC5A.
    GenevestigatoriQ8NFJ5.

    Organism-specific databases

    HPAiCAB020771.
    HPA007928.
    HPA046526.

    Interactioni

    Protein-protein interaction databases

    BioGridi114514. 8 interactions.
    IntActiQ8NFJ5. 1 interaction.
    MINTiMINT-5005465.
    STRINGi9606.ENSP00000014914.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NFJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3333ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini55 – 6814CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini90 – 978ExtracellularSequence Analysis
    Topological domaini119 – 12911CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini151 – 17626ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini198 – 21215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini234 – 24714ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini269 – 35789CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 5421Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei177 – 19721Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei213 – 23321Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei248 – 26821Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44023.
    HOGENOMiHOG000116197.
    HOVERGENiHBG049967.
    InParanoidiQ8NFJ5.
    KOiK08468.
    OMAiTMNRTNV.
    OrthoDBiEOG7RRF79.
    PhylomeDBiQ8NFJ5.
    TreeFamiTF321410.

    Family and domain databases

    InterProiIPR017978. GPCR_3_C.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NFJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP    50
    ILVCKVQDSN RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL 100
    FGILFSICFS CLLAHAVSLT KLVRGRKPLS LLVILGLAVG FSLVQDVIAI 150
    EYIVLTMNRT NVNVFSELSA PRRNEDFVLL LTYVLFLMAL TFLMSSFTFC 200
    GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW DDTILSSALA 250
    ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY 300
    SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY 350
    EVKKEGS 357
    Length:357
    Mass (Da):40,251
    Last modified:April 13, 2004 - v2
    Checksum:i7BEB524BF6F307E5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti292 – 2921S → G in AAM77594. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91C → F.
    Corresponds to variant rs11550683 [ dbSNP | Ensembl ].
    VAR_049281
    Natural varianti118 – 1181S → G.
    Corresponds to variant rs850932 [ dbSNP | Ensembl ].
    VAR_018296
    Natural varianti182 – 1821T → A.
    Corresponds to variant rs12368599 [ dbSNP | Ensembl ].
    VAR_049282

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095448 mRNA. Translation: AAC98506.1.
    AF506289 mRNA. Translation: AAM77594.1.
    AK001761 mRNA. Translation: BAA91890.1.
    AK122672 mRNA. Translation: BAG53657.1.
    CH471094 Genomic DNA. Translation: EAW96289.1.
    BC003665 mRNA. Translation: AAH03665.1.
    CCDSiCCDS8657.1.
    RefSeqiNP_003970.1. NM_003979.3.
    UniGeneiHs.631733.

    Genome annotation databases

    EnsembliENST00000014914; ENSP00000014914; ENSG00000013588.
    GeneIDi9052.
    KEGGihsa:9052.
    UCSCiuc001rba.3. human.

    Polymorphism databases

    DMDMi46396943.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095448 mRNA. Translation: AAC98506.1 .
    AF506289 mRNA. Translation: AAM77594.1 .
    AK001761 mRNA. Translation: BAA91890.1 .
    AK122672 mRNA. Translation: BAG53657.1 .
    CH471094 Genomic DNA. Translation: EAW96289.1 .
    BC003665 mRNA. Translation: AAH03665.1 .
    CCDSi CCDS8657.1.
    RefSeqi NP_003970.1. NM_003979.3.
    UniGenei Hs.631733.

    3D structure databases

    ProteinModelPortali Q8NFJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114514. 8 interactions.
    IntActi Q8NFJ5. 1 interaction.
    MINTi MINT-5005465.
    STRINGi 9606.ENSP00000014914.

    Chemistry

    DrugBanki DB00755. Tretinoin.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q8NFJ5.

    Polymorphism databases

    DMDMi 46396943.

    Proteomic databases

    MaxQBi Q8NFJ5.
    PaxDbi Q8NFJ5.
    PeptideAtlasi Q8NFJ5.
    PRIDEi Q8NFJ5.

    Protocols and materials databases

    DNASUi 9052.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000014914 ; ENSP00000014914 ; ENSG00000013588 .
    GeneIDi 9052.
    KEGGi hsa:9052.
    UCSCi uc001rba.3. human.

    Organism-specific databases

    CTDi 9052.
    GeneCardsi GC12P013047.
    HGNCi HGNC:9836. GPRC5A.
    HPAi CAB020771.
    HPA007928.
    HPA046526.
    MIMi 604138. gene.
    neXtProti NX_Q8NFJ5.
    PharmGKBi PA34194.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44023.
    HOGENOMi HOG000116197.
    HOVERGENi HBG049967.
    InParanoidi Q8NFJ5.
    KOi K08468.
    OMAi TMNRTNV.
    OrthoDBi EOG7RRF79.
    PhylomeDBi Q8NFJ5.
    TreeFami TF321410.

    Miscellaneous databases

    ChiTaRSi GPRC5A. human.
    GeneWikii GPRC5A.
    GenomeRNAii 9052.
    NextBioi 33915.
    PROi Q8NFJ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8NFJ5.
    Bgeei Q8NFJ5.
    CleanExi HS_GPRC5A.
    Genevestigatori Q8NFJ5.

    Family and domain databases

    InterProi IPR017978. GPCR_3_C.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor."
      Cheng Y., Lotan R.
      J. Biol. Chem. 273:35008-35015(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. Cafferata E.G., Gonzalez-Guerrico A.M., Costanzo R., Pivetta O.H., Santa-Coloma T.A.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Colon carcinoma.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    6. "Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
      Braeuner-Osborne H., Krogsgaard-Larsen P.
      Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
      Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
      Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
      Yu L.R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
      J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAI3_HUMAN
    AccessioniPrimary (citable) accession number: Q8NFJ5
    Secondary accession number(s): B3KV45, O95357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3