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Q8NFJ5 (RAI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retinoic acid-induced protein 3
Alternative name(s):
G-protein coupled receptor family C group 5 member A
Orphan G-protein-coupling receptor PEIG-1
Retinoic acid-induced gene 1 protein
Short name=RAIG-1
Gene names
Name:GPRC5A
Synonyms:GPCR5A, RAI3, RAIG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Localized in the plasma membrane and perinuclear vesicles.

Tissue specificity

Expressed at high level in fetal and adult lung tissues. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to Ref.6, expressed at low but detectable level in pancreas and heart. Ref.6 Ref.7

Induction

By all-trans retinoic acid (ATRA).

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Retinoic acid-induced protein 3
PRO_0000206895

Regions

Topological domain1 – 3333Extracellular Potential
Transmembrane34 – 5421Helical; Name=1; Potential
Topological domain55 – 6814Cytoplasmic Potential
Transmembrane69 – 8921Helical; Name=2; Potential
Topological domain90 – 978Extracellular Potential
Transmembrane98 – 11821Helical; Name=3; Potential
Topological domain119 – 12911Cytoplasmic Potential
Transmembrane130 – 15021Helical; Name=4; Potential
Topological domain151 – 17626Extracellular Potential
Transmembrane177 – 19721Helical; Name=5; Potential
Topological domain198 – 21215Cytoplasmic Potential
Transmembrane213 – 23321Helical; Name=6; Potential
Topological domain234 – 24714Extracellular Potential
Transmembrane248 – 26821Helical; Name=7; Potential
Topological domain269 – 35789Cytoplasmic Potential

Amino acid modifications

Modified residue3011Phosphoserine Ref.11
Modified residue3451Phosphoserine Ref.10 Ref.11
Modified residue3501Phosphotyrosine By similarity
Glycosylation1581N-linked (GlcNAc...) Potential

Natural variations

Natural variant91C → F.
Corresponds to variant rs11550683 [ dbSNP | Ensembl ].
VAR_049281
Natural variant1181S → G.
Corresponds to variant rs850932 [ dbSNP | Ensembl ].
VAR_018296
Natural variant1821T → A.
Corresponds to variant rs12368599 [ dbSNP | Ensembl ].
VAR_049282

Experimental info

Sequence conflict2921S → G in AAM77594. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8NFJ5 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 7BEB524BF6F307E5

FASTA35740,251
        10         20         30         40         50         60 
MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP ILVCKVQDSN 

        70         80         90        100        110        120 
RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL FGILFSICFS CLLAHAVSLT 

       130        140        150        160        170        180 
KLVRGRKPLS LLVILGLAVG FSLVQDVIAI EYIVLTMNRT NVNVFSELSA PRRNEDFVLL 

       190        200        210        220        230        240 
LTYVLFLMAL TFLMSSFTFC GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW 

       250        260        270        280        290        300 
DDTILSSALA ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY 

       310        320        330        340        350 
SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY EVKKEGS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor."
Cheng Y., Lotan R.
J. Biol. Chem. 273:35008-35015(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]Cafferata E.G., Gonzalez-Guerrico A.M., Costanzo R., Pivetta O.H., Santa-Coloma T.A.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Colon carcinoma.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[6]"Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
Braeuner-Osborne H., Krogsgaard-Larsen P.
Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF095448 mRNA. Translation: AAC98506.1.
AF506289 mRNA. Translation: AAM77594.1.
AK001761 mRNA. Translation: BAA91890.1.
AK122672 mRNA. Translation: BAG53657.1.
CH471094 Genomic DNA. Translation: EAW96289.1.
BC003665 mRNA. Translation: AAH03665.1.
RefSeqNP_003970.1. NM_003979.3.
UniGeneHs.631733.

3D structure databases

ProteinModelPortalQ8NFJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114514. 8 interactions.
IntActQ8NFJ5. 1 interaction.
MINTMINT-5005465.
STRING9606.ENSP00000014914.

Chemistry

DrugBankDB00755. Tretinoin.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ8NFJ5.

Polymorphism databases

DMDM46396943.

Proteomic databases

PaxDbQ8NFJ5.
PeptideAtlasQ8NFJ5.
PRIDEQ8NFJ5.

Protocols and materials databases

DNASU9052.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000014914; ENSP00000014914; ENSG00000013588.
GeneID9052.
KEGGhsa:9052.
UCSCuc001rba.3. human.

Organism-specific databases

CTD9052.
GeneCardsGC12P013047.
HGNCHGNC:9836. GPRC5A.
HPACAB020771.
HPA007928.
HPA046526.
MIM604138. gene.
neXtProtNX_Q8NFJ5.
PharmGKBPA34194.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG44023.
HOGENOMHOG000116197.
HOVERGENHBG049967.
InParanoidQ8NFJ5.
KOK08468.
OMATMNRTNV.
OrthoDBEOG7RRF79.
PhylomeDBQ8NFJ5.
TreeFamTF321410.

Gene expression databases

ArrayExpressQ8NFJ5.
BgeeQ8NFJ5.
CleanExHS_GPRC5A.
GenevestigatorQ8NFJ5.

Family and domain databases

InterProIPR017978. GPCR_3_C.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPRC5A. human.
GeneWikiGPRC5A.
GenomeRNAi9052.
NextBio33915.
PROQ8NFJ5.
SOURCESearch...

Entry information

Entry nameRAI3_HUMAN
AccessionPrimary (citable) accession number: Q8NFJ5
Secondary accession number(s): B3KV45, O95357
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 16, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries