Q8NFI3 (ENASE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase
Short name=ENGase
EC=3.2.1.96

Gene names

Name:

ENGASE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol. Ref.1
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Cytoplasm › cytosol By similarity.
Tissue specificity	Widely expressed. Expressed at higher level in thymus and spleen. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 85 family. Contains 1 BRCT domain.
Sequence caution	The sequence AAH24213.2 differs from that shown. Reason: Frameshift at position 694.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Glycosidase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NFI3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NFI3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
     378-743: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NFI3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
     407-743: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Cytosolic endo-beta-N-acetylglucosaminidase		PRO_0000328867
Regions
	Domain	291 – 383	93	BRCT
Amino acid modifications
	Modified residue	66	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	347 – 406	60	SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3.		VSP_032835
	Alternative sequence	364 – 377	14	GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2.		VSP_032836
	Alternative sequence	378 – 743	366	Missing in isoform 2.		VSP_032837
	Alternative sequence	407 – 743	337	Missing in isoform 3.		VSP_032838
	Natural variant	596	1	S → N. Ref.5 Corresponds to variant rs4789879 [ dbSNP | Ensembl ].		VAR_060188
	Natural variant	731	1	E → K. Corresponds to variant rs11871357 [ dbSNP | Ensembl ].		VAR_060189
Experimental info
	Sequence conflict	152	1	F → L in BAB15158. Ref.2
	Sequence conflict	224	1	T → A in CAH56405. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 384DA533AE549FC5	FASTA	743	83,987
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL IRKHGFSVAL 370 380 390 400 410 420 FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF VTSFCLGMGA RRVCYGQEEA 430 440 450 460 470 480 VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR THCCLEDAWH GGSSLLVRGV IPPEVGNVAV 490 500 510 520 530 540 RLFSLQAPVP PKIYLSMVYK LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR 550 560 570 580 590 600 PLRVPPTKLA RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR 610 620 630 640 650 660 LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH WSFLLSQVRC 670 680 690 700 710 720 FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL LVEAAGPGQD RRMEFLVEPV 730 740 PKEGFRVPQA EWGRAVLLYS APA
	Isoform 2 [UniParc]. Checksum: A60D0AE20C2EDD79	FASTA	377	43,107
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL IRKHGFSVAL 370 FAPSCSVFPG VGNLLCC
	Isoform 3 [UniParc]. Checksum: 4EB91EC292C1A61A	FASTA	406	46,090
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKVGGG FRPRASGPVP 370 380 390 400 PLGPHFLMDL PFPSAPQRND SSCSSQSGDP VALRNRCPAP AKLCPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol." Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S., Inoue Y., Emori Y. Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hepatoma.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-680 (ISOFORM 3). Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 486-743 (ISOFORM 1), VARIANT ASN-596. Tissue: Skin.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF512564 mRNA. Translation: AAM80487.1. AK025518 mRNA. Translation: BAB15158.1. CH471099 Genomic DNA. Translation: EAW89562.1. AL110283 mRNA. Translation: CAH56405.1. BC024213 mRNA. Translation: AAH24213.2. Frameshift.
IPI	IPI00168838. IPI00874016. IPI00889689.
RefSeq	NP_001036038.1. NM_001042573.2.
UniGene	Hs.29288.
3D structure databases
ProteinModelPortal	Q8NFI3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NFI3. 1 interaction.
STRING	9606.ENSP00000300682.
Protein family/group databases
CAZy	GH85. Glycoside Hydrolase Family 85.
PTM databases
PhosphoSite	Q8NFI3.
Polymorphism databases
DMDM	74715557.
Proteomic databases
PaxDb	Q8NFI3.
PeptideAtlas	Q8NFI3.
PRIDE	Q8NFI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000579016; ENSP00000462333; ENSG00000167280.
GeneID	64772.
KEGG	hsa:64772.
UCSC	uc002jwu.1. human. uc002jwv.3. human.
Organism-specific databases
CTD	64772.
GeneCards	GC17P077071.
HGNC	HGNC:24622. ENGASE.
HPA	HPA021551.
MIM	611898. gene.
neXtProt	NX_Q8NFI3.
PharmGKB	PA164719123.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4724.
HOGENOM	HOG000082678.
HOVERGEN	HBG107848.
InParanoid	Q8NFI3.
KO	K01227.
OMA	RCFRIHC.
OrthoDB	EOG44J2HF.
PhylomeDB	Q8NFI3.
Gene expression databases
ArrayExpress	Q8NFI3.
Bgee	Q8NFI3.
Genevestigator	Q8NFI3.
Family and domain databases
InterPro	IPR001357. BRCT_dom. IPR005201. Glyco_hydro_85. [Graphical view]
Pfam	PF03644. Glyco_hydro_85. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8NFI3.
ChEMBL	CHEMBL5172.
ChiTaRS	ENGASE. human.
GenomeRNAi	64772.
NextBio	66783.
SOURCE	Search...

Entry information

Entry name

ENASE_HUMAN

Accession

Primary (citable) accession number: Q8NFI3
Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.