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Protein

Cytosolic endo-beta-N-acetylglucosaminidase

Gene

ENGASE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.1 Publication

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.96. 2681.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Short name:
ENGase
Gene namesi
Name:ENGASE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:24622. ENGASE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164719123.

Polymorphism and mutation databases

BioMutaiENGASE.
DMDMi74715557.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 743743Cytosolic endo-beta-N-acetylglucosaminidasePRO_0000328867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei66 – 661Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8NFI3.
PaxDbiQ8NFI3.
PeptideAtlasiQ8NFI3.
PRIDEiQ8NFI3.

PTM databases

PhosphoSiteiQ8NFI3.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in thymus and spleen.1 Publication

Gene expression databases

BgeeiQ8NFI3.
ExpressionAtlasiQ8NFI3. baseline and differential.
GenevisibleiQ8NFI3. HS.

Organism-specific databases

HPAiHPA021551.

Interactioni

Protein-protein interaction databases

IntActiQ8NFI3. 2 interactions.
STRINGi9606.ENSP00000462333.

Structurei

3D structure databases

ProteinModelPortaliQ8NFI3.
SMRiQ8NFI3. Positions 80-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 38393BRCTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 85 family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4724.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8NFI3.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG7KQ218.
PhylomeDBiQ8NFI3.
TreeFamiTF314391.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR005201. Glyco_hydro_85.
[Graphical view]
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI
60 70 80 90 100
KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL
110 120 130 140 150
EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY
160 170 180 190 200
AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR
210 220 230 240 250
LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP
260 270 280 290 300
FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF
310 320 330 340 350
FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL
360 370 380 390 400
IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF
410 420 430 440 450
VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR
460 470 480 490 500
THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK
510 520 530 540 550
LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA
560 570 580 590 600
RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR
610 620 630 640 650
LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH
660 670 680 690 700
WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL
710 720 730 740
LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA
Length:743
Mass (Da):83,987
Last modified:October 1, 2002 - v1
Checksum:i384DA533AE549FC5
GO
Isoform 2 (identifier: Q8NFI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
     378-743: Missing.

Note: No experimental confirmation available.
Show »
Length:377
Mass (Da):43,107
Checksum:iA60D0AE20C2EDD79
GO
Isoform 3 (identifier: Q8NFI3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
     407-743: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):46,090
Checksum:i4EB91EC292C1A61A
GO

Sequence cautioni

The sequence AAH24213.2 differs from that shown. Reason: Frameshift at position 694. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521F → L in BAB15158 (PubMed:14702039).Curated
Sequence conflicti224 – 2241T → A in CAH56405 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti596 – 5961S → N.1 Publication
Corresponds to variant rs4789879 [ dbSNP | Ensembl ].
VAR_060188
Natural varianti731 – 7311E → K.
Corresponds to variant rs11871357 [ dbSNP | Ensembl ].
VAR_060189

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei347 – 40660SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3. 1 PublicationVSP_032835Add
BLAST
Alternative sequencei364 – 37714GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2. 1 PublicationVSP_032836Add
BLAST
Alternative sequencei378 – 743366Missing in isoform 2. 1 PublicationVSP_032837Add
BLAST
Alternative sequencei407 – 743337Missing in isoform 3. 1 PublicationVSP_032838Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512564 mRNA. Translation: AAM80487.1.
AK025518 mRNA. Translation: BAB15158.1.
CH471099 Genomic DNA. Translation: EAW89562.1.
AL110283 mRNA. Translation: CAH56405.1.
BC024213 mRNA. Translation: AAH24213.2. Frameshift.
CCDSiCCDS42394.1. [Q8NFI3-1]
RefSeqiNP_001036038.1. NM_001042573.2. [Q8NFI3-1]
UniGeneiHs.29288.

Genome annotation databases

EnsembliENST00000579016; ENSP00000462333; ENSG00000167280.
GeneIDi64772.
KEGGihsa:64772.
UCSCiuc002jwu.1. human. [Q8NFI3-3]
uc002jwv.4. human. [Q8NFI3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512564 mRNA. Translation: AAM80487.1.
AK025518 mRNA. Translation: BAB15158.1.
CH471099 Genomic DNA. Translation: EAW89562.1.
AL110283 mRNA. Translation: CAH56405.1.
BC024213 mRNA. Translation: AAH24213.2. Frameshift.
CCDSiCCDS42394.1. [Q8NFI3-1]
RefSeqiNP_001036038.1. NM_001042573.2. [Q8NFI3-1]
UniGeneiHs.29288.

3D structure databases

ProteinModelPortaliQ8NFI3.
SMRiQ8NFI3. Positions 80-728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8NFI3. 2 interactions.
STRINGi9606.ENSP00000462333.

Chemistry

BindingDBiQ8NFI3.
ChEMBLiCHEMBL5172.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

PTM databases

PhosphoSiteiQ8NFI3.

Polymorphism and mutation databases

BioMutaiENGASE.
DMDMi74715557.

Proteomic databases

MaxQBiQ8NFI3.
PaxDbiQ8NFI3.
PeptideAtlasiQ8NFI3.
PRIDEiQ8NFI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000579016; ENSP00000462333; ENSG00000167280.
GeneIDi64772.
KEGGihsa:64772.
UCSCiuc002jwu.1. human. [Q8NFI3-3]
uc002jwv.4. human. [Q8NFI3-1]

Organism-specific databases

CTDi64772.
GeneCardsiGC17P077071.
HGNCiHGNC:24622. ENGASE.
HPAiHPA021551.
MIMi611898. gene.
neXtProtiNX_Q8NFI3.
PharmGKBiPA164719123.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG4724.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8NFI3.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG7KQ218.
PhylomeDBiQ8NFI3.
TreeFamiTF314391.

Enzyme and pathway databases

BRENDAi3.2.1.96. 2681.

Miscellaneous databases

ChiTaRSiENGASE. human.
GenomeRNAii64772.
NextBioi66783.
PROiQ8NFI3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8NFI3.
ExpressionAtlasiQ8NFI3. baseline and differential.
GenevisibleiQ8NFI3. HS.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR005201. Glyco_hydro_85.
[Graphical view]
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol."
    Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S., Inoue Y., Emori Y.
    Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Hepatoma.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-680 (ISOFORM 3).
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 486-743 (ISOFORM 1), VARIANT ASN-596.
    Tissue: Skin.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiENASE_HUMAN
AccessioniPrimary (citable) accession number: Q8NFI3
Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.