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Q8NFI3

- ENASE_HUMAN

UniProt

Q8NFI3 - ENASE_HUMAN

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Protein
Cytosolic endo-beta-N-acetylglucosaminidase
Gene
ENGASE
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.1 Publication

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

  1. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH85. Glycoside Hydrolase Family 85.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
    Short name:
    ENGase
    Gene namesi
    Name:ENGASE
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:24622. ENGASE.

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA164719123.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 743743Cytosolic endo-beta-N-acetylglucosaminidase
    PRO_0000328867Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei66 – 661Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8NFI3.
    PaxDbiQ8NFI3.
    PeptideAtlasiQ8NFI3.
    PRIDEiQ8NFI3.

    PTM databases

    PhosphoSiteiQ8NFI3.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed at higher level in thymus and spleen.1 Publication

    Gene expression databases

    ArrayExpressiQ8NFI3.
    BgeeiQ8NFI3.
    GenevestigatoriQ8NFI3.

    Organism-specific databases

    HPAiHPA021551.

    Interactioni

    Protein-protein interaction databases

    BioGridi122283. 1 interaction.
    IntActiQ8NFI3. 2 interactions.
    STRINGi9606.ENSP00000300682.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NFI3.
    SMRiQ8NFI3. Positions 91-517.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini291 – 38393BRCT
    Add
    BLAST

    Sequence similaritiesi

    Contains 1 BRCT domain.

    Phylogenomic databases

    eggNOGiCOG4724.
    HOGENOMiHOG000082678.
    HOVERGENiHBG107848.
    InParanoidiQ8NFI3.
    KOiK01227.
    OMAiPTKLARW.
    OrthoDBiEOG7KQ218.
    PhylomeDBiQ8NFI3.
    TreeFamiTF314391.

    Family and domain databases

    InterProiIPR001357. BRCT_dom.
    IPR005201. Glyco_hydro_85.
    [Graphical view]
    PfamiPF03644. Glyco_hydro_85. 1 hit.
    [Graphical view]
    PROSITEiPS50172. BRCT. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NFI3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI    50
    KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL 100
    EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY 150
    AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR 200
    LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP 250
    FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 300
    FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL 350
    IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF 400
    VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR 450
    THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK 500
    LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA 550
    RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR 600
    LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH 650
    WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL 700
    LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA 743
    Length:743
    Mass (Da):83,987
    Last modified:October 1, 2002 - v1
    Checksum:i384DA533AE549FC5
    GO
    Isoform 2 (identifier: Q8NFI3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
         378-743: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:377
    Mass (Da):43,107
    Checksum:iA60D0AE20C2EDD79
    GO
    Isoform 3 (identifier: Q8NFI3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
         407-743: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:406
    Mass (Da):46,090
    Checksum:i4EB91EC292C1A61A
    GO

    Sequence cautioni

    The sequence AAH24213.2 differs from that shown. Reason: Frameshift at position 694.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti596 – 5961S → N.1 Publication
    Corresponds to variant rs4789879 [ dbSNP | Ensembl ].
    VAR_060188
    Natural varianti731 – 7311E → K.
    Corresponds to variant rs11871357 [ dbSNP | Ensembl ].
    VAR_060189

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei347 – 40660SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3.
    VSP_032835Add
    BLAST
    Alternative sequencei364 – 37714GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2.
    VSP_032836Add
    BLAST
    Alternative sequencei378 – 743366Missing in isoform 2.
    VSP_032837Add
    BLAST
    Alternative sequencei407 – 743337Missing in isoform 3.
    VSP_032838Add
    BLAST

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521F → L in BAB15158. 1 Publication
    Sequence conflicti224 – 2241T → A in CAH56405. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF512564 mRNA. Translation: AAM80487.1.
    AK025518 mRNA. Translation: BAB15158.1.
    CH471099 Genomic DNA. Translation: EAW89562.1.
    AL110283 mRNA. Translation: CAH56405.1.
    BC024213 mRNA. Translation: AAH24213.2. Frameshift.
    CCDSiCCDS42394.1. [Q8NFI3-1]
    RefSeqiNP_001036038.1. NM_001042573.2. [Q8NFI3-1]
    UniGeneiHs.29288.

    Genome annotation databases

    EnsembliENST00000579016; ENSP00000462333; ENSG00000167280. [Q8NFI3-1]
    GeneIDi64772.
    KEGGihsa:64772.
    UCSCiuc002jwu.1. human. [Q8NFI3-3]
    uc002jwv.4. human. [Q8NFI3-1]

    Polymorphism databases

    DMDMi74715557.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF512564 mRNA. Translation: AAM80487.1 .
    AK025518 mRNA. Translation: BAB15158.1 .
    CH471099 Genomic DNA. Translation: EAW89562.1 .
    AL110283 mRNA. Translation: CAH56405.1 .
    BC024213 mRNA. Translation: AAH24213.2 . Frameshift.
    CCDSi CCDS42394.1. [Q8NFI3-1 ]
    RefSeqi NP_001036038.1. NM_001042573.2. [Q8NFI3-1 ]
    UniGenei Hs.29288.

    3D structure databases

    ProteinModelPortali Q8NFI3.
    SMRi Q8NFI3. Positions 91-517.
    ModBasei Search...

    Protein-protein interaction databases

    BioGridi 122283. 1 interaction.
    IntActi Q8NFI3. 2 interactions.
    STRINGi 9606.ENSP00000300682.

    Chemistry

    BindingDBi Q8NFI3.
    ChEMBLi CHEMBL5172.

    Protein family/group databases

    CAZyi GH85. Glycoside Hydrolase Family 85.

    PTM databases

    PhosphoSitei Q8NFI3.

    Polymorphism databases

    DMDMi 74715557.

    Proteomic databases

    MaxQBi Q8NFI3.
    PaxDbi Q8NFI3.
    PeptideAtlasi Q8NFI3.
    PRIDEi Q8NFI3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000579016 ; ENSP00000462333 ; ENSG00000167280 . [Q8NFI3-1 ]
    GeneIDi 64772.
    KEGGi hsa:64772.
    UCSCi uc002jwu.1. human. [Q8NFI3-3 ]
    uc002jwv.4. human. [Q8NFI3-1 ]

    Organism-specific databases

    CTDi 64772.
    GeneCardsi GC17P077071.
    HGNCi HGNC:24622. ENGASE.
    HPAi HPA021551.
    MIMi 611898. gene.
    neXtProti NX_Q8NFI3.
    PharmGKBi PA164719123.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4724.
    HOGENOMi HOG000082678.
    HOVERGENi HBG107848.
    InParanoidi Q8NFI3.
    KOi K01227.
    OMAi PTKLARW.
    OrthoDBi EOG7KQ218.
    PhylomeDBi Q8NFI3.
    TreeFami TF314391.

    Miscellaneous databases

    ChiTaRSi ENGASE. human.
    GenomeRNAii 64772.
    NextBioi 66783.
    PROi Q8NFI3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8NFI3.
    Bgeei Q8NFI3.
    Genevestigatori Q8NFI3.

    Family and domain databases

    InterProi IPR001357. BRCT_dom.
    IPR005201. Glyco_hydro_85.
    [Graphical view ]
    Pfami PF03644. Glyco_hydro_85. 1 hit.
    [Graphical view ]
    PROSITEi PS50172. BRCT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol."
      Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S., Inoue Y., Emori Y.
      Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Hepatoma.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-680 (ISOFORM 3).
      Tissue: Testis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 486-743 (ISOFORM 1), VARIANT ASN-596.
      Tissue: Skin.
    6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiENASE_HUMAN
    AccessioniPrimary (citable) accession number: Q8NFI3
    Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 1, 2002
    Last modified: July 9, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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