Reviewed, UniProtKB/Swiss-Prot Q8NFI3 (ENASE_HUMAN)

Names and origin

Protein names

Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase
      Short name=ENGase
    EC=3.2.1.96

Gene names

Name:

ENGASE

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol. Ref.1
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Cytoplasm › cytosol By similarity.
Tissue specificity	Widely expressed. Expressed at higher level in thymus and spleen. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 85 family. Contains 1 BRCT domain.
Sequence caution	The sequence AAH24213.2 differs from that shown. Reason: Frameshift at position 694.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NFI3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NFI3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
     378-743: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NFI3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
     407-743: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Cytosolic endo-beta-N-acetylglucosaminidase		PRO_0000328867
Regions
	Domain	291 – 383	93	BRCT
Natural variations
	Alternative sequence	347 – 406	60	SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3.		VSP_032835
	Alternative sequence	364 – 377	14	GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2.		VSP_032836
	Alternative sequence	378 – 743	366	Missing in isoform 2.		VSP_032837
	Alternative sequence	407 – 743	337	Missing in isoform 3.		VSP_032838
Experimental info
	Sequence conflict	152	1	F → L in BAB15158. Ref.2
	Sequence conflict	224	1	T → A in CAH56405. Ref.4
	Sequence conflict	596	1	S → N in AAH24213. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 384DA533AE549FC5	FASTA	743	83,987
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL IRKHGFSVAL 370 380 390 400 410 420 FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF VTSFCLGMGA RRVCYGQEEA 430 440 450 460 470 480 VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR THCCLEDAWH GGSSLLVRGV IPPEVGNVAV 490 500 510 520 530 540 RLFSLQAPVP PKIYLSMVYK LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR 550 560 570 580 590 600 PLRVPPTKLA RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR 610 620 630 640 650 660 LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH WSFLLSQVRC 670 680 690 700 710 720 FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL LVEAAGPGQD RRMEFLVEPV 730 740 PKEGFRVPQA EWGRAVLLYS APA
	Isoform 2. Checksum: A60D0AE20C2EDD79	FASTA	377	43,107
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL IRKHGFSVAL 370 FAPSCSVFPG VGNLLCC
	Isoform 3. Checksum: 4EB91EC292C1A61A	FASTA	406	46,090
	10 20 30 40 50 60 MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR 70 80 90 100 110 120 EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS 130 140 150 160 170 180 QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA 190 200 210 220 230 240 HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL 250 260 270 280 290 300 SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF 310 320 330 340 350 360 FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKVGGG FRPRASGPVP 370 380 390 400 PLGPHFLMDL PFPSAPQRND SSCSSQSGDP VALRNRCPAP AKLCPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol." Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S., Inoue Y., Emori Y. Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002) [PubMed: 12114544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hepatoma.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-680 (ISOFORM 3). Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 486-743 (ISOFORM 1). Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF512564 mRNA. Translation: AAM80487.1. AK025518 mRNA. Translation: BAB15158.1. CH471099 Genomic DNA. Translation: EAW89562.1. AL110283 mRNA. Translation: CAH56405.1. BC024213 mRNA. Translation: AAH24213.2. Frameshift.
IPI	IPI00168838. IPI00874016. IPI00889689.
RefSeq	NP_001036038.1.
UniGene	Hs.29288
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH85. Glycoside Hydrolase Family 85.
Proteomic databases
PeptideAtlas	Q8NFI3.
PRIDE	Q8NFI3.
Genome annotation databases
Ensembl	ENSG00000167280. Homo sapiens. [Contig view]
GeneID	64772.
KEGG	hsa:64772.
Organism-specific databases
GeneCards	GC17P074582.
HGNC	HGNC:24622. ENGASE.
MIM	611898. gene.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8NFI3.
HOVERGEN	Q8NFI3.
OMA	Q8NFI3. SAQEIQP.
Enzyme and pathway databases
BRENDA	3.2.1.96. 247.
Gene expression databases
ArrayExpress	Q8NFI3.
Bgee	Q8NFI3.
Family and domain databases
InterPro	IPR001357. BRCT. IPR005201. Glyco_hydro_85. [Graphical view]
PANTHER	PTHR13246. Glyco_hydro_85. 1 hit.
Pfam	PF03644. Glyco_hydro_85. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	66783.
SOURCE	Search...

Entry information

Entry name

ENASE_HUMAN

Accession

Primary (citable) accession number: Q8NFI3
Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)