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Protein

Cytosolic endo-beta-N-acetylglucosaminidase

Gene

ENGASE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.1 Publication

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciZFISH:HS09534-MONOMER.
BRENDAi3.2.1.96. 2681.
ReactomeiR-HSA-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Short name:
ENGase
Gene namesi
Name:ENGASE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:24622. ENGASE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi64772.
OpenTargetsiENSG00000167280.
PharmGKBiPA164719123.

Chemistry databases

ChEMBLiCHEMBL5172.

Polymorphism and mutation databases

BioMutaiENGASE.
DMDMi74715557.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003288671 – 743Cytosolic endo-beta-N-acetylglucosaminidaseAdd BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei66PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8NFI3.
MaxQBiQ8NFI3.
PaxDbiQ8NFI3.
PeptideAtlasiQ8NFI3.
PRIDEiQ8NFI3.

PTM databases

iPTMnetiQ8NFI3.
PhosphoSitePlusiQ8NFI3.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in thymus and spleen.1 Publication

Gene expression databases

BgeeiENSG00000167280.
ExpressionAtlasiQ8NFI3. baseline and differential.

Organism-specific databases

HPAiHPA021551.

Interactioni

Protein-protein interaction databases

BioGridi122283. 1 interactor.
IntActiQ8NFI3. 2 interactors.
STRINGi9606.ENSP00000462333.

Chemistry databases

BindingDBiQ8NFI3.

Structurei

3D structure databases

ProteinModelPortaliQ8NFI3.
SMRiQ8NFI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini291 – 383BRCTPROSITE-ProRule annotationAdd BLAST93

Sequence similaritiesi

Belongs to the glycosyl hydrolase 85 family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2331. Eukaryota.
COG4724. LUCA.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8NFI3.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG091G0A7Z.
PhylomeDBiQ8NFI3.
TreeFamiTF314391.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR032979. ENGase.
IPR005201. Glyco_hydro_85.
[Graphical view]
PANTHERiPTHR13246. PTHR13246. 1 hit.
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI
60 70 80 90 100
KDEEEETVFR EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL
110 120 130 140 150
EDGFNVALEP LACRQPPLSS QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY
160 170 180 190 200
AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA HRHGVCVLGT FITEWNEGGR
210 220 230 240 250
LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL SLAAVGNMPP
260 270 280 290 300
FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF
310 320 330 340 350
FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL
360 370 380 390 400
IRKHGFSVAL FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF
410 420 430 440 450
VTSFCLGMGA RRVCYGQEEA VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR
460 470 480 490 500
THCCLEDAWH GGSSLLVRGV IPPEVGNVAV RLFSLQAPVP PKIYLSMVYK
510 520 530 540 550
LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR PLRVPPTKLA
560 570 580 590 600
RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR
610 620 630 640 650
LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH
660 670 680 690 700
WSFLLSQVRC FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL
710 720 730 740
LVEAAGPGQD RRMEFLVEPV PKEGFRVPQA EWGRAVLLYS APA
Length:743
Mass (Da):83,987
Last modified:October 1, 2002 - v1
Checksum:i384DA533AE549FC5
GO
Isoform 2 (identifier: Q8NFI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-377: GWVYECLEKKDFFQ → SCSVFPGVGNLLCC
     378-743: Missing.

Note: No experimental confirmation available.
Show »
Length:377
Mass (Da):43,107
Checksum:iA60D0AE20C2EDD79
GO
Isoform 3 (identifier: Q8NFI3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-406: SLELIRKHGF...SLPFVTSFCL → VGGGFRPRAS...CPAPAKLCPH
     407-743: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):46,090
Checksum:i4EB91EC292C1A61A
GO

Sequence cautioni

The sequence AAH24213 differs from that shown. Reason: Frameshift at position 694.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152F → L in BAB15158 (PubMed:14702039).Curated1
Sequence conflicti224T → A in CAH56405 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060188596S → N.1 PublicationCorresponds to variant rs4789879dbSNPEnsembl.1
Natural variantiVAR_060189731E → K.Corresponds to variant rs11871357dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032835347 – 406SLELI…TSFCL → VGGGFRPRASGPVPPLGPHF LMDLPFPSAPQRNDSSCSSQ SGDPVALRNRCPAPAKLCPH in isoform 3. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_032836364 – 377GWVYE…KDFFQ → SCSVFPGVGNLLCC in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_032837378 – 743Missing in isoform 2. 1 PublicationAdd BLAST366
Alternative sequenceiVSP_032838407 – 743Missing in isoform 3. 1 PublicationAdd BLAST337

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512564 mRNA. Translation: AAM80487.1.
AK025518 mRNA. Translation: BAB15158.1.
CH471099 Genomic DNA. Translation: EAW89562.1.
AL110283 mRNA. Translation: CAH56405.1.
BC024213 mRNA. Translation: AAH24213.2. Frameshift.
CCDSiCCDS42394.1. [Q8NFI3-1]
RefSeqiNP_001036038.1. NM_001042573.2. [Q8NFI3-1]
UniGeneiHs.29288.

Genome annotation databases

EnsembliENST00000579016; ENSP00000462333; ENSG00000167280. [Q8NFI3-1]
GeneIDi64772.
KEGGihsa:64772.
UCSCiuc002jwv.5. human. [Q8NFI3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF512564 mRNA. Translation: AAM80487.1.
AK025518 mRNA. Translation: BAB15158.1.
CH471099 Genomic DNA. Translation: EAW89562.1.
AL110283 mRNA. Translation: CAH56405.1.
BC024213 mRNA. Translation: AAH24213.2. Frameshift.
CCDSiCCDS42394.1. [Q8NFI3-1]
RefSeqiNP_001036038.1. NM_001042573.2. [Q8NFI3-1]
UniGeneiHs.29288.

3D structure databases

ProteinModelPortaliQ8NFI3.
SMRiQ8NFI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122283. 1 interactor.
IntActiQ8NFI3. 2 interactors.
STRINGi9606.ENSP00000462333.

Chemistry databases

BindingDBiQ8NFI3.
ChEMBLiCHEMBL5172.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

PTM databases

iPTMnetiQ8NFI3.
PhosphoSitePlusiQ8NFI3.

Polymorphism and mutation databases

BioMutaiENGASE.
DMDMi74715557.

Proteomic databases

EPDiQ8NFI3.
MaxQBiQ8NFI3.
PaxDbiQ8NFI3.
PeptideAtlasiQ8NFI3.
PRIDEiQ8NFI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000579016; ENSP00000462333; ENSG00000167280. [Q8NFI3-1]
GeneIDi64772.
KEGGihsa:64772.
UCSCiuc002jwv.5. human. [Q8NFI3-1]

Organism-specific databases

CTDi64772.
DisGeNETi64772.
GeneCardsiENGASE.
HGNCiHGNC:24622. ENGASE.
HPAiHPA021551.
MIMi611898. gene.
neXtProtiNX_Q8NFI3.
OpenTargetsiENSG00000167280.
PharmGKBiPA164719123.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2331. Eukaryota.
COG4724. LUCA.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8NFI3.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG091G0A7Z.
PhylomeDBiQ8NFI3.
TreeFamiTF314391.

Enzyme and pathway databases

BioCyciZFISH:HS09534-MONOMER.
BRENDAi3.2.1.96. 2681.
ReactomeiR-HSA-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Miscellaneous databases

ChiTaRSiENGASE. human.
GenomeRNAii64772.
PROiQ8NFI3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167280.
ExpressionAtlasiQ8NFI3. baseline and differential.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR032979. ENGase.
IPR005201. Glyco_hydro_85.
[Graphical view]
PANTHERiPTHR13246. PTHR13246. 1 hit.
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENASE_HUMAN
AccessioniPrimary (citable) accession number: Q8NFI3
Secondary accession number(s): Q659F0, Q8TB86, Q9H6U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.