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Protein

Nucleoporin NUP53

Gene

NUP35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159227. Transport of the SLBP independent Mature mRNA.
R-HSA-159230. Transport of the SLBP Dependant Mature mRNA.
R-HSA-159231. Transport of Mature mRNA Derived from an Intronless Transcript.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168271. Transport of Ribonucleoproteins into the Host Nucleus.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-191859. snRNP Assembly.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP53
Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name:
MP-44
Nuclear pore complex protein Nup53
Nucleoporin Nup35
Gene namesi
Name:NUP35
Synonyms:MP44, NUP53
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:29797. NUP35.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163002.
PharmGKBiPA134861481.

Polymorphism and mutation databases

DMDMi74730292.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002342941 – 326Nucleoporin NUP53Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei106PhosphothreonineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei129PhosphothreonineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei252PhosphoserineBy similarity1
Modified residuei259PhosphoserineBy similarity1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei275PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1
Modified residuei280PhosphothreonineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NFH5.
MaxQBiQ8NFH5.
PaxDbiQ8NFH5.
PeptideAtlasiQ8NFH5.
PRIDEiQ8NFH5.

PTM databases

iPTMnetiQ8NFH5.
PhosphoSitePlusiQ8NFH5.

Expressioni

Gene expression databases

BgeeiENSG00000163002.
CleanExiHS_NUP35.
ExpressionAtlasiQ8NFH5. baseline and differential.
GenevisibleiQ8NFH5. HS.

Organism-specific databases

HPAiHPA018401.
HPA018410.
HPA018441.

Interactioni

Subunit structurei

Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KCTD9Q7L2735EBI-9050429,EBI-4397613

Protein-protein interaction databases

BioGridi126190. 71 interactors.
DIPiDIP-47308N.
IntActiQ8NFH5. 21 interactors.
MINTiMINT-4301788.
STRINGi9606.ENSP00000295119.

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi172 – 174Combined sources3
Beta strandi175 – 179Combined sources5
Helixi183 – 185Combined sources3
Helixi186 – 193Combined sources8
Beta strandi199 – 204Combined sources6
Beta strandi206 – 217Combined sources12
Helixi218 – 225Combined sources8
Turni226 – 229Combined sources4
Beta strandi230 – 232Combined sources3
Turni233 – 235Combined sources3
Beta strandi236 – 242Combined sources7
Helixi246 – 249Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LIRX-ray2.46A/B151-266[»]
ProteinModelPortaliQ8NFH5.
SMRiQ8NFH5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 250RRM Nup35-typePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the Nup53 family.Curated
Contains 1 RRM Nup35-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4285. Eukaryota.
ENOG4111ARE. LUCA.
GeneTreeiENSGT00390000005923.
HOGENOMiHOG000231921.
HOVERGENiHBG060396.
InParanoidiQ8NFH5.
KOiK14313.
OMAiSSYHTND.
OrthoDBiEOG091G0DAQ.
PhylomeDBiQ8NFH5.
TreeFamiTF325369.

Family and domain databases

InterProiIPR017389. Nucleoporin_NUP53.
IPR012677. Nucleotide-bd_a/b_plait.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PfamiPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS51472. RRM_NUP35. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ
60 70 80 90 100
PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS
110 120 130 140 150
PGLGSTPLTS RRQPNISVMQ SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL
160 170 180 190 200
SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL
210 220 230 240 250
KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME
260 270 280 290 300
SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY
310 320
QVISDRQTPK KDESLVSKAM EYMFGW
Length:326
Mass (Da):34,774
Last modified:October 1, 2002 - v1
Checksum:i6420B0EF1AE31013
GO
Isoform 2 (identifier: Q8NFH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: No experimental confirmation available.
Show »
Length:309
Mass (Da):33,120
Checksum:iBA43C9E8B0BD3BD1
GO
Isoform 3 (identifier: Q8NFH5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: Missing.

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):21,142
Checksum:i96265A68DC195F33
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313E → G in AAL86379 (Ref. 2) Curated1
Sequence conflicti318K → Q in AAL86379 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0562101 – 135Missing in isoform 3. 1 PublicationAdd BLAST135
Alternative sequenceiVSP_0562111 – 17Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514993 mRNA. Translation: AAM76704.1.
AF411516 mRNA. Translation: AAL86379.1.
AK298199 mRNA. Translation: BAG60469.1.
AK302350 mRNA. Translation: BAG63676.1.
AC064871 Genomic DNA. Translation: AAY24198.1.
AC079249 Genomic DNA. Translation: AAX88896.1.
BC047029 mRNA. Translation: AAH47029.1.
BC061698 mRNA. Translation: AAH61698.1.
CCDSiCCDS2290.1. [Q8NFH5-1]
CCDS74614.1. [Q8NFH5-2]
RefSeqiNP_001274513.1. NM_001287584.1. [Q8NFH5-2]
NP_001274514.1. NM_001287585.1.
NP_612142.2. NM_138285.4. [Q8NFH5-1]
XP_006712317.1. XM_006712254.2. [Q8NFH5-2]
XP_011508878.1. XM_011510576.2. [Q8NFH5-2]
XP_011508879.1. XM_011510577.2. [Q8NFH5-2]
UniGeneiHs.180591.

Genome annotation databases

EnsembliENST00000295119; ENSP00000295119; ENSG00000163002. [Q8NFH5-1]
ENST00000409798; ENSP00000387305; ENSG00000163002. [Q8NFH5-2]
GeneIDi129401.
KEGGihsa:129401.
UCSCiuc002upf.5. human. [Q8NFH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514993 mRNA. Translation: AAM76704.1.
AF411516 mRNA. Translation: AAL86379.1.
AK298199 mRNA. Translation: BAG60469.1.
AK302350 mRNA. Translation: BAG63676.1.
AC064871 Genomic DNA. Translation: AAY24198.1.
AC079249 Genomic DNA. Translation: AAX88896.1.
BC047029 mRNA. Translation: AAH47029.1.
BC061698 mRNA. Translation: AAH61698.1.
CCDSiCCDS2290.1. [Q8NFH5-1]
CCDS74614.1. [Q8NFH5-2]
RefSeqiNP_001274513.1. NM_001287584.1. [Q8NFH5-2]
NP_001274514.1. NM_001287585.1.
NP_612142.2. NM_138285.4. [Q8NFH5-1]
XP_006712317.1. XM_006712254.2. [Q8NFH5-2]
XP_011508878.1. XM_011510576.2. [Q8NFH5-2]
XP_011508879.1. XM_011510577.2. [Q8NFH5-2]
UniGeneiHs.180591.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LIRX-ray2.46A/B151-266[»]
ProteinModelPortaliQ8NFH5.
SMRiQ8NFH5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126190. 71 interactors.
DIPiDIP-47308N.
IntActiQ8NFH5. 21 interactors.
MINTiMINT-4301788.
STRINGi9606.ENSP00000295119.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ8NFH5.
PhosphoSitePlusiQ8NFH5.

Polymorphism and mutation databases

DMDMi74730292.

Proteomic databases

EPDiQ8NFH5.
MaxQBiQ8NFH5.
PaxDbiQ8NFH5.
PeptideAtlasiQ8NFH5.
PRIDEiQ8NFH5.

Protocols and materials databases

DNASUi129401.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295119; ENSP00000295119; ENSG00000163002. [Q8NFH5-1]
ENST00000409798; ENSP00000387305; ENSG00000163002. [Q8NFH5-2]
GeneIDi129401.
KEGGihsa:129401.
UCSCiuc002upf.5. human. [Q8NFH5-1]

Organism-specific databases

CTDi129401.
GeneCardsiNUP35.
HGNCiHGNC:29797. NUP35.
HPAiHPA018401.
HPA018410.
HPA018441.
MIMi608140. gene.
neXtProtiNX_Q8NFH5.
OpenTargetsiENSG00000163002.
PharmGKBiPA134861481.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4285. Eukaryota.
ENOG4111ARE. LUCA.
GeneTreeiENSGT00390000005923.
HOGENOMiHOG000231921.
HOVERGENiHBG060396.
InParanoidiQ8NFH5.
KOiK14313.
OMAiSSYHTND.
OrthoDBiEOG091G0DAQ.
PhylomeDBiQ8NFH5.
TreeFamiTF325369.

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159227. Transport of the SLBP independent Mature mRNA.
R-HSA-159230. Transport of the SLBP Dependant Mature mRNA.
R-HSA-159231. Transport of Mature mRNA Derived from an Intronless Transcript.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168271. Transport of Ribonucleoproteins into the Host Nucleus.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-191859. snRNP Assembly.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.

Miscellaneous databases

GeneWikiiNUP35.
GenomeRNAii129401.
PROiQ8NFH5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163002.
CleanExiHS_NUP35.
ExpressionAtlasiQ8NFH5. baseline and differential.
GenevisibleiQ8NFH5. HS.

Family and domain databases

InterProiIPR017389. Nucleoporin_NUP53.
IPR012677. Nucleotide-bd_a/b_plait.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PfamiPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS51472. RRM_NUP35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP53_HUMAN
AccessioniPrimary (citable) accession number: Q8NFH5
Secondary accession number(s): B4DP57
, B4DYB4, Q4ZFZ9, Q53S95, Q8TDJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.