Q8NFH5 (NUP53_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoporin NUP53

Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name=MP-44
Nuclear pore complex protein Nup53
Nucleoporin Nup35

Gene names

Name:	NUP35
Synonyms:	MP44, NUP53

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. Ref.5
Subunit structure	Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct. Ref.5 Ref.7
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein. Note: Tightly associated with the nuclear membrane and lamina. Ref.1 Ref.5
Sequence similarities	Belongs to the Nup53 family. Contains 1 RRM Nup35-type domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome cytokine-mediated signaling pathway Traceable author statement. Source: Reactome glucose transport Traceable author statement. Source: Reactome mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of glucose transport Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome viral reproductive process Traceable author statement. Source: Reactome
Cellular_component	intermediate filament cytoskeleton Inferred from direct assay. Source: HPA nuclear membrane Inferred from direct assay. Source: HPA nuclear pore Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: HPA
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Nucleoporin NUP53		PRO_0000234294
Regions
	Domain	170 – 250	81	RRM Nup35-type
Amino acid modifications
	Modified residue	66	1	Phosphoserine Ref.11
	Modified residue	73	1	Phosphoserine Ref.6 Ref.11 Ref.13
	Modified residue	99	1	Phosphoserine Ref.9
	Modified residue	100	1	Phosphoserine Ref.9 Ref.10 Ref.11
	Modified residue	106	1	Phosphothreonine Ref.9 Ref.11
	Modified residue	121	1	Phosphoserine Ref.11
	Modified residue	129	1	Phosphothreonine Ref.9
	Modified residue	138	1	Phosphoserine Ref.9
	Modified residue	265	1	Phosphothreonine Ref.9
	Modified residue	273	1	Phosphothreonine Ref.9 Ref.11
	Modified residue	279	1	Phosphoserine Ref.9 Ref.11
	Modified residue	280	1	Phosphothreonine Ref.9 Ref.11
	Modified residue	308	1	Phosphothreonine Ref.9
Experimental info
	Sequence conflict	313	1	E → G in AAL86379. Ref.2
	Sequence conflict	318	1	K → Q in AAL86379. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8NFH5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6420B0EF1AE31013	FASTA	326	34,774
	10 20 30 40 50 60 MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ PRSISGPSVG 70 80 90 100 110 120 VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS PGLGSTPLTS RRQPNISVMQ 130 140 150 160 170 180 SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG 190 200 210 220 230 240 FPQASASYIL LQFAQYGNIL KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV 250 260 270 280 290 300 KPCIDKSVME SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY 310 320 QVISDRQTPK KDESLVSKAM EYMFGW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteomic analysis of the mammalian nuclear pore complex." Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. J. Cell Biol. 158:915-927(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"Molecular cloning and expression analysis of human mitotic phosphoprotein 44 gene." Guo J.H., Yu L. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Skin.
[5]	"Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex." Hawryluk-Gara L.A., Shibuya E.K., Wozniak R.W. Mol. Biol. Cell 16:2382-2394(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NUP93, IDENTIFICATION IN A COMPLEX WITH LAMIN B; NUP155 AND NUP205.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells." Mansfeld J., Guettinger S., Hawryluk-Gara L.A., Pante N., Mall M., Galy V., Haselmann U., Muehlhaeusser P., Wozniak R.W., Mattaj I.W., Kutay U., Antonin W. Mol. Cell 22:93-103(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TMEM48.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-100; THR-106; THR-129; SER-138; THR-265; THR-273; SER-279; THR-280 AND THR-308, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-73; SER-100; THR-106; SER-121; THR-273; SER-279 AND THR-280, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF514993 mRNA. Translation: AAM76704.1. AF411516 mRNA. Translation: AAL86379.1. AC064871 Genomic DNA. Translation: AAY24198.1. AC079249 Genomic DNA. Translation: AAX88896.1. BC047029 mRNA. Translation: AAH47029.1. BC061698 mRNA. Translation: AAH61698.1.
IPI	IPI00329650.
RefSeq	NP_612142.2. NM_138285.3.
UniGene	Hs.180591.
3D structure databases
ProteinModelPortal	Q8NFH5.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47308N.
MINT	MINT-4301788.
STRING	9606.ENSP00000295119.
PTM databases
PhosphoSite	Q8NFH5.
Polymorphism databases
DMDM	74730292.
Proteomic databases
PaxDb	Q8NFH5.
PeptideAtlas	Q8NFH5.
PRIDE	Q8NFH5.
Protocols and materials databases
DNASU	129401.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295119; ENSP00000295119; ENSG00000163002.
GeneID	129401.
KEGG	hsa:129401.
UCSC	uc002upf.3. human.
Organism-specific databases
CTD	129401.
GeneCards	GC02P183982.
HGNC	HGNC:29797. NUP35.
HPA	HPA018401. HPA018410. HPA018441.
MIM	608140. gene.
neXtProt	NX_Q8NFH5.
PharmGKB	PA134861481.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG253574.
HOGENOM	HOG000231921.
HOVERGEN	HBG060396.
InParanoid	Q8NFH5.
KO	K14313.
OMA	ANISVMQ.
OrthoDB	EOG43N7D2.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease. REACT_15518. Transmembrane transport of small molecules. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q8NFH5.
Bgee	Q8NFH5.
CleanEx	HS_NUP35.
Genevestigator	Q8NFH5.
GermOnline	ENSG00000163002. Homo sapiens.
Family and domain databases
InterPro	IPR017389. Nucleoporin_NUP53. IPR007846. RRM_NUP35_dom. [Graphical view]
Pfam	PF05172. Nup35_RRM. 1 hit. [Graphical view]
PIRSF	PIRSF038119. Nucleoporin_NUP53. 1 hit.
PROSITE	PS51472. RRM_NUP35. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	129401.
NextBio	82574.
SOURCE	Search...

Entry information

Entry name

NUP53_HUMAN

Accession

Primary (citable) accession number: Q8NFH5
Secondary accession number(s): Q4ZFZ9, Q53S95, Q8TDJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.