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Q8NFH5

- NUP53_HUMAN

UniProt

Q8NFH5 - NUP53_HUMAN

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Protein

Nucleoporin NUP53

Gene
NUP35, MP44, NUP53
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.1 Publication

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. cytokine-mediated signaling pathway Source: Reactome
  3. glucose transport Source: Reactome
  4. hexose transport Source: Reactome
  5. mitotic cell cycle Source: Reactome
  6. mitotic nuclear envelope disassembly Source: Reactome
  7. mRNA transport Source: UniProtKB-KW
  8. protein transport Source: UniProtKB-KW
  9. regulation of glucose transport Source: Reactome
  10. small molecule metabolic process Source: Reactome
  11. transmembrane transport Source: Reactome
  12. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiREACT_115831. ISG15 antiviral mechanism.
REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
REACT_6190. Rev-mediated nuclear export of HIV RNA.
REACT_6804. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_7991. Vpr-mediated nuclear import of PICs.
REACT_9395. Nuclear import of Rev protein.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP53
Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name:
MP-44
Nuclear pore complex protein Nup53
Nucleoporin Nup35
Gene namesi
Name:NUP35
Synonyms:MP44, NUP53
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:29797. NUP35.

Subcellular locationi

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein
Note: Tightly associated with the nuclear membrane and lamina.2 Publications

GO - Cellular componenti

  1. intermediate filament cytoskeleton Source: HPA
  2. intracellular membrane-bounded organelle Source: HPA
  3. nuclear envelope Source: Reactome
  4. nuclear membrane Source: HPA
  5. nuclear pore Source: UniProtKB-SubCell
  6. nucleus Source: HPA
  7. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134861481.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Nucleoporin NUP53PRO_0000234294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661Phosphoserine1 Publication
Modified residuei73 – 731Phosphoserine3 Publications
Modified residuei99 – 991Phosphoserine1 Publication
Modified residuei100 – 1001Phosphoserine3 Publications
Modified residuei106 – 1061Phosphothreonine2 Publications
Modified residuei121 – 1211Phosphoserine1 Publication
Modified residuei129 – 1291Phosphothreonine1 Publication
Modified residuei138 – 1381Phosphoserine1 Publication
Modified residuei259 – 2591Phosphoserine By similarity
Modified residuei265 – 2651Phosphothreonine1 Publication
Modified residuei273 – 2731Phosphothreonine2 Publications
Modified residuei279 – 2791Phosphoserine2 Publications
Modified residuei280 – 2801Phosphothreonine2 Publications
Modified residuei308 – 3081Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8NFH5.
PaxDbiQ8NFH5.
PeptideAtlasiQ8NFH5.
PRIDEiQ8NFH5.

PTM databases

PhosphoSiteiQ8NFH5.

Expressioni

Gene expression databases

ArrayExpressiQ8NFH5.
BgeeiQ8NFH5.
CleanExiHS_NUP35.
GenevestigatoriQ8NFH5.

Organism-specific databases

HPAiHPA018401.
HPA018410.
HPA018441.

Interactioni

Subunit structurei

Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct.2 Publications

Protein-protein interaction databases

BioGridi126190. 4 interactions.
DIPiDIP-47308N.
IntActiQ8NFH5. 1 interaction.
MINTiMINT-4301788.
STRINGi9606.ENSP00000295119.

Structurei

Secondary structure

1
326
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi172 – 1743
Beta strandi175 – 1795
Helixi183 – 1853
Helixi186 – 1938
Beta strandi199 – 2046
Beta strandi206 – 21712
Helixi218 – 2258
Turni226 – 2294
Beta strandi230 – 2323
Turni233 – 2353
Beta strandi236 – 2427
Helixi246 – 2494

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LIRX-ray2.46A/B151-266[»]
ProteinModelPortaliQ8NFH5.
SMRiQ8NFH5. Positions 169-252.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini170 – 25081RRM Nup35-typeAdd
BLAST

Sequence similaritiesi

Belongs to the Nup53 family.

Phylogenomic databases

eggNOGiNOG253574.
HOGENOMiHOG000231921.
HOVERGENiHBG060396.
InParanoidiQ8NFH5.
KOiK14313.
OMAiANISVMQ.
PhylomeDBiQ8NFH5.
TreeFamiTF325369.

Family and domain databases

InterProiIPR017389. Nucleoporin_NUP53.
IPR007846. RRM_NUP35_dom.
[Graphical view]
PfamiPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PIRSFiPIRSF038119. Nucleoporin_NUP53. 1 hit.
PROSITEiPS51472. RRM_NUP35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NFH5-1 [UniParc]FASTAAdd to Basket

« Hide

MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ    50
PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS 100
PGLGSTPLTS RRQPNISVMQ SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL 150
SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL 200
KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME 250
SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY 300
QVISDRQTPK KDESLVSKAM EYMFGW 326
Length:326
Mass (Da):34,774
Last modified:October 1, 2002 - v1
Checksum:i6420B0EF1AE31013
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131E → G in AAL86379. 1 Publication
Sequence conflicti318 – 3181K → Q in AAL86379. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF514993 mRNA. Translation: AAM76704.1.
AF411516 mRNA. Translation: AAL86379.1.
AC064871 Genomic DNA. Translation: AAY24198.1.
AC079249 Genomic DNA. Translation: AAX88896.1.
BC047029 mRNA. Translation: AAH47029.1.
BC061698 mRNA. Translation: AAH61698.1.
CCDSiCCDS2290.1.
RefSeqiNP_001274514.1. NM_001287585.1.
NP_612142.2. NM_138285.4.
UniGeneiHs.180591.

Genome annotation databases

EnsembliENST00000295119; ENSP00000295119; ENSG00000163002.
GeneIDi129401.
KEGGihsa:129401.
UCSCiuc002upf.3. human.

Polymorphism databases

DMDMi74730292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF514993 mRNA. Translation: AAM76704.1 .
AF411516 mRNA. Translation: AAL86379.1 .
AC064871 Genomic DNA. Translation: AAY24198.1 .
AC079249 Genomic DNA. Translation: AAX88896.1 .
BC047029 mRNA. Translation: AAH47029.1 .
BC061698 mRNA. Translation: AAH61698.1 .
CCDSi CCDS2290.1.
RefSeqi NP_001274514.1. NM_001287585.1.
NP_612142.2. NM_138285.4.
UniGenei Hs.180591.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LIR X-ray 2.46 A/B 151-266 [» ]
ProteinModelPortali Q8NFH5.
SMRi Q8NFH5. Positions 169-252.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126190. 4 interactions.
DIPi DIP-47308N.
IntActi Q8NFH5. 1 interaction.
MINTi MINT-4301788.
STRINGi 9606.ENSP00000295119.

PTM databases

PhosphoSitei Q8NFH5.

Polymorphism databases

DMDMi 74730292.

Proteomic databases

MaxQBi Q8NFH5.
PaxDbi Q8NFH5.
PeptideAtlasi Q8NFH5.
PRIDEi Q8NFH5.

Protocols and materials databases

DNASUi 129401.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000295119 ; ENSP00000295119 ; ENSG00000163002 .
GeneIDi 129401.
KEGGi hsa:129401.
UCSCi uc002upf.3. human.

Organism-specific databases

CTDi 129401.
GeneCardsi GC02P183982.
HGNCi HGNC:29797. NUP35.
HPAi HPA018401.
HPA018410.
HPA018441.
MIMi 608140. gene.
neXtProti NX_Q8NFH5.
PharmGKBi PA134861481.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG253574.
HOGENOMi HOG000231921.
HOVERGENi HBG060396.
InParanoidi Q8NFH5.
KOi K14313.
OMAi ANISVMQ.
PhylomeDBi Q8NFH5.
TreeFami TF325369.

Enzyme and pathway databases

Reactomei REACT_115831. ISG15 antiviral mechanism.
REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
REACT_6190. Rev-mediated nuclear export of HIV RNA.
REACT_6804. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_7991. Vpr-mediated nuclear import of PICs.
REACT_9395. Nuclear import of Rev protein.

Miscellaneous databases

GeneWikii NUP35.
GenomeRNAii 129401.
NextBioi 82574.
PROi Q8NFH5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8NFH5.
Bgeei Q8NFH5.
CleanExi HS_NUP35.
Genevestigatori Q8NFH5.

Family and domain databases

InterProi IPR017389. Nucleoporin_NUP53.
IPR007846. RRM_NUP35_dom.
[Graphical view ]
Pfami PF05172. Nup35_RRM. 1 hit.
[Graphical view ]
PIRSFi PIRSF038119. Nucleoporin_NUP53. 1 hit.
PROSITEi PS51472. RRM_NUP35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Proteomic analysis of the mammalian nuclear pore complex."
    Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J.
    J. Cell Biol. 158:915-927(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "Molecular cloning and expression analysis of human mitotic phosphoprotein 44 gene."
    Guo J.H., Yu L.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow and Skin.
  5. "Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex."
    Hawryluk-Gara L.A., Shibuya E.K., Wozniak R.W.
    Mol. Biol. Cell 16:2382-2394(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NUP93, IDENTIFICATION IN A COMPLEX WITH LAMIN B; NUP155 AND NUP205.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells."
    Mansfeld J., Guettinger S., Hawryluk-Gara L.A., Pante N., Mall M., Galy V., Haselmann U., Muehlhaeusser P., Wozniak R.W., Mattaj I.W., Kutay U., Antonin W.
    Mol. Cell 22:93-103(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMEM48.
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-100; THR-106; THR-129; SER-138; THR-265; THR-273; SER-279; THR-280 AND THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-73; SER-100; THR-106; SER-121; THR-273; SER-279 AND THR-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNUP53_HUMAN
AccessioniPrimary (citable) accession number: Q8NFH5
Secondary accession number(s): Q4ZFZ9, Q53S95, Q8TDJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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