Nucleoporin Nup43 - Homo sapiens (Human)

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Names and origin

Protein names

Recommended name:
    Nucleoporin Nup43
Alternative name(s):
    p42
    Nup107-160 subcomplex subunit Nup43

Gene names

Name:

NUP43

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. Ref.5
Subunit structure	Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.
Subcellular location	Kinetochore. Nucleus › nuclear pore complex Ref.1 Ref.4.
Sequence similarities	Contains 6 WD repeats.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis Protein transport Translocation Transport mRNA transport
Cellular component	Kinetochore Nuclear pore complex Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Nup107-160 complex Ref.4 Ref.5 Ref.6 Inferred from direct assay. Source: UniProtKB condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Nucleoporin Nup43

PRO_0000051111

Regions

Repeat

8 – 57

50

WD 1

Repeat

72 – 110

39

WD 2

Repeat

127 – 166

40

WD 3

Repeat

170 – 208

39

WD 4

Repeat

215 – 255

41

WD 5

Repeat

259 – 299

41

WD 6

Amino acid modifications

Modified residue

317

1

Phosphoserine Ref.7

Experimental info

Sequence conflict

247

1

V → A in BAB14536. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8NFH3-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: EE44714B8E5BFB42
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FASTA

380

42,151

        10         20         30         40         50         60 
MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG DFGNLDSDGG 

        70         80         90        100        110        120 
FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF LHHPNNQTLS VNQQWTTAHY 

       130        140        150        160        170        180 
HTGPGSPSYS SAPCTGVVCN NPEIVTVGED GRINLFRADH KEAVRTIDNA DSSTLHAVTF 

       190        200        210        220        230        240 
LRTPEILTVN SIGQLKIWDF RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG 

       250        260        270        280        290        300 
MLSIWDVRQG TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS 

       310        320        330        340        350        360 
LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS LSVNTLDVLG 

       370        380 
PCLVCGTDAE AIYVTRHLFS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteomic analysis of the mammalian nuclear pore complex." Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. J. Cell Biol. 158:915-927(2002) [PubMed: 12196509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 247-380. Tissue: Ovarian carcinoma.
[4]	"The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis." Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J., Sibarita J.-B., Doye V. Mol. Biol. Cell 15:3333-3344(2004) [PubMed: 15146057] [Abstract] Cited for: IDENTIFICATION IN THE NUP107-160 COMPLEX, SUBCELLULAR LOCATION.
[5]	"The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions." Zuccolo M., Alves A., Galy V., Bolhy S., Formstecher E., Racine V., Sibarita J.-B., Fukagawa T., Shiekhattar R., Yen T., Doye V. EMBO J. 26:1853-1864(2007) [PubMed: 17363900] [Abstract] Cited for: FUNCTION OF THE NUP107-160 COMPLEX.
[6]	"Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex." Glavy J.S., Krutchinsky A.N., Cristea I.M., Berke I.C., Boehmer T., Blobel G., Chait B.T. Proc. Natl. Acad. Sci. U.S.A. 104:3811-3816(2007) [PubMed: 17360435] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NUP107-160 COMPLEX.
[7]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF514997 mRNA. Translation: AAM76708.1. BC065028 mRNA. Translation: AAH65028.1. AK023349 mRNA. Translation: BAB14536.1. Different initiation.
IPI	IPI00742943.
RefSeq	NP_942590.1.
UniGene	Hs.510375
3D structure databases
SMR	Q8NFH3. Positions 12-303.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NFH3.
Proteomic databases
PRIDE	Q8NFH3.
Genome annotation databases
Ensembl	ENST00000340413; ENSP00000342262; ENSG00000120253; Homo sapiens. [Genome view]
GeneID	348995.
KEGG	hsa:348995.
UCSC	uc003qmz.1. human.
Organism-specific databases
CTD	348995.
GeneCards	GC06M150137.
H-InvDB	HIX0022549.
HGNC	HGNC:21182. NUP43.
MIM	608141. gene.
PharmGKB	PA134930788.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG713760.
HOVERGEN	Q8NFH3.
InParanoid	Q8NFH3.
OMA	TAAHYHT.
PhylomeDB	Q8NFH3.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_6185. HIV Infection.
Gene expression databases
ArrayExpress	Q8NFH3.
Bgee	Q8NFH3.
CleanEx	HS_NUP43.
Genevestigator	Q8NFH3.
GermOnline	ENSG00000120253. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	99471.
SOURCE	Search...

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Entry information

Entry name

NUP43_HUMAN

Accession

Primary (citable) accession number: Q8NFH3
Secondary accession number(s): Q9H8S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents