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Protein

Nucleoporin Nup43

Gene

NUP43

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis, mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120253-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159227. Transport of the SLBP independent Mature mRNA.
R-HSA-159230. Transport of the SLBP Dependant Mature mRNA.
R-HSA-159231. Transport of Mature mRNA Derived from an Intronless Transcript.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168271. Transport of Ribonucleoproteins into the Host Nucleus.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-191859. snRNP Assembly.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-6784531. tRNA processing in the nucleus.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiQ8NFH3.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin Nup43
Alternative name(s):
Nup107-160 subcomplex subunit Nup43
p42
Gene namesi
Name:NUP43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21182. NUP43.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • nuclear envelope Source: Reactome
  • nuclear pore outer ring Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi348995.
OpenTargetsiENSG00000120253.
PharmGKBiPA134930788.

Polymorphism and mutation databases

BioMutaiNUP43.
DMDMi27923819.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511111 – 380Nucleoporin Nup43Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8NFH3.
MaxQBiQ8NFH3.
PaxDbiQ8NFH3.
PeptideAtlasiQ8NFH3.
PRIDEiQ8NFH3.

PTM databases

iPTMnetiQ8NFH3.
PhosphoSitePlusiQ8NFH3.

Expressioni

Gene expression databases

BgeeiENSG00000120253.
CleanExiHS_NUP43.
ExpressionAtlasiQ8NFH3. baseline and differential.
GenevisibleiQ8NFH3. HS.

Organism-specific databases

HPAiHPA027126.
HPA027291.
HPA028500.

Interactioni

Subunit structurei

Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.2 Publications

Protein-protein interaction databases

BioGridi131544. 52 interactors.
IntActiQ8NFH3. 15 interactors.
MINTiMINT-4535305.
STRINGi9606.ENSP00000342262.

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi10 – 18Combined sources9
Beta strandi31 – 48Combined sources18
Beta strandi66 – 75Combined sources10
Beta strandi77 – 92Combined sources16
Beta strandi97 – 102Combined sources6
Turni104 – 106Combined sources3
Beta strandi109 – 115Combined sources7
Beta strandi118 – 120Combined sources3
Beta strandi134 – 140Combined sources7
Beta strandi143 – 148Combined sources6
Beta strandi151 – 157Combined sources7
Beta strandi164 – 168Combined sources5
Beta strandi175 – 182Combined sources8
Beta strandi185 – 190Combined sources6
Beta strandi193 – 199Combined sources7
Beta strandi203 – 205Combined sources3
Beta strandi208 – 212Combined sources5
Beta strandi214 – 217Combined sources4
Beta strandi220 – 225Combined sources6
Beta strandi232 – 237Combined sources6
Beta strandi242 – 246Combined sources5
Beta strandi254 – 257Combined sources4
Beta strandi264 – 269Combined sources6
Beta strandi276 – 281Combined sources6
Beta strandi286 – 290Combined sources5
Beta strandi341 – 344Combined sources4
Beta strandi353 – 359Combined sources7
Beta strandi362 – 367Combined sources6
Beta strandi370 – 376Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I79X-ray1.75A/B1-380[»]
5A9Qelectron microscopy23.000/9/I/R1-380[»]
ProteinModelPortaliQ8NFH3.
SMRiQ8NFH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 57WD 1Add BLAST50
Repeati72 – 110WD 2Add BLAST39
Repeati127 – 166WD 3Add BLAST40
Repeati170 – 208WD 4Add BLAST39
Repeati215 – 255WD 5Add BLAST41
Repeati259 – 299WD 6Add BLAST41

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4714. Eukaryota.
ENOG410ZTD5. LUCA.
GeneTreeiENSGT00390000004803.
HOGENOMiHOG000007964.
HOVERGENiHBG052696.
InParanoidiQ8NFH3.
KOiK14305.
OMAiQWTAAHY.
OrthoDBiEOG091G0DV7.
PhylomeDBiQ8NFH3.
TreeFamiTF321692.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFH3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG
60 70 80 90 100
DFGNLDSDGG FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF
110 120 130 140 150
LHHPNNQTLS VNQQWTTAHY HTGPGSPSYS SAPCTGVVCN NPEIVTVGED
160 170 180 190 200
GRINLFRADH KEAVRTIDNA DSSTLHAVTF LRTPEILTVN SIGQLKIWDF
210 220 230 240 250
RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG MLSIWDVRQG
260 270 280 290 300
TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS
310 320 330 340 350
LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS
360 370 380
LSVNTLDVLG PCLVCGTDAE AIYVTRHLFS
Length:380
Mass (Da):42,151
Last modified:October 1, 2002 - v1
Checksum:iEE44714B8E5BFB42
GO
Isoform 2 (identifier: Q8NFH3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-264: DNADSSTLHA...SLLKAHEAEM → V

Note: No experimental confirmation available.
Show »
Length:284
Mass (Da):31,512
Checksum:iFF600FBC0ABC96D3
GO

Sequence cautioni

The sequence BAB14536 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247V → A in BAB14536 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056167168 – 264DNADS…HEAEM → V in isoform 2. 1 PublicationAdd BLAST97

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514997 mRNA. Translation: AAM76708.1.
AK023349 mRNA. Translation: BAB14536.1. Different initiation.
AK304244 mRNA. Translation: BAG65112.1.
AL355312 Genomic DNA. No translation available.
BC065028 mRNA. Translation: AAH65028.1.
CCDSiCCDS5218.1. [Q8NFH3-1]
RefSeqiNP_942590.1. NM_198887.2. [Q8NFH3-1]
UniGeneiHs.510375.

Genome annotation databases

EnsembliENST00000340413; ENSP00000342262; ENSG00000120253. [Q8NFH3-1]
ENST00000367404; ENSP00000356374; ENSG00000120253. [Q8NFH3-2]
GeneIDi348995.
KEGGihsa:348995.
UCSCiuc003qmz.5. human. [Q8NFH3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514997 mRNA. Translation: AAM76708.1.
AK023349 mRNA. Translation: BAB14536.1. Different initiation.
AK304244 mRNA. Translation: BAG65112.1.
AL355312 Genomic DNA. No translation available.
BC065028 mRNA. Translation: AAH65028.1.
CCDSiCCDS5218.1. [Q8NFH3-1]
RefSeqiNP_942590.1. NM_198887.2. [Q8NFH3-1]
UniGeneiHs.510375.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I79X-ray1.75A/B1-380[»]
5A9Qelectron microscopy23.000/9/I/R1-380[»]
ProteinModelPortaliQ8NFH3.
SMRiQ8NFH3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131544. 52 interactors.
IntActiQ8NFH3. 15 interactors.
MINTiMINT-4535305.
STRINGi9606.ENSP00000342262.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ8NFH3.
PhosphoSitePlusiQ8NFH3.

Polymorphism and mutation databases

BioMutaiNUP43.
DMDMi27923819.

Proteomic databases

EPDiQ8NFH3.
MaxQBiQ8NFH3.
PaxDbiQ8NFH3.
PeptideAtlasiQ8NFH3.
PRIDEiQ8NFH3.

Protocols and materials databases

DNASUi348995.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340413; ENSP00000342262; ENSG00000120253. [Q8NFH3-1]
ENST00000367404; ENSP00000356374; ENSG00000120253. [Q8NFH3-2]
GeneIDi348995.
KEGGihsa:348995.
UCSCiuc003qmz.5. human. [Q8NFH3-1]

Organism-specific databases

CTDi348995.
DisGeNETi348995.
GeneCardsiNUP43.
HGNCiHGNC:21182. NUP43.
HPAiHPA027126.
HPA027291.
HPA028500.
MIMi608141. gene.
neXtProtiNX_Q8NFH3.
OpenTargetsiENSG00000120253.
PharmGKBiPA134930788.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4714. Eukaryota.
ENOG410ZTD5. LUCA.
GeneTreeiENSGT00390000004803.
HOGENOMiHOG000007964.
HOVERGENiHBG052696.
InParanoidiQ8NFH3.
KOiK14305.
OMAiQWTAAHY.
OrthoDBiEOG091G0DV7.
PhylomeDBiQ8NFH3.
TreeFamiTF321692.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120253-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159227. Transport of the SLBP independent Mature mRNA.
R-HSA-159230. Transport of the SLBP Dependant Mature mRNA.
R-HSA-159231. Transport of Mature mRNA Derived from an Intronless Transcript.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168271. Transport of Ribonucleoproteins into the Host Nucleus.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-191859. snRNP Assembly.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-6784531. tRNA processing in the nucleus.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiQ8NFH3.

Miscellaneous databases

GeneWikiiNUP43.
GenomeRNAii348995.
PROiQ8NFH3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120253.
CleanExiHS_NUP43.
ExpressionAtlasiQ8NFH3. baseline and differential.
GenevisibleiQ8NFH3. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP43_HUMAN
AccessioniPrimary (citable) accession number: Q8NFH3
Secondary accession number(s): B4E2F0, Q9H8S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.