Q8NFH3 (NUP43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoporin Nup43

Alternative name(s):
Nup107-160 subcomplex subunit Nup43
p42

Gene names

Name:

NUP43

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. Ref.5
Subunit structure	Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13. Ref.4 Ref.6
Subcellular location	Chromosome › centromere › kinetochore. Nucleus › nuclear pore complex Ref.1 Ref.4.
Sequence similarities	Contains 6 WD repeats.
Sequence caution	The sequence BAB14536.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis Protein transport Translocation Transport mRNA transport
Cellular component	Centromere Chromosome Kinetochore Nuclear pore complex Nucleus
Domain	Repeat WD repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW cytokine-mediated signaling pathway Traceable author statement. Source: Reactome glucose transport Traceable author statement. Source: Reactome mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW mitotic prometaphase Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of glucose transport Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nuclear pore outer ring Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Nucleoporin Nup43		PRO_0000051111
Regions
	Repeat	8 – 57	50	WD 1
	Repeat	72 – 110	39	WD 2
	Repeat	127 – 166	40	WD 3
	Repeat	170 – 208	39	WD 4
	Repeat	215 – 255	41	WD 5
	Repeat	259 – 299	41	WD 6
Experimental info
	Sequence conflict	247	1	V → A in BAB14536. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8NFH3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: EE44714B8E5BFB42	FASTA	380	42,151
	10 20 30 40 50 60 MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG DFGNLDSDGG 70 80 90 100 110 120 FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF LHHPNNQTLS VNQQWTTAHY 130 140 150 160 170 180 HTGPGSPSYS SAPCTGVVCN NPEIVTVGED GRINLFRADH KEAVRTIDNA DSSTLHAVTF 190 200 210 220 230 240 LRTPEILTVN SIGQLKIWDF RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG 250 260 270 280 290 300 MLSIWDVRQG TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS 310 320 330 340 350 360 LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS LSVNTLDVLG 370 380 PCLVCGTDAE AIYVTRHLFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteomic analysis of the mammalian nuclear pore complex." Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. J. Cell Biol. 158:915-927(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 247-380. Tissue: Ovarian carcinoma.
[4]	"The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis." Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J., Sibarita J.-B., Doye V. Mol. Biol. Cell 15:3333-3344(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE NUP107-160 COMPLEX, SUBCELLULAR LOCATION.
[5]	"The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions." Zuccolo M., Alves A., Galy V., Bolhy S., Formstecher E., Racine V., Sibarita J.-B., Fukagawa T., Shiekhattar R., Yen T., Doye V. EMBO J. 26:1853-1864(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NUP107-160 COMPLEX.
[6]	"Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex." Glavy J.S., Krutchinsky A.N., Cristea I.M., Berke I.C., Boehmer T., Blobel G., Chait B.T. Proc. Natl. Acad. Sci. U.S.A. 104:3811-3816(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NUP107-160 COMPLEX.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF514997 mRNA. Translation: AAM76708.1.
BC065028 mRNA. Translation: AAH65028.1.
AK023349 mRNA. Translation: BAB14536.1. Different initiation.

IPI

IPI00742943.

RefSeq

NP_942590.1. NM_198887.1.

UniGene

Hs.510375.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4I79	X-ray	1.75	A/B	1-380	[»]

ProteinModelPortal

Q8NFH3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8NFH3. 3 interactions.

STRING

9606.ENSP00000342262.

PTM databases

PhosphoSite

Q8NFH3.

Polymorphism databases

DMDM

27923819.

Proteomic databases

PaxDb

Q8NFH3.

PRIDE

Q8NFH3.

Protocols and materials databases

DNASU

348995.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000340413; ENSP00000342262; ENSG00000120253.
ENST00000460354; ENSP00000432401; ENSG00000120253.

GeneID

348995.

KEGG

hsa:348995.

UCSC

uc003qmz.3. human.

Organism-specific databases

CTD

348995.

GeneCards

GC06M150087.

HGNC

HGNC:21182. NUP43.

HPA

HPA027126.
HPA027291.
HPA028500.

MIM

608141. gene.

neXtProt

NX_Q8NFH3.

PharmGKB

PA134930788.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG270547.

HOGENOM

HOG000007964.

HOVERGEN

HBG052696.

InParanoid

Q8NFH3.

KO

K14305.

OMA

QWTAAHY.

PhylomeDB

Q8NFH3.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.
REACT_115566. Cell Cycle.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.
REACT_21300. Mitotic M-M/G1 phases.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

Q8NFH3.

Bgee

Q8NFH3.

CleanEx

HS_NUP43.

Genevestigator

Q8NFH3.

GermOnline

ENSG00000120253. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 1 hit.

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF00400. WD40. 2 hits.
[Graphical view]

SMART

SM00320. WD40. 5 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

348995.

NextBio

99471.

SOURCE

Search...

Entry information

Entry name

NUP43_HUMAN

Accession

Primary (citable) accession number: Q8NFH3
Secondary accession number(s): Q9H8S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.