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Protein

FAD synthase

Gene

FLAD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.

Catalytic activityi

ATP + FMN = diphosphate + FAD.1 Publication

Cofactori

Mg2+2 Publications

Kineticsi

  1. KM=1.5 µM for FMN2 Publications
  2. KM=0.36 µM for FMN (isoform 2)2 Publications
  1. Vmax=6.1 nmol/min/mg enzyme2 Publications
  2. Vmax=3.9 nmol/min/mg enzyme (isoform 2)2 Publications

Pathwayi: FAD biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FAD from FMN.
Proteins known to be involved in this subpathway in this organism are:
  1. FAD synthase (FLAD1)
This subpathway is part of the pathway FAD biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FAD from FMN, the pathway FAD biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS08520-MONOMER.
ZFISH:HS08520-MONOMER.
BRENDAi2.7.7.2. 2681.
ReactomeiR-HSA-196843. Vitamin B2 (riboflavin) metabolism.
SABIO-RKQ8NFF5.
UniPathwayiUPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD synthase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FMN adenylyltransferase
Flavin adenine dinucleotide synthase
Including the following 2 domains:
Molybdenum cofactor biosynthesis protein-like region
FAD synthase region
Gene namesi
Name:FLAD1
ORF Names:PP591
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24671. FLAD1.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi80308.
OpenTargetsiENSG00000160688.
PharmGKBiPA142671759.

Polymorphism and mutation databases

BioMutaiFLAD1.
DMDMi74751275.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000030273718 – 587FAD synthaseAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei106PhosphoserineCombined sources1
Modified residuei378N6-acetyllysine; alternateBy similarity1
Modified residuei378N6-succinyllysine; alternateBy similarity1
Modified residuei563PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8NFF5.
MaxQBiQ8NFF5.
PaxDbiQ8NFF5.
PeptideAtlasiQ8NFF5.
PRIDEiQ8NFF5.

PTM databases

iPTMnetiQ8NFF5.
PhosphoSitePlusiQ8NFF5.

Expressioni

Gene expression databases

BgeeiENSG00000160688.
CleanExiHS_FLAD1.
ExpressionAtlasiQ8NFF5. baseline and differential.
GenevisibleiQ8NFF5. HS.

Organism-specific databases

HPAiHPA028476.
HPA028486.
HPA028563.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RELQ048643EBI-742815,EBI-307352
SDCBPO005603EBI-742815,EBI-727004
TCF4P158843EBI-742815,EBI-533224

Protein-protein interaction databases

BioGridi123221. 29 interactors.
IntActiQ8NFF5. 22 interactors.
MINTiMINT-3042841.
STRINGi9606.ENSP00000292180.

Structurei

3D structure databases

ProteinModelPortaliQ8NFF5.
SMRiQ8NFF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 205Molybdenum cofactor biosynthesis protein-likeAdd BLAST92
Regioni398 – 555FAD synthaseAdd BLAST158

Domaini

The molybdenum cofactor biosynthesis protein-like region may not be functional.

Sequence similaritiesi

In the N-terminal section; belongs to the MoaB/Mog family.Curated
In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IMG8. Eukaryota.
KOG2644. Eukaryota.
COG0175. LUCA.
COG1058. LUCA.
GeneTreeiENSGT00390000007266.
HOVERGENiHBG058211.
InParanoidiQ8NFF5.
KOiK00953.
OMAiCVLENVY.
OrthoDBiEOG091G0PRM.
PhylomeDBiQ8NFF5.
TreeFamiTF314056.

Family and domain databases

CDDicd00885. cinA. 1 hit.
cd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.980.10. 1 hit.
InterProiIPR012183. FAD_synth_MoaB/Mog-bd.
IPR001453. MoaB/Mog_dom.
IPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00994. MoCF_biosynth. 1 hit.
PF01507. PAPS_reduct. 2 hits.
[Graphical view]
PIRSFiPIRSF036620. MPTbdFAD. 1 hit.
SMARTiSM00852. MoCF_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF53218. SSF53218. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFF5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGWDLGTRLF QRQEQRSRLS RIWLEKTRVF LEGSTRTPAL PHCLFWLLQV
60 70 80 90 100
PSTQDPLFPG YGPQCPVDLA GPPCLRPLFG GLGGYWRALQ RGREGRTMTS
110 120 130 140 150
RASELSPGRS VTAGIIIVGD EILKGHTQDT NTFFLCRTLR SLGVQVCRVS
160 170 180 190 200
VVPDEVATIA AEVTSFSNRF THVLTAGGIG PTHDDVTFEA VAQAFGDELK
210 220 230 240 250
PHPKLEAATK ALGGEGWEKL SLVPSSARLH YGTDPCTGQP FRFPLVSVRN
260 270 280 290 300
VYLFPGIPEL LRRVLEGMKG LFQNPAVQFH SKELYVAADE ASIAPILAEA
310 320 330 340 350
QAHFGRRLGL GSYPDWGSNY YQVKLTLDSE EEGPLEECLA YLTARLPQGS
360 370 380 390 400
LVPYMPNAVE QASEAVYKLA ESGSSLGKKV AGALQTIETS LAQYSLTQLC
410 420 430 440 450
VGFNGGKDCT ALLHLFHAAV QRKLPDVPNP LQILYIRSIS PFPELEQFLQ
460 470 480 490 500
DTIKRYNLQM LEAEGSMKQA LGELQARHPQ LEAVLMGTRR TDPYSCSLCP
510 520 530 540 550
FSPTDPGWPA FMRINPLLDW TYRDIWDFLR QLFVPYCILY DRGYTSLGSR
560 570 580
ENTVRNPALK CLSPGGHPTY RPAYLLENEE EERNSRT
Length:587
Mass (Da):65,266
Last modified:October 1, 2002 - v1
Checksum:iF95918B15D9D8106
GO
Isoform 2 (identifier: Q8NFF5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.

Show »
Length:490
Mass (Da):54,188
Checksum:i633D7949AB88E059
GO
Isoform 3 (identifier: Q8NFF5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.
     544-587: Missing.

Show »
Length:446
Mass (Da):49,198
Checksum:i2983E4FA84020CDF
GO
Isoform 4 (identifier: Q8NFF5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     31-124: LEGSTRTPAL...IIIVGDEILK → MQPSSSTPPLHPYSTDGLIFPFNPQ
     374-393: SSLGKKVAGALQTIETSLAQ → RDLMEEGHYAQSHWWHPRSQ
     394-587: Missing.

Show »
Length:294
Mass (Da):32,369
Checksum:i5CEF3426F7FEDEF5
GO
Isoform 5 (identifier: Q8NFF5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     31-124: LEGSTRTPAL...IIIVGDEILK → MQPSSSTPPLHPYSTDGLIFPFNPQ
     374-437: SSLGKKVAGA...PNPLQILYIR → NYLMFQTPSR...WMGPFPGQQG
     438-587: Missing.

Show »
Length:338
Mass (Da):36,865
Checksum:i2BB52386763E2A9B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0279471 – 97Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST97
Alternative sequenceiVSP_0279481 – 30Missing in isoform 4 and isoform 5. 2 PublicationsAdd BLAST30
Alternative sequenceiVSP_02794931 – 124LEGST…DEILK → MQPSSSTPPLHPYSTDGLIF PFNPQ in isoform 4 and isoform 5. 2 PublicationsAdd BLAST94
Alternative sequenceiVSP_027950374 – 437SSLGK…ILYIR → NYLMFQTPSRSCISAASPLS LSWNSFYRTLSREQAIPENQ IASPPSEAKGAEEPWMGPFP GQQG in isoform 5. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_027951374 – 393SSLGK…TSLAQ → RDLMEEGHYAQSHWWHPRSQ in isoform 4. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_027952394 – 587Missing in isoform 4. 1 PublicationAdd BLAST194
Alternative sequenceiVSP_027953438 – 587Missing in isoform 5. 1 PublicationAdd BLAST150
Alternative sequenceiVSP_027954544 – 587Missing in isoform 3. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ458779 mRNA. Translation: ABE65383.1.
AF481877 mRNA. Translation: AAO49318.1.
AF520568 mRNA. Translation: AAM77338.1.
AF218022 mRNA. Translation: AAG17264.1.
AL451085 Genomic DNA. Translation: CAI13259.1.
AL451085 Genomic DNA. Translation: CAI13260.1.
AL451085 Genomic DNA. Translation: CAI13261.1.
AL451085 Genomic DNA. Translation: CAI13262.1.
CH471121 Genomic DNA. Translation: EAW53154.1.
CH471121 Genomic DNA. Translation: EAW53155.1.
CH471121 Genomic DNA. Translation: EAW53158.1.
CH471121 Genomic DNA. Translation: EAW53159.1.
BC011378 mRNA. Translation: AAH11378.1.
BC014012 mRNA. Translation: AAH14012.2.
BC020253 mRNA. Translation: AAH20253.1.
BC021096 mRNA. Translation: AAH21096.2.
BC032323 mRNA. Translation: AAH32323.1.
U79241 mRNA. Translation: AAB50199.1.
CCDSiCCDS1078.1. [Q8NFF5-1]
CCDS1079.1. [Q8NFF5-2]
CCDS53371.1. [Q8NFF5-3]
CCDS53372.1. [Q8NFF5-4]
RefSeqiNP_001171820.1. NM_001184891.1. [Q8NFF5-3]
NP_001171821.1. NM_001184892.1. [Q8NFF5-4]
NP_079483.3. NM_025207.4. [Q8NFF5-1]
NP_958800.1. NM_201398.2. [Q8NFF5-2]
UniGeneiHs.118666.

Genome annotation databases

EnsembliENST00000292180; ENSP00000292180; ENSG00000160688. [Q8NFF5-1]
ENST00000315144; ENSP00000317296; ENSG00000160688. [Q8NFF5-2]
ENST00000368431; ENSP00000357416; ENSG00000160688. [Q8NFF5-4]
ENST00000368432; ENSP00000357417; ENSG00000160688. [Q8NFF5-3]
GeneIDi80308.
KEGGihsa:80308.
UCSCiuc001fgc.4. human. [Q8NFF5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ458779 mRNA. Translation: ABE65383.1.
AF481877 mRNA. Translation: AAO49318.1.
AF520568 mRNA. Translation: AAM77338.1.
AF218022 mRNA. Translation: AAG17264.1.
AL451085 Genomic DNA. Translation: CAI13259.1.
AL451085 Genomic DNA. Translation: CAI13260.1.
AL451085 Genomic DNA. Translation: CAI13261.1.
AL451085 Genomic DNA. Translation: CAI13262.1.
CH471121 Genomic DNA. Translation: EAW53154.1.
CH471121 Genomic DNA. Translation: EAW53155.1.
CH471121 Genomic DNA. Translation: EAW53158.1.
CH471121 Genomic DNA. Translation: EAW53159.1.
BC011378 mRNA. Translation: AAH11378.1.
BC014012 mRNA. Translation: AAH14012.2.
BC020253 mRNA. Translation: AAH20253.1.
BC021096 mRNA. Translation: AAH21096.2.
BC032323 mRNA. Translation: AAH32323.1.
U79241 mRNA. Translation: AAB50199.1.
CCDSiCCDS1078.1. [Q8NFF5-1]
CCDS1079.1. [Q8NFF5-2]
CCDS53371.1. [Q8NFF5-3]
CCDS53372.1. [Q8NFF5-4]
RefSeqiNP_001171820.1. NM_001184891.1. [Q8NFF5-3]
NP_001171821.1. NM_001184892.1. [Q8NFF5-4]
NP_079483.3. NM_025207.4. [Q8NFF5-1]
NP_958800.1. NM_201398.2. [Q8NFF5-2]
UniGeneiHs.118666.

3D structure databases

ProteinModelPortaliQ8NFF5.
SMRiQ8NFF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123221. 29 interactors.
IntActiQ8NFF5. 22 interactors.
MINTiMINT-3042841.
STRINGi9606.ENSP00000292180.

PTM databases

iPTMnetiQ8NFF5.
PhosphoSitePlusiQ8NFF5.

Polymorphism and mutation databases

BioMutaiFLAD1.
DMDMi74751275.

Proteomic databases

EPDiQ8NFF5.
MaxQBiQ8NFF5.
PaxDbiQ8NFF5.
PeptideAtlasiQ8NFF5.
PRIDEiQ8NFF5.

Protocols and materials databases

DNASUi80308.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292180; ENSP00000292180; ENSG00000160688. [Q8NFF5-1]
ENST00000315144; ENSP00000317296; ENSG00000160688. [Q8NFF5-2]
ENST00000368431; ENSP00000357416; ENSG00000160688. [Q8NFF5-4]
ENST00000368432; ENSP00000357417; ENSG00000160688. [Q8NFF5-3]
GeneIDi80308.
KEGGihsa:80308.
UCSCiuc001fgc.4. human. [Q8NFF5-1]

Organism-specific databases

CTDi80308.
DisGeNETi80308.
GeneCardsiFLAD1.
HGNCiHGNC:24671. FLAD1.
HPAiHPA028476.
HPA028486.
HPA028563.
MIMi610595. gene.
neXtProtiNX_Q8NFF5.
OpenTargetsiENSG00000160688.
PharmGKBiPA142671759.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IMG8. Eukaryota.
KOG2644. Eukaryota.
COG0175. LUCA.
COG1058. LUCA.
GeneTreeiENSGT00390000007266.
HOVERGENiHBG058211.
InParanoidiQ8NFF5.
KOiK00953.
OMAiCVLENVY.
OrthoDBiEOG091G0PRM.
PhylomeDBiQ8NFF5.
TreeFamiTF314056.

Enzyme and pathway databases

UniPathwayiUPA00277; UER00407.
BioCyciMetaCyc:HS08520-MONOMER.
ZFISH:HS08520-MONOMER.
BRENDAi2.7.7.2. 2681.
ReactomeiR-HSA-196843. Vitamin B2 (riboflavin) metabolism.
SABIO-RKQ8NFF5.

Miscellaneous databases

ChiTaRSiFLAD1. human.
GenomeRNAii80308.
PROiQ8NFF5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160688.
CleanExiHS_FLAD1.
ExpressionAtlasiQ8NFF5. baseline and differential.
GenevisibleiQ8NFF5. HS.

Family and domain databases

CDDicd00885. cinA. 1 hit.
cd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.980.10. 1 hit.
InterProiIPR012183. FAD_synth_MoaB/Mog-bd.
IPR001453. MoaB/Mog_dom.
IPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00994. MoCF_biosynth. 1 hit.
PF01507. PAPS_reduct. 2 hits.
[Graphical view]
PIRSFiPIRSF036620. MPTbdFAD. 1 hit.
SMARTiSM00852. MoCF_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF53218. SSF53218. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFAD1_HUMAN
AccessioniPrimary (citable) accession number: Q8NFF5
Secondary accession number(s): Q8N5J1
, Q8N686, Q8WU93, Q8WUJ4, Q96CR8, Q99764, Q9HBN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.