SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8NFA2

- NOXO1_HUMAN

UniProt

Q8NFA2 - NOXO1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADPH oxidase organizer 1

Gene
NOXO1, P41NOX, SH3PXD5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.8 Publications

GO - Molecular functioni

  1. enzyme binding Source: BHF-UCL
  2. identical protein binding Source: IntAct
  3. phosphatidylinositol binding Source: InterPro
  4. phospholipid binding Source: BHF-UCL
  5. protein binding Source: IntAct
  6. superoxide-generating NADPH oxidase activator activity Source: BHF-UCL

GO - Biological processi

  1. extracellular matrix disassembly Source: UniProtKB
  2. positive regulation of catalytic activity Source: GOC
  3. regulation of hydrogen peroxide metabolic process Source: BHF-UCL
  4. regulation of respiratory burst Source: BHF-UCL
  5. superoxide metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase organizer 1
Alternative name(s):
NADPH oxidase regulatory protein
Nox organizer 1
Nox-organizing protein 1
SH3 and PX domain-containing protein 5
Gene namesi
Name:NOXO1
Synonyms:P41NOX, SH3PXD5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:19404. NOXO1.

Subcellular locationi

Isoform 3 : Cell membrane; Peripheral membrane protein; Cytoplasmic side
Note: Isoform 3 associates with the plasma membrane in a lipid-dependent manner.1 Publication

GO - Cellular componenti

  1. NADPH oxidase complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401R → Q: Loss of ability to activate NOX1 associated with loss of lipid-binding and plasma membrane localization. 1 Publication
Mutagenesisi202 – 2021W → R: Loss of ability to activate NOX3 and interact with CYBA. Induces interaction with NOXA1 in vitro. 2 Publications
Mutagenesisi274 – 2741W → R: Induces interaction with NOXA1 in vitro. 1 Publication
Mutagenesisi332 – 3321P → A: Loss of intramolecular interaction. 1 Publication
Mutagenesisi334 – 3341R → A: Loss of intramolecular interaction. 1 Publication

Organism-specific databases

PharmGKBiPA134896072.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376NADPH oxidase organizer 1PRO_0000227594Add
BLAST

Proteomic databases

PaxDbiQ8NFA2.
PRIDEiQ8NFA2.

PTM databases

PhosphoSiteiQ8NFA2.

Expressioni

Tissue specificityi

Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.3 Publications

Developmental stagei

Isoform 3 is expressed in fetal liver.1 Publication

Gene expression databases

ArrayExpressiQ8NFA2.
BgeeiQ8NFA2.
CleanExiHS_NOXO1.
GenevestigatoriQ8NFA2.

Interactioni

Subunit structurei

Interacts with NOX1, NOXA1, CYBA/p22phox and NCF2/p67phox. Interacts with SH3PXD2A and SH3PXD2B.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7130806,EBI-7130806
CYBAP134986EBI-7130806,EBI-986058
NOXA1Q86UR16EBI-7130806,EBI-949814

Protein-protein interaction databases

IntActiQ8NFA2. 2 interactions.
MINTiMINT-7147796.
STRINGi9606.ENSP00000380450.

Structurei

Secondary structure

1
376
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 1610
Beta strandi19 – 213
Beta strandi23 – 308
Beta strandi35 – 417
Helixi42 – 5514
Helixi57 – 604
Beta strandi61 – 633
Helixi89 – 11022
Helixi112 – 1165
Helixi118 – 1247
Helixi128 – 1314
Beta strandi132 – 1343

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L73NMR-A1-149[»]
ProteinModelPortaliQ8NFA2.
SMRiQ8NFA2. Positions 2-149, 192-302.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 131131PXAdd
BLAST
Domaini163 – 22563SH3 1Add
BLAST
Domaini237 – 29660SH3 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni328 – 33710Proline-rich region; mediates mutually exclusive interactions with itself and NOXA1

Domaini

The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.2 Publications
The SH3 domains mediate interaction with CYBA/p22phox. Also mediates intramolecular interaction with the proline-rich region.2 Publications

Sequence similaritiesi

Contains 2 SH3 domains.

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG150477.
HOGENOMiHOG000113833.
HOVERGENiHBG082053.
InParanoidiQ8NFA2.
KOiK17934.
OMAiTFVRRSW.
OrthoDBiEOG70GMG8.
PhylomeDBiQ8NFA2.
TreeFamiTF329347.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8NFA2-1) [UniParc]FASTAAdd to Basket

Also known as: NOXO1gamma

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGPRYPVSV QGAALVQIKR LQTFAFSVRW SDGSDTFVRR SWDEFRQLKK    50
TLKETFPVEA GLLRRSDRVL PKLLGQASLD APLLGRVGRT SRGLARLQLL 100
ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQP 150
LSRAAGRLSI HSLEAQSLRC LQPFCTQDTR DRPFQAQAQE SLDVLLRHPS 200
GWWLVENEDR QTAWFPAPYL EEAAPGQGRE GGPSLGSSGP QFCASRAYES 250
SRADELSVPA GARVRVLETS DRGWWLCRYG DRAGLLPAVL LRPEGLGALL 300
SGTGFRGGDD PAGEARGFPE PSQATAPPPT VPTRPSPGAI QSRCCTVTRR 350
ALERRPRRQG RPRGCVDSVP HPTTEQ 376
Length:376
Mass (Da):41,253
Last modified:October 1, 2002 - v1
Checksum:i2E9525A8BADDB360
GO
Isoform 2 (identifier: Q8NFA2-2) [UniParc]FASTAAdd to Basket

Also known as: NOXO1delta

The sequence of this isoform differs from the canonical sequence as follows:
     49-49: Missing.

Show »
Length:375
Mass (Da):41,125
Checksum:i85E41025A14AEA80
GO
Isoform 3 (identifier: Q8NFA2-3) [UniParc]FASTAAdd to Basket

Also known as: NOXO1beta

The sequence of this isoform differs from the canonical sequence as follows:
     74-78: Missing.

Show »
Length:371
Mass (Da):40,796
Checksum:i39E13C3FF491797E
GO
Isoform 4 (identifier: Q8NFA2-4) [UniParc]FASTAAdd to Basket

Also known as: NOXO1alpha

The sequence of this isoform differs from the canonical sequence as follows:
     49-49: Missing.
     74-78: Missing.

Show »
Length:370
Mass (Da):40,668
Checksum:i1EB8DCE8BC50551F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei49 – 491Missing in isoform 2 and isoform 4. VSP_017560
Alternative sequencei74 – 785Missing in isoform 3 and isoform 4. VSP_017561

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF539796 mRNA. Translation: AAN75141.1.
AY255768 mRNA. Translation: AAP13479.1.
AB097667 mRNA. Translation: BAC76711.1.
AF532983 mRNA. Translation: AAM97925.1.
AF532984 mRNA. Translation: AAM97926.1.
AF532985 mRNA. Translation: AAM97927.1.
AY191359 mRNA. Translation: AAO38665.1.
BC015917 mRNA. Translation: AAH15917.1.
CCDSiCCDS10454.1. [Q8NFA2-4]
CCDS10455.1. [Q8NFA2-3]
CCDS42101.1. [Q8NFA2-1]
CCDS58406.1. [Q8NFA2-2]
RefSeqiNP_001254650.1. NM_001267721.1. [Q8NFA2-2]
NP_653204.1. NM_144603.3. [Q8NFA2-4]
NP_751907.1. NM_172167.2. [Q8NFA2-3]
NP_751908.1. NM_172168.2. [Q8NFA2-1]
XP_005255156.1. XM_005255099.2. [Q8NFA2-1]
UniGeneiHs.191762.

Genome annotation databases

EnsembliENST00000354249; ENSP00000346195; ENSG00000196408. [Q8NFA2-4]
ENST00000356120; ENSP00000348435; ENSG00000196408. [Q8NFA2-3]
ENST00000397280; ENSP00000380450; ENSG00000196408. [Q8NFA2-1]
ENST00000566005; ENSP00000456800; ENSG00000196408. [Q8NFA2-2]
GeneIDi124056.
KEGGihsa:124056.
UCSCiuc002cnx.4. human. [Q8NFA2-1]
uc002cny.4. human. [Q8NFA2-3]
uc002cnz.4. human. [Q8NFA2-4]
uc002coa.4. human. [Q8NFA2-2]

Polymorphism databases

DMDMi74751269.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF539796 mRNA. Translation: AAN75141.1 .
AY255768 mRNA. Translation: AAP13479.1 .
AB097667 mRNA. Translation: BAC76711.1 .
AF532983 mRNA. Translation: AAM97925.1 .
AF532984 mRNA. Translation: AAM97926.1 .
AF532985 mRNA. Translation: AAM97927.1 .
AY191359 mRNA. Translation: AAO38665.1 .
BC015917 mRNA. Translation: AAH15917.1 .
CCDSi CCDS10454.1. [Q8NFA2-4 ]
CCDS10455.1. [Q8NFA2-3 ]
CCDS42101.1. [Q8NFA2-1 ]
CCDS58406.1. [Q8NFA2-2 ]
RefSeqi NP_001254650.1. NM_001267721.1. [Q8NFA2-2 ]
NP_653204.1. NM_144603.3. [Q8NFA2-4 ]
NP_751907.1. NM_172167.2. [Q8NFA2-3 ]
NP_751908.1. NM_172168.2. [Q8NFA2-1 ]
XP_005255156.1. XM_005255099.2. [Q8NFA2-1 ]
UniGenei Hs.191762.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2L73 NMR - A 1-149 [» ]
ProteinModelPortali Q8NFA2.
SMRi Q8NFA2. Positions 2-149, 192-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8NFA2. 2 interactions.
MINTi MINT-7147796.
STRINGi 9606.ENSP00000380450.

PTM databases

PhosphoSitei Q8NFA2.

Polymorphism databases

DMDMi 74751269.

Proteomic databases

PaxDbi Q8NFA2.
PRIDEi Q8NFA2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000354249 ; ENSP00000346195 ; ENSG00000196408 . [Q8NFA2-4 ]
ENST00000356120 ; ENSP00000348435 ; ENSG00000196408 . [Q8NFA2-3 ]
ENST00000397280 ; ENSP00000380450 ; ENSG00000196408 . [Q8NFA2-1 ]
ENST00000566005 ; ENSP00000456800 ; ENSG00000196408 . [Q8NFA2-2 ]
GeneIDi 124056.
KEGGi hsa:124056.
UCSCi uc002cnx.4. human. [Q8NFA2-1 ]
uc002cny.4. human. [Q8NFA2-3 ]
uc002cnz.4. human. [Q8NFA2-4 ]
uc002coa.4. human. [Q8NFA2-2 ]

Organism-specific databases

CTDi 124056.
GeneCardsi GC16M002028.
HGNCi HGNC:19404. NOXO1.
MIMi 611256. gene.
neXtProti NX_Q8NFA2.
PharmGKBi PA134896072.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG150477.
HOGENOMi HOG000113833.
HOVERGENi HBG082053.
InParanoidi Q8NFA2.
KOi K17934.
OMAi TFVRRSW.
OrthoDBi EOG70GMG8.
PhylomeDBi Q8NFA2.
TreeFami TF329347.

Miscellaneous databases

GeneWikii NOXO1.
GenomeRNAii 124056.
NextBioi 81216.
PROi Q8NFA2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8NFA2.
Bgeei Q8NFA2.
CleanExi HS_NOXO1.
Genevestigatori Q8NFA2.

Family and domain databases

Gene3Di 3.30.1520.10. 1 hit.
InterProi IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 2 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEi PS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel proteins activate superoxide generation by the NADPH oxidase NOX1."
    Banfi B., Clark R.A., Steger K., Krause K.-H.
    J. Biol. Chem. 278:3510-3513(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  2. "Proteins homologous to p47phox and p67phox support superoxide production by NAD(P)H oxidase 1 in colon epithelial cells."
    Geiszt M., Lekstrom K., Witta J., Leto T.L.
    J. Biol. Chem. 278:20006-20012(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Colon.
  3. "Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases."
    Takeya R., Ueno N., Kami K., Taura M., Kohjima M., Izaki T., Nunoi H., Sumimoto H.
    J. Biol. Chem. 278:25234-25246(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH NOXA1; CYBA AND NCF2, DOMAIN.
  4. "NOXO1, regulation of lipid binding, localization, and activation of Nox1 by the Phox homology (PX) domain."
    Cheng G., Lambeth J.D.
    J. Biol. Chem. 279:4737-4742(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, MUTAGENESIS OF ARG-40, DOMAIN, SUBCELLULAR LOCATION.
    Tissue: Colon.
  5. "Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3."
    Cheng G., Lambeth J.D.
    Gene 356:118-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 4), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Colon and Testis.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Colon.
  7. Cited for: FUNCTION, TISSUE SPECIFICITY.
  8. "The NADPH oxidase Nox3 constitutively produces superoxide in a p22phox-dependent manner: its regulation by oxidase organizers and activators."
    Ueno N., Takeya R., Miyano K., Kikuchi H., Sumimoto H.
    J. Biol. Chem. 280:23328-23339(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-202.
  9. "Molecular interaction of NADPH oxidase 1 with betaPix and Nox Organizer 1."
    Park H.S., Park D., Bae Y.S.
    Biochem. Biophys. Res. Commun. 339:985-990(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NOX1.
  10. "Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1 (Noxo1)."
    Yamamoto A., Kami K., Takeya R., Sumimoto H.
    Biochem. Biophys. Res. Commun. 352:560-565(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CYBA AND NOXA1, MUTAGENESIS OF TRP-202; TRP-274; PRO-332 AND ARG-334.
  11. "Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity."
    Gianni D., Diaz B., Taulet N., Fowler B., Courtneidge S.A., Bokoch G.M.
    Sci. Signal. 2:RA54-RA54(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Direct interaction between Tks proteins and the N-terminal proline-rich region (PRR) of NoxA1 mediates Nox1-dependent ROS generation."
    Gianni D., Dermardirossian C., Bokoch G.M.
    Eur. J. Cell Biol. 90:164-171(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOXA1; SH3PXD2A AND SH3PXD2B.

Entry informationi

Entry nameiNOXO1_HUMAN
AccessioniPrimary (citable) accession number: Q8NFA2
Secondary accession number(s): Q86YM1, Q8NFA3, Q96B73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi