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Protein

Ubiquitin carboxyl-terminal hydrolase 32

Gene

USP32

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei743NucleophilePROSITE-ProRule annotation1
Active sitei1526Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi241 – 2521PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi277 – 2882PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • thiol-dependent ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  • protein deubiquitination Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 32 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 32
Renal carcinoma antigen NY-REN-60
Ubiquitin thioesterase 32
Ubiquitin-specific-processing protease 32
Gene namesi
Name:USP32
Synonyms:USP10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000170832.12
HGNCiHGNC:19143 USP32
MIMi607740 gene
neXtProtiNX_Q8NFA0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84669
OpenTargetsiENSG00000170832
PharmGKBiPA134982542

Polymorphism and mutation databases

BioMutaiUSP32
DMDMi47606649

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806631 – 1601Ubiquitin carboxyl-terminal hydrolase 32Add BLAST1601
PropeptideiPRO_00003966581602 – 1604Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1173PhosphotyrosineCombined sources1
Modified residuei1350PhosphoserineCombined sources1
Modified residuei1372PhosphoserineCombined sources1
Modified residuei1376PhosphoserineCombined sources1
Modified residuei1454PhosphoserineCombined sources1
Modified residuei1588PhosphoserineCombined sources1
Modified residuei1601Cysteine methyl esterSequence analysis1
Lipidationi1601S-farnesyl cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ8NFA0
MaxQBiQ8NFA0
PaxDbiQ8NFA0
PeptideAtlasiQ8NFA0
PRIDEiQ8NFA0

PTM databases

iPTMnetiQ8NFA0
PhosphoSitePlusiQ8NFA0
SwissPalmiQ8NFA0

Expressioni

Gene expression databases

BgeeiENSG00000170832
CleanExiHS_USP10
HS_USP32
ExpressionAtlasiQ8NFA0 baseline and differential
GenevisibleiQ8NFA0 HS

Organism-specific databases

HPAiHPA044365

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ZDHHC17Q8IUH52EBI-2511075,EBI-524753

Protein-protein interaction databases

BioGridi124189, 44 interactors
DIPiDIP-53613N
IntActiQ8NFA0, 31 interactors
MINTiQ8NFA0
STRINGi9606.ENSP00000300896

Structurei

3D structure databases

ProteinModelPortaliQ8NFA0
SMRiQ8NFA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 126EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini228 – 263EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini264 – 299EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini369 – 585DUSPPROSITE-ProRule annotationAdd BLAST217
Domaini734 – 1567USPAdd BLAST834

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044 Eukaryota
KOG1870 Eukaryota
COG5560 LUCA
GeneTreeiENSGT00670000097750
HOGENOMiHOG000202412
HOVERGENiHBG057958
InParanoidiQ8NFA0
KOiK11837
OMAiSDNNNQC
OrthoDBiEOG091G01VD
PhylomeDBiQ8NFA0
TreeFamiTF324190

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR035927 DUSP-like_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR006615 Pept_C19_DUSP
IPR001394 Peptidase_C19_UCH
IPR028135 Ub_USP-typ
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF06337 DUSP, 1 hit
PF13202 EF-hand_5, 2 hits
PF14836 Ubiquitin_3, 1 hit
PF00443 UCH, 1 hit
SMARTiView protein in SMART
SM00695 DUSP, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF143791 SSF143791, 2 hits
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS51283 DUSP, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFA0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE
60 70 80 90 100
VLGDGVPPKV AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG KDEEKAKYIF
110 120 130 140 150
SLFSSESGNY VIREEMERML HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL
160 170 180 190 200
FLNKDAFTFS RWLLSGGVYV TLTDDSDTPT FYQTLAGVTH LEESDIIDLE
210 220 230 240 250
KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF DENRDNHIDF
260 270 280 290 300
KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRVELR DMVVALLEVW
310 320 330 340 350
KDNRTDDIPE LHMDLSDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN
360 370 380 390 400
EFLNLLFQVC HIVLGLRPAT PEEEGQIIRG WLERESRYGL QAGHNWFIIS
410 420 430 440 450
MQWWQQWKEY VKYDANPVVI EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS
460 470 480 490 500
LSYVNTTEEK FSDNISTASE ASETAGSGFL YSATPGADVC FARQHNTSDN
510 520 530 540 550
NNQCLLGANG NILLHLNPQK PGAIDNQPLV TQEPVKATSL TLEGGRLKRT
560 570 580 590 600
PQLIHGRDYE MVPEPVWRAL YHWYGANLAL PRPVIKNSKT DIPELELFPR
610 620 630 640 650
YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA PLKRVLAYTG CFSRMQTIKE
660 670 680 690 700
IHEYLSQRLR IKEEDMRLWL YNSENYLTLL DDEDHKLEYL KIQDEQHLVI
710 720 730 740 750
EVRNKDMSWP EEMSFIANSS KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ
760 770 780 790 800
CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK GHMAKCYGDL VQELWSGTQK
810 820 830 840 850
NVAPLKLRWT IAKYAPRFNG FQQQDSQELL AFLLDGLHED LNRVHEKPYV
860 870 880 890 900
ELKDSDGRPD WEVAAEAWDN HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS
910 920 930 940 950
VRFDPFNFLS LPLPMDSYMH LEITVIKLDG TTPVRYGLRL NMDEKYTGLK
960 970 980 990 1000
KQLSDLCGLN SEQILLAEVH GSNIKNFPQD NQKVRLSVSG FLCAFEIPVP
1010 1020 1030 1040 1050
VSPISASSPT QTDFSSSPST NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG
1060 1070 1080 1090 1100
TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH RKMMRTELYF LSSQKNRPSL
1110 1120 1130 1140 1150
FGMPLIVPCT VHTRKKDLYD AVWIQVSRLA SPLPPQEASN HAQDCDDSMG
1160 1170 1180 1190 1200
YQYPFTLRVV QKDGNSCAWC PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD
1210 1220 1230 1240 1250
PTALHLRYQT SQERVVDEHE SVEQSRRAQA EPINLDSCLR AFTSEEELGE
1260 1270 1280 1290 1300
NEMYYCSKCK THCLATKKLD LWRLPPILII HLKRFQFVNG RWIKSQKIVK
1310 1320 1330 1340 1350
FPRESFDPSA FLVPRDPALC QHKPLTPQGD ELSEPRILAR EVKKVDAQSS
1360 1370 1380 1390 1400
AGEEDVLLSK SPSSLSANII SSPKGSPSSS RKSGTSCPSS KNSSPNSSPR
1410 1420 1430 1440 1450
TLGRSKGRLR LPQIGSKNKL SSSKENLDAS KENGAGQICE LADALSRGHV
1460 1470 1480 1490 1500
LGGSQPELVT PQDHEVALAN GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ
1510 1520 1530 1540 1550
REDTRIKPIY NLYAISCHSG ILGGGHYVTY AKNPNCKWYC YNDSSCKELH
1560 1570 1580 1590 1600
PDEIDTDSAY ILFYEQQGID YAQFLPKTDG KKMADTSSMD EDFESDYKKY

CVLQ
Length:1,604
Mass (Da):181,656
Last modified:October 1, 2002 - v1
Checksum:iA621F764B76321E3
GO
Isoform 2 (identifier: Q8NFA0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-390: GWLERESRYGL → TLETDQIYTRN
     391-1604: Missing.

Note: No experimental confirmation available.
Show »
Length:390
Mass (Da):44,703
Checksum:i45F2BC9CFE8CECB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti884H → R in AAK30207 (Ref. 2) Curated1
Sequence conflicti1167C → S in AAK30207 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05153676H → Y. Corresponds to variant dbSNP:rs7208980Ensembl.1
Natural variantiVAR_0515371469A → G. Corresponds to variant dbSNP:rs3207630Ensembl.1
Natural variantiVAR_0515381568G → R. Corresponds to variant dbSNP:rs1053621Ensembl.1
Natural variantiVAR_0515391578T → I. Corresponds to variant dbSNP:rs1053625Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056307380 – 390GWLERESRYGL → TLETDQIYTRN in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_056308391 – 1604Missing in isoform 2. 1 PublicationAdd BLAST1214

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533230 mRNA Translation: AAM97922.1
AF350251 mRNA Translation: AAK30207.1
AC003962 Genomic DNA No translation available.
AC025048 Genomic DNA No translation available.
AC037475 Genomic DNA No translation available.
AC104763 Genomic DNA No translation available.
BC054344 mRNA Translation: AAH54344.1
AF155116 mRNA Translation: AAD42882.1
CCDSiCCDS32697.1 [Q8NFA0-1]
RefSeqiNP_115971.2, NM_032582.3 [Q8NFA0-1]
UniGeneiHs.132868

Genome annotation databases

EnsembliENST00000300896; ENSP00000300896; ENSG00000170832 [Q8NFA0-1]
ENST00000393003; ENSP00000376727; ENSG00000170832 [Q8NFA0-2]
GeneIDi84669
KEGGihsa:84669
UCSCiuc002iyo.2 human [Q8NFA0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiUBP32_HUMAN
AccessioniPrimary (citable) accession number: Q8NFA0
Secondary accession number(s): Q7Z5T3, Q9BX85, Q9Y591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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