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Q8NFA0

- UBP32_HUMAN

UniProt

Q8NFA0 - UBP32_HUMAN

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Protein
Ubiquitin carboxyl-terminal hydrolase 32
Gene
USP32, USP10
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei743 – 7431Nucleophile By similarity
Active sitei1526 – 15261Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi241 – 252121 Reviewed prediction
Add
BLAST
Calcium bindingi277 – 288122 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. ubiquitin-specific protease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. protein deubiquitination Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC19.044.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 32 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 32
Renal carcinoma antigen NY-REN-60
Ubiquitin thioesterase 32
Ubiquitin-specific-processing protease 32
Gene namesi
Name:USP32
Synonyms:USP10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:19143. USP32.

Subcellular locationi

Membrane; Lipid-anchor Reviewed prediction. Golgi apparatus 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134982542.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16011601Ubiquitin carboxyl-terminal hydrolase 32
PRO_0000080663Add
BLAST
Propeptidei1602 – 16043Removed in mature form Reviewed prediction
PRO_0000396658

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1173 – 11731Phosphotyrosine1 Publication
Modified residuei1372 – 13721Phosphoserine2 Publications
Modified residuei1601 – 16011Cysteine methyl ester Reviewed prediction
Lipidationi1601 – 16011S-farnesyl cysteine Reviewed prediction

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ8NFA0.
PaxDbiQ8NFA0.
PRIDEiQ8NFA0.

PTM databases

PhosphoSiteiQ8NFA0.

Expressioni

Gene expression databases

ArrayExpressiQ8NFA0.
BgeeiQ8NFA0.
CleanExiHS_USP10.
HS_USP32.
GenevestigatoriQ8NFA0.

Organism-specific databases

HPAiHPA044365.

Interactioni

Protein-protein interaction databases

BioGridi124189. 26 interactions.
IntActiQ8NFA0. 19 interactions.
MINTiMINT-6779170.
STRINGi9606.ENSP00000300896.

Structurei

3D structure databases

ProteinModelPortaliQ8NFA0.
SMRiQ8NFA0. Positions 15-49, 179-340, 730-915, 1233-1312, 1509-1565.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 12636EF-hand 1
Add
BLAST
Domaini228 – 26336EF-hand 2
Add
BLAST
Domaini264 – 29936EF-hand 3
Add
BLAST
Domaini369 – 585217DUSP
Add
BLAST
Domaini734 – 1567834USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 DUSP domain.
Contains 3 EF-hand domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5210.
HOGENOMiHOG000007511.
HOVERGENiHBG057958.
InParanoidiQ8NFA0.
KOiK11837.
OMAiSDNNNQC.
OrthoDBiEOG7PP55V.
PhylomeDBiQ8NFA0.
TreeFamiTF324190.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.30.2230.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF13202. EF-hand_5. 2 hits.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NFA0-1 [UniParc]FASTAAdd to Basket

« Hide

MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE     50
VLGDGVPPKV AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG KDEEKAKYIF 100
SLFSSESGNY VIREEMERML HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL 150
FLNKDAFTFS RWLLSGGVYV TLTDDSDTPT FYQTLAGVTH LEESDIIDLE 200
KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF DENRDNHIDF 250
KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRVELR DMVVALLEVW 300
KDNRTDDIPE LHMDLSDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN 350
EFLNLLFQVC HIVLGLRPAT PEEEGQIIRG WLERESRYGL QAGHNWFIIS 400
MQWWQQWKEY VKYDANPVVI EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS 450
LSYVNTTEEK FSDNISTASE ASETAGSGFL YSATPGADVC FARQHNTSDN 500
NNQCLLGANG NILLHLNPQK PGAIDNQPLV TQEPVKATSL TLEGGRLKRT 550
PQLIHGRDYE MVPEPVWRAL YHWYGANLAL PRPVIKNSKT DIPELELFPR 600
YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA PLKRVLAYTG CFSRMQTIKE 650
IHEYLSQRLR IKEEDMRLWL YNSENYLTLL DDEDHKLEYL KIQDEQHLVI 700
EVRNKDMSWP EEMSFIANSS KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ 750
CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK GHMAKCYGDL VQELWSGTQK 800
NVAPLKLRWT IAKYAPRFNG FQQQDSQELL AFLLDGLHED LNRVHEKPYV 850
ELKDSDGRPD WEVAAEAWDN HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS 900
VRFDPFNFLS LPLPMDSYMH LEITVIKLDG TTPVRYGLRL NMDEKYTGLK 950
KQLSDLCGLN SEQILLAEVH GSNIKNFPQD NQKVRLSVSG FLCAFEIPVP 1000
VSPISASSPT QTDFSSSPST NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG 1050
TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH RKMMRTELYF LSSQKNRPSL 1100
FGMPLIVPCT VHTRKKDLYD AVWIQVSRLA SPLPPQEASN HAQDCDDSMG 1150
YQYPFTLRVV QKDGNSCAWC PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD 1200
PTALHLRYQT SQERVVDEHE SVEQSRRAQA EPINLDSCLR AFTSEEELGE 1250
NEMYYCSKCK THCLATKKLD LWRLPPILII HLKRFQFVNG RWIKSQKIVK 1300
FPRESFDPSA FLVPRDPALC QHKPLTPQGD ELSEPRILAR EVKKVDAQSS 1350
AGEEDVLLSK SPSSLSANII SSPKGSPSSS RKSGTSCPSS KNSSPNSSPR 1400
TLGRSKGRLR LPQIGSKNKL SSSKENLDAS KENGAGQICE LADALSRGHV 1450
LGGSQPELVT PQDHEVALAN GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ 1500
REDTRIKPIY NLYAISCHSG ILGGGHYVTY AKNPNCKWYC YNDSSCKELH 1550
PDEIDTDSAY ILFYEQQGID YAQFLPKTDG KKMADTSSMD EDFESDYKKY 1600
CVLQ 1604
Length:1,604
Mass (Da):181,656
Last modified:October 1, 2002 - v1
Checksum:iA621F764B76321E3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti76 – 761H → Y.
Corresponds to variant rs7208980 [ dbSNP | Ensembl ].
VAR_051536
Natural varianti1469 – 14691A → G.
Corresponds to variant rs3207630 [ dbSNP | Ensembl ].
VAR_051537
Natural varianti1568 – 15681G → R.
Corresponds to variant rs16944142 [ dbSNP | Ensembl ].
VAR_051538
Natural varianti1578 – 15781T → I.
Corresponds to variant rs16944136 [ dbSNP | Ensembl ].
VAR_051539

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti884 – 8841H → R in AAK30207. 1 Publication
Sequence conflicti1167 – 11671C → S in AAK30207. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF533230 mRNA. Translation: AAM97922.1.
AF350251 mRNA. Translation: AAK30207.1.
AF155116 mRNA. Translation: AAD42882.1.
CCDSiCCDS32697.1.
RefSeqiNP_115971.2. NM_032582.3.
UniGeneiHs.132868.

Genome annotation databases

EnsembliENST00000300896; ENSP00000300896; ENSG00000170832.
GeneIDi84669.
KEGGihsa:84669.
UCSCiuc002iyn.1. human.

Polymorphism databases

DMDMi47606649.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF533230 mRNA. Translation: AAM97922.1 .
AF350251 mRNA. Translation: AAK30207.1 .
AF155116 mRNA. Translation: AAD42882.1 .
CCDSi CCDS32697.1.
RefSeqi NP_115971.2. NM_032582.3.
UniGenei Hs.132868.

3D structure databases

ProteinModelPortali Q8NFA0.
SMRi Q8NFA0. Positions 15-49, 179-340, 730-915, 1233-1312, 1509-1565.
ModBasei Search...

Protein-protein interaction databases

BioGridi 124189. 26 interactions.
IntActi Q8NFA0. 19 interactions.
MINTi MINT-6779170.
STRINGi 9606.ENSP00000300896.

Protein family/group databases

MEROPSi C19.044.

PTM databases

PhosphoSitei Q8NFA0.

Polymorphism databases

DMDMi 47606649.

Proteomic databases

MaxQBi Q8NFA0.
PaxDbi Q8NFA0.
PRIDEi Q8NFA0.

Protocols and materials databases

DNASUi 84669.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000300896 ; ENSP00000300896 ; ENSG00000170832 .
GeneIDi 84669.
KEGGi hsa:84669.
UCSCi uc002iyn.1. human.

Organism-specific databases

CTDi 84669.
GeneCardsi GC17M058254.
HGNCi HGNC:19143. USP32.
HPAi HPA044365.
MIMi 607740. gene.
neXtProti NX_Q8NFA0.
PharmGKBi PA134982542.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5210.
HOGENOMi HOG000007511.
HOVERGENi HBG057958.
InParanoidi Q8NFA0.
KOi K11837.
OMAi SDNNNQC.
OrthoDBi EOG7PP55V.
PhylomeDBi Q8NFA0.
TreeFami TF324190.

Miscellaneous databases

ChiTaRSi USP32. human.
GenomeRNAii 84669.
NextBioi 74669.
PROi Q8NFA0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8NFA0.
Bgeei Q8NFA0.
CleanExi HS_USP10.
HS_USP32.
Genevestigatori Q8NFA0.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
3.30.2230.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF06337. DUSP. 1 hit.
PF13202. EF-hand_5. 2 hits.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Identification of a novel ubiquitin specific protease gene related to testes development from human testes cDNA library."
    Sha J.H.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 331-1604.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 536-1363, IDENTIFICATION AS A RENAL CANCER ANTIGEN.
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Towards complete sets of farnesylated and geranylgeranylated proteins."
    Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F.
    PLoS Comput. Biol. 3:634-648(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-1601.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1173, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. "USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers."
    Akhavantabasi S., Akman H.B., Sapmaz A., Keller J., Petty E.M., Erson A.E.
    Mamm. Genome 21:388-397(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP32_HUMAN
AccessioniPrimary (citable) accession number: Q8NFA0
Secondary accession number(s): Q9BX85, Q9Y591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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