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Q8NFA0 (UBP32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 32

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 32
Renal carcinoma antigen NY-REN-60
Ubiquitin thioesterase 32
Ubiquitin-specific-processing protease 32
Gene names
Name:USP32
Synonyms:USP10
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.8

Subcellular location

Membrane; Lipid-anchor Potential. Golgi apparatus Ref.8.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 DUSP domain.

Contains 3 EF-hand domains.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16011601Ubiquitin carboxyl-terminal hydrolase 32
PRO_0000080663
Propeptide1602 – 16043Removed in mature form Potential
PRO_0000396658

Regions

Domain91 – 12636EF-hand 1
Domain228 – 26336EF-hand 2
Domain264 – 29936EF-hand 3
Domain369 – 585217DUSP
Domain734 – 1567834USP
Calcium binding241 – 252121 Potential
Calcium binding277 – 288122 Potential

Sites

Active site7431Nucleophile By similarity
Active site15261Proton acceptor By similarity

Amino acid modifications

Modified residue11731Phosphotyrosine Ref.6
Modified residue13721Phosphoserine Ref.7 Ref.9
Modified residue16011Cysteine methyl ester Potential
Lipidation16011S-farnesyl cysteine Potential

Natural variations

Natural variant761H → Y.
Corresponds to variant rs7208980 [ dbSNP | Ensembl ].
VAR_051536
Natural variant14691A → G.
Corresponds to variant rs3207630 [ dbSNP | Ensembl ].
VAR_051537
Natural variant15681G → R.
Corresponds to variant rs16944142 [ dbSNP | Ensembl ].
VAR_051538
Natural variant15781T → I.
Corresponds to variant rs16944136 [ dbSNP | Ensembl ].
VAR_051539

Experimental info

Sequence conflict8841H → R in AAK30207. Ref.2
Sequence conflict11671C → S in AAK30207. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8NFA0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A621F764B76321E3

FASTA1,604181,656
        10         20         30         40         50         60 
MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE VLGDGVPPKV 

        70         80         90        100        110        120 
AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG KDEEKAKYIF SLFSSESGNY VIREEMERML 

       130        140        150        160        170        180 
HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL FLNKDAFTFS RWLLSGGVYV TLTDDSDTPT 

       190        200        210        220        230        240 
FYQTLAGVTH LEESDIIDLE KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF 

       250        260        270        280        290        300 
DENRDNHIDF KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRVELR DMVVALLEVW 

       310        320        330        340        350        360 
KDNRTDDIPE LHMDLSDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN EFLNLLFQVC 

       370        380        390        400        410        420 
HIVLGLRPAT PEEEGQIIRG WLERESRYGL QAGHNWFIIS MQWWQQWKEY VKYDANPVVI 

       430        440        450        460        470        480 
EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS LSYVNTTEEK FSDNISTASE ASETAGSGFL 

       490        500        510        520        530        540 
YSATPGADVC FARQHNTSDN NNQCLLGANG NILLHLNPQK PGAIDNQPLV TQEPVKATSL 

       550        560        570        580        590        600 
TLEGGRLKRT PQLIHGRDYE MVPEPVWRAL YHWYGANLAL PRPVIKNSKT DIPELELFPR 

       610        620        630        640        650        660 
YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA PLKRVLAYTG CFSRMQTIKE IHEYLSQRLR 

       670        680        690        700        710        720 
IKEEDMRLWL YNSENYLTLL DDEDHKLEYL KIQDEQHLVI EVRNKDMSWP EEMSFIANSS 

       730        740        750        760        770        780 
KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK 

       790        800        810        820        830        840 
GHMAKCYGDL VQELWSGTQK NVAPLKLRWT IAKYAPRFNG FQQQDSQELL AFLLDGLHED 

       850        860        870        880        890        900 
LNRVHEKPYV ELKDSDGRPD WEVAAEAWDN HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS 

       910        920        930        940        950        960 
VRFDPFNFLS LPLPMDSYMH LEITVIKLDG TTPVRYGLRL NMDEKYTGLK KQLSDLCGLN 

       970        980        990       1000       1010       1020 
SEQILLAEVH GSNIKNFPQD NQKVRLSVSG FLCAFEIPVP VSPISASSPT QTDFSSSPST 

      1030       1040       1050       1060       1070       1080 
NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH 

      1090       1100       1110       1120       1130       1140 
RKMMRTELYF LSSQKNRPSL FGMPLIVPCT VHTRKKDLYD AVWIQVSRLA SPLPPQEASN 

      1150       1160       1170       1180       1190       1200 
HAQDCDDSMG YQYPFTLRVV QKDGNSCAWC PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD 

      1210       1220       1230       1240       1250       1260 
PTALHLRYQT SQERVVDEHE SVEQSRRAQA EPINLDSCLR AFTSEEELGE NEMYYCSKCK 

      1270       1280       1290       1300       1310       1320 
THCLATKKLD LWRLPPILII HLKRFQFVNG RWIKSQKIVK FPRESFDPSA FLVPRDPALC 

      1330       1340       1350       1360       1370       1380 
QHKPLTPQGD ELSEPRILAR EVKKVDAQSS AGEEDVLLSK SPSSLSANII SSPKGSPSSS 

      1390       1400       1410       1420       1430       1440 
RKSGTSCPSS KNSSPNSSPR TLGRSKGRLR LPQIGSKNKL SSSKENLDAS KENGAGQICE 

      1450       1460       1470       1480       1490       1500 
LADALSRGHV LGGSQPELVT PQDHEVALAN GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ 

      1510       1520       1530       1540       1550       1560 
REDTRIKPIY NLYAISCHSG ILGGGHYVTY AKNPNCKWYC YNDSSCKELH PDEIDTDSAY 

      1570       1580       1590       1600 
ILFYEQQGID YAQFLPKTDG KKMADTSSMD EDFESDYKKY CVLQ 

« Hide

References

« Hide 'large scale' references
[1]"The Tre2 (USP6) oncogene is a hominoid-specific gene."
Paulding C.A., Ruvolo M., Haber D.A.
Proc. Natl. Acad. Sci. U.S.A. 100:2507-2511(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Identification of a novel ubiquitin specific protease gene related to testes development from human testes cDNA library."
Sha J.H.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 331-1604.
Tissue: Testis.
[3]"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J.
Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 536-1363, IDENTIFICATION AS A RENAL CANCER ANTIGEN.
[4]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Towards complete sets of farnesylated and geranylgeranylated proteins."
Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F.
PLoS Comput. Biol. 3:634-648(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-1601.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1173, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"USP32 is an active, membrane-bound ubiquitin protease overexpressed in breast cancers."
Akhavantabasi S., Akman H.B., Sapmaz A., Keller J., Petty E.M., Erson A.E.
Mamm. Genome 21:388-397(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF533230 mRNA. Translation: AAM97922.1.
AF350251 mRNA. Translation: AAK30207.1.
AF155116 mRNA. Translation: AAD42882.1.
CCDSCCDS32697.1.
RefSeqNP_115971.2. NM_032582.3.
UniGeneHs.132868.

3D structure databases

ProteinModelPortalQ8NFA0.
SMRQ8NFA0. Positions 15-49, 179-340, 730-915, 1233-1312, 1509-1565.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124189. 26 interactions.
IntActQ8NFA0. 19 interactions.
MINTMINT-6779170.
STRING9606.ENSP00000300896.

Protein family/group databases

MEROPSC19.044.

PTM databases

PhosphoSiteQ8NFA0.

Polymorphism databases

DMDM47606649.

Proteomic databases

MaxQBQ8NFA0.
PaxDbQ8NFA0.
PRIDEQ8NFA0.

Protocols and materials databases

DNASU84669.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300896; ENSP00000300896; ENSG00000170832.
GeneID84669.
KEGGhsa:84669.
UCSCuc002iyn.1. human.

Organism-specific databases

CTD84669.
GeneCardsGC17M058254.
HGNCHGNC:19143. USP32.
HPAHPA044365.
MIM607740. gene.
neXtProtNX_Q8NFA0.
PharmGKBPA134982542.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5210.
HOGENOMHOG000007511.
HOVERGENHBG057958.
InParanoidQ8NFA0.
KOK11837.
OMASDNNNQC.
OrthoDBEOG7PP55V.
PhylomeDBQ8NFA0.
TreeFamTF324190.

Gene expression databases

ArrayExpressQ8NFA0.
BgeeQ8NFA0.
CleanExHS_USP10.
HS_USP32.
GenevestigatorQ8NFA0.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
3.30.2230.10. 2 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF06337. DUSP. 1 hit.
PF13202. EF-hand_5. 2 hits.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00695. DUSP. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF143791. SSF143791. 2 hits.
PROSITEPS51283. DUSP. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP32. human.
GenomeRNAi84669.
NextBio74669.
PROQ8NFA0.
SOURCESearch...

Entry information

Entry nameUBP32_HUMAN
AccessionPrimary (citable) accession number: Q8NFA0
Secondary accession number(s): Q9BX85, Q9Y591
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM