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Protein

Zinc finger protein 397

Gene

ZNF397

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 3 acts as a DNA-dependent transcriptional repressor.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri285 – 307C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 335C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri341 – 363C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri397 – 419C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri425 – 447C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri453 – 475C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri481 – 503C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri509 – 531C2H2-type 9PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 397
Alternative name(s):
Zinc finger and SCAN domain-containing protein 15
Zinc finger protein 47
Gene namesi
Name:ZNF397
Synonyms:ZNF47, ZSCAN15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:18818. ZNF397.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000186812.
PharmGKBiPA38694.

Polymorphism and mutation databases

BioMutaiZNF397.
DMDMi38258943.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475641 – 534Zinc finger protein 397Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphoserineCombined sources1
Cross-linki171Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8NF99.
MaxQBiQ8NF99.
PaxDbiQ8NF99.
PeptideAtlasiQ8NF99.
PRIDEiQ8NF99.

PTM databases

iPTMnetiQ8NF99.
PhosphoSitePlusiQ8NF99.

Expressioni

Tissue specificityi

Expressed strongly in testis, moderately in skeletal muscle, pancreas and prostate, and weakly in heart, placenta, liver, kidney, spleen, thymus and small intestine.1 Publication

Gene expression databases

BgeeiENSG00000186812.
CleanExiHS_ZNF397.
ExpressionAtlasiQ8NF99. baseline and differential.
GenevisibleiQ8NF99. HS.

Organism-specific databases

HPAiHPA026087.

Interactioni

Subunit structurei

Isoforms 1 and 3 can both homo- and hetero-associate. Homo-association of isoform 1 is dependent on the presence of the SCAN domain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LMO4P619683EBI-10213894,EBI-2798728
PRKAA2P546463EBI-10213894,EBI-1383852
ZNF410Q86VK43EBI-10213894,EBI-720304
ZNF446A0A087WUM84EBI-11524467,EBI-11955977
ZNF446Q96AF55EBI-10213894,EBI-740232

Protein-protein interaction databases

BioGridi124033. 36 interactors.
IntActiQ8NF99. 6 interactors.
STRINGi9606.ENSP00000331577.

Structurei

3D structure databases

ProteinModelPortaliQ8NF99.
SMRiQ8NF99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 132SCAN boxPROSITE-ProRule annotationAdd BLAST83

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri285 – 307C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 335C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri341 – 363C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri397 – 419C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri425 – 447C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri453 – 475C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri481 – 503C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri509 – 531C2H2-type 9PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118881.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ8NF99.
KOiK09230.
OMAiWEPCLSP.
OrthoDBiEOG091G02KC.
PhylomeDBiQ8NF99.
TreeFamiTF338304.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 7 hits.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 9 hits.
PS50157. ZINC_FINGER_C2H2_2. 9 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NF99-1) [UniParc]FASTAAdd to basket
Also known as: ZNF397-fu

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR
60 70 80 90 100
QQFRKFCYQE TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS
110 120 130 140 150
ILPEELQIWV QQHNPESGEE AVTLLEDLER EFDDPGQQVP ASPQGPAVPW
160 170 180 190 200
KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC LSPKSDCENS ETATKEGISE
210 220 230 240 250
EKSQGLPQEP SFRGISEHES NLVWKQGSAT GEKLRSPSQG GSFSQVIFTN
260 270 280 290 300
KSLGKRDLYD EAERCLILTT DSIMCQKVPP EERPYRCDVC GHSFKQHSSL
310 320 330 340 350
TQHQRIHTGE KPYKCNQCGK AFSLRSYLII HQRIHSGEKA YECSECGKAF
360 370 380 390 400
NQSSALIRHR KIHTGEKACK CNECGKAFSQ SSYLIIHQRI HTGEKPYECN
410 420 430 440 450
ECGKTFSQSS KLIRHQRIHT GERPYECNEC GKAFRQSSEL ITHQRIHSGE
460 470 480 490 500
KPYECSECGK AFSLSSNLIR HQRIHSGEEP YQCNECGKTF KRSSALVQHQ
510 520 530
RIHSGDEAYI CNECGKAFRH RSVLMRHQRV HTIK
Length:534
Mass (Da):61,139
Last modified:October 31, 2003 - v2
Checksum:iE990609DABABDE2A
GO
Isoform 2 (identifier: Q8NF99-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-275: SEHESNLVWK...LILTTDSIMC → KLSRPPKASS...SKNVNRKYFS
     276-534: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):31,052
Checksum:iB745896D4FDD142E
GO
Isoform 3 (identifier: Q8NF99-3) [UniParc]FASTAAdd to basket
Also known as: Truncated, ZNF397-nf

The sequence of this isoform differs from the canonical sequence as follows:
     186-207: DCENSETATKEGISEEKSQGLP → GEEWEIIWKLLTLRPLFLRMQN
     208-534: Missing.

Show »
Length:207
Mass (Da):24,141
Checksum:iBC397F169EF9962E
GO

Sequence cautioni

Isoform 3 : The sequence AAN65175 differs from that shown. Reason: Frameshift at position 194.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042491186 – 207DCENS…SQGLP → GEEWEIIWKLLTLRPLFLRM QN in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_042492208 – 534Missing in isoform 3. 1 PublicationAdd BLAST327
Alternative sequenceiVSP_006924216 – 275SEHES…DSIMC → KLSRPPKASSAIRWECVSPG SFPGDIIAAEATHSTISCFA INTLPATILPSKNVNRKYFS in isoform 2. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_006925276 – 534Missing in isoform 2. 1 PublicationAdd BLAST259

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533250 mRNA. Translation: AAM95991.2.
AY157641 mRNA. Translation: AAN65175.1. Frameshift.
BC006172 mRNA. Translation: AAH06172.1.
CCDSiCCDS32814.1. [Q8NF99-2]
CCDS45852.1. [Q8NF99-1]
RefSeqiNP_001128650.1. NM_001135178.2. [Q8NF99-1]
NP_115723.1. NM_032347.2. [Q8NF99-2]
XP_011524533.1. XM_011526231.2. [Q8NF99-1]
XP_016881531.1. XM_017026042.1. [Q8NF99-1]
UniGeneiHs.591061.

Genome annotation databases

EnsembliENST00000261333; ENSP00000261333; ENSG00000186812. [Q8NF99-2]
ENST00000330501; ENSP00000331577; ENSG00000186812. [Q8NF99-1]
ENST00000585800; ENSP00000466760; ENSG00000186812. [Q8NF99-3]
ENST00000591206; ENSP00000466317; ENSG00000186812. [Q8NF99-3]
GeneIDi84307.
KEGGihsa:84307.
UCSCiuc002kyi.4. human. [Q8NF99-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533250 mRNA. Translation: AAM95991.2.
AY157641 mRNA. Translation: AAN65175.1. Frameshift.
BC006172 mRNA. Translation: AAH06172.1.
CCDSiCCDS32814.1. [Q8NF99-2]
CCDS45852.1. [Q8NF99-1]
RefSeqiNP_001128650.1. NM_001135178.2. [Q8NF99-1]
NP_115723.1. NM_032347.2. [Q8NF99-2]
XP_011524533.1. XM_011526231.2. [Q8NF99-1]
XP_016881531.1. XM_017026042.1. [Q8NF99-1]
UniGeneiHs.591061.

3D structure databases

ProteinModelPortaliQ8NF99.
SMRiQ8NF99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124033. 36 interactors.
IntActiQ8NF99. 6 interactors.
STRINGi9606.ENSP00000331577.

PTM databases

iPTMnetiQ8NF99.
PhosphoSitePlusiQ8NF99.

Polymorphism and mutation databases

BioMutaiZNF397.
DMDMi38258943.

Proteomic databases

EPDiQ8NF99.
MaxQBiQ8NF99.
PaxDbiQ8NF99.
PeptideAtlasiQ8NF99.
PRIDEiQ8NF99.

Protocols and materials databases

DNASUi84307.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261333; ENSP00000261333; ENSG00000186812. [Q8NF99-2]
ENST00000330501; ENSP00000331577; ENSG00000186812. [Q8NF99-1]
ENST00000585800; ENSP00000466760; ENSG00000186812. [Q8NF99-3]
ENST00000591206; ENSP00000466317; ENSG00000186812. [Q8NF99-3]
GeneIDi84307.
KEGGihsa:84307.
UCSCiuc002kyi.4. human. [Q8NF99-1]

Organism-specific databases

CTDi84307.
GeneCardsiZNF397.
HGNCiHGNC:18818. ZNF397.
HPAiHPA026087.
MIMi609601. gene.
neXtProtiNX_Q8NF99.
OpenTargetsiENSG00000186812.
PharmGKBiPA38694.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118881.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ8NF99.
KOiK09230.
OMAiWEPCLSP.
OrthoDBiEOG091G02KC.
PhylomeDBiQ8NF99.
TreeFamiTF338304.

Miscellaneous databases

ChiTaRSiZNF397. human.
GenomeRNAii84307.
PROiQ8NF99.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186812.
CleanExiHS_ZNF397.
ExpressionAtlasiQ8NF99. baseline and differential.
GenevisibleiQ8NF99. HS.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 7 hits.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 9 hits.
PS50157. ZINC_FINGER_C2H2_2. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN397_HUMAN
AccessioniPrimary (citable) accession number: Q8NF99
Secondary accession number(s): Q9BRM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 31, 2003
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.