Q8NF99 (ZN397_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 397

Alternative name(s):
Zinc finger and SCAN domain-containing protein 15
Zinc finger protein 47

Gene names

Name:	ZNF397
Synonyms:	ZNF47, ZSCAN15

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 3 acts as a DNA-dependent transcriptional repressor. Ref.1
Subunit structure	Isoforms 1 and 3 can both homo- and hetero-associate. Homo-association of isoform 1 is dependent on the presence of the SCAN domain. Ref.1
Subcellular location	Isoform 1: Nucleus. Isoform 3: Nucleus. Cytoplasm Ref.1.
Tissue specificity	Expressed strongly in testis, moderately in skeletal muscle, pancreas and prostate, and weakly in heart, placenta, liver, kidney, spleen, thymus and small intestine. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 9 C2H2-type zinc fingers. Contains 1 SCAN box domain.
Sequence caution	Isoform 3: The sequence AAN65175.1 differs from that shown. Reason: Frameshift at position 194.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral reproduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NF99-1)

Also known as: ZNF397-fu;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NF99-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-275: SEHESNLVWK...LILTTDSIMC → KLSRPPKASS...SKNVNRKYFS
     276-534: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NF99-3)

Also known as: Truncated; ZNF397-nf;

The sequence of this isoform differs from the canonical sequence as follows:
     186-207: DCENSETATKEGISEEKSQGLP → GEEWEIIWKLLTLRPLFLRMQN
     208-534: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Zinc finger protein 397		PRO_0000047564
Regions
	Domain	50 – 132	83	SCAN box
	Zinc finger	285 – 307	23	C2H2-type 1
	Zinc finger	313 – 335	23	C2H2-type 2
	Zinc finger	341 – 363	23	C2H2-type 3
	Zinc finger	369 – 391	23	C2H2-type 4
	Zinc finger	397 – 419	23	C2H2-type 5
	Zinc finger	425 – 447	23	C2H2-type 6
	Zinc finger	453 – 475	23	C2H2-type 7
	Zinc finger	481 – 503	23	C2H2-type 8
	Zinc finger	509 – 531	23	C2H2-type 9
Natural variations
	Alternative sequence	186 – 207	22	DCENS…SQGLP → GEEWEIIWKLLTLRPLFLRM QN in isoform 3.		VSP_042491
	Alternative sequence	208 – 534	327	Missing in isoform 3.		VSP_042492
	Alternative sequence	216 – 275	60	SEHES…DSIMC → KLSRPPKASSAIRWECVSPG SFPGDIIAAEATHSTISCFA INTLPATILPSKNVNRKYFS in isoform 2.		VSP_006924
	Alternative sequence	276 – 534	259	Missing in isoform 2.		VSP_006925

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ZNF397-fu) [UniParc]. Last modified October 31, 2003. Version 2. Checksum: E990609DABABDE2A	FASTA	534	61,139
	10 20 30 40 50 60 MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR QQFRKFCYQE 70 80 90 100 110 120 TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS ILPEELQIWV QQHNPESGEE 130 140 150 160 170 180 AVTLLEDLER EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC 190 200 210 220 230 240 LSPKSDCENS ETATKEGISE EKSQGLPQEP SFRGISEHES NLVWKQGSAT GEKLRSPSQG 250 260 270 280 290 300 GSFSQVIFTN KSLGKRDLYD EAERCLILTT DSIMCQKVPP EERPYRCDVC GHSFKQHSSL 310 320 330 340 350 360 TQHQRIHTGE KPYKCNQCGK AFSLRSYLII HQRIHSGEKA YECSECGKAF NQSSALIRHR 370 380 390 400 410 420 KIHTGEKACK CNECGKAFSQ SSYLIIHQRI HTGEKPYECN ECGKTFSQSS KLIRHQRIHT 430 440 450 460 470 480 GERPYECNEC GKAFRQSSEL ITHQRIHSGE KPYECSECGK AFSLSSNLIR HQRIHSGEEP 490 500 510 520 530 YQCNECGKTF KRSSALVQHQ RIHSGDEAYI CNECGKAFRH RSVLMRHQRV HTIK
	Isoform 2 [UniParc]. Checksum: B745896D4FDD142E	FASTA	275	31,052
	10 20 30 40 50 60 MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR QQFRKFCYQE 70 80 90 100 110 120 TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS ILPEELQIWV QQHNPESGEE 130 140 150 160 170 180 AVTLLEDLER EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC 190 200 210 220 230 240 LSPKSDCENS ETATKEGISE EKSQGLPQEP SFRGIKLSRP PKASSAIRWE CVSPGSFPGD 250 260 270 IIAAEATHST ISCFAINTLP ATILPSKNVN RKYFS
	Isoform 3 (Truncated) (ZNF397-nf) [UniParc]. Checksum: BC397F169EF9962E	FASTA	207	24,141
	10 20 30 40 50 60 MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR QQFRKFCYQE 70 80 90 100 110 120 TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS ILPEELQIWV QQHNPESGEE 130 140 150 160 170 180 AVTLLEDLER EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC 190 200 LSPKSGEEWE IIWKLLTLRP LFLRMQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of two novel human SCAN domain-containing zinc finger genes ZNF396 and ZNF397." Wu Y., Yu L., Bi G., Luo K., Zhou G., Zhao S. Gene 310:193-201(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Hepatoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF533250 mRNA. Translation: AAM95991.2. AY157641 mRNA. Translation: AAN65175.1. Frameshift. BC006172 mRNA. Translation: AAH06172.1.
IPI	IPI00168810. IPI00219791. IPI00385623.
RefSeq	NP_001128650.1. NM_001135178.2. NP_115723.1. NM_032347.2.
UniGene	Hs.591061.
3D structure databases
ProteinModelPortal	Q8NF99.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000331577.
PTM databases
PhosphoSite	Q8NF99.
Polymorphism databases
DMDM	38258943.
Proteomic databases
PaxDb	Q8NF99.
PRIDE	Q8NF99.
Protocols and materials databases
DNASU	84307.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261333; ENSP00000261333; ENSG00000186812. ENST00000330501; ENSP00000331577; ENSG00000186812. ENST00000585800; ENSP00000466760; ENSG00000186812. ENST00000591206; ENSP00000466317; ENSG00000186812.
GeneID	84307.
KEGG	hsa:84307.
UCSC	uc002kyi.3. human. uc002kyj.3. human. uc010dmp.3. human.
Organism-specific databases
CTD	84307.
GeneCards	GC18P032820.
HGNC	HGNC:18818. ZNF397.
HPA	HPA026087.
MIM	609601. gene.
neXtProt	NX_Q8NF99.
PharmGKB	PA38694.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000234619.
HOVERGEN	HBG018163.
InParanoid	Q8NF99.
KO	K09230.
OMA	PSQGGSF.
PhylomeDB	Q8NF99.
Gene expression databases
ArrayExpress	Q8NF99.
Bgee	Q8NF99.
CleanEx	HS_ZNF397.
Genevestigator	Q8NF99.
GermOnline	ENSG00000186812. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 9 hits.
InterPro	IPR008916. Retrov_capsid_C. IPR003309. Tscrpt_reg_SCAN. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF02023. SCAN. 1 hit. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00431. SCAN. 1 hit. SM00355. ZnF_C2H2. 9 hits. [Graphical view]
SUPFAM	SSF47353. Retrov_capsid_C. 1 hit.
PROSITE	PS50804. SCAN_BOX. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 9 hits. PS50157. ZINC_FINGER_C2H2_2. 9 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZNF397. human.
GenomeRNAi	84307.
NextBio	73992.
SOURCE	Search...

Entry information

Entry name

ZN397_HUMAN

Accession

Primary (citable) accession number: Q8NF99
Secondary accession number(s): Q9BRM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	October 31, 2003
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.