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Q8NF99 (ZN397_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 397
Alternative name(s):
Zinc finger and SCAN domain-containing protein 15
Zinc finger protein 47
Gene names
Name:ZNF397
Synonyms:ZNF47, ZSCAN15
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 3 acts as a DNA-dependent transcriptional repressor. Ref.1

Subunit structure

Isoforms 1 and 3 can both homo- and hetero-associate. Homo-association of isoform 1 is dependent on the presence of the SCAN domain. Ref.1

Subcellular location

Isoform 1: Nucleus Ref.1.

Isoform 3: Nucleus. Cytoplasm Ref.1.

Tissue specificity

Expressed strongly in testis, moderately in skeletal muscle, pancreas and prostate, and weakly in heart, placenta, liver, kidney, spleen, thymus and small intestine. Ref.1

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 9 C2H2-type zinc fingers.

Contains 1 SCAN box domain.

Sequence caution

Isoform 3: The sequence AAN65175.1 differs from that shown. Reason: Frameshift at position 194.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NF99-1)

Also known as: ZNF397-fu;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NF99-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-275: SEHESNLVWK...LILTTDSIMC → KLSRPPKASS...SKNVNRKYFS
     276-534: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8NF99-3)

Also known as: Truncated; ZNF397-nf;

The sequence of this isoform differs from the canonical sequence as follows:
     186-207: DCENSETATKEGISEEKSQGLP → GEEWEIIWKLLTLRPLFLRMQN
     208-534: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Zinc finger protein 397
PRO_0000047564

Regions

Domain50 – 13283SCAN box
Zinc finger285 – 30723C2H2-type 1
Zinc finger313 – 33523C2H2-type 2
Zinc finger341 – 36323C2H2-type 3
Zinc finger369 – 39123C2H2-type 4
Zinc finger397 – 41923C2H2-type 5
Zinc finger425 – 44723C2H2-type 6
Zinc finger453 – 47523C2H2-type 7
Zinc finger481 – 50323C2H2-type 8
Zinc finger509 – 53123C2H2-type 9

Natural variations

Alternative sequence186 – 20722DCENS…SQGLP → GEEWEIIWKLLTLRPLFLRM QN in isoform 3.
VSP_042491
Alternative sequence208 – 534327Missing in isoform 3.
VSP_042492
Alternative sequence216 – 27560SEHES…DSIMC → KLSRPPKASSAIRWECVSPG SFPGDIIAAEATHSTISCFA INTLPATILPSKNVNRKYFS in isoform 2.
VSP_006924
Alternative sequence276 – 534259Missing in isoform 2.
VSP_006925

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ZNF397-fu) [UniParc].

Last modified October 31, 2003. Version 2.
Checksum: E990609DABABDE2A

FASTA53461,139
        10         20         30         40         50         60 
MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR QQFRKFCYQE 

        70         80         90        100        110        120 
TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS ILPEELQIWV QQHNPESGEE 

       130        140        150        160        170        180 
AVTLLEDLER EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC 

       190        200        210        220        230        240 
LSPKSDCENS ETATKEGISE EKSQGLPQEP SFRGISEHES NLVWKQGSAT GEKLRSPSQG 

       250        260        270        280        290        300 
GSFSQVIFTN KSLGKRDLYD EAERCLILTT DSIMCQKVPP EERPYRCDVC GHSFKQHSSL 

       310        320        330        340        350        360 
TQHQRIHTGE KPYKCNQCGK AFSLRSYLII HQRIHSGEKA YECSECGKAF NQSSALIRHR 

       370        380        390        400        410        420 
KIHTGEKACK CNECGKAFSQ SSYLIIHQRI HTGEKPYECN ECGKTFSQSS KLIRHQRIHT 

       430        440        450        460        470        480 
GERPYECNEC GKAFRQSSEL ITHQRIHSGE KPYECSECGK AFSLSSNLIR HQRIHSGEEP 

       490        500        510        520        530 
YQCNECGKTF KRSSALVQHQ RIHSGDEAYI CNECGKAFRH RSVLMRHQRV HTIK 

« Hide

Isoform 2 [UniParc].

Checksum: B745896D4FDD142E
Show »

FASTA27531,052
Isoform 3 (Truncated) (ZNF397-nf) [UniParc].

Checksum: BC397F169EF9962E
Show »

FASTA20724,141

References

« Hide 'large scale' references
[1]"Identification and characterization of two novel human SCAN domain-containing zinc finger genes ZNF396 and ZNF397."
Wu Y., Yu L., Bi G., Luo K., Zhou G., Zhao S.
Gene 310:193-201(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Hepatoma.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Uterus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF533250 mRNA. Translation: AAM95991.2.
AY157641 mRNA. Translation: AAN65175.1. Frameshift.
BC006172 mRNA. Translation: AAH06172.1.
CCDSCCDS32814.1. [Q8NF99-2]
CCDS45852.1. [Q8NF99-1]
RefSeqNP_001128650.1. NM_001135178.2. [Q8NF99-1]
NP_115723.1. NM_032347.2. [Q8NF99-2]
UniGeneHs.591061.

3D structure databases

ProteinModelPortalQ8NF99.
SMRQ8NF99. Positions 47-134, 258-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124033. 3 interactions.
STRING9606.ENSP00000331577.

PTM databases

PhosphoSiteQ8NF99.

Polymorphism databases

DMDM38258943.

Proteomic databases

MaxQBQ8NF99.
PaxDbQ8NF99.
PRIDEQ8NF99.

Protocols and materials databases

DNASU84307.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261333; ENSP00000261333; ENSG00000186812. [Q8NF99-2]
ENST00000330501; ENSP00000331577; ENSG00000186812. [Q8NF99-1]
ENST00000585800; ENSP00000466760; ENSG00000186812. [Q8NF99-3]
ENST00000591206; ENSP00000466317; ENSG00000186812. [Q8NF99-3]
GeneID84307.
KEGGhsa:84307.
UCSCuc002kyi.3. human. [Q8NF99-3]
uc002kyj.3. human. [Q8NF99-2]
uc010dmp.3. human. [Q8NF99-1]

Organism-specific databases

CTD84307.
GeneCardsGC18P032820.
HGNCHGNC:18818. ZNF397.
HPAHPA026087.
MIM609601. gene.
neXtProtNX_Q8NF99.
PharmGKBPA38694.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000234619.
HOVERGENHBG018163.
InParanoidQ8NF99.
KOK09230.
OMAIPSIEIC.
PhylomeDBQ8NF99.
TreeFamTF338304.

Gene expression databases

ArrayExpressQ8NF99.
BgeeQ8NF99.
CleanExHS_ZNF397.
GenevestigatorQ8NF99.

Family and domain databases

Gene3D3.30.160.60. 9 hits.
InterProIPR008916. Retrov_capsid_C.
IPR003309. Tscrpt_reg_SCAN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMSSF47353. SSF47353. 1 hit.
PROSITEPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 9 hits.
PS50157. ZINC_FINGER_C2H2_2. 9 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF397. human.
GenomeRNAi84307.
NextBio73992.
PROQ8NF99.
SOURCESearch...

Entry information

Entry nameZN397_HUMAN
AccessionPrimary (citable) accession number: Q8NF99
Secondary accession number(s): Q9BRM2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 31, 2003
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM