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Protein

Serine protease 33

Gene

PRSS33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease that has amidolytic activity, cleaving its substrates before Arg residues.1 Publication

Kineticsi

  1. KM=6.9 mM for H-DL-Val-Leu-Arg-pNA1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771Charge relay systemBy similarity
    Active sitei126 – 1261Charge relay systemBy similarity
    Active sitei231 – 2311Charge relay systemBy similarity

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.075.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine protease 33 (EC:3.4.21.-)
    Alternative name(s):
    Serine protease EOS
    Gene namesi
    Name:PRSS33
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:30405. PRSS33.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134987813.

    Polymorphism and mutation databases

    BioMutaiPRSS33.
    DMDMi212286047.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence analysisAdd
    BLAST
    Chaini23 – 280258Serine protease 33PRO_0000299316Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi62 ↔ 78PROSITE-ProRule annotation
    Disulfide bondi160 ↔ 237PROSITE-ProRule annotation
    Disulfide bondi193 ↔ 216PROSITE-ProRule annotation
    Disulfide bondi227 ↔ 255PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    EPDiQ8NF86.
    PaxDbiQ8NF86.
    PeptideAtlasiQ8NF86.
    PRIDEiQ8NF86.

    PTM databases

    iPTMnetiQ8NF86.
    PhosphoSiteiQ8NF86.

    Expressioni

    Tissue specificityi

    Predominantly expressed in macrophages. Present in the spleen, small and large intestine, lung and brain (at protein level). Highly expressed in peripheral leukocytes, ovary, retina, spleen and stomach. Moderately expressed in thymus, uterus and platelets, as well as some brain tissues, such as thalamus and fetal brain.1 Publication

    Inductioni

    Up-regulated by phorbol myristate acetate (PMA).1 Publication

    Gene expression databases

    BgeeiQ8NF86.
    CleanExiHS_PRSS33.
    ExpressionAtlasiQ8NF86. baseline and differential.
    GenevisibleiQ8NF86. HS.

    Organism-specific databases

    HPAiHPA058082.

    Interactioni

    Protein-protein interaction databases

    BioGridi129278. 6 interactions.
    STRINGi9606.ENSP00000293851.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NF86.
    SMRiQ8NF86. Positions 37-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 279243Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG3627. Eukaryota.
    COG5640. LUCA.
    GeneTreeiENSGT00760000118810.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ8NF86.
    KOiK09629.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ8NF86.
    TreeFamiTF351676.

    Family and domain databases

    InterProiIPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8NF86-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRGVSCLQVL LLLVLGAAGT QGRKSAACGQ PRMSSRIVGG RDGRDGEWPW
    60 70 80 90 100
    QASIQHRGAH VCGGSLIAPQ WVLTAAHCFP RRALPAEYRV RLGALRLGST
    110 120 130 140 150
    SPRTLSVPVR RVLLPPDYSE DGARGDLALL QLRRPVPLSA RVQPVCLPVP
    160 170 180 190 200
    GARPPPGTPC RVTGWGSLRP GVPLPEWRPL QGVRVPLLDS RTCDGLYHVG
    210 220 230 240 250
    ADVPQAERIV LPGSLCAGYP QGHKDACQGD SGGPLTCLQS GSWVLVGVVS
    260 270 280
    WGKGCALPNR PGVYTSVATY SPWIQARVSF
    Length:280
    Mass (Da):29,787
    Last modified:July 22, 2008 - v3
    Checksum:iFCE00004DB1CFBF1
    GO

    Sequence cautioni

    The sequence AAN04055.1 differs from that shown. Reason: Frameshift at position 279. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571R → P in AAN04055 (PubMed:12795636).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF536382 mRNA. Translation: AAN04055.1. Frameshift.
    AC092117 Genomic DNA. No translation available.
    CH471112 Genomic DNA. Translation: EAW85469.1.
    BC036846 mRNA. Translation: AAH36846.2.
    BC062334 mRNA. Translation: AAH62334.1.
    CCDSiCCDS42110.1.
    RefSeqiNP_690851.2. NM_152891.2.
    XP_011520753.1. XM_011522451.1.
    XP_011520754.1. XM_011522452.1.
    UniGeneiHs.280658.

    Genome annotation databases

    EnsembliENST00000293851; ENSP00000293851; ENSG00000103355.
    ENST00000570702; ENSP00000458369; ENSG00000103355.
    GeneIDi260429.
    KEGGihsa:260429.
    UCSCiuc002cro.1. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF536382 mRNA. Translation: AAN04055.1. Frameshift.
    AC092117 Genomic DNA. No translation available.
    CH471112 Genomic DNA. Translation: EAW85469.1.
    BC036846 mRNA. Translation: AAH36846.2.
    BC062334 mRNA. Translation: AAH62334.1.
    CCDSiCCDS42110.1.
    RefSeqiNP_690851.2. NM_152891.2.
    XP_011520753.1. XM_011522451.1.
    XP_011520754.1. XM_011522452.1.
    UniGeneiHs.280658.

    3D structure databases

    ProteinModelPortaliQ8NF86.
    SMRiQ8NF86. Positions 37-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi129278. 6 interactions.
    STRINGi9606.ENSP00000293851.

    Protein family/group databases

    MEROPSiS01.075.

    PTM databases

    iPTMnetiQ8NF86.
    PhosphoSiteiQ8NF86.

    Polymorphism and mutation databases

    BioMutaiPRSS33.
    DMDMi212286047.

    Proteomic databases

    EPDiQ8NF86.
    PaxDbiQ8NF86.
    PeptideAtlasiQ8NF86.
    PRIDEiQ8NF86.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000293851; ENSP00000293851; ENSG00000103355.
    ENST00000570702; ENSP00000458369; ENSG00000103355.
    GeneIDi260429.
    KEGGihsa:260429.
    UCSCiuc002cro.1. human.

    Organism-specific databases

    CTDi260429.
    GeneCardsiPRSS33.
    HGNCiHGNC:30405. PRSS33.
    HPAiHPA058082.
    MIMi613797. gene.
    neXtProtiNX_Q8NF86.
    PharmGKBiPA134987813.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3627. Eukaryota.
    COG5640. LUCA.
    GeneTreeiENSGT00760000118810.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ8NF86.
    KOiK09629.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ8NF86.
    TreeFamiTF351676.

    Miscellaneous databases

    GenomeRNAii260429.
    PROiQ8NF86.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ8NF86.
    CleanExiHS_PRSS33.
    ExpressionAtlasiQ8NF86. baseline and differential.
    GenevisibleiQ8NF86. HS.

    Family and domain databases

    InterProiIPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "A novel serine protease predominately expressed in macrophages."
      Chen C., Darrow A.L., Qi J.-S., D'Andrea M.R., Andrade-Gordon P.
      Biochem. J. 374:97-107(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    2. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Skin.

    Entry informationi

    Entry nameiPRS33_HUMAN
    AccessioniPrimary (citable) accession number: Q8NF86
    Secondary accession number(s): A6NNQ3, Q8N171
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: July 22, 2008
    Last modified: July 6, 2016
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.