Reviewed, UniProtKB/Swiss-Prot Q8NF64 (ZMIZ2_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger MIZ domain-containing protein 2
Alternative name(s):
    PIAS-like protein Zimp7

Gene names

Name:	ZMIZ2
Synonyms:	KIAA1886, ZIMP7
ORF Names:	HRIHFB2007

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Increases ligand-dependent transcriptional activity of AR and other nuclear hormone receptors. Ref.1
Subunit structure	Interacts with AR, SMARCA4/BRG1 and SMARCE1/BAF57. Interaction with either SMARCA4 and SMARCE1 enhances AR-mediated transcription. Ref.1
Subcellular location	Nucleus. Note: Detected at replication foci throughout S phase. Ref.1 Ref.7
Tissue specificity	Expressed most abundantly in testis with lower levels in heart, brain, pancreas, prostate and ovary. Ref.1
Domain	The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein.
Sequence similarities	Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Gene Ontology (GO)
Biological process	positive regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear replication fork Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ligand-dependent nuclear receptor transcription coactivator activity Ref.1 Inferred from direct assay. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NF64-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NF64-2)

The sequence of this isoform differs from the canonical sequence as follows:
     334-359: Missing.

Isoform 3 (identifier: Q8NF64-3)

The sequence of this isoform differs from the canonical sequence as follows:
     56-87: Missing.
     334-359: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Zinc finger MIZ domain-containing protein 2		PRO_0000218989
Regions
	Zinc finger	585 – 662	78	SP-RING-type
	Region	435 – 506	72	Interaction with AR
	Compositional bias	47 – 132	86	Gly-rich
	Compositional bias	147 – 170	24	Ala-rich
	Compositional bias	201 – 403	203	Pro-rich
	Compositional bias	725 – 906	182	Pro-rich
Natural variations
	Alternative sequence	56 – 87	32	Missing in isoform 3.		VSP_024918
	Alternative sequence	334 – 359	26	Missing in isoform 2 and isoform 3.		VSP_012190
	Natural variant	408	1	L → F: dbSNP rs3735478. Ref.2		VAR_050536
Experimental info
	Sequence conflict	127	1	Missing in AAR85526. Ref.1
	Sequence conflict	462	1	R → RR in CAH56269. Ref.6
	Sequence conflict	568	1	Missing Ref.1
	Sequence conflict	765	1	T → M Ref.1
	Sequence conflict	765	1	T → M Ref.4
	Sequence conflict	765	1	T → M in CAB66507. Ref.6
	Sequence conflict	782	1	S → C Ref.7
	Sequence conflict	799	1	A → V Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: C9CE99D0B0798B9F	FASTA	920	96,537
	10 20 30 40 50 60 MNSMNPMKPA LPPAPHGDGS FAYESVPWQQ SATQPAGSLS VVTTVWGVGN ATQSQVLGNP 70 80 90 100 110 120 MGPAGSPSGS SMMPGVAGGS SALTSPQCLG QQAFAEGGAN KGYVQQGVYS RGGYPGAPGF 130 140 150 160 170 180 TTGYAGGPGG LGLPSHAARP STDFTQAAAA AAVAAAAATA TATATATVAA LQEKQSQELS 190 200 210 220 230 240 QYGAMGAGQS FNSQFLQHGG PRGPSVPAGM NPTGIGGVMG PSGLSPLAMN PTRAAGMTPL 250 260 270 280 290 300 YAGQRLPQHG YPGPPQAQPL PRQGVKRTYS EVYPGQQYLQ GGQYAPSTAQ FAPSPGQPPA 310 320 330 340 350 360 PSPSYPGHRL PLQQGMTQSL SVPGPTGLHY KPTEQFNGQG ASFNGGSVSY SQPGLSGPTR 370 380 390 400 410 420 SIPGYPSSPL PGNPTPPMTP SSSVPYMSPN QEVKSPFLPD LKPNLNSLHS SPSGSGPCDE 430 440 450 460 470 480 LRLTFPVRDG VVLEPFRLQH NLAVSNHVFQ LRDSVYKTLI MRPDLELQFK CYHHEDRQMN 490 500 510 520 530 540 TNWPASVQVS VNATPLTIER GDNKTSHKPL YLKHVCQPGR NTIQITVTAC CCSHLFVLQL 550 560 570 580 590 600 VHRPSVRSVL QGLLKKRLLP AEHCITKIKR NFSSGTIPGT PGPNGEDGVE QTAIKVSLKC 610 620 630 640 650 660 PITFRRIQLP ARGHDCRHIQ CFDLESYLQL NCERGTWRCP VCNKTALLEG LEVDQYMLGI 670 680 690 700 710 720 LIYIQNSDYE EITIDPTCSW KPVPVKPDMH IKEEPDGPAL KRCRTVSPAH VLMPSVMEMI 730 740 750 760 770 780 AALGPGAAPF APLQPPSVPA PSDYPGQGSS FLGPGTFPES FPPTTPSTPT LAEFTPGPPP 790 800 810 820 830 840 ISYQSDIPSS LLTSEKSTAC LPSQMAPAGH LDPTHNPGTP GLHTSNLGAP PGPQLHHSNP 850 860 870 880 890 900 PPASRQSLGQ ASLGPTGELA FSPATGVMGP PSMSGAGEAP EPALDLLPEL TNPDELLSYL 910 920 GPPDLPTNNN DDLLSLFENN
	Isoform 2. Checksum: 3E9DFD0FD14F5CAE	FASTA	894	93,981
	10 20 30 40 50 60 MNSMNPMKPA LPPAPHGDGS FAYESVPWQQ SATQPAGSLS VVTTVWGVGN ATQSQVLGNP 70 80 90 100 110 120 MGPAGSPSGS SMMPGVAGGS SALTSPQCLG QQAFAEGGAN KGYVQQGVYS RGGYPGAPGF 130 140 150 160 170 180 TTGYAGGPGG LGLPSHAARP STDFTQAAAA AAVAAAAATA TATATATVAA LQEKQSQELS 190 200 210 220 230 240 QYGAMGAGQS FNSQFLQHGG PRGPSVPAGM NPTGIGGVMG PSGLSPLAMN PTRAAGMTPL 250 260 270 280 290 300 YAGQRLPQHG YPGPPQAQPL PRQGVKRTYS EVYPGQQYLQ GGQYAPSTAQ FAPSPGQPPA 310 320 330 340 350 360 PSPSYPGHRL PLQQGMTQSL SVPGPTGLHY KPTRSIPGYP SSPLPGNPTP PMTPSSSVPY 370 380 390 400 410 420 MSPNQEVKSP FLPDLKPNLN SLHSSPSGSG PCDELRLTFP VRDGVVLEPF RLQHNLAVSN 430 440 450 460 470 480 HVFQLRDSVY KTLIMRPDLE LQFKCYHHED RQMNTNWPAS VQVSVNATPL TIERGDNKTS 490 500 510 520 530 540 HKPLYLKHVC QPGRNTIQIT VTACCCSHLF VLQLVHRPSV RSVLQGLLKK RLLPAEHCIT 550 560 570 580 590 600 KIKRNFSSGT IPGTPGPNGE DGVEQTAIKV SLKCPITFRR IQLPARGHDC RHIQCFDLES 610 620 630 640 650 660 YLQLNCERGT WRCPVCNKTA LLEGLEVDQY MLGILIYIQN SDYEEITIDP TCSWKPVPVK 670 680 690 700 710 720 PDMHIKEEPD GPALKRCRTV SPAHVLMPSV MEMIAALGPG AAPFAPLQPP SVPAPSDYPG 730 740 750 760 770 780 QGSSFLGPGT FPESFPPTTP STPTLAEFTP GPPPISYQSD IPSSLLTSEK STACLPSQMA 790 800 810 820 830 840 PAGHLDPTHN PGTPGLHTSN LGAPPGPQLH HSNPPPASRQ SLGQASLGPT GELAFSPATG 850 860 870 880 890 VMGPPSMSGA GEAPEPALDL LPELTNPDEL LSYLGPPDLP TNNNDDLLSL FENN
	Isoform 3. Checksum: 274013C6083F6D2F	FASTA	862	91,112
	10 20 30 40 50 60 MNSMNPMKPA LPPAPHGDGS FAYESVPWQQ SATQPAGSLS VVTTVWGVGN ATQSQCLGQQ 70 80 90 100 110 120 AFAEGGANKG YVQQGVYSRG GYPGAPGFTT GYAGGPGGLG LPSHAARPST DFTQAAAAAA 130 140 150 160 170 180 VAAAAATATA TATATVAALQ EKQSQELSQY GAMGAGQSFN SQFLQHGGPR GPSVPAGMNP 190 200 210 220 230 240 TGIGGVMGPS GLSPLAMNPT RAAGMTPLYA GQRLPQHGYP GPPQAQPLPR QGVKRTYSEV 250 260 270 280 290 300 YPGQQYLQGG QYAPSTAQFA PSPGQPPAPS PSYPGHRLPL QQGMTQSLSV PGPTGLHYKP 310 320 330 340 350 360 TRSIPGYPSS PLPGNPTPPM TPSSSVPYMS PNQEVKSPFL PDLKPNLNSL HSSPSGSGPC 370 380 390 400 410 420 DELRLTFPVR DGVVLEPFRL QHNLAVSNHV FQLRDSVYKT LIMRPDLELQ FKCYHHEDRQ 430 440 450 460 470 480 MNTNWPASVQ VSVNATPLTI ERGDNKTSHK PLYLKHVCQP GRNTIQITVT ACCCSHLFVL 490 500 510 520 530 540 QLVHRPSVRS VLQGLLKKRL LPAEHCITKI KRNFSSGTIP GTPGPNGEDG VEQTAIKVSL 550 560 570 580 590 600 KCPITFRRIQ LPARGHDCRH IQCFDLESYL QLNCERGTWR CPVCNKTALL EGLEVDQYML 610 620 630 640 650 660 GILIYIQNSD YEEITIDPTC SWKPVPVKPD MHIKEEPDGP ALKRCRTVSP AHVLMPSVME 670 680 690 700 710 720 MIAALGPGAA PFAPLQPPSV PAPSDYPGQG SSFLGPGTFP ESFPPTTPST PTLAEFTPGP 730 740 750 760 770 780 PPISYQSDIP SSLLTSEKST ACLPSQMAPA GHLDPTHNPG TPGLHTSNLG APPGPQLHHS 790 800 810 820 830 840 NPPPASRQSL GQASLGPTGE LAFSPATGVM GPPSMSGAGE APEPALDLLP ELTNPDELLS 850 860 YLGPPDLPTN NNDDLLSLFE NN

References

[1]	"hZimp7, a novel PIAS-like protein, enhances androgen receptor-mediated transcription and interacts with SWI/SNF-like BAF complexes." Huang C.-Y., Beliakoff J., Li X., Lee J., Li X., Sharma M., Lim B., Sun Z. Mol. Endocrinol. 19:2915-2929(2005) [PubMed: 16051670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH AR; SMARCA4 AND SMARCE1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PHE-408. Tissue: Spleen.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 316-920 (ISOFORM 1). Tissue: Kidney and Uterus.
[5]	"Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:179-187(2001) [PubMed: 11572484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 240-920 (ISOFORM 2). Tissue: Brain.
[6]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 332-920. Tissue: Amygdala and Fetal brain.
[7]	"Selection system for genes encoding nuclear-targeted proteins." Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A. Nat. Biotechnol. 16:1338-1342(1998) [PubMed: 9853615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 668-920, SUBCELLULAR LOCATION. Tissue: Fetal brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY426594 mRNA. Translation: AAR85526.1. AK090415 mRNA. Translation: BAC03396.1. Different initiation. AC004859 Genomic DNA. No translation available. AC013436 Genomic DNA. Translation: AAP22368.1. Sequence problems. BC021924 mRNA. Translation: AAH21924.1. Different initiation. BC110435 mRNA. Translation: AAI10436.1. AB067473 mRNA. Translation: BAB67779.1. AL831933 mRNA. Translation: CAH56269.1. AL136572 mRNA. Translation: CAB66507.1. Different initiation. AB015330 mRNA. Translation: BAA34789.1.
IPI	IPI00289799. IPI00302607. IPI00845315.
RefSeq	NP_113637.3. NP_777589.2.
UniGene	Hs.600681 Hs.77978
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NF64. 2 interactions.
PTM databases
PhosphoSite	Q8NF64.
Proteomic databases
PRIDE	Q8NF64.
Genome annotation databases
Ensembl	ENSG00000122515. Homo sapiens. [Contig view]
GeneID	83637.
KEGG	hsa:83637.
Organism-specific databases
GeneCards	GC07P044756.
HGNC	HGNC:22229. ZMIZ2.
MIM	611196. gene.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8NF64.
OMA	Q8NF64. CDELRLT.
Enzyme and pathway databases
Pathway_Interaction_DB	ar_tf_pathway. Regulation of Androgen receptor activity.
Gene expression databases
ArrayExpress	Q8NF64.
Bgee	Q8NF64.
CleanEx	HS_ZMIZ2.
GermOnline	ENSG00000122515. Homo sapiens.
Family and domain databases
InterPro	IPR004181. Znf_MIZ. [Graphical view]
Pfam	PF02891. zf-MIZ. 1 hit. [Graphical view]
PROSITE	PS51044. ZF_SP_RING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	72573.
SOURCE	Search...

Entry information

Entry name

ZMIZ2_HUMAN

Accession

Primary (citable) accession number: Q8NF64
Secondary accession number(s): O94790 , Q0VGB4, Q659A8, Q6JKL5, Q8WTX8, Q96Q01, Q9BQH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)