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Q8NEZ5

- FBX22_HUMAN

UniProt

Q8NEZ5 - FBX22_HUMAN

Protein

F-box only protein 22

Gene

FBXO22

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function.1 Publication

    GO - Molecular functioni

    1. ubiquitin-protein transferase activity Source: ProtInc

    GO - Biological processi

    1. cellular protein modification process Source: ProtInc
    2. cellular response to starvation Source: Ensembl
    3. nucleocytoplasmic transport Source: Ensembl
    4. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
    5. positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: Ensembl
    6. protein polyubiquitination Source: Ensembl
    7. regulation of skeletal muscle fiber development Source: Ensembl
    8. ubiquitin-dependent protein catabolic process Source: ProtInc

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box only protein 22
    Alternative name(s):
    F-box protein FBX22p44
    Gene namesi
    Name:FBXO22
    Synonyms:FBX22
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:13593. FBXO22.

    Subcellular locationi

    CytoplasmmyofibrilsarcomereZ line By similarity

    GO - Cellular componenti

    1. nucleus Source: Ensembl
    2. Z disc Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28035.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403F-box only protein 22PRO_0000119906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine3 Publications
    Modified residuei194 – 1941N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8NEZ5.
    PaxDbiQ8NEZ5.
    PRIDEiQ8NEZ5.

    PTM databases

    PhosphoSiteiQ8NEZ5.

    Expressioni

    Tissue specificityi

    Predominantly expressed in liver, also enriched in cardiac muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ8NEZ5.
    BgeeiQ8NEZ5.
    CleanExiHS_FBXO22.
    GenevestigatoriQ8NEZ5.

    Organism-specific databases

    HPAiHPA003165.
    HPA050178.

    Interactioni

    Subunit structurei

    Directly interacts with SKP1 and CUL1.1 Publication

    Protein-protein interaction databases

    BioGridi117649. 7 interactions.
    IntActiQ8NEZ5. 2 interactions.
    STRINGi9606.ENSP00000307833.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NEZ5.
    SMRiQ8NEZ5. Positions 24-58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 6747F-boxAdd
    BLAST

    Sequence similaritiesi

    Contains 1 F-box domain.Curated

    Phylogenomic databases

    eggNOGiNOG26261.
    HOGENOMiHOG000285967.
    HOVERGENiHBG051570.
    InParanoidiQ8NEZ5.
    KOiK10302.
    OMAiCCKVGAS.
    OrthoDBiEOG72G17J.
    PhylomeDBiQ8NEZ5.
    TreeFamiTF328809.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    IPR019494. FIST_C_domain.
    [Graphical view]
    PfamiPF00646. F-box. 1 hit.
    PF10442. FIST_C. 1 hit.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF81383. SSF81383. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NEZ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPVGCCGEC RGSSVDPRST FVLSNLAEVV ERVLTFLPAK ALLRVACVCR    50
    LWRECVRRVL RTHRSVTWIS AGLAEAGHLE GHCLVRVVAE ELENVRILPH 100
    TVLYMADSET FISLEECRGH KRARKRTSME TALALEKLFP KQCQVLGIVT 150
    PGIVVTPMGS GSNRPQEIEI GESGFALLFP QIEGIKIQPF HFIKDPKNLT 200
    LERHQLTEVG LLDNPELRVV LVFGYNCCKV GASNYLQQVV STFSDMNIIL 250
    AGGQVDNLSS LTSEKNPLDI DASGVVGLSF SGHRIQSATV LLNEDVSDEK 300
    TAEAAMQRLK AANIPEHNTI GFMFACVGRG FQYYRAKGNV EADAFRKFFP 350
    SVPLFGFFGN GEIGCDRIVT GNFILRKCNE VKDDDLFHSY TTIMALIHLG 400
    SSK 403
    Length:403
    Mass (Da):44,508
    Last modified:October 1, 2002 - v1
    Checksum:iD96712BAA1149D8D
    GO
    Isoform 2 (identifier: Q8NEZ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         47-403: Missing.

    Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:46
    Mass (Da):4,922
    Checksum:i1D49258F07775FA2
    GO
    Isoform 3 (identifier: Q8NEZ5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         266-276: NPLDIDASGVV → YVLCASDFVCE
         277-403: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:276
    Mass (Da):30,589
    Checksum:i0E9B0CE3E208358B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 118VGCCGECR → AGACGGP in AAF04523. (PubMed:10531035)Curated
    Sequence conflicti349 – 3491F → L in AAH32540. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei47 – 403357Missing in isoform 2. 1 PublicationVSP_041821Add
    BLAST
    Alternative sequencei266 – 27611NPLDIDASGVV → YVLCASDFVCE in isoform 3. 1 PublicationVSP_013058Add
    BLAST
    Alternative sequencei277 – 403127Missing in isoform 3. 1 PublicationVSP_013059Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY005144 mRNA. Translation: AAF89095.1.
    AK024048 mRNA. Translation: BAB14798.1.
    AC027104 Genomic DNA. No translation available.
    BC032540 mRNA. Translation: AAH32540.1.
    BC020204 mRNA. Translation: AAH20204.2.
    BC039024 mRNA. Translation: AAH39024.1. Sequence problems.
    BC041691 mRNA. Translation: AAH41691.1.
    AF174602 Genomic DNA. Translation: AAF04523.1.
    CCDSiCCDS10287.1. [Q8NEZ5-1]
    CCDS45310.1. [Q8NEZ5-3]
    RefSeqiNP_036302.1. NM_012170.3. [Q8NEZ5-3]
    NP_671717.1. NM_147188.2. [Q8NEZ5-1]
    UniGeneiHs.591115.

    Genome annotation databases

    EnsembliENST00000308275; ENSP00000307833; ENSG00000167196. [Q8NEZ5-1]
    ENST00000453211; ENSP00000396442; ENSG00000167196. [Q8NEZ5-3]
    ENST00000569022; ENSP00000457531; ENSG00000167196. [Q8NEZ5-2]
    GeneIDi26263.
    KEGGihsa:26263.
    UCSCiuc002bbj.2. human. [Q8NEZ5-3]
    uc002bbk.3. human. [Q8NEZ5-1]

    Polymorphism databases

    DMDMi30580428.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY005144 mRNA. Translation: AAF89095.1 .
    AK024048 mRNA. Translation: BAB14798.1 .
    AC027104 Genomic DNA. No translation available.
    BC032540 mRNA. Translation: AAH32540.1 .
    BC020204 mRNA. Translation: AAH20204.2 .
    BC039024 mRNA. Translation: AAH39024.1 . Sequence problems.
    BC041691 mRNA. Translation: AAH41691.1 .
    AF174602 Genomic DNA. Translation: AAF04523.1 .
    CCDSi CCDS10287.1. [Q8NEZ5-1 ]
    CCDS45310.1. [Q8NEZ5-3 ]
    RefSeqi NP_036302.1. NM_012170.3. [Q8NEZ5-3 ]
    NP_671717.1. NM_147188.2. [Q8NEZ5-1 ]
    UniGenei Hs.591115.

    3D structure databases

    ProteinModelPortali Q8NEZ5.
    SMRi Q8NEZ5. Positions 24-58.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117649. 7 interactions.
    IntActi Q8NEZ5. 2 interactions.
    STRINGi 9606.ENSP00000307833.

    PTM databases

    PhosphoSitei Q8NEZ5.

    Polymorphism databases

    DMDMi 30580428.

    Proteomic databases

    MaxQBi Q8NEZ5.
    PaxDbi Q8NEZ5.
    PRIDEi Q8NEZ5.

    Protocols and materials databases

    DNASUi 26263.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308275 ; ENSP00000307833 ; ENSG00000167196 . [Q8NEZ5-1 ]
    ENST00000453211 ; ENSP00000396442 ; ENSG00000167196 . [Q8NEZ5-3 ]
    ENST00000569022 ; ENSP00000457531 ; ENSG00000167196 . [Q8NEZ5-2 ]
    GeneIDi 26263.
    KEGGi hsa:26263.
    UCSCi uc002bbj.2. human. [Q8NEZ5-3 ]
    uc002bbk.3. human. [Q8NEZ5-1 ]

    Organism-specific databases

    CTDi 26263.
    GeneCardsi GC15P076196.
    HGNCi HGNC:13593. FBXO22.
    HPAi HPA003165.
    HPA050178.
    MIMi 609096. gene.
    neXtProti NX_Q8NEZ5.
    PharmGKBi PA28035.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG26261.
    HOGENOMi HOG000285967.
    HOVERGENi HBG051570.
    InParanoidi Q8NEZ5.
    KOi K10302.
    OMAi CCKVGAS.
    OrthoDBi EOG72G17J.
    PhylomeDBi Q8NEZ5.
    TreeFami TF328809.

    Miscellaneous databases

    GenomeRNAii 26263.
    NextBioi 48531.
    PROi Q8NEZ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8NEZ5.
    Bgeei Q8NEZ5.
    CleanExi HS_FBXO22.
    Genevestigatori Q8NEZ5.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    IPR019494. FIST_C_domain.
    [Graphical view ]
    Pfami PF00646. F-box. 1 hit.
    PF10442. FIST_C. 1 hit.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81383. SSF81383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "FBX22p44: a novel human F-box protein predominantly expressed in the liver."
      Tan P., Pan Z.-Q.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain, Colon and Lung.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
    6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "F-box and leucine-rich repeat protein 22 is a cardiac-enriched F-box protein that regulates sarcomeric protein turnover and is essential for maintenance of contractile function in vivo."
      Spaich S., Will R.D., Just S., Spaich S., Kuhn C., Frank D., Berger I.M., Wiemann S., Korn B., Koegl M., Backs J., Katus H.A., Rottbauer W., Frey N.
      Circ. Res. 111:1504-1516(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SKP1 AND CUL1.
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFBX22_HUMAN
    AccessioniPrimary (citable) accession number: Q8NEZ5
    Secondary accession number(s): Q0D2P8
    , Q6PIL5, Q8IXW3, Q9H824, Q9UKC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3