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Q8NEZ5 (FBX22_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box only protein 22
Alternative name(s):
F-box protein FBX22p44
Gene names
Name:FBXO22
Synonyms:FBX22
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function. Ref.9

Subunit structure

Directly interacts with SKP1 and CUL1. Ref.9

Subcellular location

CytoplasmmyofibrilsarcomereZ line By similarity.

Tissue specificity

Predominantly expressed in liver, also enriched in cardiac muscle. Ref.9

Sequence similarities

Contains 1 F-box domain.

Sequence caution

The sequence AAH39024.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NEZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NEZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     47-403: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q8NEZ5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     266-276: NPLDIDASGVV → YVLCASDFVCE
     277-403: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403F-box only protein 22
PRO_0000119906

Regions

Domain21 – 6747F-box

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6 Ref.10 Ref.11
Modified residue1941N6-acetyllysine Ref.7

Natural variations

Alternative sequence47 – 403357Missing in isoform 2.
VSP_041821
Alternative sequence266 – 27611NPLDIDASGVV → YVLCASDFVCE in isoform 3.
VSP_013058
Alternative sequence277 – 403127Missing in isoform 3.
VSP_013059

Experimental info

Sequence conflict4 – 118VGCCGECR → AGACGGP in AAF04523. Ref.5
Sequence conflict3491F → L in AAH32540. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D96712BAA1149D8D

FASTA40344,508
        10         20         30         40         50         60 
MEPVGCCGEC RGSSVDPRST FVLSNLAEVV ERVLTFLPAK ALLRVACVCR LWRECVRRVL 

        70         80         90        100        110        120 
RTHRSVTWIS AGLAEAGHLE GHCLVRVVAE ELENVRILPH TVLYMADSET FISLEECRGH 

       130        140        150        160        170        180 
KRARKRTSME TALALEKLFP KQCQVLGIVT PGIVVTPMGS GSNRPQEIEI GESGFALLFP 

       190        200        210        220        230        240 
QIEGIKIQPF HFIKDPKNLT LERHQLTEVG LLDNPELRVV LVFGYNCCKV GASNYLQQVV 

       250        260        270        280        290        300 
STFSDMNIIL AGGQVDNLSS LTSEKNPLDI DASGVVGLSF SGHRIQSATV LLNEDVSDEK 

       310        320        330        340        350        360 
TAEAAMQRLK AANIPEHNTI GFMFACVGRG FQYYRAKGNV EADAFRKFFP SVPLFGFFGN 

       370        380        390        400 
GEIGCDRIVT GNFILRKCNE VKDDDLFHSY TTIMALIHLG SSK 

« Hide

Isoform 2 [UniParc].

Checksum: 1D49258F07775FA2
Show »

FASTA464,922
Isoform 3 [UniParc].

Checksum: 0E9B0CE3E208358B
Show »

FASTA27630,589

References

« Hide 'large scale' references
[1]"FBX22p44: a novel human F-box protein predominantly expressed in the liver."
Tan P., Pan Z.-Q.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[3]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain, Colon and Lung.
[5]"Identification of a family of human F-box proteins."
Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M., Pagano M.
Curr. Biol. 9:1177-1179(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"F-box and leucine-rich repeat protein 22 is a cardiac-enriched F-box protein that regulates sarcomeric protein turnover and is essential for maintenance of contractile function in vivo."
Spaich S., Will R.D., Just S., Spaich S., Kuhn C., Frank D., Berger I.M., Wiemann S., Korn B., Koegl M., Backs J., Katus H.A., Rottbauer W., Frey N.
Circ. Res. 111:1504-1516(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SKP1 AND CUL1.
[10]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY005144 mRNA. Translation: AAF89095.1.
AK024048 mRNA. Translation: BAB14798.1.
AC027104 Genomic DNA. No translation available.
BC032540 mRNA. Translation: AAH32540.1.
BC020204 mRNA. Translation: AAH20204.2.
BC039024 mRNA. Translation: AAH39024.1. Sequence problems.
BC041691 mRNA. Translation: AAH41691.1.
AF174602 Genomic DNA. Translation: AAF04523.1.
RefSeqNP_036302.1. NM_012170.3.
NP_671717.1. NM_147188.2.
UniGeneHs.591115.

3D structure databases

ProteinModelPortalQ8NEZ5.
SMRQ8NEZ5. Positions 24-58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117649. 7 interactions.
IntActQ8NEZ5. 2 interactions.
STRING9606.ENSP00000307833.

PTM databases

PhosphoSiteQ8NEZ5.

Polymorphism databases

DMDM30580428.

Proteomic databases

PaxDbQ8NEZ5.
PRIDEQ8NEZ5.

Protocols and materials databases

DNASU26263.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308275; ENSP00000307833; ENSG00000167196. [Q8NEZ5-1]
ENST00000453211; ENSP00000396442; ENSG00000167196. [Q8NEZ5-3]
ENST00000540507; ENSP00000439270; ENSG00000167196.
ENST00000569022; ENSP00000457531; ENSG00000167196. [Q8NEZ5-2]
GeneID26263.
KEGGhsa:26263.
UCSCuc002bbj.2. human. [Q8NEZ5-3]
uc002bbk.3. human. [Q8NEZ5-1]

Organism-specific databases

CTD26263.
GeneCardsGC15P076196.
HGNCHGNC:13593. FBXO22.
HPAHPA003165.
HPA050178.
MIM609096. gene.
neXtProtNX_Q8NEZ5.
PharmGKBPA28035.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG26261.
HOGENOMHOG000285967.
HOVERGENHBG051570.
InParanoidQ8NEZ5.
KOK10302.
OMACCKVGAS.
OrthoDBEOG72G17J.
PhylomeDBQ8NEZ5.
TreeFamTF328809.

Gene expression databases

ArrayExpressQ8NEZ5.
BgeeQ8NEZ5.
CleanExHS_FBXO22.
GenevestigatorQ8NEZ5.

Family and domain databases

InterProIPR001810. F-box_dom.
IPR019494. FIST_C_domain.
[Graphical view]
PfamPF00646. F-box. 1 hit.
PF10442. FIST_C. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMSSF81383. SSF81383. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi26263.
NextBio48531.
PROQ8NEZ5.
SOURCESearch...

Entry information

Entry nameFBX22_HUMAN
AccessionPrimary (citable) accession number: Q8NEZ5
Secondary accession number(s): Q0D2P8 expand/collapse secondary AC list , Q6PIL5, Q8IXW3, Q9H824, Q9UKC0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM