Q8NEW0 (ZNT7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc transporter 7
Short name=ZnT-7

Alternative name(s):
Solute carrier family 30 member 7
Znt-like transporter 2

Gene names

Name:	SLC30A7
Synonyms:	ZNT7, ZNTL2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to facilitate zinc transport from the cytoplasm into the Golgi apparatus. Partly regulates cellular zinc homeostasis. Required with ZNT5 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and the vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT5 and ZNT6 for the activation of TNAP By similarity. Ref.2 Ref.9
Subunit structure	Homooligomer.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein Ref.1.
Induction	Increased intracellular zinc level, resulting from extracellular zinc supplementation, do not induce any up- or down-regulation of gene expression. Up-regulated by zinc depletion. Ref.2
Sequence similarities	Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sequestering of zinc ion Inferred from electronic annotation. Source: Compara transmembrane transport Traceable author statement. Source: Reactome zinc ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara vesicle Inferred from direct assay PubMed 17349999. Source: BHF-UCL
Molecular_function	cation transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD40	P25942	1	EBI-1043569,EBI-525714

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Zinc transporter 7		PRO_0000314299
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Topological domain	59 – 67	9	Extracellular Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 102	14	Cytoplasmic Potential
	Transmembrane	103 – 123	21	Helical; Potential
	Topological domain	124 – 140	17	Extracellular Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Topological domain	162 – 236	75	Cytoplasmic Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Topological domain	258 – 262	5	Extracellular Potential
	Transmembrane	263 – 283	21	Helical; Potential
	Topological domain	284 – 376	93	Cytoplasmic Potential
	Region	161 – 218	58	His-rich loop
Experimental info
	Sequence conflict	91	1	R → G in AAL83716. Ref.3
	Sequence conflict	318	1	V → A in AAL83716. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8NEW0 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 8E4230C4B9EC5848	FASTA	376	41,626
	10 20 30 40 50 60 MLPLSIKDDE YKPPKFNLFG KISGWFRSIL SDKTSRNLFF FLCLNLSFAF VELLYGIWSN 70 80 90 100 110 120 CLGLISDSFH MFFDSTAILA GLAASVISKW RDNDAFSYGY VRAEVLAGFV NGLFLIFTAF 130 140 150 160 170 180 FIFSEGVERA LAPPDVHHER LLLVSILGFV VNLIGIFVFK HGGHGHSHGS GHGHSHSLFN 190 200 210 220 230 240 GALDQAHGHV DHCHSHEVKH GAAHSHDHAH GHGHFHSHDG PSLKETTGPS RQILQGVFLH 250 260 270 280 290 300 ILADTLGSIG VIASAIMMQN FGLMIADPIC SILIAILIVV SVIPLLRESV GILMQRTPPL 310 320 330 340 350 360 LENSLPQCYQ RVQQLQGVYS LQEQHFWTLC SDVYVGTLKL IVAPDADARW ILSQTHNIFT 370 QAGVRQLYVQ IDFAAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ZnT7, a novel mammalian zinc transporter, accumulates zinc in the Golgi apparatus." Kirschke C.P., Huang L. J. Biol. Chem. 278:4096-4102(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"Differential regulation of zinc efflux transporters ZnT-1, ZnT-5 and ZnT-7 gene expression by zinc levels: a real-time RT-PCR study." Devergnas S., Chimienti F., Naud N., Pennequin A., Coquerel Y., Chantegrel J., Favier A., Seve M. Biochem. Pharmacol. 68:699-709(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[3]	"Cloning of new mammalian zinc transporter-like genes." Zhu W., Mager S. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trachea.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal kidney.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[9]	"Two different zinc transport complexes of cation diffusion facilitator proteins localized in the secretory pathway operate to activate alkaline phosphatases in vertebrate cells." Suzuki T., Ishihara K., Migaki H., Ishihara K., Nagao M., Yamaguchi-Iwai Y., Kambe T. J. Biol. Chem. 280:30956-30962(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF529197 mRNA. Translation: AAO17324.1. AY094606 mRNA. Translation: AAM21969.1. AF233345 mRNA. Translation: AAL83716.1. AK313638 mRNA. Translation: BAG36396.1. BX537375 mRNA. Translation: CAD97617.1. AL732465, AC104506 Genomic DNA. Translation: CAI13301.1. CH471097 Genomic DNA. Translation: EAW72942.1. CH471097 Genomic DNA. Translation: EAW72943.1. BC064692 mRNA. Translation: AAH64692.1.
IPI	IPI00302605.
RefSeq	NP_001138356.1. NM_001144884.1. NP_598003.2. NM_133496.4.
UniGene	Hs.533903.
3D structure databases
ProteinModelPortal	Q8NEW0.
SMR	Q8NEW0. Positions 41-134.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NEW0. 1 interaction.
STRING	9606.ENSP00000350278.
Protein family/group databases
TCDB	2.A.4.4.5. cation diffusion facilitator (CDF) family.
PTM databases
PhosphoSite	Q8NEW0.
Polymorphism databases
DMDM	74751261.
Proteomic databases
PaxDb	Q8NEW0.
PRIDE	Q8NEW0.
Protocols and materials databases
DNASU	148867.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357650; ENSP00000350278; ENSG00000162695. ENST00000370112; ENSP00000359130; ENSG00000162695.
GeneID	148867.
KEGG	hsa:148867.
UCSC	uc001dtn.2. human.
Organism-specific databases
CTD	148867.
GeneCards	GC01P101361.
HGNC	HGNC:19306. SLC30A7.
HPA	HPA018034.
MIM	611149. gene.
neXtProt	NX_Q8NEW0.
PharmGKB	PA134891595.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1230.
HOGENOM	HOG000018690.
HOVERGEN	HBG056637.
InParanoid	Q8NEW0.
KO	K14692.
OMA	FQHGGHG.
OrthoDB	EOG4W3SNC.
PhylomeDB	Q8NEW0.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q8NEW0.
Bgee	Q8NEW0.
CleanEx	HS_SLC30A7.
Genevestigator	Q8NEW0.
Family and domain databases
InterPro	IPR002524. Cation_efflux. [Graphical view]
PANTHER	PTHR11562. PTHR11562. 1 hit.
Pfam	PF01545. Cation_efflux. 1 hit. [Graphical view]
TIGRFAMs	TIGR01297. CDF. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	148867.
NextBio	86021.
SOURCE	Search...

Entry information

Entry name

ZNT7_HUMAN

Accession

Primary (citable) accession number: Q8NEW0
Secondary accession number(s): B2R949, D3DT61, Q8TCH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.