Q8NET6 (CHSTD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 13
EC=2.8.2.5

Alternative name(s):
Chondroitin 4-O-sulfotransferase 3
Chondroitin 4-sulfotransferase 3
Short name=C4ST-3
Short name=C4ST3

Gene names

Name:

CHST13

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan.
Catalytic activity	3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Highly expressed in adult liver. Expressed at lower level in kidney, lymph nodes and fetal kidney. Ref.1
Sequence similarities	Belongs to the sulfotransferase 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chondroitin sulfate biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	N-acetylgalactosamine 4-O-sulfotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB chondroitin 4-sulfotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Carbohydrate sulfotransferase 13		PRO_0000189671
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 341	311	Lumenal Potential
	Nucleotide binding	113 – 119	7	PAPS By similarity
	Nucleotide binding	174 – 182	9	PAPS By similarity
	Motif	132 – 134	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	146	1	P → S. Corresponds to variant rs34311016 [ dbSNP | Ensembl ].		VAR_053698
	Natural variant	271	1	A → V. Corresponds to variant rs1056523 [ dbSNP | Ensembl ].		VAR_021477
	Natural variant	317	1	R → Q. Corresponds to variant rs1056522 [ dbSNP | Ensembl ].		VAR_021478

Sequences

	Sequence		Length	Mass (Da)
	Q8NET6 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A372134675D661F6	FASTA	341	38,920
	10 20 30 40 50 60 MGRRCCRRRV LAAACLGAAL LLLCAAPRSL RPAFGNRALG SSWLGGEKRS PLQKLYDLDQ 70 80 90 100 110 120 DPRSTLAKVH RQRRDLLNSA CSRHSRRQRL LQPEDLRHVL VDDAHGLLYC YVPKVACTNW 130 140 150 160 170 180 KRVLLALSGQ ARGDPRAISA QEAHAPGRLP SLADFSPAEI NRRLRAYLAF LFVREPFERL 190 200 210 220 230 240 ASAYRNKLAR PYSAAFQRRY GARIVQRLRP RALPDARARG HDVRFAEFLA YLLDPRTRRE 250 260 270 280 290 300 EPFNEHWERA HALCHPCRLR YDVVGKFETL AEDAAFVLGL AGASDLSFPG PPRPRGAAAS 310 320 330 340 RDLAARLFRD ISPFYQRRLF DLYKMDFLLF NYSAPSYLRL L

References

[1]	"Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases." Kang H.-G., Evers M.R., Xia G., Baenziger J.U., Schachner M. J. Biol. Chem. 277:34766-34772(2002) [PubMed: 12080076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY120869 mRNA. Translation: AAM55481.1.
IPI	IPI00168745.
RefSeq	NP_690849.1. NM_152889.2.
UniGene	Hs.292375.
3D structure databases
ProteinModelPortal	Q8NET6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NET6.
PTM databases
PhosphoSite	Q8NET6.
Polymorphism databases
DMDM	61211841.
Proteomic databases
PRIDE	Q8NET6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319340; ENSP00000317404; ENSG00000180767.
GeneID	166012.
KEGG	hsa:166012.
UCSC	uc003eja.1. human.
Organism-specific databases
CTD	166012.
GeneCards	GC03P126243.
H-InvDB	HIX0030771.
HGNC	HGNC:21755. CHST13.
MIM	610124. gene.
neXtProt	NX_Q8NET6.
PharmGKB	PA134976803.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074373.
HOGENOM	HBG714587.
HOVERGEN	HBG050952.
InParanoid	Q8NET6.
OMA	HDVRFAE.
OrthoDB	EOG4RXZ0R.
PhylomeDB	Q8NET6.
Enzyme and pathway databases
BioCyc	MetaCyc:HS11524-MONOMER.
BRENDA	2.8.2.5. 2681.
Gene expression databases
ArrayExpress	Q8NET6.
Bgee	Q8NET6.
CleanEx	HS_CHST13.
Genevestigator	Q8NET6.
GermOnline	ENSG00000180767. Homo sapiens.
Family and domain databases
InterPro	IPR018011. Carb_sulfotransferase-rel. IPR005331. Sulfotransferase. [Graphical view]
KO	K07779.
PANTHER	PTHR12137. Carb_sulphotransferase. 1 hit.
Pfam	PF03567. Sulfotransfer_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	88576.
SOURCE	Search...

Entry information

Entry name

CHSTD_HUMAN

Accession

Primary (citable) accession number: Q8NET6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.