Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NFAT activation molecule 1

Gene

NFAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters. May be involved in the regulation of B-cell, but not T-cell, development. Overexpression activates downstream effectors without ligand binding or antibody cross-linking.2 Publications

GO - Molecular functioni

  • transmembrane signaling receptor activity Source: HGNC

GO - Biological processi

  • B cell differentiation Source: HGNC
  • cell surface receptor signaling pathway Source: InterPro
  • inflammatory response Source: HGNC
  • intracellular signal transduction Source: HGNC
  • neutrophil degranulation Source: Reactome
  • positive regulation of B cell receptor signaling pathway Source: HGNC
  • positive regulation of cytokine production Source: HGNC
  • positive regulation of DNA binding transcription factor activity Source: HGNC
  • regulation of B cell differentiation Source: InterPro
  • signal transduction Source: HGNC

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation
SignaLinkiQ8NET5

Names & Taxonomyi

Protein namesi
Recommended name:
NFAT activation molecule 1
Alternative name(s):
Calcineurin/NFAT-activating ITAM-containing protein
NFAT-activating protein with ITAM motif 1
Gene namesi
Name:NFAM1
Synonyms:CNAIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000235568.6
HGNCiHGNC:29872 NFAM1
MIMi608740 gene
neXtProtiNX_Q8NET5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 163ExtracellularSequence analysisAdd BLAST121
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 270CytoplasmicSequence analysisAdd BLAST86

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi220Y → A or F: Abolishes the ITAM-mediated-activating activity. 2 Publications1
Mutagenesisi231Y → A or F: Abolishes the ITAM-mediated-activating activity. 2 Publications1

Organism-specific databases

DisGeNETi150372
OpenTargetsiENSG00000235568
PharmGKBiPA134949664

Polymorphism and mutation databases

BioMutaiNFAM1
DMDMi34222694

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000001504643 – 270NFAT activation molecule 1Add BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi65 ↔ 114By similarity
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei220PhosphotyrosineCurated1
Modified residuei231PhosphotyrosineCurated1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8NET5
PaxDbiQ8NET5
PeptideAtlasiQ8NET5
PRIDEiQ8NET5

PTM databases

iPTMnetiQ8NET5
PhosphoSitePlusiQ8NET5

Expressioni

Tissue specificityi

Highly expressed in neutrophils, primary monocytes, mast cells, monocytic cell lines and lymphocytes. Also expressed in spleen B and T-cells, and lung. Expressed at low level in non-immune tissue.2 Publications

Gene expression databases

BgeeiENSG00000235568
CleanExiHS_NFAM1
ExpressionAtlasiQ8NET5 baseline and differential
GenevisibleiQ8NET5 HS

Organism-specific databases

HPAiHPA031812

Interactioni

Subunit structurei

No direct interaction with the B-cell antigen receptor (BCR). Interacts with SYK; probably involved in BCR signaling. Interacts with ZAP70 (By similarity).By similarity

Protein-protein interaction databases

BioGridi1272891 interactor.
IntActiQ8NET5 2 interactors.
STRINGi9606.ENSP00000333680

Structurei

3D structure databases

ProteinModelPortaliQ8NET5
SMRiQ8NET5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 150Ig-like V-typeAdd BLAST101
Domaini209 – 237ITAMPROSITE-ProRule annotationAdd BLAST29

Domaini

The ITAM domain displays no close similarity to any existing ITAMs, except for four conserved positions. The phosphorylated ITAM domain binds ZAP70 and SYK.

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J2TX Eukaryota
ENOG4111559 LUCA
GeneTreeiENSGT00390000000787
HOGENOMiHOG000113773
HOVERGENiHBG052611
InParanoidiQ8NET5
OMAiTEVYACI
OrthoDBiEOG091G0IQ5
PhylomeDBiQ8NET5
TreeFamiTF336307

Family and domain databases

InterProiView protein in InterPro
IPR033549 NFAM1
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR35680 PTHR35680, 1 hit
PROSITEiView protein in PROSITE
PS51055 ITAM_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NET5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQPVRWRA LPGLPRPPGL PAAPWLLLGV LLLPGTLRLA GGQSVTHTGL
60 70 80 90 100
PIMASLANTA ISFSCRITYP YTPQFKVFTV SYFHEDLQGQ RSPKKPTNCH
110 120 130 140 150
PGLGTENQSH TLDCQVTLVL PGASATGTYY CSVHWPHSTV RGSGTFILVR
160 170 180 190 200
DAGYREPPQS PQKLLLFGFT GLLSVLSVVG TALLLWNKKR MRGPGKDPTR
210 220 230 240 250
KCPDPRSASS PKQHPSESVY TALQRRETEV YACIENEDGS SPTAKQSPLS
260 270
QERPHRFEDD GELNLVYENL
Length:270
Mass (Da):29,686
Last modified:October 1, 2002 - v1
Checksum:i0026DBDE1FEAD2B3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049964137H → Y. Corresponds to variant dbSNP:rs34296033Ensembl.1
Natural variantiVAR_049965187N → K. Corresponds to variant dbSNP:rs17003048Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY247409 mRNA Translation: AAO91847.1
AY121370 mRNA Translation: AAM83133.1
CT841509 mRNA Translation: CAJ86439.1
AL022316 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW60505.1
BC038241 mRNA Translation: AAH38241.2
AK074094 mRNA Translation: BAB84920.1
CCDSiCCDS14034.1
RefSeqiNP_001305252.1, NM_001318323.1
NP_666017.1, NM_145912.6
UniGeneiHs.436677

Genome annotation databases

EnsembliENST00000329021; ENSP00000333680; ENSG00000235568
GeneIDi150372
KEGGihsa:150372
UCSCiuc003bcn.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNFAM1_HUMAN
AccessioniPrimary (citable) accession number: Q8NET5
Secondary accession number(s): B0QYD0
, Q20WL2, Q5JZ96, Q8IUY8, Q8TEM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: October 1, 2002
Last modified: February 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome