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Protein

Transient receptor potential cation channel subfamily V member 1

Gene

TRPV1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.By similarity4 Publications

Enzyme regulationi

Channel activity is activated via the interaction with PIRT and phosphatidylinositol 4,5-bisphosphate (PIP2). Both PIRT and PIP2 are required to activate channel activity (By similarity). The channel is sensitized by ATP binding. Repeated stimulation with capsaicin gives rise to progressively smaller responses, due to desensitization. This desensitization is triggered by the influx of calcium ions and is inhibited by elevated ATP levels. Ca2+ and CALM displace ATP from its binding site and trigger a conformation change that leads to a closed, desensitized channel. Intracellular PIP2 inhibits desensitization. The double-knot toxin (DkTx) from the Chinese earth tiger tarantula activates the channel and traps it in an open conformation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATPBy similarity1
Binding sitei156ATPBy similarity1
Binding sitei161ATPBy similarity1
Binding sitei165ATPBy similarity1
Binding sitei512AgonistBy similarity1
Binding sitei550AgonistBy similarity1
Sitei557Important for agonist bindingBy similarity1
Metal bindingi647Calcium; shared with neighboring subunitsBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi200 – 203ATPBy similarity4
Nucleotide bindingi211 – 212ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33720-MONOMER.
ReactomeiR-HSA-3295583. TRP channels.
SIGNORiQ8NER1.

Protein family/group databases

TCDBi1.A.4.2.13. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 1
Short name:
TrpV1
Alternative name(s):
Capsaicin receptor
Osm-9-like TRP channel 1
Short name:
OTRPC1
Vanilloid receptor 1
Gene namesi
Name:TRPV1
Synonyms:VR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:12716. TRPV1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 433CytoplasmicBy similarityAdd BLAST433
Transmembranei434 – 454HelicalBy similarityAdd BLAST21
Topological domaini455 – 471ExtracellularBy similarityAdd BLAST17
Transmembranei472 – 497HelicalBy similarityAdd BLAST26
Topological domaini498 – 510CytoplasmicBy similarityAdd BLAST13
Transmembranei511 – 531HelicalBy similarityAdd BLAST21
Topological domaini532 – 535ExtracellularBy similarity4
Transmembranei536 – 556HelicalBy similarityAdd BLAST21
Topological domaini557 – 571CytoplasmicBy similarityAdd BLAST15
Transmembranei572 – 599HelicalBy similarityAdd BLAST28
Topological domaini600 – 658ExtracellularBy similarityAdd BLAST59
Transmembranei659 – 687HelicalBy similarityAdd BLAST29
Topological domaini688 – 839CytoplasmicBy similarityAdd BLAST152

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi511Y → A: Loss of sensitivity to capsaicin. 1 Publication1
Mutagenesisi550T → I: Reduces sensitivity to capsaicin 40-fold. 1 Publication1

Organism-specific databases

DisGeNETi7442.
OpenTargetsiENSG00000196689.
PharmGKBiPA37329.

Chemistry databases

ChEMBLiCHEMBL4794.
DrugBankiDB00132. Alpha-Linolenic Acid.
DB00168. Aspartame.
DB06774. Capsaicin.
DB00159. Icosapent.
GuidetoPHARMACOLOGYi507.

Polymorphism and mutation databases

BioMutaiTRPV1.
DMDMi296452849.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153381 – 839Transient receptor potential cation channel subfamily V member 1Add BLAST839

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117Phosphoserine; by PKA and PKDBy similarity1
Modified residuei145Phosphothreonine; by PKA; in vitroBy similarity1
Modified residuei371Phosphothreonine; by PKA; in vitroBy similarity1
Modified residuei502Phosphoserine; by PKC/PRKCEBy similarity1
Glycosylationi604N-linked (GlcNAc...)By similarity1
Modified residuei705PhosphothreonineBy similarity1
Modified residuei775PhosphoserineBy similarity1
Modified residuei801Phosphoserine; by PKC/PRKCE and PKC/PRKCZBy similarity1
Modified residuei821PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8NER1.
PRIDEiQ8NER1.

PTM databases

iPTMnetiQ8NER1.
PhosphoSitePlusiQ8NER1.

Expressioni

Tissue specificityi

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000196689.
CleanExiHS_TRPV1.
ExpressionAtlasiQ8NER1. baseline and differential.
GenevisibleiQ8NER1. HS.

Interactioni

Subunit structurei

Interacts with PIRT (By similarity). Homotetramer (By similarity). Interacts with TRPV3 and may also form a heteromeric channel with TRPV3 (PubMed:12077606). Interacts with CALM, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity). Interacts with TMEM100 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • phosphoprotein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi113281. 7 interactors.
MINTiMINT-4721979.
STRINGi9606.ENSP00000459962.

Chemistry databases

BindingDBiQ8NER1.

Structurei

3D structure databases

ProteinModelPortaliQ8NER1.
SMRiQ8NER1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati111 – 153ANK 1Add BLAST43
Repeati154 – 200ANK 2Add BLAST47
Repeati201 – 247ANK 3Add BLAST47
Repeati248 – 283ANK 4Add BLAST36
Repeati284 – 332ANK 5Add BLAST49
Repeati333 – 359ANK 6Add BLAST27

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 116Important for channel activation by agonists and heatBy similarity2
Regioni685 – 713ADBy similarityAdd BLAST29
Regioni768 – 802Interaction with calmodulinBy similarityAdd BLAST35
Regioni778 – 793Required for PIP2-mediated channel inhibitionBy similarityAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi644 – 647Selectivity filterBy similarity4

Domaini

The association domain (AD) is necessary for self-association.By similarity

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234630.
HOVERGENiHBG054085.
InParanoidiQ8NER1.
KOiK05222.
PhylomeDBiQ8NER1.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008347. TRPV1-4_channel.
IPR024863. TRPV1_channel.
[Graphical view]
PANTHERiPTHR10582:SF17. PTHR10582:SF17. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01768. TRPVRECEPTOR.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NER1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKWSSTDLG AAADPLQKDT CPDPLDGDPN SRPPPAKPQL STAKSRTRLF
60 70 80 90 100
GKGDSEEAFP VDCPHEEGEL DSCPTITVSP VITIQRPGDG PTGARLLSQD
110 120 130 140 150
SVAASTEKTL RLYDRRSIFE AVAQNNCQDL ESLLLFLQKS KKHLTDNEFK
160 170 180 190 200
DPETGKTCLL KAMLNLHDGQ NTTIPLLLEI ARQTDSLKEL VNASYTDSYY
210 220 230 240 250
KGQTALHIAI ERRNMALVTL LVENGADVQA AAHGDFFKKT KGRPGFYFGE
260 270 280 290 300
LPLSLAACTN QLGIVKFLLQ NSWQTADISA RDSVGNTVLH ALVEVADNTA
310 320 330 340 350
DNTKFVTSMY NEILMLGAKL HPTLKLEELT NKKGMTPLAL AAGTGKIGVL
360 370 380 390 400
AYILQREIQE PECRHLSRKF TEWAYGPVHS SLYDLSCIDT CEKNSVLEVI
410 420 430 440 450
AYSSSETPNR HDMLLVEPLN RLLQDKWDRF VKRIFYFNFL VYCLYMIIFT
460 470 480 490 500
MAAYYRPVDG LPPFKMEKTG DYFRVTGEIL SVLGGVYFFF RGIQYFLQRR
510 520 530 540 550
PSMKTLFVDS YSEMLFFLQS LFMLATVVLY FSHLKEYVAS MVFSLALGWT
560 570 580 590 600
NMLYYTRGFQ QMGIYAVMIE KMILRDLCRF MFVYIVFLFG FSTAVVTLIE
610 620 630 640 650
DGKNDSLPSE STSHRWRGPA CRPPDSSYNS LYSTCLELFK FTIGMGDLEF
660 670 680 690 700
TENYDFKAVF IILLLAYVIL TYILLLNMLI ALMGETVNKI AQESKNIWKL
710 720 730 740 750
QRAITILDTE KSFLKCMRKA FRSGKLLQVG YTPDGKDDYR WCFRVDEVNW
760 770 780 790 800
TTWNTNVGII NEDPGNCEGV KRTLSFSLRS SRVSGRHWKN FALVPLLREA
810 820 830
SARDRQSAQP EEVYLRQFSG SLKPEDAEVF KSPAASGEK
Length:839
Mass (Da):94,956
Last modified:May 18, 2010 - v2
Checksum:i7142F59D428827FB
GO
Isoform 2 (identifier: Q8NER1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: MKKWSSTDLG...KKHLTDNEFK → METLTPGHLQ...ACPDPPLCLS

Show »
Length:837
Mass (Da):94,211
Checksum:iD662DD6094BDCA56
GO

Sequence cautioni

The sequence AAG43467 differs from that shown. Reason: Frameshift at position 498.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti336T → M in CAB89866 (PubMed:11226139).Curated1
Sequence conflicti552M → V in ABA06605 (Ref. 5) 1 Publication1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05730791P → S.1 PublicationCorresponds to variant rs222749dbSNPEnsembl.1
Natural variantiVAR_071244315M → I.8 PublicationsCorresponds to variant rs222747dbSNPEnsembl.1
Natural variantiVAR_057308469T → I.2 PublicationsCorresponds to variant rs224534dbSNPEnsembl.1
Natural variantiVAR_057309505T → A.Corresponds to variant rs17633288dbSNPEnsembl.1
Natural variantiVAR_022246585I → V.2 PublicationsCorresponds to variant rs8065080dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0568621 – 150MKKWS…DNEFK → METLTPGHLQPSPSSPRPRA APGSLGRVTRRRLSRWIALT RKVSWTPARPSQSALLSPSR GQETAPPVPGCCPRTLSPPA PRRPSGSMIAGVSLKPLLRI TARIWRACCSSCRRARSTSQ TTSSKVAPALGSGRAPALAC PDPPLCLS in isoform 2. 1 PublicationAdd BLAST150

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277028 mRNA. Translation: CAB95729.1.
AF196175 mRNA. Translation: AAG43466.1.
AF196176 mRNA. Translation: AAG43467.1. Frameshift.
AJ272063 mRNA. Translation: CAB89866.2.
AY131289 mRNA. Translation: AAM89472.1.
DQ177332 mRNA. Translation: ABA06605.1.
AL136801 mRNA. Translation: CAB66735.1.
AC027796 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90497.1.
BC132820 mRNA. Translation: AAI32821.1.
BC136633 mRNA. Translation: AAI36634.1.
CCDSiCCDS45576.1. [Q8NER1-1]
PIRiJC7621.
RefSeqiNP_061197.4. NM_018727.5. [Q8NER1-1]
NP_542435.2. NM_080704.3. [Q8NER1-1]
NP_542436.2. NM_080705.3. [Q8NER1-1]
NP_542437.2. NM_080706.3. [Q8NER1-1]
UniGeneiHs.579217.
Hs.655380.

Genome annotation databases

EnsembliENST00000399756; ENSP00000382659; ENSG00000196689. [Q8NER1-1]
ENST00000399759; ENSP00000382661; ENSG00000196689. [Q8NER1-1]
ENST00000571088; ENSP00000461007; ENSG00000196689. [Q8NER1-1]
ENST00000572705; ENSP00000459962; ENSG00000196689. [Q8NER1-1]
GeneIDi7442.
KEGGihsa:7442.
UCSCiuc010vrr.3. human. [Q8NER1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277028 mRNA. Translation: CAB95729.1.
AF196175 mRNA. Translation: AAG43466.1.
AF196176 mRNA. Translation: AAG43467.1. Frameshift.
AJ272063 mRNA. Translation: CAB89866.2.
AY131289 mRNA. Translation: AAM89472.1.
DQ177332 mRNA. Translation: ABA06605.1.
AL136801 mRNA. Translation: CAB66735.1.
AC027796 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90497.1.
BC132820 mRNA. Translation: AAI32821.1.
BC136633 mRNA. Translation: AAI36634.1.
CCDSiCCDS45576.1. [Q8NER1-1]
PIRiJC7621.
RefSeqiNP_061197.4. NM_018727.5. [Q8NER1-1]
NP_542435.2. NM_080704.3. [Q8NER1-1]
NP_542436.2. NM_080705.3. [Q8NER1-1]
NP_542437.2. NM_080706.3. [Q8NER1-1]
UniGeneiHs.579217.
Hs.655380.

3D structure databases

ProteinModelPortaliQ8NER1.
SMRiQ8NER1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113281. 7 interactors.
MINTiMINT-4721979.
STRINGi9606.ENSP00000459962.

Chemistry databases

BindingDBiQ8NER1.
ChEMBLiCHEMBL4794.
DrugBankiDB00132. Alpha-Linolenic Acid.
DB00168. Aspartame.
DB06774. Capsaicin.
DB00159. Icosapent.
GuidetoPHARMACOLOGYi507.

Protein family/group databases

TCDBi1.A.4.2.13. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

iPTMnetiQ8NER1.
PhosphoSitePlusiQ8NER1.

Polymorphism and mutation databases

BioMutaiTRPV1.
DMDMi296452849.

Proteomic databases

PaxDbiQ8NER1.
PRIDEiQ8NER1.

Protocols and materials databases

DNASUi7442.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399756; ENSP00000382659; ENSG00000196689. [Q8NER1-1]
ENST00000399759; ENSP00000382661; ENSG00000196689. [Q8NER1-1]
ENST00000571088; ENSP00000461007; ENSG00000196689. [Q8NER1-1]
ENST00000572705; ENSP00000459962; ENSG00000196689. [Q8NER1-1]
GeneIDi7442.
KEGGihsa:7442.
UCSCiuc010vrr.3. human. [Q8NER1-1]

Organism-specific databases

CTDi7442.
DisGeNETi7442.
GeneCardsiTRPV1.
H-InvDBHIX0013428.
HIX0079948.
HGNCiHGNC:12716. TRPV1.
MIMi602076. gene.
neXtProtiNX_Q8NER1.
OpenTargetsiENSG00000196689.
PharmGKBiPA37329.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234630.
HOVERGENiHBG054085.
InParanoidiQ8NER1.
KOiK05222.
PhylomeDBiQ8NER1.
TreeFamiTF314711.

Enzyme and pathway databases

BioCyciZFISH:G66-33720-MONOMER.
ReactomeiR-HSA-3295583. TRP channels.
SIGNORiQ8NER1.

Miscellaneous databases

GeneWikiiTRPV1.
GenomeRNAii7442.
PROiQ8NER1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196689.
CleanExiHS_TRPV1.
ExpressionAtlasiQ8NER1. baseline and differential.
GenevisibleiQ8NER1. HS.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008347. TRPV1-4_channel.
IPR024863. TRPV1_channel.
[Graphical view]
PANTHERiPTHR10582:SF17. PTHR10582:SF17. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01768. TRPVRECEPTOR.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV1_HUMAN
AccessioniPrimary (citable) accession number: Q8NER1
Secondary accession number(s): A2RUA9
, Q3LU47, Q9H0G9, Q9H303, Q9H304, Q9NQ74, Q9NY22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.