Q8NEN9 (PDZD8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PDZ domain-containing protein 8

Alternative name(s):
Sarcoma antigen NY-SAR-84/NY-SAR-104

Gene names

Name:	PDZD8
Synonyms:	PDZK8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the regulation of cell morphology and cytoskeletal organization. May inhibit herpes simplex virus 1 infection at an early stage. May promote retroviral infection. Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with MSN. Interacts with HIV-1 Gag polyprotein p55. Ref.7 Ref.9
Sequence similarities	Contains 1 PDZ (DHR) domain. Contains 1 phorbol-ester/DAG-type zinc finger.
Sequence caution	The sequence AAO65182.1 differs from that shown. Reason: Frameshift at position 421.

Ontologies

Keywords
Biological process	Host-virus interaction
Coding sequence diversity	Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoskeleton organization Inferred from mutant phenotype Ref.8. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell morphogenesis Inferred from mutant phenotype Ref.8. Source: UniProtKB virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1154	1154	PDZ domain-containing protein 8		PRO_0000058299
Regions
	Domain	366 – 449	84	PDZ
	Zinc finger	840 – 891	52	Phorbol-ester/DAG-type
	Coiled coil	1028 – 1063	36	Potential
	Compositional bias	551 – 626	76	Pro-rich
Amino acid modifications
	Modified residue	496	1	Phosphoserine Ref.5 Ref.6
	Modified residue	538	1	Phosphoserine Ref.5
Natural variations
	Natural variant	806	1	V → A. Corresponds to variant rs35664484 [ dbSNP | Ensembl ].		VAR_051265
	Natural variant	897	1	R → Q. Corresponds to variant rs363294 [ dbSNP | Ensembl ].		VAR_051266
Experimental info
	Sequence conflict	232	1	V → G in AAO65182. Ref.3
	Sequence conflict	482	1	A → T in AAO65182. Ref.3
	Sequence conflict	488	1	D → N in AAO65182. Ref.3
	Sequence conflict	491	1	S → K in AAO65182. Ref.3
	Sequence conflict	666	1	S → F in CAB59184. Ref.4
	Sequence conflict	1007 – 1008	2	GL → RG in AAO65177. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8NEN9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B09263C483645B13	FASTA	1,154	128,563
	10 20 30 40 50 60 MGLLLMILAS AVLGSFLTLL AQFFLLYRRQ PEPPADEAAR AGEGFRYIKP VPGLLLREYL 70 80 90 100 110 120 YGGGRDEEPS GAAPEGGATP TAAPETPAPP TRETCYFLNA TILFLFRELR DTALTRRWVT 130 140 150 160 170 180 KKIKVEFEEL LQTKTAGRLL EGLSLRDVFL GETVPFIKTI RLVRPVVPSA TGEPDGPEGE 190 200 210 220 230 240 ALPAACPEEL AFEAEVEYNG GFHLAIDVDL VFGKSAYLFV KLSRVVGRLR LVFTRVPFTH 250 260 270 280 290 300 WFFSFVEDPL IDFEVRSQFE GRPMPQLTSI IVNQLKKIIK RKHTLPNYKI RFKPFFPYQT 310 320 330 340 350 360 LQGFEEDEEH IHIQQWALTE GRLKVTLLEC SRLLIFGSYD REANVHCTLE LSSSVWEEKQ 370 380 390 400 410 420 RSSIKTVELI KGNLQSVGLT LRLVQSTDGY AGHVIIETVA PNSPAAIADL QRGDRLIAIG 430 440 450 460 470 480 GVKITSTLQV LKLIKQAGDR VLVYYERPVG QSNQGAVLQD NFGQLEENFL SSSCQSGYEE 490 500 510 520 530 540 EAAGLTVDTE SRELDSEFED LASDVRAQNE FKDEAQSLSH SPKRVPTTLS IKPLGAISPV 550 560 570 580 590 600 LNRKLAVGSH PLPPKIQSKD GNKPPPLKTS EITDPAQVSK PTQGSAFKPP VPPRPQAKVP 610 620 630 640 650 660 LPSADAPNQA EPDVLVEKPE KVVPPPLVDK SAEKQAKNVD AIDDAAAPKQ FLAKQEVAKD 670 680 690 700 710 720 VTSETSCPTK DSSDDRQTWE SSEILYRNKL GKWTRTRASC LFDIEACHRY LNIALWCRDP 730 740 750 760 770 780 FKLGGLICLG HVSLKLEDVA LGCLATSNTE YLSKLRLEAP SPKAIVTRTA LRNLSMQKGF 790 800 810 820 830 840 NDKFCYGDIT IHFKYLKEGE SDHHVVTNVE KEKEPHLVEE VSVLPKEEQF VGQMGLTENK 850 860 870 880 890 900 HSFQDTQFQN PTWCDYCKKK VWTKAASQCM FCAYVCHKKC QEKCLAETSV CGATDRRIDR 910 920 930 940 950 960 TLKNLRLEGQ ETLLGLPPRV DAEASKSVNK TTGLTRHIIN TSSRLLNLRQ VSKTRLSEPG 970 980 990 1000 1010 1020 TDLVEPSPKH TPNTSDNEGS DTEVCGPNSP SKRGNSTGIK LVRKEGGLDD SVFIAVKEIG 1030 1040 1050 1060 1070 1080 RDLYRGLPTE ERIQKLEFML DKLQNEIDQE LEHNNSLVRE EKETTDTRKK SLLSAALAKS 1090 1100 1110 1120 1130 1140 GERLQALTLL MIHYRAGIED IETLESLSLD QHSKKISKYT DDTEEDLDNE ISQLIDSQPF 1150 SSISDDLFGP SESV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Immunomic analysis of human sarcoma." Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 232-491 AND 1007-1154.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 642-1154. Tissue: Testis.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-496 AND SER-538, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-496, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"PDZD8 is a novel Gag-interacting factor that promotes retroviral infection." Henning M.S., Morham S.G., Goff S.P., Naghavi M.H. J. Virol. 84:8990-8995(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HIV-1 GAG.
[8]	"Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration." Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J. BMC Biol. 9:54-54(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"PDZD8 is a novel moesin-interacting cytoskeletal regulatory protein that suppresses infection by herpes simplex virus type 1." Henning M.S., Stiedl P., Barry D.S., McMahon R., Morham S.G., Walsh D., Naghavi M.H. Virology 415:114-121(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MSN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL391988, AL359836 Genomic DNA. Translation: CAH70347.1. AL359836, AL391988 Genomic DNA. Translation: CAH73253.1. BC028375 mRNA. Translation: AAH28375.1. AY211924 mRNA. Translation: AAO65177.1. AY211929 mRNA. Translation: AAO65182.1. Frameshift. AL122051 mRNA. Translation: CAB59184.1.
IPI	IPI00168698.
PIR	T34546.
RefSeq	NP_776152.1. NM_173791.3.
UniGene	Hs.501149.
3D structure databases
ProteinModelPortal	Q8NEN9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000334642.
PTM databases
PhosphoSite	Q8NEN9.
Polymorphism databases
DMDM	73621383.
Proteomic databases
PaxDb	Q8NEN9.
PRIDE	Q8NEN9.
Protocols and materials databases
DNASU	118987.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334464; ENSP00000334642; ENSG00000165650.
GeneID	118987.
KEGG	hsa:118987.
UCSC	uc001lde.1. human.
Organism-specific databases
CTD	118987.
GeneCards	GC10M119033.
HGNC	HGNC:26974. PDZD8.
HPA	HPA015716.
MIM	614235. gene.
neXtProt	NX_Q8NEN9.
PharmGKB	PA134916380.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG292931.
HOGENOM	HOG000090230.
HOVERGEN	HBG082118.
InParanoid	Q8NEN9.
OMA	VFIAVKE.
OrthoDB	EOG4QVCBF.
PhylomeDB	Q8NEN9.
Gene expression databases
Bgee	Q8NEN9.
CleanEx	HS_PDZD8.
Genevestigator	Q8NEN9.
GermOnline	ENSG00000165650. Homo sapiens.
Family and domain databases
InterPro	IPR001478. PDZ. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF13180. PDZ_2. 1 hit. [Graphical view]
SMART	SM00109. C1. 1 hit. SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50106. PDZ. 1 hit. PS00479. ZF_DAG_PE_1. False negative. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PDZD8. human.
GenomeRNAi	118987.
NextBio	80380.
SOURCE	Search...

Entry information

Entry name

PDZD8_HUMAN

Accession

Primary (citable) accession number: Q8NEN9
Secondary accession number(s): Q86WE0, Q86WE5, Q9UFF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.