Reviewed, UniProtKB/Swiss-Prot Q8NEM8 (CBPC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Cytosolic carboxypeptidase 3
    EC=3.4.17.-
Alternative name(s):
    ATP/GTP-binding protein-like 3

Gene names

Name:	AGBL3
Synonyms:	CCP3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1001 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the processing of tubulin By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallocarboxypeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NEM8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NEM8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     614-621: SSRGSDSS → RELTFLLR
     622-1001: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NEM8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: W → GVSCWSESHQAGAQWCDLGSLQPPPPRFKRFACFSLLSSRDYRRVPPRPANFVFLVETGFHHVGQDGLRLLTS
     106-1001: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1001	1001	Cytosolic carboxypeptidase 3		PRO_0000283751
Sites
	Active site	414	1	Nucleophile By similarity
	Metal binding	364	1	Zinc By similarity
	Metal binding	367	1	Zinc By similarity
	Metal binding	460	1	Zinc By similarity
Amino acid modifications
	Modified residue	733	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	105	1	W → GVSCWSESHQAGAQWCDLGS LQPPPPRFKRFACFSLLSSR DYRRVPPRPANFVFLVETGF HHVGQDGLRLLTS in isoform 3.		VSP_024370
	Alternative sequence	106 – 1001	896	Missing in isoform 3.		VSP_024371
	Alternative sequence	614 – 621	8	SSRGSDSS → RELTFLLR in isoform 2.		VSP_024372
	Alternative sequence	622 – 1001	380	Missing in isoform 2.		VSP_024373
	Natural variant	45	1	F → Y: dbSNP rs2348049.		VAR_031573
	Natural variant	122	1	E → Q: dbSNP rs4236655. Ref.3		VAR_031574
	Natural variant	360	1	T → I: dbSNP rs17804854.		VAR_031575

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 4846AEF2F76336AF	FASTA	1,001	116,011
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDWTPSCP EPVYIPTGLE 130 140 150 160 170 180 TEPLYPDSKE ATVVYLAEDA YKEPCFVYSR VGGNRTPLKQ PVDYRDNTLM FEARFESGNL 190 200 210 220 230 240 QKVVKVAEYE YQLTVRPDLF TNKHTQWYYF QVTNMRAGIV YRFTIVNFTK PASLYSRGMR 250 260 270 280 290 300 PLFYSEKEAK AHHIGWQRIG DQIKYYRNNP GQDGRHYFSL TWTFQFPHNK DTCYFAHCYP 310 320 330 340 350 360 YTYTNLQEYL SGINNDPVRS KFCKIRVLCH TLARNMVYIL TITTPLKNSD SRKRKAVILT 370 380 390 400 410 420 ARVHPGETNS SWIMKGFLDY ILGNSSDAQL LRDTFVFKVV PMLNPDGVIV GNYRCSLAGR 430 440 450 460 470 480 DLNRNYTSLL KESFPSVWYT RNMVHRLMEK REVILYCDLH GHSRKENIFM YGCDGSDRSK 490 500 510 520 530 540 TLYLQQRIFP LMLSKNCPDK FSFSACKFNV QKSKEGTGRV VMWKMGIRNS FTMEATFCGS 550 560 570 580 590 600 TLGNKRGTHF STKDLESMGY HFCDSLLDYC DPDRTKYYRC LKELEEMERH ITLEKVFEDS 610 620 630 640 650 660 DTPVIDITLD VESSSRGSDS SESIDSLTYL LKLTSQKKHL KTKKERNSTI ASHQNARGQE 670 680 690 700 710 720 VYDRGHLLQR HTQSNSDVKD TRPNEPDDYM VDYFRRQLPN QGLAHCKLRL PGSRHSPASA 730 740 750 760 770 780 SRVAGTTGTR HHTWLIFVFL VEMGKKIPLK GTDLYGNCFK VTSLQSPMGK QTSTWTEKTR 790 800 810 820 830 840 IPTEDLHHNL KSKIKECISF QSKKTGINWT DDEKRSYKDK GIVQTQEILQ YLLPIVHSTK 850 860 870 880 890 900 NMQTTQIKQL FNPRTNFQIQ HQLNPATCRN IKKYSTSWTA PRNHPFVIQG DVMANSSEWV 910 920 930 940 950 960 QSKPHRSLES LSPLKGPKKN KHSQIWAIKN EDIKPLSSKW ETASSSFGMD ANVLKYKSLQ 970 980 990 1000 AEETNQQSSK HTALHLTKNK DEQANKNDGQ PTLYLKFQRE S
	Isoform 2. Checksum: 37FA956BD0142C8B	FASTA	621	72,650
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDWTPSCP EPVYIPTGLE 130 140 150 160 170 180 TEPLYPDSKE ATVVYLAEDA YKEPCFVYSR VGGNRTPLKQ PVDYRDNTLM FEARFESGNL 190 200 210 220 230 240 QKVVKVAEYE YQLTVRPDLF TNKHTQWYYF QVTNMRAGIV YRFTIVNFTK PASLYSRGMR 250 260 270 280 290 300 PLFYSEKEAK AHHIGWQRIG DQIKYYRNNP GQDGRHYFSL TWTFQFPHNK DTCYFAHCYP 310 320 330 340 350 360 YTYTNLQEYL SGINNDPVRS KFCKIRVLCH TLARNMVYIL TITTPLKNSD SRKRKAVILT 370 380 390 400 410 420 ARVHPGETNS SWIMKGFLDY ILGNSSDAQL LRDTFVFKVV PMLNPDGVIV GNYRCSLAGR 430 440 450 460 470 480 DLNRNYTSLL KESFPSVWYT RNMVHRLMEK REVILYCDLH GHSRKENIFM YGCDGSDRSK 490 500 510 520 530 540 TLYLQQRIFP LMLSKNCPDK FSFSACKFNV QKSKEGTGRV VMWKMGIRNS FTMEATFCGS 550 560 570 580 590 600 TLGNKRGTHF STKDLESMGY HFCDSLLDYC DPDRTKYYRC LKELEEMERH ITLEKVFEDS 610 620 DTPVIDITLD VESRELTFLL R
	Isoform 3. Checksum: EC834FFDAF0A5400	FASTA	177	20,403
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDGVSCWS ESHQAGAQWC 130 140 150 160 170 DLGSLQPPPP RFKRFACFSL LSSRDYRRVP PRPANFVFLV ETGFHHVGQD GLRLLTS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-122. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK023045 mRNA. Translation: BAB14371.1. AC083862 Genomic DNA. No translation available. AC083870 Genomic DNA. No translation available. AC090497 Genomic DNA. No translation available. BC030651 mRNA. Translation: AAH30651.1.
IPI	IPI00302490. IPI00303972. IPI00843995.
UniGene	Hs.648616
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M14.026.
PTM databases
PhosphoSite	Q8NEM8.
Genome annotation databases
Ensembl	ENST00000458078; ENSP00000395969; ENSG00000146856; Homo sapiens. [Genome view]
Organism-specific databases
GeneCards	GC07P134321.
HGNC	HGNC:27981. AGBL3.
HPA	HPA020397. HPA024150.
PharmGKB	PA142672635.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8NEM8.
OMA	KRIYRDK
OrthoDB	EOG9QRKPV
Gene expression databases
ArrayExpress	Q8NEM8.
Bgee	Q8NEM8.
CleanEx	HS_AGBL3.
Genevestigator	Q8NEM8.
Family and domain databases
InterPro	IPR000834. Peptidase_M14. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	97830.

Entry information

Entry name

CBPC3_HUMAN

Accession

Primary (citable) accession number: Q8NEM8
Secondary accession number(s): Q9H965

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	November 24, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.