Q8NEM8 (CBPC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic carboxypeptidase 3
EC=3.4.17.-

Alternative name(s):
ATP/GTP-binding protein-like 3

Gene names

Name:	AGBL3
Synonyms:	CCP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1001 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metallocarboxypeptidase that may play a role in the processing of tubulin. Ref.4
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallocarboxypeptidase activity Inferred from direct assay Ref.4. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NEM8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NEM8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     614-621: SSRGSDSS → RELTFLLR
     622-1001: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NEM8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: W → GVSCWSESHQAGAQWCDLGSLQPPPPRFKRFACFSLLSSRDYRRVPPRPANFVFLVETGFHHVGQDGLRLLTS
     106-1001: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8NEM8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     704-784: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1001	1001	Cytosolic carboxypeptidase 3		PRO_0000283751
Sites
	Active site	414	1	Nucleophile By similarity
	Metal binding	364	1	Zinc By similarity
	Metal binding	367	1	Zinc By similarity
	Metal binding	460	1	Zinc By similarity
Natural variations
	Alternative sequence	105	1	W → GVSCWSESHQAGAQWCDLGS LQPPPPRFKRFACFSLLSSR DYRRVPPRPANFVFLVETGF HHVGQDGLRLLTS in isoform 3.		VSP_024370
	Alternative sequence	106 – 1001	896	Missing in isoform 3.		VSP_024371
	Alternative sequence	614 – 621	8	SSRGSDSS → RELTFLLR in isoform 2.		VSP_024372
	Alternative sequence	622 – 1001	380	Missing in isoform 2.		VSP_024373
	Alternative sequence	704 – 784	81	Missing in isoform 4.		VSP_040423
	Natural variant	45	1	F → Y. Corresponds to variant rs2348049 [ dbSNP | Ensembl ].		VAR_031573
	Natural variant	122	1	E → Q. Ref.1 Ref.3 Corresponds to variant rs4236655 [ dbSNP | Ensembl ].		VAR_031574
	Natural variant	360	1	T → I. Corresponds to variant rs17804854 [ dbSNP | Ensembl ].		VAR_031575
Experimental info
	Sequence conflict	210	1	F → S in BAH13813. Ref.1
	Sequence conflict	396	1	V → A in BAH13813. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 4846AEF2F76336AF	FASTA	1,001	116,011
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDWTPSCP EPVYIPTGLE 130 140 150 160 170 180 TEPLYPDSKE ATVVYLAEDA YKEPCFVYSR VGGNRTPLKQ PVDYRDNTLM FEARFESGNL 190 200 210 220 230 240 QKVVKVAEYE YQLTVRPDLF TNKHTQWYYF QVTNMRAGIV YRFTIVNFTK PASLYSRGMR 250 260 270 280 290 300 PLFYSEKEAK AHHIGWQRIG DQIKYYRNNP GQDGRHYFSL TWTFQFPHNK DTCYFAHCYP 310 320 330 340 350 360 YTYTNLQEYL SGINNDPVRS KFCKIRVLCH TLARNMVYIL TITTPLKNSD SRKRKAVILT 370 380 390 400 410 420 ARVHPGETNS SWIMKGFLDY ILGNSSDAQL LRDTFVFKVV PMLNPDGVIV GNYRCSLAGR 430 440 450 460 470 480 DLNRNYTSLL KESFPSVWYT RNMVHRLMEK REVILYCDLH GHSRKENIFM YGCDGSDRSK 490 500 510 520 530 540 TLYLQQRIFP LMLSKNCPDK FSFSACKFNV QKSKEGTGRV VMWKMGIRNS FTMEATFCGS 550 560 570 580 590 600 TLGNKRGTHF STKDLESMGY HFCDSLLDYC DPDRTKYYRC LKELEEMERH ITLEKVFEDS 610 620 630 640 650 660 DTPVIDITLD VESSSRGSDS SESIDSLTYL LKLTSQKKHL KTKKERNSTI ASHQNARGQE 670 680 690 700 710 720 VYDRGHLLQR HTQSNSDVKD TRPNEPDDYM VDYFRRQLPN QGLAHCKLRL PGSRHSPASA 730 740 750 760 770 780 SRVAGTTGTR HHTWLIFVFL VEMGKKIPLK GTDLYGNCFK VTSLQSPMGK QTSTWTEKTR 790 800 810 820 830 840 IPTEDLHHNL KSKIKECISF QSKKTGINWT DDEKRSYKDK GIVQTQEILQ YLLPIVHSTK 850 860 870 880 890 900 NMQTTQIKQL FNPRTNFQIQ HQLNPATCRN IKKYSTSWTA PRNHPFVIQG DVMANSSEWV 910 920 930 940 950 960 QSKPHRSLES LSPLKGPKKN KHSQIWAIKN EDIKPLSSKW ETASSSFGMD ANVLKYKSLQ 970 980 990 1000 AEETNQQSSK HTALHLTKNK DEQANKNDGQ PTLYLKFQRE S
	Isoform 2 [UniParc]. Checksum: 37FA956BD0142C8B	FASTA	621	72,650
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDWTPSCP EPVYIPTGLE 130 140 150 160 170 180 TEPLYPDSKE ATVVYLAEDA YKEPCFVYSR VGGNRTPLKQ PVDYRDNTLM FEARFESGNL 190 200 210 220 230 240 QKVVKVAEYE YQLTVRPDLF TNKHTQWYYF QVTNMRAGIV YRFTIVNFTK PASLYSRGMR 250 260 270 280 290 300 PLFYSEKEAK AHHIGWQRIG DQIKYYRNNP GQDGRHYFSL TWTFQFPHNK DTCYFAHCYP 310 320 330 340 350 360 YTYTNLQEYL SGINNDPVRS KFCKIRVLCH TLARNMVYIL TITTPLKNSD SRKRKAVILT 370 380 390 400 410 420 ARVHPGETNS SWIMKGFLDY ILGNSSDAQL LRDTFVFKVV PMLNPDGVIV GNYRCSLAGR 430 440 450 460 470 480 DLNRNYTSLL KESFPSVWYT RNMVHRLMEK REVILYCDLH GHSRKENIFM YGCDGSDRSK 490 500 510 520 530 540 TLYLQQRIFP LMLSKNCPDK FSFSACKFNV QKSKEGTGRV VMWKMGIRNS FTMEATFCGS 550 560 570 580 590 600 TLGNKRGTHF STKDLESMGY HFCDSLLDYC DPDRTKYYRC LKELEEMERH ITLEKVFEDS 610 620 DTPVIDITLD VESRELTFLL R
	Isoform 3 [UniParc]. Checksum: EC834FFDAF0A5400	FASTA	177	20,403
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDGVSCWS ESHQAGAQWC 130 140 150 160 170 DLGSLQPPPP RFKRFACFSL LSSRDYRRVP PRPANFVFLV ETGFHHVGQD GLRLLTS
	Isoform 4 [UniParc]. Checksum: 804684527132B7C8	FASTA	920	107,047
	10 20 30 40 50 60 MSEDSEKEDY SDRTISDEDE SDEDMFMKFV SEDLHRCALL TADSFGDPFF PRTTQILLEY 70 80 90 100 110 120 QLGRWVPRLR EPRDLYGVSS SGPLSPTRWP YHCEVIDEKV QHIDWTPSCP EPVYIPTGLE 130 140 150 160 170 180 TEPLYPDSKE ATVVYLAEDA YKEPCFVYSR VGGNRTPLKQ PVDYRDNTLM FEARFESGNL 190 200 210 220 230 240 QKVVKVAEYE YQLTVRPDLF TNKHTQWYYF QVTNMRAGIV YRFTIVNFTK PASLYSRGMR 250 260 270 280 290 300 PLFYSEKEAK AHHIGWQRIG DQIKYYRNNP GQDGRHYFSL TWTFQFPHNK DTCYFAHCYP 310 320 330 340 350 360 YTYTNLQEYL SGINNDPVRS KFCKIRVLCH TLARNMVYIL TITTPLKNSD SRKRKAVILT 370 380 390 400 410 420 ARVHPGETNS SWIMKGFLDY ILGNSSDAQL LRDTFVFKVV PMLNPDGVIV GNYRCSLAGR 430 440 450 460 470 480 DLNRNYTSLL KESFPSVWYT RNMVHRLMEK REVILYCDLH GHSRKENIFM YGCDGSDRSK 490 500 510 520 530 540 TLYLQQRIFP LMLSKNCPDK FSFSACKFNV QKSKEGTGRV VMWKMGIRNS FTMEATFCGS 550 560 570 580 590 600 TLGNKRGTHF STKDLESMGY HFCDSLLDYC DPDRTKYYRC LKELEEMERH ITLEKVFEDS 610 620 630 640 650 660 DTPVIDITLD VESSSRGSDS SESIDSLTYL LKLTSQKKHL KTKKERNSTI ASHQNARGQE 670 680 690 700 710 720 VYDRGHLLQR HTQSNSDVKD TRPNEPDDYM VDYFRRQLPN QGLDLHHNLK SKIKECISFQ 730 740 750 760 770 780 SKKTGINWTD DEKRSYKDKG IVQTQEILQY LLPIVHSTKN MQTTQIKQLF NPRTNFQIQH 790 800 810 820 830 840 QLNPATCRNI KKYSTSWTAP RNHPFVIQGD VMANSSEWVQ SKPHRSLESL SPLKGPKKNK 850 860 870 880 890 900 HSQIWAIKNE DIKPLSSKWE TASSSFGMDA NVLKYKSLQA EETNQQSSKH TALHLTKNKD 910 920 EQANKNDGQP TLYLKFQRES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), VARIANT GLN-122. Tissue: Testis.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLN-122. Tissue: Testis.
[4]	"Nna1-like proteins are active metallocarboxypeptidases of a new and diverse M14 subfamily." Rodriguez de la Vega M., Sevilla R.G., Hermoso A., Lorenzo J., Tanco S., Diez A., Fricker L.D., Bautista J.M., Aviles F.X. FASEB J. 21:851-865(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK023045 mRNA. Translation: BAB14371.1. AK302827 mRNA. Translation: BAH13813.1. AC083862 Genomic DNA. No translation available. AC083870 Genomic DNA. No translation available. AC090497 Genomic DNA. No translation available. BC030651 mRNA. Translation: AAH30651.1.
IPI	IPI00302490. IPI00303972. IPI00843995. IPI00921808.
RefSeq	NP_848658.3. NM_178563.3.
UniGene	Hs.648616.
3D structure databases
ProteinModelPortal	Q8NEM8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000388275.
Protein family/group databases
MEROPS	M14.026.
PTM databases
PhosphoSite	Q8NEM8.
Polymorphism databases
DMDM	143955274.
Proteomic databases
PaxDb	Q8NEM8.
PRIDE	Q8NEM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000275763; ENSP00000275763; ENSG00000146856. ENST00000436302; ENSP00000388275; ENSG00000146856.
GeneID	340351.
KEGG	hsa:340351.
UCSC	uc011kpw.2. human.
Organism-specific databases
CTD	340351.
GeneCards	GC07P134671.
HGNC	HGNC:27981. AGBL3.
HPA	HPA020397. HPA024150.
neXtProt	NX_Q8NEM8.
PharmGKB	PA142672635.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2866.
HOGENOM	HOG000111052.
HOVERGEN	HBG070840.
InParanoid	Q8NEM8.
Gene expression databases
ArrayExpress	Q8NEM8.
Bgee	Q8NEM8.
CleanEx	HS_AGBL3.
Genevestigator	Q8NEM8.
Family and domain databases
InterPro	IPR000834. Peptidase_M14. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	340351.
NextBio	97830.

Entry information

Entry name

CBPC3_HUMAN

Accession

Primary (citable) accession number: Q8NEM8
Secondary accession number(s): B7Z827, Q9H965

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.