Q8NEJ9 (NGDN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuroguidin

Alternative name(s):
EIF4E-binding protein

Gene names

Name:	NGDN
Synonyms:	C14orf120

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the translational repression of cytoplasmic polyadenylation element (CPE)-containing mRNAs By similarity.
Subunit structure	Interacts with CPEB1 and EIF4E By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cell projection › axon By similarity. Cell projection › dendrite By similarity. Cell projection › filopodium By similarity. Note: Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites By similarity.
Sequence similarities	Belongs to the SAS10 family.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cell projection Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	axon Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from electronic annotation. Source: UniProtKB-SubCell filopodium Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NEJ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NEJ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     311-311: F → Q
     312-315: Missing.

Note: May be due to a competing donor splice site.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 315	314	Neuroguidin		PRO_0000114331
Regions
	Region	41 – 174	134	Necessary for interaction with EIF4E By similarity
	Coiled coil	13 – 41	29	Potential
	Coiled coil	181 – 205	25	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.7
	Modified residue	142	1	Phosphoserine Ref.5
	Modified residue	143	1	Phosphoserine Ref.5 Ref.6
	Modified residue	202	1	Phosphoserine Ref.6
	Modified residue	204	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	311	1	F → Q in isoform 2.		VSP_009935
	Alternative sequence	312 – 315	4	Missing in isoform 2.		VSP_009936
	Natural variant	15	1	V → L. Corresponds to variant rs10149626 [ dbSNP | Ensembl ].		VAR_051898
	Natural variant	308	1	K → I. Corresponds to variant rs17093050 [ dbSNP | Ensembl ].		VAR_051899

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: F5B26C3C2EDDC840	FASTA	315	35,894
	10 20 30 40 50 60 MAALGVLESD LPSAVTLLKN LQEQVMAVTA QVKSLTQKVQ AGAYPTEKGL SFLEVKDQLL 70 80 90 100 110 120 LMYLMDLTHL ILDKASGGSL QGHDAVLRLV EIRTVLEKLR PLDQKLKYQI DKLIKTAVTG 130 140 150 160 170 180 SLSENDPLRF KPHPSNMMSK LSSEDEEEDE AEDDQSEASG KKSVKGVSKK YVPPRLVPVH 190 200 210 220 230 240 YDETEAEREK KRLERAKRRA LSSSVIRELK EQYSDAPEEI RDARHPHVTR QSQEDQHRIN 250 260 270 280 290 300 YEESMMVRLS VSKREKGRRK RANVMSSQLH SLTHFSDISA LTGGTVHLDE DQNPIKKRKK 310 IPQKGRKKKG FRRRR
	Isoform 2 [UniParc]. Checksum: F1CDC840E2968036	FASTA	311	35,250
	10 20 30 40 50 60 MAALGVLESD LPSAVTLLKN LQEQVMAVTA QVKSLTQKVQ AGAYPTEKGL SFLEVKDQLL 70 80 90 100 110 120 LMYLMDLTHL ILDKASGGSL QGHDAVLRLV EIRTVLEKLR PLDQKLKYQI DKLIKTAVTG 130 140 150 160 170 180 SLSENDPLRF KPHPSNMMSK LSSEDEEEDE AEDDQSEASG KKSVKGVSKK YVPPRLVPVH 190 200 210 220 230 240 YDETEAEREK KRLERAKRRA LSSSVIRELK EQYSDAPEEI RDARHPHVTR QSQEDQHRIN 250 260 270 280 290 300 YEESMMVRLS VSKREKGRRK RANVMSSQLH SLTHFSDISA LTGGTVHLDE DQNPIKKRKK 310 IPQKGRKKKG Q

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[5]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND SER-143, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[6]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143; SER-202 AND SER-204, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK291875 mRNA. Translation: BAF84564.1. AL050003 mRNA. Translation: CAB43232.2. CH471078 Genomic DNA. Translation: EAW66149.1. BC030817 mRNA. Translation: AAH30817.1.
IPI	IPI00000162. IPI00410360.
PIR	T08694.
RefSeq	NP_001036100.1. NM_001042635.1. NP_056329.1. NM_015514.1.
UniGene	Hs.9043.
3D structure databases
ProteinModelPortal	Q8NEJ9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NEJ9.
PTM databases
PhosphoSite	Q8NEJ9.
Polymorphism databases
DMDM	46395905.
Proteomic databases
PRIDE	Q8NEJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000408901; ENSP00000386134; ENSG00000129460.
GeneID	25983.
KEGG	hsa:25983.
UCSC	uc001wjy.1. human. uc001wjz.1. human.
Organism-specific databases
CTD	25983.
GeneCards	GC14P023924.
H-InvDB	HIX0202051.
HGNC	HGNC:20271. NGDN.
HPA	HPA000944.
MIM	610777. gene.
neXtProt	NX_Q8NEJ9.
PharmGKB	PA134957933.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12033.
GeneTree	ENSGT00500000044922.
HOGENOM	HBG314366.
HOVERGEN	HBG082025.
InParanoid	Q8NEJ9.
OMA	QEDQHRI.
PhylomeDB	Q8NEJ9.
Gene expression databases
ArrayExpress	Q8NEJ9.
Bgee	Q8NEJ9.
CleanEx	HS_NGDN.
Genevestigator	Q8NEJ9.
GermOnline	ENSG00000129460. Homo sapiens.
Family and domain databases
InterPro	IPR007146. Sas10/Utp3/C1D. [Graphical view]
KO	K14765.
Pfam	PF04000. Sas10_Utp3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	47646.
SOURCE	Search...

Entry information

Entry name

NGDN_HUMAN

Accession

Primary (citable) accession number: Q8NEJ9
Secondary accession number(s): A8K760, Q9Y400

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.