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Protein

Dual specificity protein phosphatase 18

Gene

DUSP18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation2 Publications
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.2 Publications

Enzyme regulationi

Activated by manganese ions, inhibited by iodoaretic acid.1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  • phosphatase activity Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:HS09503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 18 (EC:3.1.3.162 Publications, EC:3.1.3.482 Publications)
Alternative name(s):
Low molecular weight dual specificity phosphatase 201 Publication
Short name:
LMW-DSP20
Gene namesi
Name:DUSP18
Synonyms:LMWDSP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:18484. DUSP18.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Intermembrane side By similarity

  • Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73D → A: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi102L → V: No effect on in vitro phosphatase activity. 1 Publication1
Mutagenesisi104C → S: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi110R → K: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi111S → A: Abolishes most of in vitro phosphatase activity. 1 Publication1

Organism-specific databases

DisGeNETi150290.
OpenTargetsiENSG00000167065.
PharmGKBiPA134928498.

Polymorphism and mutation databases

BioMutaiDUSP18.
DMDMi29840768.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948281 – 188Dual specificity protein phosphatase 18Add BLAST188

Proteomic databases

MaxQBiQ8NEJ0.
PaxDbiQ8NEJ0.
PRIDEiQ8NEJ0.

PTM databases

DEPODiQ8NEJ0.
PhosphoSitePlusiQ8NEJ0.

Expressioni

Tissue specificityi

Widely expressed with highest levels in liver, brain, ovary and testis.2 Publications

Gene expression databases

BgeeiENSG00000167065.
CleanExiHS_DUSP18.
ExpressionAtlasiQ8NEJ0. baseline and differential.
GenevisibleiQ8NEJ0. HS.

Organism-specific databases

HPAiCAB034070.
HPA051349.

Interactioni

Protein-protein interaction databases

BioGridi127280. 1 interactor.
IntActiQ8NEJ0. 1 interactor.
STRINGi9606.ENSP00000333917.

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 24Combined sources4
Beta strandi27 – 30Combined sources4
Helixi34 – 36Combined sources3
Helixi38 – 43Combined sources6
Beta strandi48 – 51Combined sources4
Beta strandi65 – 68Combined sources4
Helixi79 – 82Combined sources4
Helixi83 – 95Combined sources13
Beta strandi100 – 103Combined sources4
Beta strandi105 – 109Combined sources5
Helixi110 – 122Combined sources13
Helixi127 – 137Combined sources11
Helixi145 – 159Combined sources15
Helixi176 – 178Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ESBX-ray2.00A1-188[»]
ProteinModelPortaliQ8NEJ0.
SMRiQ8NEJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NEJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 149Tyrosine-protein phosphataseAdd BLAST70

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1718. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ8NEJ0.
KOiK14165.
OMAiHVQAIRP.
OrthoDBiEOG091G0249.
PhylomeDBiQ8NEJ0.
TreeFamiTF316009.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NEJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPSCAFPV QFRQPSVSGL SQITKSLYIS NGVAANNKLM LSSNQITMVI
60 70 80 90 100
NVSVEVVNTL YEDIQYMQVP VADSPNSRLC DFFDPIADHI HSVEMKQGRT
110 120 130 140 150
LLHCAAGVSR SAALCLAYLM KYHAMSLLDA HTWTKSCRPI IRPNSGFWEQ
160 170 180
LIHYEFQLFG KNTVHMVSSP VGMIPDIYEK EVRLMIPL
Length:188
Mass (Da):21,066
Last modified:October 1, 2002 - v1
Checksum:iA6EDECC3624F570F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAN77931 (PubMed:12591617).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533017 mRNA. Translation: AAN59787.1.
AF461689 mRNA. Translation: AAN77931.1.
CR456406 mRNA. Translation: CAG30292.1.
AK056074 mRNA. Translation: BAG51616.1.
CH471095 Genomic DNA. Translation: EAW59913.1.
BC030987 mRNA. Translation: AAH30987.1.
CCDSiCCDS13883.1.
RefSeqiNP_001291723.1. NM_001304794.1.
NP_001291724.1. NM_001304795.1.
NP_689724.3. NM_152511.4.
XP_005261425.1. XM_005261368.4.
XP_006724211.1. XM_006724148.3.
XP_011528222.1. XM_011529920.2.
XP_011528223.1. XM_011529921.2.
XP_016884116.1. XM_017028627.1.
XP_016884117.1. XM_017028628.1.
UniGeneiHs.517544.

Genome annotation databases

EnsembliENST00000334679; ENSP00000333917; ENSG00000167065.
ENST00000377087; ENSP00000366291; ENSG00000167065.
ENST00000404885; ENSP00000385463; ENSG00000167065.
ENST00000407308; ENSP00000386063; ENSG00000167065.
GeneIDi150290.
KEGGihsa:150290.
UCSCiuc003aiu.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533017 mRNA. Translation: AAN59787.1.
AF461689 mRNA. Translation: AAN77931.1.
CR456406 mRNA. Translation: CAG30292.1.
AK056074 mRNA. Translation: BAG51616.1.
CH471095 Genomic DNA. Translation: EAW59913.1.
BC030987 mRNA. Translation: AAH30987.1.
CCDSiCCDS13883.1.
RefSeqiNP_001291723.1. NM_001304794.1.
NP_001291724.1. NM_001304795.1.
NP_689724.3. NM_152511.4.
XP_005261425.1. XM_005261368.4.
XP_006724211.1. XM_006724148.3.
XP_011528222.1. XM_011529920.2.
XP_011528223.1. XM_011529921.2.
XP_016884116.1. XM_017028627.1.
XP_016884117.1. XM_017028628.1.
UniGeneiHs.517544.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ESBX-ray2.00A1-188[»]
ProteinModelPortaliQ8NEJ0.
SMRiQ8NEJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127280. 1 interactor.
IntActiQ8NEJ0. 1 interactor.
STRINGi9606.ENSP00000333917.

PTM databases

DEPODiQ8NEJ0.
PhosphoSitePlusiQ8NEJ0.

Polymorphism and mutation databases

BioMutaiDUSP18.
DMDMi29840768.

Proteomic databases

MaxQBiQ8NEJ0.
PaxDbiQ8NEJ0.
PRIDEiQ8NEJ0.

Protocols and materials databases

DNASUi150290.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334679; ENSP00000333917; ENSG00000167065.
ENST00000377087; ENSP00000366291; ENSG00000167065.
ENST00000404885; ENSP00000385463; ENSG00000167065.
ENST00000407308; ENSP00000386063; ENSG00000167065.
GeneIDi150290.
KEGGihsa:150290.
UCSCiuc003aiu.4. human.

Organism-specific databases

CTDi150290.
DisGeNETi150290.
GeneCardsiDUSP18.
HGNCiHGNC:18484. DUSP18.
HPAiCAB034070.
HPA051349.
MIMi611446. gene.
neXtProtiNX_Q8NEJ0.
OpenTargetsiENSG00000167065.
PharmGKBiPA134928498.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1718. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ8NEJ0.
KOiK14165.
OMAiHVQAIRP.
OrthoDBiEOG091G0249.
PhylomeDBiQ8NEJ0.
TreeFamiTF316009.

Enzyme and pathway databases

BioCyciZFISH:HS09503-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8NEJ0.
GeneWikiiDUSP18.
GenomeRNAii150290.
PROiQ8NEJ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167065.
CleanExiHS_DUSP18.
ExpressionAtlasiQ8NEJ0. baseline and differential.
GenevisibleiQ8NEJ0. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS18_HUMAN
AccessioniPrimary (citable) accession number: Q8NEJ0
Secondary accession number(s): B3KPA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.