Q8NEG5 (ZSWM2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase ZSWIM2
EC=6.3.2.-

Alternative name(s):
MEKK1-related protein X
Short name=MEX
ZZ-type zinc finger-containing protein 2
Zinc finger SWIM domain-containing protein 2

Gene names

Name:	ZSWIM2
Synonyms:	ZZZ2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase involved in the regulation of Fas-, DR3- and DR4-mediated apoptosis. Functions in conjunction with the UBE2D1, UBE2D3 and UBE2E1 E2 ubiquitin-conjugating enzymes By similarity.
Subunit structure	Dimer By similarity. Interacts with UBE2D1 By similarity.
Domain	The SWIM-type zinc finger is reqiered for ubiquitination activity By similarity.
Post-translational modification	Polyubiquitinated. Polyubiquitination is followed by degradation via the proteasome By similarity.
Sequence similarities	Contains 2 RING-type zinc fingers. Contains 1 SWIM-type zinc finger. Contains 1 ZZ-type zinc finger.

Ontologies

Keywords
Biological process	Apoptosis Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW protein polyubiquitination Inferred from electronic annotation. Source: Compara regulation of apoptotic process Inferred from electronic annotation. Source: Compara
Molecular_function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 633

633

E3 ubiquitin-protein ligase ZSWIM2

PRO_0000223097

Regions

Zinc finger

54 – 87

34

SWIM-type

Zinc finger

147 – 198

52

RING-type 1

Zinc finger

228 – 277

50

ZZ-type

Zinc finger

344 – 388

45

RING-type 2

Experimental info

Sequence conflict

88

1

K → E in AAH31094. Ref.4

Sequence conflict

265

1

C → Y in AAH31094. Ref.4

Sequence conflict

302

1

I → T in AAH31094. Ref.4

Sequence conflict

385

1

D → G in AAH31094. Ref.4

Sequence conflict

486

1

D → E in AAH31094. Ref.4

Sequence conflict

537

1

F → Y in AAH31094. Ref.4

Sequence conflict

544

1

M → V in AAH31094. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

633

Helix Strand Turn

Details...

Beta strand

235 – 237

3

Beta strand

246 – 254

9

Beta strand

256 – 258

3

Helix

259 – 263

5

Helix

267 – 269

3

Beta strand

272 – 274

3

Beta strand

277 – 279

3

Sequences

	Sequence		Length	Mass (Da)
	Q8NEG5 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: FB5B74E5E7FA625C	FASTA	633	72,732
	10 20 30 40 50 60 MLRRGYKASE RRRHLSERLS WHQDQALSSS IYLLREMGPT GFLLREEEPE YMDFRVFLGN 70 80 90 100 110 120 PHVCNCSTFP KGGELCKHIC WVLLKKFKLP RNHESALQLG LGEREISDLL RGIHRVQTPQ 130 140 150 160 170 180 PGTNDENEHV EEDGYIKQKE IDSEDICSIC QELLLEKKLP VTFCRFGCGN SIHIKCMKIL 190 200 210 220 230 240 ANYQSTSNTS MLKCPLCRKE FAPLKLILEE FKNSSKLVAA AEKERLDKHL GIPCNNCKQF 250 260 270 280 290 300 PIEGKCYKCT ECIEYHLCQE CFDSCCHLSH TFTFREKRNQ KWRSLEKRAD EVVKYIDTKN 310 320 330 340 350 360 EIEEKMSHFQ EKQGQVYTPK HIVRSLPLQL ITKNSKLLAP GYQCLLCLKA FHLGQHTRLL 370 380 390 400 410 420 PCTHKFHRKC IDNWLFHKCN SCPIDGQVIY NPLTWKNSAV NGQAHQSVSN RDIIHLSKQK 430 440 450 460 470 480 EPDLFIPGTG LVLKQNRLGI LPSIPQCNFD ELNTPQSPKD AYENTTIDNL CSIKLDNSNS 490 500 510 520 530 540 KKLTYDYKIS QHFPRYLQDL PTVSFGKIPS QTLLPPIVHK NIVCPTAMES PCISGKFHTS 550 560 570 580 590 600 LSRMTKGCKC NNHNLKKTPA TKIREDNKRS TLLPEDFNLI VNWSTAKLSL SKRYSNCMGE 610 620 630 ITRKCSHLSR QPVSHSVNTK STELSLIIEG VQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Solution structure of the ZZ domain of zinc finger SWIM domain containing protein 2." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2009) to the PDB data bank Cited for: STRUCTURE BY NMR OF 208-292.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK128006 mRNA. Translation: BAG54618.1.
AC018735 Genomic DNA. Translation: AAY14907.1.
AC103914 Genomic DNA. Translation: AAX82016.1.
CH471058 Genomic DNA. Translation: EAX10929.1.
BC031094 mRNA. Translation: AAH31094.1.

IPI

IPI00302370.

RefSeq

NP_872327.2. NM_182521.2.

UniGene

Hs.375054.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DIP	NMR	-	A	208-292	[»]

ProteinModelPortal

Q8NEG5.

SMR

Q8NEG5. Positions 142-202, 208-292, 341-389.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8NEG5. 2 interactions.

MINT

MINT-1465934.

PTM databases

PhosphoSite

Q8NEG5.

Polymorphism databases

DMDM

41018471.

Proteomic databases

PaxDb

Q8NEG5.

PRIDE

Q8NEG5.

Protocols and materials databases

DNASU

151112.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000295131; ENSP00000295131; ENSG00000163012.

GeneID

151112.

KEGG

hsa:151112.

UCSC

uc002upu.1. human.

Organism-specific databases

CTD

151112.

GeneCards

GC02M187693.

HGNC

HGNC:30990. ZSWIM2.

HPA

HPA036418.

neXtProt

NX_Q8NEG5.

PharmGKB

PA134900131.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG241067.

HOGENOM

HOG000065782.

HOVERGEN

HBG060888.

InParanoid

Q8NEG5.

KO

K15716.

OMA

QVIYNPL.

OrthoDB

EOG4BK53B.

PhylomeDB

Q8NEG5.

Gene expression databases

ArrayExpress

Q8NEG5.

Bgee

Q8NEG5.

CleanEx

HS_ZSWIM2.

Genevestigator

Q8NEG5.

GermOnline

ENSG00000163012. Homo sapiens.

Family and domain databases

Gene3D

3.30.40.10. 2 hits.

InterPro

IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR007527. Znf_SWIM.
IPR000433. Znf_ZZ.
[Graphical view]

Pfam

PF04434. SWIM. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]

SMART

SM00184. RING. 2 hits.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]

PROSITE

PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 2 hits.
PS50966. ZF_SWIM. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8NEG5.

GenomeRNAi

151112.

NextBio

86587.

Entry information

Entry name

ZSWM2_HUMAN

Accession

Primary (citable) accession number: Q8NEG5
Secondary accession number(s): B3KXV6, Q53SI3, Q57ZY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.