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Protein

Phospholipid phosphatase 5

Gene

PLPP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol. May be a metastatic suppressor for hepatocellular carcinoma.2 Publications

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=41 µM for diacylglycerol pyrophosphate1 Publication
  2. KM=277 µM for phosphatidate1 Publication
  3. KM=295 µM for lysophosphatidate1 Publication

    GO - Molecular functioni

    • phosphatidate phosphatase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BRENDAi3.1.3.4. 2681.
    3.1.3.81. 2681.
    ReactomeiR-HSA-2029485. Role of phospholipids in phagocytosis.

    Chemistry databases

    SwissLipidsiSLP:000000604.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipid phosphatase 5Imported (EC:3.1.3.4)
    Alternative name(s):
    Phosphatidic acid phosphatase type 2 domain-containing protein 1B
    Gene namesi
    Name:PLPP5Imported
    Synonyms:DPPL1, HTPAP, PPAPDC1B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:25026. PLPP5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
    Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
    Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
    Transmembranei180 – 200HelicalSequence analysisAdd BLAST21
    Transmembranei209 – 228HelicalSequence analysisAdd BLAST20

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi115R → A: Loss of phosphatase activity; when associated with A-152 and with A-208. 1 Publication1
    Mutagenesisi152H → A: Loss of phosphatase activity; when associated with A-115 and with A-208. 1 Publication1
    Mutagenesisi208H → A: Loss of phosphatase activity; when associated with A-115 and with A-152. 1 Publication1

    Keywords - Diseasei

    Tumor suppressor

    Organism-specific databases

    DisGeNETi84513.
    OpenTargetsiENSG00000147535.
    PharmGKBiPA142671154.

    Polymorphism and mutation databases

    BioMutaiPPAPDC1B.
    DMDMi313104170.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002869451 – 264Phospholipid phosphatase 5Add BLAST264

    Proteomic databases

    EPDiQ8NEB5.
    MaxQBiQ8NEB5.
    PaxDbiQ8NEB5.
    PeptideAtlasiQ8NEB5.
    PRIDEiQ8NEB5.

    PTM databases

    DEPODiQ8NEB5.
    iPTMnetiQ8NEB5.
    PhosphoSitePlusiQ8NEB5.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    BgeeiENSG00000147535.
    CleanExiHS_PPAPDC1B.
    ExpressionAtlasiQ8NEB5. baseline and differential.
    GenevisibleiQ8NEB5. HS.

    Organism-specific databases

    HPAiHPA055777.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8NEB5. 1 interactor.
    STRINGi9606.ENSP00000392553.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NEB5.
    SMRiQ8NEB5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni108 – 116Phosphatase sequence motif I9
    Regioni149 – 152Phosphatase sequence motif II4
    Regioni201 – 211Phosphatase sequence motif IIIAdd BLAST11

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3030. Eukaryota.
    COG0671. LUCA.
    GeneTreeiENSGT00510000046723.
    HOGENOMiHOG000206608.
    HOVERGENiHBG056764.
    InParanoidiQ8NEB5.
    OMAiEMWLYKF.
    OrthoDBiEOG091G0O0I.
    PhylomeDBiQ8NEB5.
    TreeFamiTF323722.

    Family and domain databases

    Gene3Di1.20.144.10. 1 hit.
    InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
    IPR032462. PPAPDC1B.
    [Graphical view]
    PANTHERiPTHR10165:SF87. PTHR10165:SF87. 1 hit.
    PfamiPF01569. PAP2. 1 hit.
    [Graphical view]
    SMARTiSM00014. acidPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48317. SSF48317. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8NEB5-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGKAAAAVAF GAEVGVRLAL FAAFLVTELL PPFQRLIQPE EMWLYRNPYV
    60 70 80 90 100
    EAEYFPTKPM FVIAFLSPLS LIFLAKFLKK ADTRDSRQAC LAASLALALN
    110 120 130 140 150
    GVFTNTIKLI VGRPRPDFFY RCFPDGLAHS DLMCTGDKDV VNEGRKSFPS
    160 170 180 190 200
    GHSSFAFAGL AFASFYLAGK LHCFTPQGRG KSWRFCAFLS PLLFAAVIAL
    210 220 230 240 250
    SRTCDYKHHW QDVLVGSMIG MTFAYVCYRQ YYPPLTDAEC HKPFQDKLVL
    260
    STAQKPGDSY CFDI
    Length:264
    Mass (Da):29,484
    Last modified:November 30, 2010 - v2
    Checksum:i3682DA52B6F3C65B
    GO
    Isoform 2 (identifier: Q8NEB5-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         212-216: DVLVG → GPFKW
         217-264: Missing.

    Show »
    Length:216
    Mass (Da):24,091
    Checksum:i64D0637A9E546E5A
    GO
    Isoform 3 (identifier: Q8NEB5-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-41: Missing.

    Show »
    Length:223
    Mass (Da):25,159
    Checksum:i72B01C9A0DBBA1BA
    GO
    Isoform 4 (identifier: Q8NEB5-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         155-176: FAFAGLAFASFYLAGKLHCFTP → CMNIIWTLPYACFHIIEFNLES
         177-264: Missing.

    Note: No experimental confirmation available. Gene prediction based on EST data.
    Show »
    Length:176
    Mass (Da):19,677
    Checksum:iC0D4D05D0E7BE789
    GO

    Sequence cautioni

    The sequence AAI06015 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence AAK14924 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0400551 – 41Missing in isoform 3. 1 PublicationAdd BLAST41
    Alternative sequenceiVSP_054076155 – 176FAFAG…HCFTP → CMNIIWTLPYACFHIIEFNL ES in isoform 4. CuratedAdd BLAST22
    Alternative sequenceiVSP_054077177 – 264Missing in isoform 4. CuratedAdd BLAST88
    Alternative sequenceiVSP_025241212 – 216DVLVG → GPFKW in isoform 2. 2 Publications5
    Alternative sequenceiVSP_025242217 – 264Missing in isoform 2. 2 PublicationsAdd BLAST48

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BD418664 mRNA. No translation available.
    AC087362 Genomic DNA. No translation available.
    BC033025 mRNA. Translation: AAH33025.1.
    BC106014 mRNA. Translation: AAI06015.2. Different initiation.
    AF212238 mRNA. Translation: AAK14924.1. Different initiation.
    CCDSiCCDS47841.1. [Q8NEB5-1]
    CCDS47842.1. [Q8NEB5-2]
    CCDS47843.1. [Q8NEB5-4]
    RefSeqiNP_001096029.1. NM_001102559.1. [Q8NEB5-1]
    NP_001096030.1. NM_001102560.1. [Q8NEB5-4]
    NP_115872.2. NM_032483.3. [Q8NEB5-2]
    XP_011542973.1. XM_011544671.1. [Q8NEB5-3]
    UniGeneiHs.567619.

    Genome annotation databases

    EnsembliENST00000419686; ENSP00000414522; ENSG00000147535. [Q8NEB5-4]
    ENST00000422581; ENSP00000390622; ENSG00000147535. [Q8NEB5-2]
    ENST00000424479; ENSP00000392553; ENSG00000147535. [Q8NEB5-1]
    ENST00000529359; ENSP00000434916; ENSG00000147535. [Q8NEB5-3]
    GeneIDi84513.
    KEGGihsa:84513.
    UCSCiuc003xle.5. human. [Q8NEB5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BD418664 mRNA. No translation available.
    AC087362 Genomic DNA. No translation available.
    BC033025 mRNA. Translation: AAH33025.1.
    BC106014 mRNA. Translation: AAI06015.2. Different initiation.
    AF212238 mRNA. Translation: AAK14924.1. Different initiation.
    CCDSiCCDS47841.1. [Q8NEB5-1]
    CCDS47842.1. [Q8NEB5-2]
    CCDS47843.1. [Q8NEB5-4]
    RefSeqiNP_001096029.1. NM_001102559.1. [Q8NEB5-1]
    NP_001096030.1. NM_001102560.1. [Q8NEB5-4]
    NP_115872.2. NM_032483.3. [Q8NEB5-2]
    XP_011542973.1. XM_011544671.1. [Q8NEB5-3]
    UniGeneiHs.567619.

    3D structure databases

    ProteinModelPortaliQ8NEB5.
    SMRiQ8NEB5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ8NEB5. 1 interactor.
    STRINGi9606.ENSP00000392553.

    Chemistry databases

    SwissLipidsiSLP:000000604.

    PTM databases

    DEPODiQ8NEB5.
    iPTMnetiQ8NEB5.
    PhosphoSitePlusiQ8NEB5.

    Polymorphism and mutation databases

    BioMutaiPPAPDC1B.
    DMDMi313104170.

    Proteomic databases

    EPDiQ8NEB5.
    MaxQBiQ8NEB5.
    PaxDbiQ8NEB5.
    PeptideAtlasiQ8NEB5.
    PRIDEiQ8NEB5.

    Protocols and materials databases

    DNASUi84513.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000419686; ENSP00000414522; ENSG00000147535. [Q8NEB5-4]
    ENST00000422581; ENSP00000390622; ENSG00000147535. [Q8NEB5-2]
    ENST00000424479; ENSP00000392553; ENSG00000147535. [Q8NEB5-1]
    ENST00000529359; ENSP00000434916; ENSG00000147535. [Q8NEB5-3]
    GeneIDi84513.
    KEGGihsa:84513.
    UCSCiuc003xle.5. human. [Q8NEB5-1]

    Organism-specific databases

    CTDi84513.
    DisGeNETi84513.
    GeneCardsiPPAPDC1B.
    H-InvDBHIX0201275.
    HGNCiHGNC:25026. PLPP5.
    HPAiHPA055777.
    MIMi610626. gene.
    neXtProtiNX_Q8NEB5.
    OpenTargetsiENSG00000147535.
    PharmGKBiPA142671154.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3030. Eukaryota.
    COG0671. LUCA.
    GeneTreeiENSGT00510000046723.
    HOGENOMiHOG000206608.
    HOVERGENiHBG056764.
    InParanoidiQ8NEB5.
    OMAiEMWLYKF.
    OrthoDBiEOG091G0O0I.
    PhylomeDBiQ8NEB5.
    TreeFamiTF323722.

    Enzyme and pathway databases

    BRENDAi3.1.3.4. 2681.
    3.1.3.81. 2681.
    ReactomeiR-HSA-2029485. Role of phospholipids in phagocytosis.

    Miscellaneous databases

    ChiTaRSiPPAPDC1B. human.
    GenomeRNAii84513.
    PROiQ8NEB5.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000147535.
    CleanExiHS_PPAPDC1B.
    ExpressionAtlasiQ8NEB5. baseline and differential.
    GenevisibleiQ8NEB5. HS.

    Family and domain databases

    Gene3Di1.20.144.10. 1 hit.
    InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
    IPR032462. PPAPDC1B.
    [Graphical view]
    PANTHERiPTHR10165:SF87. PTHR10165:SF87. 1 hit.
    PfamiPF01569. PAP2. 1 hit.
    [Graphical view]
    SMARTiSM00014. acidPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48317. SSF48317. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPLPP5_HUMAN
    AccessioniPrimary (citable) accession number: Q8NEB5
    Secondary accession number(s): C9JKF5, Q3KQX6, Q9BY45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: November 30, 2010
    Last modified: November 2, 2016
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.