Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8NE09 (RGS22_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 22

Short name=RGS22
Gene names
Name:RGS22
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1264 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form By similarity.

Subunit structure

Interacts with GNA11, GNA12 AND GNA13. Ref.1

Subcellular location

Cytoplasm. Nucleus. Note: Expressed in the cytoplasm of spermatogonia and spermatocytes. In spermatids, also expressed in the nucleus. Ref.1

Tissue specificity

Testis-specific. Expressed in Leydig cells and spermatogenic cells from the spermatogonia to spermatid stages (at protein level). Ref.1

Sequence similarities

Contains 2 RGS domains.

Sequence caution

The sequence AAG49397.1 differs from that shown. Reason: Frameshift at positions 877, 882, 886 and 891.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NE09-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NE09-2)

The sequence of this isoform differs from the canonical sequence as follows:
     505-1264: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q8NE09-3)

The sequence of this isoform differs from the canonical sequence as follows:
     187-198: Missing.
Note: May be due to competing donor splice site.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12641264Regulator of G-protein signaling 22
PRO_0000271376

Regions

Domain852 – 980129RGS 1
Domain1021 – 1145125RGS 2
Coiled coil1142 – 117433 Potential
Compositional bias267 – 27711Poly-Glu
Compositional bias767 – 7715Poly-Leu

Natural variations

Alternative sequence187 – 19812Missing in isoform 3.
VSP_054225
Alternative sequence505 – 1264760Missing in isoform 2.
VSP_022306
Natural variant3971R → M.
Corresponds to variant rs2446927 [ dbSNP | Ensembl ].
VAR_051797
Natural variant9431H → Y.
Corresponds to variant rs3133711 [ dbSNP | Ensembl ].
VAR_051798

Experimental info

Sequence conflict861Missing in AAH92411. Ref.5
Sequence conflict1311A → P in AAH47060. Ref.5
Sequence conflict1671P → S in AAG49397. Ref.1
Sequence conflict3781E → K in AAH36665. Ref.5
Sequence conflict4231K → R in AAG49397. Ref.1
Sequence conflict6921N → D in AAG49397. Ref.1
Sequence conflict995 – 9973LQK → DAW in CAB55986. Ref.6
Sequence conflict10701S → T in AAG49397. Ref.1
Sequence conflict12331K → KK in CAB55986. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 3.
Checksum: 8647E53FE14B9D5D

FASTA1,264147,163
        10         20         30         40         50         60 
MPEKRLTAEP PTITEEEFED SLATDDFLVD YFNEFLSLPT FSEAIRFNAD YGVFEVANDA 

        70         80         90        100        110        120 
PQFLEKQLKK ILQNQQPRNP IYDVVRKGKN EVKPVQMNAP DEDETINVNY NIMCLSREEG 

       130        140        150        160        170        180 
IKWIKKERLP AFLESDCYFE YRLAKLVSQV RWSKSGMNFT VGSNFSPWIV KKPPSLPPPA 

       190        200        210        220        230        240 
TEEDNLVIMK KFYVSLGEAS YTQTKDWFAL AKQSQQTVST FSLPCCVPYN KLKSPAISSV 

       250        260        270        280        290        300 
SENFIFDDGV HPRTKKDPSK TNKLISEFEE EEGEEEEVSV SLQDTPSQAL LRVYLEKKQD 

       310        320        330        340        350        360 
VDESLTMHFS TCEEFLSSYI YFILRGAIQQ IVGKPVGETP DYINFNNITK VSFDDCFESI 

       370        380        390        400        410        420 
HGKNFLSELV QTTKERSEEI EQTSLSSKNE SAGPESRADW CISHRTYDIG NRKEFERFKK 

       430        440        450        460        470        480 
FIKGTLGERY WWLWMDIERL KVLKDPGRHQ RHLEKMKKCY LVSNGDYYLS AEILSKFKLL 

       490        500        510        520        530        540 
DGSQWNEEHL RNIQSEVLKP LLLYWAPRFC VTHSASTKYA SAELKFWHLR QAKPRKDIDP 

       550        560        570        580        590        600 
FPQMATLLPL RPKSCIPQIP EIQKEEFSLS QPPKSPNKSP EVKTATQKPW KRELLYPGSS 

       610        620        630        640        650        660 
KDDVIEKGSK YMSESSKVIH LTSFTDISEC LKPQLDRRYA YTEEPRVKTV SDVGALGGSD 

       670        680        690        700        710        720 
MENLLQSLYV ENRAGFFFTK FCEHSGNKLW KNSVYFWFDL QAYHQLFYQE TLQPFKVCKQ 

       730        740        750        760        770        780 
AQYLFATYVA PSATLDIGLQ QEKKKEIYMK IQPPFEDLFD TAEEYILLLL LEPWTKMVKS 

       790        800        810        820        830        840 
DQIAYKKVEL VEETRQLDST YFRKLQALHK ETFSKKAEDT TCEIGTGILS LSNVSKRTEY 

       850        860        870        880        890        900 
WDNVPAEYKH FKFSDLLNNK LEFEHFRQFL ETHSSSMDLM CWTDIEQFRR ITYRDRNQRK 

       910        920        930        940        950        960 
AKSIYIKNKY LNKKYFFGPN SPASLYQQNQ VMHLSGGWGK ILHEQLDAPV LVEIQKHVQN 

       970        980        990       1000       1010       1020 
RLENVWLPLF LASEQFAARQ KIKVQMKDIA EELLLQKAEK KIGVWKPVES KWISSSCKII 

      1030       1040       1050       1060       1070       1080 
AFRKALLNPV TSRQFQRFVA LKGDLLENGL LFWQEVQKYK DLCHSHCDES VIQKKITTII 

      1090       1100       1110       1120       1130       1140 
NCFINSSIPP ALQIDIPVEQ AQKIIEHRKE LGPYVFREAQ MTIFGVLFKF WPQFCEFRKN 

      1150       1160       1170       1180       1190       1200 
LTDENIMSVL ERRQEYNKQK KKLAVLEDEK SGKDGIKQYA NTSVPAIKTA LLSDSFLGLQ 

      1210       1220       1230       1240       1250       1260 
PYGRQPTWCY SKYIEALEQE RILLKIQEEL EKKLFAGLQP LTNFKASSST MSLKKNMSAH 


SSQK 

« Hide

Isoform 2 [UniParc].

Checksum: EB2E679C9E730552
Show »

FASTA50458,519
Isoform 3 [UniParc].

Checksum: A7CB6BFD5C6F2E6E
Show »

FASTA1,252145,768

References

« Hide 'large scale' references
[1]"RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits."
Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z., Sha J.
Biol. Reprod. 79:1021-1029(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH GNA11; GNA12 AND GNA13, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 995-1264 (ISOFORM 1).
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY009106 mRNA. Translation: AAG49397.1. Frameshift.
AK292559 mRNA. Translation: BAF85248.1.
AC021590 Genomic DNA. No translation available.
AP005356 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91794.1.
BC036665 mRNA. Translation: AAH36665.2.
BC047060 mRNA. Translation: AAH47060.1.
BC092411 mRNA. Translation: AAH92411.1.
AL117544 mRNA. Translation: CAB55986.2.
PIRT17296.
RefSeqNP_001273621.1. NM_001286692.1.
NP_056483.3. NM_015668.4.
UniGeneHs.120021.

3D structure databases

ProteinModelPortalQ8NE09.
SMRQ8NE09. Positions 845-977, 1021-1136.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000354109.

PTM databases

PhosphoSiteQ8NE09.

Polymorphism databases

DMDM122064954.

Proteomic databases

PaxDbQ8NE09.
PRIDEQ8NE09.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360863; ENSP00000354109; ENSG00000132554. [Q8NE09-1]
ENST00000519725; ENSP00000427798; ENSG00000132554. [Q8NE09-2]
ENST00000523437; ENSP00000428212; ENSG00000132554.
GeneID26166.
KEGGhsa:26166.
UCSCuc003yjb.1. human. [Q8NE09-1]

Organism-specific databases

CTD26166.
GeneCardsGC08M100973.
H-InvDBHIX0019311.
HGNCHGNC:24499. RGS22.
HPAHPA023118.
MIM615650. gene.
neXtProtNX_Q8NE09.
PharmGKBPA142671068.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40652.
HOGENOMHOG000013183.
HOVERGENHBG054528.
InParanoidQ8NE09.
OMAWAPRFCV.
OrthoDBEOG7SN8CM.
PhylomeDBQ8NE09.
TreeFamTF329128.

Gene expression databases

ArrayExpressQ8NE09.
BgeeQ8NE09.
CleanExHS_RGS22.
GenevestigatorQ8NE09.

Family and domain databases

InterProIPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
[Graphical view]
PfamPF00615. RGS. 3 hits.
[Graphical view]
SMARTSM00315. RGS. 2 hits.
[Graphical view]
SUPFAMSSF48097. SSF48097. 4 hits.
PROSITEPS50132. RGS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRGS22. human.
GenomeRNAi26166.
NextBio48271.
PROQ8NE09.
SOURCESearch...

Entry information

Entry nameRGS22_HUMAN
AccessionPrimary (citable) accession number: Q8NE09
Secondary accession number(s): A8K944 expand/collapse secondary AC list , Q569L2, Q86Y71, Q9BYZ4, Q9UFN6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: April 16, 2014
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM