Q8NE01 (CNNM3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal transporter CNNM3

Alternative name(s):
Ancient conserved domain-containing protein 3
Cyclin-M3

Gene names

Name:	CNNM3
Synonyms:	ACDP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable metal transporter By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Expressed at higher level in heart and spleen. Ref.4
Miscellaneous	Shares weak sequence similarity with the cyclin family, hence its name. However, it has no cyclin-like function in vivo.
Sequence similarities	Belongs to the ACDP family. Contains 2 CBS domains. Contains 1 DUF21 domain.
Sequence caution	The sequence AAF86377.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH07199.3 differs from that shown. Reason: Erroneous initiation. The sequence BAA90891.1 differs from that shown. Reason: Frameshift at position 315.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	CBS domain Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MDFI	Q99750	3	EBI-741032,EBI-724076

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NE01-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NE01-2)

The sequence of this isoform differs from the canonical sequence as follows:
     410-457: Missing.

Isoform 3 (identifier: Q8NE01-3)

The sequence of this isoform differs from the canonical sequence as follows:
     410-457: Missing.
     694-707: GVPVEGSPGRNPGV → SLPLLPRGRDSAAYSDSDLFGLSHLVSAVTAFVWP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Metal transporter CNNM3		PRO_0000295763
Regions
	Transmembrane	11 – 27	17	Helical; Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Transmembrane	261 – 281	21	Helical; Potential
	Domain	137 – 288	152	DUF21
	Domain	318 – 379	62	CBS 1
	Domain	386 – 452	67	CBS 2
Amino acid modifications
	Modified residue	661	1	Phosphoserine Ref.7
	Modified residue	700	1	Phosphoserine By similarity
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	410 – 457	48	Missing in isoform 2 and isoform 3.		VSP_027082
	Alternative sequence	694 – 707	14	GVPVE…RNPGV → SLPLLPRGRDSAAYSDSDLF GLSHLVSAVTAFVWP in isoform 3.		VSP_027083
Experimental info
	Sequence conflict	315	1	E → Q in BAA90891. Ref.1
	Sequence conflict	404	1	E → Q in BAA90891. Ref.1
	Sequence conflict	407	1	K → M in BAA90891. Ref.1
	Sequence conflict	482	1	K → T in BAA90891. Ref.1
	Sequence conflict	532	1	Q → H in BAA90891. Ref.1
	Sequence conflict	533	1	E → A in BAA90891. Ref.1
	Sequence conflict	543	1	A → V in BAA90891. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 22BE4A950A8DA7F5	FASTA	707	76,119
	10 20 30 40 50 60 MAAAVAAAGR LGWLFAALCL GNAAGEAAPG PRVLGFCLEE DGAAGAGWVR GGAARDTPDA 70 80 90 100 110 120 TFLLRLFGPG FANSSWSWVA PEGAGCREEA ASPAGEWRAL LRLRLRAEAV RPHSALLAVR 130 140 150 160 170 180 VEPGGGAAEE AAPPWALGLG AAGLLALAAL ARGLQLSALA LAPAEVQVLR ESGSEAERAA 190 200 210 220 230 240 ARRLEPARRW AGCALGALLL LASLAQAALA VLLYRAAGQR AVPAVLGSAG LVFLVGEVVP 250 260 270 280 290 300 AAVSGRWTLA LAPRALGLSR LAVLLTLPVA LPVGQLLELA ARPGRLRERV LELARGGGDP 310 320 330 340 350 360 YSDLSKGVLR CRTVEDVLTP LEDCFMLDAS TVLDFGVLAS IMQSGHTRIP VYEEERSNIV 370 380 390 400 410 420 DMLYLKDLAF VDPEDCTPLS TITRFYNHPL HFVFNDTKLD AVLEEFKRGK SHLAIVQKVN 430 440 450 460 470 480 NEGEGDPFYE VLGLVTLEDV IEEIIRSEIL DESEDYRDTV VKRKPASLMA PLKRKEEFSL 490 500 510 520 530 540 FKVSDDEYKV TISPQLLLAT QRFLSREVDV FSPLRISEKV LLHLLKHPSV NQEVRFDESN 550 560 570 580 590 600 RLATHHYLYQ RSQPVDYFIL ILQGRVEVEI GKEGLKFENG AFTYYGVSAL TVPSSVHQSP 610 620 630 640 650 660 VSSLQPIRHD LQPDPGDGTH SSAYCPDYTV RALSDLQLIK VTRLQYLNAL LATRAQNLPQ 670 680 690 700 SPENTDLQVI PGSQTRLLGE KTTTAAGSSH SRPGVPVEGS PGRNPGV
	Isoform 2 [UniParc]. Checksum: D5D6950DBB25517B	FASTA	659	70,632
	10 20 30 40 50 60 MAAAVAAAGR LGWLFAALCL GNAAGEAAPG PRVLGFCLEE DGAAGAGWVR GGAARDTPDA 70 80 90 100 110 120 TFLLRLFGPG FANSSWSWVA PEGAGCREEA ASPAGEWRAL LRLRLRAEAV RPHSALLAVR 130 140 150 160 170 180 VEPGGGAAEE AAPPWALGLG AAGLLALAAL ARGLQLSALA LAPAEVQVLR ESGSEAERAA 190 200 210 220 230 240 ARRLEPARRW AGCALGALLL LASLAQAALA VLLYRAAGQR AVPAVLGSAG LVFLVGEVVP 250 260 270 280 290 300 AAVSGRWTLA LAPRALGLSR LAVLLTLPVA LPVGQLLELA ARPGRLRERV LELARGGGDP 310 320 330 340 350 360 YSDLSKGVLR CRTVEDVLTP LEDCFMLDAS TVLDFGVLAS IMQSGHTRIP VYEEERSNIV 370 380 390 400 410 420 DMLYLKDLAF VDPEDCTPLS TITRFYNHPL HFVFNDTKLD AVLEEFKRGD TVVKRKPASL 430 440 450 460 470 480 MAPLKRKEEF SLFKVSDDEY KVTISPQLLL ATQRFLSREV DVFSPLRISE KVLLHLLKHP 490 500 510 520 530 540 SVNQEVRFDE SNRLATHHYL YQRSQPVDYF ILILQGRVEV EIGKEGLKFE NGAFTYYGVS 550 560 570 580 590 600 ALTVPSSVHQ SPVSSLQPIR HDLQPDPGDG THSSAYCPDY TVRALSDLQL IKVTRLQYLN 610 620 630 640 650 ALLATRAQNL PQSPENTDLQ VIPGSQTRLL GEKTTTAAGS SHSRPGVPVE GSPGRNPGV
	Isoform 3 [UniParc]. Checksum: 96670B9CE30C8E47	FASTA	680	73,057
	10 20 30 40 50 60 MAAAVAAAGR LGWLFAALCL GNAAGEAAPG PRVLGFCLEE DGAAGAGWVR GGAARDTPDA 70 80 90 100 110 120 TFLLRLFGPG FANSSWSWVA PEGAGCREEA ASPAGEWRAL LRLRLRAEAV RPHSALLAVR 130 140 150 160 170 180 VEPGGGAAEE AAPPWALGLG AAGLLALAAL ARGLQLSALA LAPAEVQVLR ESGSEAERAA 190 200 210 220 230 240 ARRLEPARRW AGCALGALLL LASLAQAALA VLLYRAAGQR AVPAVLGSAG LVFLVGEVVP 250 260 270 280 290 300 AAVSGRWTLA LAPRALGLSR LAVLLTLPVA LPVGQLLELA ARPGRLRERV LELARGGGDP 310 320 330 340 350 360 YSDLSKGVLR CRTVEDVLTP LEDCFMLDAS TVLDFGVLAS IMQSGHTRIP VYEEERSNIV 370 380 390 400 410 420 DMLYLKDLAF VDPEDCTPLS TITRFYNHPL HFVFNDTKLD AVLEEFKRGD TVVKRKPASL 430 440 450 460 470 480 MAPLKRKEEF SLFKVSDDEY KVTISPQLLL ATQRFLSREV DVFSPLRISE KVLLHLLKHP 490 500 510 520 530 540 SVNQEVRFDE SNRLATHHYL YQRSQPVDYF ILILQGRVEV EIGKEGLKFE NGAFTYYGVS 550 560 570 580 590 600 ALTVPSSVHQ SPVSSLQPIR HDLQPDPGDG THSSAYCPDY TVRALSDLQL IKVTRLQYLN 610 620 630 640 650 660 ALLATRAQNL PQSPENTDLQ VIPGSQTRLL GEKTTTAAGS SHSRPSLPLL PRGRDSAAYS 670 680 DSDLFGLSHL VSAVTAFVWP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 313-707 (ISOFORM 2). Tissue: Adipose tissue and Amygdala.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-707 (ISOFORM 3). Tissue: Adrenal cortex, Muscle and Testis.
[4]	"Molecular cloning and characterization of a novel gene family of four ancient conserved domain proteins (ACDP)." Wang C.-Y., Shi J.-D., Yang P., Kumar P.G., Li Q.-Z., Run Q.-G., Su Y.-C., Scott H.S., Kao K.-J., She J.-X. Gene 306:37-44(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 294-707 (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Brain.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-661, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK000025 mRNA. Translation: BAA90891.1. Frameshift. AK126847 mRNA. Translation: BAG54379.1. AC092636 Genomic DNA. Translation: AAY14964.1. BC007199 mRNA. Translation: AAH07199.3. Different initiation. BC022944 mRNA. Translation: AAH22944.1. BC037272 mRNA. Translation: AAH37272.1. AF216965 mRNA. Translation: AAF86377.1. Different initiation.
IPI	IPI00168565. IPI00386115. IPI00641658.
RefSeq	NP_060093.3. NM_017623.4. NP_951060.1. NM_199078.2.
UniGene	Hs.711617.
3D structure databases
ProteinModelPortal	Q8NE01.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NE01. 8 interactions.
MINT	MINT-1452431.
STRING	9606.ENSP00000305449.
PTM databases
PhosphoSite	Q8NE01.
Polymorphism databases
DMDM	74751242.
Proteomic databases
PaxDb	Q8NE01.
PRIDE	Q8NE01.
Protocols and materials databases
DNASU	26505.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305510; ENSP00000305449; ENSG00000168763. ENST00000377060; ENSP00000366260; ENSG00000168763.
GeneID	26505.
KEGG	hsa:26505.
UCSC	uc002swy.3. human. uc002swz.3. human.
Organism-specific databases
CTD	26505.
GeneCards	GC02P097481.
H-InvDB	HIX0002282. HIX0117685.
HGNC	HGNC:104. CNNM3.
HPA	HPA016711.
MIM	607804. gene.
neXtProt	NX_Q8NE01.
PharmGKB	PA26670.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1253.
HOGENOM	HOG000231947.
HOVERGEN	HBG074775.
InParanoid	Q8NE01.
KO	K16302.
OMA	RRWAGCA.
PhylomeDB	Q8NE01.
Gene expression databases
Bgee	Q8NE01.
CleanEx	HS_CNNM3.
Genevestigator	Q8NE01.
Family and domain databases
InterPro	IPR000644. Cysta_beta_synth_core. IPR002550. DUF21. [Graphical view]
Pfam	PF01595. DUF21. 1 hit. [Graphical view]
PROSITE	PS51371. CBS. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CNNM3. human.
GenomeRNAi	26505.
NextBio	48786.
SOURCE	Search...

Entry information

Entry name

CNNM3_HUMAN

Accession

Primary (citable) accession number: Q8NE01
Secondary accession number(s): B3KX67 , Q8TBV4, Q96IW4, Q9NRK4, Q9NXW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.