Q8NE00 (TM104_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protein 104

Gene names

Name:

TMEM104

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the TMEM104 family.
Sequence caution	The sequence BAB84855.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NE00-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NE00-2)

The sequence of this isoform differs from the canonical sequence as follows:
     244-314: AFAVMIVLAL...RLVFLDYVLI → DGPPGSVMGR...LPPHRSTRPT
     315-496: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Transmembrane protein 104		PRO_0000254178
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Potential
	Topological domain	32 – 36	5	Extracellular Potential
	Transmembrane	37 – 57	21	Helical; Potential
	Topological domain	58 – 146	89	Cytoplasmic Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Topological domain	168 – 204	37	Extracellular Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Topological domain	226 – 233	8	Cytoplasmic Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Topological domain	255 – 276	22	Extracellular Potential
	Transmembrane	277 – 297	21	Helical; Potential
	Topological domain	298 – 306	9	Cytoplasmic Potential
	Transmembrane	307 – 327	21	Helical; Potential
	Topological domain	328 – 354	27	Extracellular Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Topological domain	376 – 397	22	Cytoplasmic Potential
	Transmembrane	398 – 418	21	Helical; Potential
	Topological domain	419 – 421	3	Extracellular Potential
	Transmembrane	422 – 442	21	Helical; Potential
	Topological domain	443 – 470	28	Cytoplasmic Potential
	Transmembrane	471 – 491	21	Helical; Potential
	Topological domain	492 – 496	5	Extracellular Potential
Amino acid modifications
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	244 – 314	71	AFAVM…DYVLI → DGPPGSVMGRAEEDASPAWP RSRCRCGGKLKLNSLYPTSA TCMAPDAPGAGQVKVGVNFL CLPPHRSTRPT in isoform 2.		VSP_021199
	Alternative sequence	315 – 496	182	Missing in isoform 2.		VSP_021200
	Natural variant	58	1	V → M. Ref.1 Ref.2 Corresponds to variant rs2016126 [ dbSNP | Ensembl ].		VAR_028831
	Natural variant	439	1	A → T. Corresponds to variant rs3803784 [ dbSNP | Ensembl ].		VAR_028832

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 4173D25DB4870426	FASTA	496	55,679
	10 20 30 40 50 60 MAGEITETGE LYSSYVGLVY MFNLIVGTGA LTMPKAFATA GWLVSLVLLV FLGFMSFVTT 70 80 90 100 110 120 TFVIEAMAAA NAQLHWKRME NLKEEEDDDS STASDSDVLI RDNYERAEKR PILSVQRRGS 130 140 150 160 170 180 PNPFEITDRV EMGQMASMFF NKVGVNLFYF CIIVYLYGDL AIYAAAVPFS LMQVTCSATG 190 200 210 220 230 240 NDSCGVEADT KYNDTDRCWG PLRRVDAYRI YLAIFTLLLG PFTFFDVQKT KYLQILTSLM 250 260 270 280 290 300 RWIAFAVMIV LALIRIGHGQ GEGHPPLADF SGVRNLFGVC VYSFMCQHSL PSLITPVSSK 310 320 330 340 350 360 RHLTRLVFLD YVLILAFYGL LSFTAIFCFR GDSLMDMYTL NFARCDVVGL AAVRFFLGLF 370 380 390 400 410 420 PVFTISTNFP IIAVTLRNNW KTLFHREGGT YPWVVDRVVF PTITLVPPVL VAFCTHDLES 430 440 450 460 470 480 LVGITGAYAG TGIQYVIPAF LVYHCRRDTQ LAFGCGVSNK HRSPFRHTFW VGFVLLWAFS 490 CFIFVTANII LSETKL
	Isoform 2 [UniParc]. Checksum: 405BCEC0DFFCAEE9	FASTA	314	34,796
	10 20 30 40 50 60 MAGEITETGE LYSSYVGLVY MFNLIVGTGA LTMPKAFATA GWLVSLVLLV FLGFMSFVTT 70 80 90 100 110 120 TFVIEAMAAA NAQLHWKRME NLKEEEDDDS STASDSDVLI RDNYERAEKR PILSVQRRGS 130 140 150 160 170 180 PNPFEITDRV EMGQMASMFF NKVGVNLFYF CIIVYLYGDL AIYAAAVPFS LMQVTCSATG 190 200 210 220 230 240 NDSCGVEADT KYNDTDRCWG PLRRVDAYRI YLAIFTLLLG PFTFFDVQKT KYLQILTSLM 250 260 270 280 290 300 RWIDGPPGSV MGRAEEDASP AWPRSRCRCG GKLKLNSLYP TSATCMAPDA PGAGQVKVGV 310 NFLCLPPHRS TRPT

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT MET-58. Tissue: Colon mucosa and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-58. Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 243-496 (ISOFORM 1). Tissue: Testis.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK000262 mRNA. Translation: BAA91039.1. AK074029 mRNA. Translation: BAB84855.1. Different initiation. BC037278 mRNA. Translation: AAH37278.1. AL137521 mRNA. Translation: CAB70787.1.
IPI	IPI00291835. IPI00793242.
PIR	T46367.
RefSeq	NP_060198.3. NM_017728.3.
UniGene	Hs.370262.
3D structure databases
ProteinModelPortal	Q8NE00.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NE00. 1 interaction.
STRING	9606.ENSP00000334849.
Protein family/group databases
TCDB	2.A.18.10.1. amino acid/auxin permease (AAAP) family.
PTM databases
PhosphoSite	Q8NE00.
Polymorphism databases
DMDM	117949798.
Proteomic databases
PaxDb	Q8NE00.
PRIDE	Q8NE00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335464; ENSP00000334849; ENSG00000109066. ENST00000582330; ENSP00000461922; ENSG00000109066. ENST00000582773; ENSP00000463205; ENSG00000109066.
GeneID	54868.
KEGG	hsa:54868.
UCSC	uc002jls.4. human. uc010wrf.1. human.
Organism-specific databases
CTD	54868.
GeneCards	GC17P072772.
HGNC	HGNC:25984. TMEM104.
HPA	HPA044330.
neXtProt	NX_Q8NE00.
PharmGKB	PA142670753.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG242666.
HOGENOM	HOG000231060.
HOVERGEN	HBG080085.
InParanoid	Q8NE00.
OMA	MYTLNFT.
Gene expression databases
ArrayExpress	Q8NE00.
Bgee	Q8NE00.
CleanEx	HS_TMEM104.
Genevestigator	Q8NE00.
GermOnline	ENSG00000109066. Homo sapiens.
Family and domain databases
InterPro	IPR013057. AA_transpt_TM. [Graphical view]
Pfam	PF01490. Aa_trans. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TMEM104. human.
GenomeRNAi	54868.
NextBio	57775.

Entry information

Entry name

TM104_HUMAN

Accession

Primary (citable) accession number: Q8NE00
Secondary accession number(s): Q8TEU1, Q9NT56, Q9NXH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.