Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8NDT2

- RB15B_HUMAN

UniProt

Q8NDT2 - RB15B_HUMAN

Protein

Putative RNA-binding protein 15B

Gene

RBM15B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (05 May 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May function in the regulation of alternative or illicit splicing.1 Publication

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. RNA binding Source: UniProtKB

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. RNA splicing Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW
    6. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, mRNA processing, mRNA splicing, Transcription, Transcription regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative RNA-binding protein 15B
    Alternative name(s):
    One-twenty two protein 3
    Short name:
    HuOTT3
    RNA-binding motif protein 15B
    Gene namesi
    Name:RBM15B
    Synonyms:OTT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:24303. RBM15B.

    Subcellular locationi

    Nucleusnucleoplasm 1 Publication
    Note: Colocalizes with BMLF1 in the nucleus. Localized in the nucleoplasm with a granular staining pattern and excluded from the nucleoli.

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134870079.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 890890Putative RNA-binding protein 15BPRO_0000081778Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei109 – 1091Phosphoserine2 Publications
    Modified residuei267 – 2671Phosphoserine1 Publication
    Modified residuei552 – 5521Phosphoserine2 Publications
    Modified residuei562 – 5621Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8NDT2.
    PaxDbiQ8NDT2.
    PeptideAtlasiQ8NDT2.
    PRIDEiQ8NDT2.

    PTM databases

    PhosphoSiteiQ8NDT2.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    BgeeiQ8NDT2.
    CleanExiHS_RBM15B.
    GenevestigatoriQ8NDT2.

    Organism-specific databases

    HPAiHPA036645.

    Interactioni

    Subunit structurei

    Interacts (via the SPOC domain) with Epstein-Barr virus BMLF1 (via the N-terminus); the interaction is direct. May interact with NCOR2.1 Publication

    Protein-protein interaction databases

    BioGridi118943. 16 interactions.
    IntActiQ8NDT2. 5 interactions.
    STRINGi9606.ENSP00000313890.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NDT2.
    SMRiQ8NDT2. Positions 338-492, 722-866.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini139 – 21981RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini337 – 41478RRM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini418 – 49275RRM 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini711 – 889179SPOCPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni722 – 890169Interaction with Epstein-Barr virus BMLF1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi593 – 5975Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi658 – 67720His-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RRM Spen family.Curated
    Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation
    Contains 1 SPOC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG250829.
    HOGENOMiHOG000006547.
    HOVERGENiHBG058366.
    InParanoidiQ8NDT2.
    KOiK13190.
    OMAiKRDSERN.
    OrthoDBiEOG7QZG9W.
    PhylomeDBiQ8NDT2.
    TreeFamiTF354224.

    Family and domain databases

    Gene3Di2.40.290.10. 1 hit.
    3.30.70.330. 3 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR012921. SPOC_C.
    IPR016194. SPOC_like_C_dom.
    IPR010912. SPOC_met.
    [Graphical view]
    PfamiPF00076. RRM_1. 2 hits.
    PF07744. SPOC. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 3 hits.
    [Graphical view]
    SUPFAMiSSF100939. SSF100939. 1 hit.
    PROSITEiPS50102. RRM. 3 hits.
    PS50917. SPOC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NDT2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKRQSERDSS PSGRGSSSSA KRPREREREA EAGGRRAAHK ASGGAKHPVP    50
    ARARDKPRGS GSGGGGHRDG RGTGDANHRA SSGRSSGSGA GGGGRGGKAS 100
    GDPGASGMSP RASPLPPPPP PPGAEPACPG SSAAAPEYKT LLISSLSPAL 150
    PAEHLEDRLF HQFKRFGEIS LRLSHTPELG RVAYVNFRHP QDAREARQHA 200
    LARQLLLYDR PLKVEPVYLR GGGGSSRRSS SSSAAASTPP PGPPAPADPL 250
    GYLPLHGGYQ YKQRSLSPVA APPLREPRAR HAAAAFALDA AAAAAVGLSR 300
    ERALDYYGLY DDRGRPYGYP AVCEEDLMPE DDQRATRNLF IGNLDHSVSE 350
    VELRRAFEKY GIIEEVVIKR PARGQGGAYA FLKFQNLDMA HRAKVAMSGR 400
    VIGRNPIKIG YGKANPTTRL WVGGLGPNTS LAALAREFDR FGSIRTIDHV 450
    KGDSFAYIQY ESLDAAQAAC AKMRGFPLGG PDRRLRVDFA KAEETRYPQQ 500
    YQPSPLPVHY ELLTDGYTRH RNLDADLVRD RTPPHLLYSD RDRTFLEGDW 550
    TSPSKSSDRR NSLEGYSRSV RSRSGERWGA DGDRGLPKPW EERRKRRSLS 600
    SDRGRTTHSP YEERSRTKGS GQQSERGSDR TPERSRKENH SSEGTKESSS 650
    NSLSNSRHGA EERGHHHHHH EAADSSHGKK ARDSERNHRT TEAEPKPLEE 700
    PKHETKKLKN LSEYAQTLQL GWNGLLVLKN SCFPTSMHIL EGDQGVISSL 750
    LKDHTSGSKL TQLKIAQRLR LDQPKLDEVT RRIKQGSPNG YAVLLATQAT 800
    PSGLGTEGMP TVEPGLQRRL LRNLVSYLKQ KQAAGVISLP VGGSKGRDGT 850
    GMLYAFPPCD FSQQYLQSAL RTLGKLEEEH MVIVIVRDTA 890
    Length:890
    Mass (Da):97,205
    Last modified:May 5, 2009 - v3
    Checksum:i276C94252F036C83
    GO
    Isoform 2 (identifier: Q8NDT2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-327: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:563
    Mass (Da):63,156
    Checksum:i310F8918227B576B
    GO

    Sequence cautioni

    The sequence AAS50153.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part.
    The sequence AAH01367.2 differs from that shown. Reason: Erroneous initiation.
    The sequence CAD38547.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti593 – 5931R → K in AAI39837. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 327327Missing in isoform 2. 1 PublicationVSP_053878Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC092037 Genomic DNA. No translation available.
    BC001367 mRNA. Translation: AAH01367.2. Different initiation.
    BC139836 mRNA. Translation: AAI39837.1.
    AL831838 mRNA. Translation: CAD38547.2. Different initiation.
    AY545557 mRNA. Translation: AAS50153.1. Sequence problems.
    CCDSiCCDS33764.1. [Q8NDT2-1]
    RefSeqiNP_037418.3. NM_013286.4. [Q8NDT2-1]
    UniGeneiHs.118738.
    Hs.716623.
    Hs.731737.

    Genome annotation databases

    EnsembliENST00000563281; ENSP00000454545; ENSG00000259956. [Q8NDT2-1]
    GeneIDi29890.
    KEGGihsa:29890.
    UCSCiuc003dbd.3. human. [Q8NDT2-1]

    Polymorphism databases

    DMDMi229463030.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC092037 Genomic DNA. No translation available.
    BC001367 mRNA. Translation: AAH01367.2 . Different initiation.
    BC139836 mRNA. Translation: AAI39837.1 .
    AL831838 mRNA. Translation: CAD38547.2 . Different initiation.
    AY545557 mRNA. Translation: AAS50153.1 . Sequence problems.
    CCDSi CCDS33764.1. [Q8NDT2-1 ]
    RefSeqi NP_037418.3. NM_013286.4. [Q8NDT2-1 ]
    UniGenei Hs.118738.
    Hs.716623.
    Hs.731737.

    3D structure databases

    ProteinModelPortali Q8NDT2.
    SMRi Q8NDT2. Positions 338-492, 722-866.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118943. 16 interactions.
    IntActi Q8NDT2. 5 interactions.
    STRINGi 9606.ENSP00000313890.

    PTM databases

    PhosphoSitei Q8NDT2.

    Polymorphism databases

    DMDMi 229463030.

    Proteomic databases

    MaxQBi Q8NDT2.
    PaxDbi Q8NDT2.
    PeptideAtlasi Q8NDT2.
    PRIDEi Q8NDT2.

    Protocols and materials databases

    DNASUi 29890.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000563281 ; ENSP00000454545 ; ENSG00000259956 . [Q8NDT2-1 ]
    GeneIDi 29890.
    KEGGi hsa:29890.
    UCSCi uc003dbd.3. human. [Q8NDT2-1 ]

    Organism-specific databases

    CTDi 29890.
    GeneCardsi GC03P051428.
    HGNCi HGNC:24303. RBM15B.
    HPAi HPA036645.
    MIMi 612602. gene.
    neXtProti NX_Q8NDT2.
    PharmGKBi PA134870079.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250829.
    HOGENOMi HOG000006547.
    HOVERGENi HBG058366.
    InParanoidi Q8NDT2.
    KOi K13190.
    OMAi KRDSERN.
    OrthoDBi EOG7QZG9W.
    PhylomeDBi Q8NDT2.
    TreeFami TF354224.

    Miscellaneous databases

    GenomeRNAii 29890.
    NextBioi 35461874.
    PROi Q8NDT2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8NDT2.
    CleanExi HS_RBM15B.
    Genevestigatori Q8NDT2.

    Family and domain databases

    Gene3Di 2.40.290.10. 1 hit.
    3.30.70.330. 3 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR012921. SPOC_C.
    IPR016194. SPOC_like_C_dom.
    IPR010912. SPOC_met.
    [Graphical view ]
    Pfami PF00076. RRM_1. 2 hits.
    PF07744. SPOC. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 3 hits.
    [Graphical view ]
    SUPFAMi SSF100939. SSF100939. 1 hit.
    PROSITEi PS50102. RRM. 3 hits.
    PS50917. SPOC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Colon.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 155-890 (ISOFORM 1).
      Tissue: Brain.
    4. Sales M.M., Ferrasi A.C., Pereira A.A., Pardini M.I.M.C., Camargo A.A.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 633-780.
      Tissue: Head.
    5. "Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export."
      Hiriart E., Gruffat H., Buisson M., Mikaelian I., Keppler S., Meresse P., Mercher T., Bernard O.A., Sergeant A., Manet E.
      J. Biol. Chem. 280:36935-36945(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EPSTEIN-BARR VIRUS BMLF1 AND NCOR2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109; SER-552 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRB15B_HUMAN
    AccessioniPrimary (citable) accession number: Q8NDT2
    Secondary accession number(s): A4QPG7, Q6QE19, Q9BV96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3