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Q8NDT2 (RB15B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative RNA-binding protein 15B
Alternative name(s):
One-twenty two protein 3
Short name=HuOTT3
RNA-binding motif protein 15B
Gene names
Name:RBM15B
Synonyms:OTT3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function in the regulation of alternative or illicit splicing. Ref.5

Subunit structure

Interacts (via the SPOC domain) with Epstein-Barr virus BMLF1 (via the N-terminus); the interaction is direct. May interact with NCOR2. Ref.5

Subcellular location

Nucleusnucleoplasm. Note: Colocalizes with BMLF1 in the nucleus. Localized in the nucleoplasm with a granular staining pattern and excluded from the nucleoli. Ref.5

Tissue specificity

Ubiquitously expressed. Ref.5

Sequence similarities

Belongs to the RRM Spen family.

Contains 3 RRM (RNA recognition motif) domains.

Contains 1 SPOC domain.

Sequence caution

The sequence AAH01367.2 differs from that shown. Reason: Erroneous initiation.

The sequence AAS50153.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part.

The sequence CAD38547.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NDT2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NDT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-327: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Putative RNA-binding protein 15B
PRO_0000081778

Regions

Domain139 – 21981RRM 1
Domain337 – 41478RRM 2
Domain418 – 49275RRM 3
Domain711 – 889179SPOC
Region722 – 890169Interaction with Epstein-Barr virus BMLF1
Motif593 – 5975Nuclear localization signal Potential
Compositional bias658 – 67720His-rich

Amino acid modifications

Modified residue1091Phosphoserine Ref.11 Ref.12
Modified residue2671Phosphoserine Ref.10
Modified residue5521Phosphoserine Ref.11 Ref.12
Modified residue5621Phosphoserine Ref.12

Natural variations

Alternative sequence1 – 327327Missing in isoform 2.
VSP_053878

Experimental info

Sequence conflict5931R → K in AAI39837. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: 276C94252F036C83

FASTA89097,205
        10         20         30         40         50         60 
MKRQSERDSS PSGRGSSSSA KRPREREREA EAGGRRAAHK ASGGAKHPVP ARARDKPRGS 

        70         80         90        100        110        120 
GSGGGGHRDG RGTGDANHRA SSGRSSGSGA GGGGRGGKAS GDPGASGMSP RASPLPPPPP 

       130        140        150        160        170        180 
PPGAEPACPG SSAAAPEYKT LLISSLSPAL PAEHLEDRLF HQFKRFGEIS LRLSHTPELG 

       190        200        210        220        230        240 
RVAYVNFRHP QDAREARQHA LARQLLLYDR PLKVEPVYLR GGGGSSRRSS SSSAAASTPP 

       250        260        270        280        290        300 
PGPPAPADPL GYLPLHGGYQ YKQRSLSPVA APPLREPRAR HAAAAFALDA AAAAAVGLSR 

       310        320        330        340        350        360 
ERALDYYGLY DDRGRPYGYP AVCEEDLMPE DDQRATRNLF IGNLDHSVSE VELRRAFEKY 

       370        380        390        400        410        420 
GIIEEVVIKR PARGQGGAYA FLKFQNLDMA HRAKVAMSGR VIGRNPIKIG YGKANPTTRL 

       430        440        450        460        470        480 
WVGGLGPNTS LAALAREFDR FGSIRTIDHV KGDSFAYIQY ESLDAAQAAC AKMRGFPLGG 

       490        500        510        520        530        540 
PDRRLRVDFA KAEETRYPQQ YQPSPLPVHY ELLTDGYTRH RNLDADLVRD RTPPHLLYSD 

       550        560        570        580        590        600 
RDRTFLEGDW TSPSKSSDRR NSLEGYSRSV RSRSGERWGA DGDRGLPKPW EERRKRRSLS 

       610        620        630        640        650        660 
SDRGRTTHSP YEERSRTKGS GQQSERGSDR TPERSRKENH SSEGTKESSS NSLSNSRHGA 

       670        680        690        700        710        720 
EERGHHHHHH EAADSSHGKK ARDSERNHRT TEAEPKPLEE PKHETKKLKN LSEYAQTLQL 

       730        740        750        760        770        780 
GWNGLLVLKN SCFPTSMHIL EGDQGVISSL LKDHTSGSKL TQLKIAQRLR LDQPKLDEVT 

       790        800        810        820        830        840 
RRIKQGSPNG YAVLLATQAT PSGLGTEGMP TVEPGLQRRL LRNLVSYLKQ KQAAGVISLP 

       850        860        870        880        890 
VGGSKGRDGT GMLYAFPPCD FSQQYLQSAL RTLGKLEEEH MVIVIVRDTA 

« Hide

Isoform 2 [UniParc].

Checksum: 310F8918227B576B
Show »

FASTA56363,156

References

« Hide 'large scale' references
[1]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Colon.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 155-890 (ISOFORM 1).
Tissue: Brain.
[4]Sales M.M., Ferrasi A.C., Pereira A.A., Pardini M.I.M.C., Camargo A.A.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 633-780.
Tissue: Head.
[5]"Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export."
Hiriart E., Gruffat H., Buisson M., Mikaelian I., Keppler S., Meresse P., Mercher T., Bernard O.A., Sergeant A., Manet E.
J. Biol. Chem. 280:36935-36945(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EPSTEIN-BARR VIRUS BMLF1 AND NCOR2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109; SER-552 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC092037 Genomic DNA. No translation available.
BC001367 mRNA. Translation: AAH01367.2. Different initiation.
BC139836 mRNA. Translation: AAI39837.1.
AL831838 mRNA. Translation: CAD38547.2. Different initiation.
AY545557 mRNA. Translation: AAS50153.1. Sequence problems.
CCDSCCDS33764.1.
RefSeqNP_037418.3. NM_013286.4. [Q8NDT2-1]
UniGeneHs.118738.
Hs.716623.
Hs.731737.

3D structure databases

ProteinModelPortalQ8NDT2.
SMRQ8NDT2. Positions 338-492, 722-866.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118943. 15 interactions.
IntActQ8NDT2. 5 interactions.
STRING9606.ENSP00000313890.

PTM databases

PhosphoSiteQ8NDT2.

Polymorphism databases

DMDM229463030.

Proteomic databases

MaxQBQ8NDT2.
PaxDbQ8NDT2.
PeptideAtlasQ8NDT2.
PRIDEQ8NDT2.

Protocols and materials databases

DNASU29890.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000323686; ENSP00000313890; ENSG00000179837.
ENST00000563281; ENSP00000454545; ENSG00000259956.
GeneID29890.
KEGGhsa:29890.
UCSCuc003dbd.3. human. [Q8NDT2-1]

Organism-specific databases

CTD29890.
GeneCardsGC03P051428.
HGNCHGNC:24303. RBM15B.
HPAHPA036645.
MIM612602. gene.
neXtProtNX_Q8NDT2.
PharmGKBPA134870079.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250829.
HOGENOMHOG000006547.
HOVERGENHBG058366.
InParanoidQ8NDT2.
KOK13190.
OMAKRDSERN.
OrthoDBEOG7QZG9W.
PhylomeDBQ8NDT2.
TreeFamTF354224.

Gene expression databases

BgeeQ8NDT2.
CleanExHS_RBM15B.
GenevestigatorQ8NDT2.

Family and domain databases

Gene3D2.40.290.10. 1 hit.
3.30.70.330. 3 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012921. SPOC_C.
IPR016194. SPOC_like_C_dom.
IPR010912. SPOC_met.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
PF07744. SPOC. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMSSF100939. SSF100939. 1 hit.
PROSITEPS50102. RRM. 3 hits.
PS50917. SPOC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi29890.
NextBio35461874.
PROQ8NDT2.
SOURCESearch...

Entry information

Entry nameRB15B_HUMAN
AccessionPrimary (citable) accession number: Q8NDT2
Secondary accession number(s): A4QPG7, Q6QE19, Q9BV96
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: May 5, 2009
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM