Q8NDL9 (CBPC5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic carboxypeptidase-like protein 5
EC=3.4.17.-

Alternative name(s):
ATP/GTP-binding protein-like 5

Gene names

Name:	AGBL5
Synonyms:	CCP5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	886 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6 By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity. Note: Mainly cytoplasmic. Slight accumulation in the nucleus is observed By similarity.
Tissue specificity	Expressed in brain.
Sequence similarities	Belongs to the peptidase M14 family.
Sequence caution	The sequence AAX93164.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAY14655.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB15151.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein branching point deglutamylation Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metallocarboxypeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NDL9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NDL9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     786-816: ARPRLGRGSPPTRRGMKGSSGPTSPTPRTRE → TCPRRVSARRGPGFPRLGPGWAGAHRRLAEG
     817-886: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NDL9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     697-717: EPRSQDRRRQQQPLNHRPAGS → GKPVWEPLQHVFGCLGHCWGK
     718-886: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 886	886	Cytosolic carboxypeptidase-like protein 5		PRO_0000305921
Regions
	Compositional bias	540 – 544	5	Poly-Pro
	Compositional bias	651 – 654	4	Poly-Ser
Sites
	Active site	303	1	Nucleophile By similarity
	Metal binding	252	1	Zinc By similarity
	Metal binding	255	1	Zinc By similarity
	Metal binding	434	1	Zinc By similarity
Natural variations
	Alternative sequence	697 – 717	21	EPRSQ…RPAGS → GKPVWEPLQHVFGCLGHCWG K in isoform 3.		VSP_028359
	Alternative sequence	718 – 886	169	Missing in isoform 3.		VSP_028360
	Alternative sequence	786 – 816	31	ARPRL…PRTRE → TCPRRVSARRGPGFPRLGPG WAGAHRRLAEG in isoform 2.		VSP_028361
	Alternative sequence	817 – 886	70	Missing in isoform 2.		VSP_028362
	Natural variant	649	1	G → D. Corresponds to variant rs35804461 [ dbSNP | Ensembl ].		VAR_035231
Experimental info
	Sequence conflict	38 – 40	3	GAS → CLL in AAI31499. Ref.5
	Sequence conflict	94	1	N → S in BAH13623. Ref.2
	Sequence conflict	122	1	I → R in AAI31499. Ref.5
	Sequence conflict	672	1	S → G in BAB15151. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0948AD0F37B0725A	FASTA	886	97,534
	10 20 30 40 50 60 MELRCGGLLF SSRFDSGNLA HVEKVESLSS DGEGVGGGAS ALTSGIASSP DYEFNVWTRP 70 80 90 100 110 120 DCAETEFENG NRSWFYFSVR GGMPGKLIKI NIMNMNKQSK LYSQGMAPFV RTLPTRPRWE 130 140 150 160 170 180 RIRDRPTFEM TETQFVLSFV HRFVEGRGAT TFFAFCYPFS YSDCQELLNQ LDQRFPENHP 190 200 210 220 230 240 THSSPLDTIY YHRELLCYSL DGLRVDLLTI TSCHGLREDR EPRLEQLFPD TSTPRPFRFA 250 260 270 280 290 300 GKRIFFLSSR VHPGETPSSF VFNGFLDFIL RPDDPRAQTL RRLFVFKLIP MLNPDGVVRG 310 320 330 340 350 360 HYRTDSRGVN LNRQYLKPDA VLHPAIYGAK AVLLYHHVHS RLNSQSSSEH QPSSCLPPDA 370 380 390 400 410 420 PVSDLEKANN LQNEAQCGHS ADRHNAEAWK QTEPAEQKLN SVWIMPQQSA GLEESAPDTI 430 440 450 460 470 480 PPKESGVAYY VDLHGHASKR GCFMYGNSFS DESTQVENML YPKLISLNSA HFDFQGCNFS 490 500 510 520 530 540 EKNMYARDRR DGQSKEGSGR VAIYKASGII HSYTLECNYN TGRSVNSIPA ACHDNGRASP 550 560 570 580 590 600 PPPPAFPSRY TVELFEQVGR AMAIAALDMA ECNPWPRIVL SEHSSLTNLR AWMLKHVRNS 610 620 630 640 650 660 RGLSSTLNVG VNKKRGLRTP PKSHNGLPVS CSENTLSRAR SFSTGTSAGG SSSSQQNSPQ 670 680 690 700 710 720 MKNSPSFPFH GSRPAGLPGL GSSTQKVTHR VLGPVREPRS QDRRRQQQPL NHRPAGSLAP 730 740 750 760 770 780 SPAPTSSGPA SSHKLGSCLL PDSFNIPGSS CSLLSSGDKP EAVMVIGKGL LGTGARMPCI 790 800 810 820 830 840 KTRLQARPRL GRGSPPTRRG MKGSSGPTSP TPRTRESSEL ELGSCSATPG LPQARPPRPR 850 860 870 880 SAPAFSPISC SLSDSPSWNC YSRGPLGQPE VCFVPKSPPL TVSPRV
	Isoform 2 [UniParc]. Checksum: FD717AAC3A12A1BE	FASTA	816	90,252
	10 20 30 40 50 60 MELRCGGLLF SSRFDSGNLA HVEKVESLSS DGEGVGGGAS ALTSGIASSP DYEFNVWTRP 70 80 90 100 110 120 DCAETEFENG NRSWFYFSVR GGMPGKLIKI NIMNMNKQSK LYSQGMAPFV RTLPTRPRWE 130 140 150 160 170 180 RIRDRPTFEM TETQFVLSFV HRFVEGRGAT TFFAFCYPFS YSDCQELLNQ LDQRFPENHP 190 200 210 220 230 240 THSSPLDTIY YHRELLCYSL DGLRVDLLTI TSCHGLREDR EPRLEQLFPD TSTPRPFRFA 250 260 270 280 290 300 GKRIFFLSSR VHPGETPSSF VFNGFLDFIL RPDDPRAQTL RRLFVFKLIP MLNPDGVVRG 310 320 330 340 350 360 HYRTDSRGVN LNRQYLKPDA VLHPAIYGAK AVLLYHHVHS RLNSQSSSEH QPSSCLPPDA 370 380 390 400 410 420 PVSDLEKANN LQNEAQCGHS ADRHNAEAWK QTEPAEQKLN SVWIMPQQSA GLEESAPDTI 430 440 450 460 470 480 PPKESGVAYY VDLHGHASKR GCFMYGNSFS DESTQVENML YPKLISLNSA HFDFQGCNFS 490 500 510 520 530 540 EKNMYARDRR DGQSKEGSGR VAIYKASGII HSYTLECNYN TGRSVNSIPA ACHDNGRASP 550 560 570 580 590 600 PPPPAFPSRY TVELFEQVGR AMAIAALDMA ECNPWPRIVL SEHSSLTNLR AWMLKHVRNS 610 620 630 640 650 660 RGLSSTLNVG VNKKRGLRTP PKSHNGLPVS CSENTLSRAR SFSTGTSAGG SSSSQQNSPQ 670 680 690 700 710 720 MKNSPSFPFH GSRPAGLPGL GSSTQKVTHR VLGPVREPRS QDRRRQQQPL NHRPAGSLAP 730 740 750 760 770 780 SPAPTSSGPA SSHKLGSCLL PDSFNIPGSS CSLLSSGDKP EAVMVIGKGL LGTGARMPCI 790 800 810 KTRLQTCPRR VSARRGPGFP RLGPGWAGAH RRLAEG
	Isoform 3 [UniParc]. Checksum: 67418CCF89A6734E	FASTA	717	80,016
	10 20 30 40 50 60 MELRCGGLLF SSRFDSGNLA HVEKVESLSS DGEGVGGGAS ALTSGIASSP DYEFNVWTRP 70 80 90 100 110 120 DCAETEFENG NRSWFYFSVR GGMPGKLIKI NIMNMNKQSK LYSQGMAPFV RTLPTRPRWE 130 140 150 160 170 180 RIRDRPTFEM TETQFVLSFV HRFVEGRGAT TFFAFCYPFS YSDCQELLNQ LDQRFPENHP 190 200 210 220 230 240 THSSPLDTIY YHRELLCYSL DGLRVDLLTI TSCHGLREDR EPRLEQLFPD TSTPRPFRFA 250 260 270 280 290 300 GKRIFFLSSR VHPGETPSSF VFNGFLDFIL RPDDPRAQTL RRLFVFKLIP MLNPDGVVRG 310 320 330 340 350 360 HYRTDSRGVN LNRQYLKPDA VLHPAIYGAK AVLLYHHVHS RLNSQSSSEH QPSSCLPPDA 370 380 390 400 410 420 PVSDLEKANN LQNEAQCGHS ADRHNAEAWK QTEPAEQKLN SVWIMPQQSA GLEESAPDTI 430 440 450 460 470 480 PPKESGVAYY VDLHGHASKR GCFMYGNSFS DESTQVENML YPKLISLNSA HFDFQGCNFS 490 500 510 520 530 540 EKNMYARDRR DGQSKEGSGR VAIYKASGII HSYTLECNYN TGRSVNSIPA ACHDNGRASP 550 560 570 580 590 600 PPPPAFPSRY TVELFEQVGR AMAIAALDMA ECNPWPRIVL SEHSSLTNLR AWMLKHVRNS 610 620 630 640 650 660 RGLSSTLNVG VNKKRGLRTP PKSHNGLPVS CSENTLSRAR SFSTGTSAGG SSSSQQNSPQ 670 680 690 700 710 MKNSPSFPFH GSRPAGLPGL GSSTQKVTHR VLGPVRGKPV WEPLQHVFGC LGHCWGK

References

[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Ovary and Testis.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL833844 mRNA. Translation: CAD38704.1. AK302091 mRNA. Translation: BAH13623.1. AC013403 Genomic DNA. Translation: AAX93164.1. Sequence problems. AC013472 Genomic DNA. Translation: AAY14655.1. Sequence problems. CH471053 Genomic DNA. Translation: EAX00649.1. CH471053 Genomic DNA. Translation: EAX00651.1. CH471053 Genomic DNA. Translation: EAX00652.1. CH471053 Genomic DNA. Translation: EAX00653.1. BC007415 mRNA. Translation: AAH07415.2. BC018584 mRNA. No translation available. BC131498 mRNA. Translation: AAI31499.1. AK023398 mRNA. Translation: BAB14560.1. AK025492 mRNA. Translation: BAB15151.1. Different initiation.
IPI	IPI00171550. IPI00186972. IPI00480100.
RefSeq	NP_001030584.1. NM_001035507.2. NP_068603.4. NM_021831.5.
UniGene	Hs.138207.
3D structure databases
ProteinModelPortal	Q8NDL9.
ModBase	Search...
Protein family/group databases
MEROPS	M14.025.
PTM databases
PhosphoSite	Q8NDL9.
Polymorphism databases
DMDM	74715354.
Proteomic databases
PaxDb	Q8NDL9.
PRIDE	Q8NDL9.
Protocols and materials databases
DNASU	60509.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323064; ENSP00000323681; ENSG00000084693. ENST00000360131; ENSP00000353249; ENSG00000084693. ENST00000487078; ENSP00000433830; ENSG00000084693.
GeneID	60509.
KEGG	hsa:60509.
UCSC	uc002rid.3. human. uc002rie.3. human.
Organism-specific databases
CTD	60509.
GeneCards	GC02P027265.
HGNC	HGNC:26147. AGBL5.
HPA	HPA030981.
neXtProt	NX_Q8NDL9.
PharmGKB	PA162375816.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2866.
HOVERGEN	HBG068187.
InParanoid	Q8NDL9.
OMA	DTIPPKE.
OrthoDB	EOG46T30Z.
Gene expression databases
ArrayExpress	Q8NDL9.
Bgee	Q8NDL9.
CleanEx	HS_AGBL5.
Genevestigator	Q8NDL9.
Family and domain databases
InterPro	IPR000834. Peptidase_M14. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. [Graphical view]
PROSITE	PS00132. CARBOXYPEPT_ZN_1. False negative. PS00133. CARBOXYPEPT_ZN_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AGBL5. human.
GenomeRNAi	60509.
NextBio	65419.

Entry information

Entry name

CBPC5_HUMAN

Accession

Primary (citable) accession number: Q8NDL9
Secondary accession number(s): A2VDI7 , B7WPG9, B7Z7I7, D6W548, Q53SW0, Q53SZ0, Q96IK8, Q9H6V0, Q9H8P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.