Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8NDG6

- TDRD9_HUMAN

UniProt

Q8NDG6 - TDRD9_HUMAN

Protein

Putative ATP-dependent RNA helicase TDRD9

Gene

TDRD9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (02 Mar 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi155 – 1628ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. DNA methylation involved in gamete generation Source: UniProtKB
    3. fertilization Source: UniProtKB
    4. gene silencing by RNA Source: UniProtKB-KW
    5. male meiosis Source: UniProtKB
    6. multicellular organismal development Source: UniProtKB-KW
    7. piRNA metabolic process Source: UniProtKB
    8. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Helicase, Hydrolase

    Keywords - Biological processi

    Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ATP-dependent RNA helicase TDRD9 (EC:3.6.4.13)
    Alternative name(s):
    Tudor domain-containing protein 9
    Gene namesi
    Name:TDRD9
    Synonyms:C14orf75
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:20122. TDRD9.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies By similarity.By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. piP-body Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134890186.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13821382Putative ATP-dependent RNA helicase TDRD9PRO_0000333813Add
    BLAST

    Proteomic databases

    PaxDbiQ8NDG6.
    PRIDEiQ8NDG6.

    PTM databases

    PhosphoSiteiQ8NDG6.

    Expressioni

    Gene expression databases

    BgeeiQ8NDG6.
    CleanExiHS_TDRD9.
    GenevestigatoriQ8NDG6.

    Organism-specific databases

    HPAiHPA038120.

    Interactioni

    Subunit structurei

    Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.By similarity

    Protein-protein interaction databases

    BioGridi125766. 1 interaction.
    STRINGi9606.ENSP00000387303.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NDG6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini142 – 308167Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini377 – 544168Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini944 – 100461TudorPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi254 – 2574DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 Tudor domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    InParanoidiQ8NDG6.
    OMAiINDWFTI.
    OrthoDBiEOG7FV3PF.
    PhylomeDBiQ8NDG6.
    TreeFamiTF324869.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR002999. Tudor.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00567. TUDOR. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00333. TUDOR. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50304. TUDOR. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NDG6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLRKLTIEQI NDWFTIGKTV TNVELLGAPP AFPAGAAREE VQRQDVAPGA     50
    GPAAQAPALA QAPARPAAAF ERSLSQRSSE VEYINKYRQL EAQELDVCRS 100
    VQPTSGPGPR PSLAKLSSVT CIPGTTYKYP DLPISRYKEE VVSLIESNSV 150
    VIIHGATGSG KSTQLPQYIL DHYVQRSAYC SIVVTQPRKI GASSIARWIS 200
    KERAWTLGGV VGYQVGLEKI ATEDTRLIYM TTGVLLQKIV SAKSLMEFTH 250
    IIIDEVHERT EEMDFLLLVV RKLLRTNSRF VKVVLMSATI SCKEFADYFA 300
    VPVQNKMNPA YIFEVEGKPH SVEEYYLNDL EHIHHSKLSP HLLEEPVITK 350
    DIYEVAVSLI QMFDDLDMKE SGNKAWSGAQ FVLERSSVLV FLPGLGEINY 400
    MHELLTSLVH KRLQVYPLHS SVALEEQNNV FLSPVPGYRK IILSTNIAES 450
    SVTVPDVKYV IDFCLTRTLV CDEDTNYQSL RLSWASKTSC NQRKGRAGRV 500
    SRGYCYRLVH KDFWDNSIPD HVVPEMLRCP LGSTILKVKL LDMGEPRALL 550
    ATALSPPGLS DIERTILLLK EVGALAVSGQ REDENPHDGE LTFLGRVLAQ 600
    LPVNQQLGKL IVLGHVFGCL DECLIIAAAL SLKNFFAMPF RQHLDGYRNK 650
    VNFSGSSKSD CIALVEAFKT WKACRQTGEL RYPKDELNWG RLNYIQIKRI 700
    REVAELYEEL KTRISQFNMH VDSRRPVMDQ EYIYKQRFIL QVVLAGAFYP 750
    NYFTFGQPDE EMAVRELAGK DPKTTVVLKH IPPYGFLYYK QLQSLFRQCG 800
    QVKSIVFDGA KAFVEFSRNP TERFKTLPAV YMAIKMSQLK VSLELSVHSA 850
    EEIEGKVQGM NVSKLRNTRV NVDFQKQTVD PMQVSFNTSD RSQTVTDLLL 900
    TIDVTEVVEV GHFWGYRIDE NNSEILKKLT AEINQLTLVP LPTHPHPDLV 950
    CLAPFADFDK QRYFRAQVLY VSGNSAEVFF VDYGNKSHVD LHLLMEIPCQ 1000
    FLELPFQALE FKICKMRPSA KSLVCGKHWS DGASQWFASL VSGCTLLVKV 1050
    FSVVHSVLHV DVYQYSGVQD AINIRDVLIQ QGYAELTEES YESKQSHEVL 1100
    KGLFSKSVEN MTDGSVPFPM KDDEKYLIRI LLESFSTNKL GTPNCKAELH 1150
    GPFNPYELKC HSLTRISKFR CVWIEKESIN SVIISDAPED LHQRMLVAAS 1200
    LSINATGSTM LLRETSLMPH IPGLPALLSM LFAPVIELRI DQNGKYYTGV 1250
    LCGLGWNPAT GASILPEHDM ELAFDVQFSV EDVVEVNILR AAINKLVCDG 1300
    PNGCKCLGPE RVAQLQDIAR QKLLGLFCQS KPREKIVPKW HEKPYEWNQV 1350
    DPKLVMEQAD RESSRGKNTF LYQLHKLVVL GT 1382
    Length:1,382
    Mass (Da):155,683
    Last modified:March 2, 2010 - v3
    Checksum:i8A48E71E4A12041F
    GO
    Isoform 2 (identifier: Q8NDG6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1095-1285: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,191
    Mass (Da):134,466
    Checksum:i6362D3EA9EAC4D2D
    GO

    Sequence cautioni

    The sequence AAI28058.1 differs from that shown. Reason: Frameshift at position 1159.
    The sequence AAI28058.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAC04182.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC05047.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC05144.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC86372.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti758 – 7581P → L in BAC05047. (PubMed:14702039)Curated
    Sequence conflicti1260 – 12601T → G in AAH16796. (PubMed:15489334)Curated
    Sequence conflicti1276 – 12761V → F in BAC05144. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1095 – 1285191Missing in isoform 2. 1 PublicationVSP_033552Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL132712 Genomic DNA. No translation available.
    AL136001 Genomic DNA. No translation available.
    AL833915 mRNA. Translation: CAD38771.1.
    AK093483 mRNA. Translation: BAC04182.1. Different initiation.
    AK097429 mRNA. Translation: BAC05047.1. Different initiation.
    AK097699 mRNA. Translation: BAC05144.1. Different initiation.
    AK125978 mRNA. Translation: BAC86372.1. Different initiation.
    BC016796 mRNA. Translation: AAH16796.1.
    BC128057 mRNA. Translation: AAI28058.1. Sequence problems.
    CCDSiCCDS9987.2. [Q8NDG6-1]
    PIRiG02632.
    RefSeqiNP_694591.2. NM_153046.2. [Q8NDG6-1]
    UniGeneiHs.21454.

    Genome annotation databases

    EnsembliENST00000339063; ENSP00000343545; ENSG00000156414. [Q8NDG6-2]
    ENST00000409874; ENSP00000387303; ENSG00000156414. [Q8NDG6-1]
    GeneIDi122402.
    KEGGihsa:122402.
    UCSCiuc001yom.4. human. [Q8NDG6-1]
    uc001yon.4. human. [Q8NDG6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL132712 Genomic DNA. No translation available.
    AL136001 Genomic DNA. No translation available.
    AL833915 mRNA. Translation: CAD38771.1 .
    AK093483 mRNA. Translation: BAC04182.1 . Different initiation.
    AK097429 mRNA. Translation: BAC05047.1 . Different initiation.
    AK097699 mRNA. Translation: BAC05144.1 . Different initiation.
    AK125978 mRNA. Translation: BAC86372.1 . Different initiation.
    BC016796 mRNA. Translation: AAH16796.1 .
    BC128057 mRNA. Translation: AAI28058.1 . Sequence problems.
    CCDSi CCDS9987.2. [Q8NDG6-1 ]
    PIRi G02632.
    RefSeqi NP_694591.2. NM_153046.2. [Q8NDG6-1 ]
    UniGenei Hs.21454.

    3D structure databases

    ProteinModelPortali Q8NDG6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125766. 1 interaction.
    STRINGi 9606.ENSP00000387303.

    PTM databases

    PhosphoSitei Q8NDG6.

    Proteomic databases

    PaxDbi Q8NDG6.
    PRIDEi Q8NDG6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000339063 ; ENSP00000343545 ; ENSG00000156414 . [Q8NDG6-2 ]
    ENST00000409874 ; ENSP00000387303 ; ENSG00000156414 . [Q8NDG6-1 ]
    GeneIDi 122402.
    KEGGi hsa:122402.
    UCSCi uc001yom.4. human. [Q8NDG6-1 ]
    uc001yon.4. human. [Q8NDG6-2 ]

    Organism-specific databases

    CTDi 122402.
    GeneCardsi GC14P104394.
    HGNCi HGNC:20122. TDRD9.
    HPAi HPA038120.
    neXtProti NX_Q8NDG6.
    PharmGKBi PA134890186.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1643.
    InParanoidi Q8NDG6.
    OMAi INDWFTI.
    OrthoDBi EOG7FV3PF.
    PhylomeDBi Q8NDG6.
    TreeFami TF324869.

    Miscellaneous databases

    GenomeRNAii 122402.
    NextBioi 80877.
    PROi Q8NDG6.

    Gene expression databases

    Bgeei Q8NDG6.
    CleanExi HS_TDRD9.
    Genevestigatori Q8NDG6.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR002999. Tudor.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00567. TUDOR. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00333. TUDOR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50304. TUDOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-1382 (ISOFORM 1).
      Tissue: Testis.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-1382 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 635-1382 (ISOFORM 1).
      Tissue: Testis.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-1382 (ISOFORM 1).
      Tissue: Bone marrow.

    Entry informationi

    Entry nameiTDRD9_HUMAN
    AccessioniPrimary (citable) accession number: Q8NDG6
    Secondary accession number(s): A1A4S7
    , Q6ZU54, Q8N7T3, Q8N827, Q8N9V5, Q96AS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3