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Q8NDG6 (TDRD9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative ATP-dependent RNA helicase TDRD9

EC=3.6.4.13
Alternative name(s):
Tudor domain-containing protein 9
Gene names
Name:TDRD9
Synonyms:C14orf75
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1382 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with piRNA-associated proteins PIWIL1 and PIWIL4 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies By similarity.

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 Tudor domain.

Sequence caution

The sequence AAI28058.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI28058.1 differs from that shown. Reason: Frameshift at position 1159.

The sequence BAC04182.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC05047.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC05144.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC86372.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDifferentiation
Meiosis
RNA-mediated gene silencing
Spermatogenesis
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Helicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA methylation involved in gamete generation

Inferred from sequence or structural similarity. Source: UniProtKB

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

fertilization

Inferred from sequence or structural similarity. Source: UniProtKB

gene silencing by RNA

Inferred from electronic annotation. Source: UniProtKB-KW

male meiosis

Inferred from sequence or structural similarity. Source: UniProtKB

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

piRNA metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

piP-body

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NDG6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NDG6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1095-1285: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13821382Putative ATP-dependent RNA helicase TDRD9
PRO_0000333813

Regions

Domain142 – 308167Helicase ATP-binding
Domain377 – 544168Helicase C-terminal
Domain944 – 100461Tudor
Nucleotide binding155 – 1628ATP By similarity
Motif254 – 2574DEAH box

Natural variations

Alternative sequence1095 – 1285191Missing in isoform 2.
VSP_033552

Experimental info

Sequence conflict7581P → L in BAC05047. Ref.3
Sequence conflict12601T → G in AAH16796. Ref.4
Sequence conflict12761V → F in BAC05144. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 2, 2010. Version 3.
Checksum: 8A48E71E4A12041F

FASTA1,382155,683
        10         20         30         40         50         60 
MLRKLTIEQI NDWFTIGKTV TNVELLGAPP AFPAGAAREE VQRQDVAPGA GPAAQAPALA 

        70         80         90        100        110        120 
QAPARPAAAF ERSLSQRSSE VEYINKYRQL EAQELDVCRS VQPTSGPGPR PSLAKLSSVT 

       130        140        150        160        170        180 
CIPGTTYKYP DLPISRYKEE VVSLIESNSV VIIHGATGSG KSTQLPQYIL DHYVQRSAYC 

       190        200        210        220        230        240 
SIVVTQPRKI GASSIARWIS KERAWTLGGV VGYQVGLEKI ATEDTRLIYM TTGVLLQKIV 

       250        260        270        280        290        300 
SAKSLMEFTH IIIDEVHERT EEMDFLLLVV RKLLRTNSRF VKVVLMSATI SCKEFADYFA 

       310        320        330        340        350        360 
VPVQNKMNPA YIFEVEGKPH SVEEYYLNDL EHIHHSKLSP HLLEEPVITK DIYEVAVSLI 

       370        380        390        400        410        420 
QMFDDLDMKE SGNKAWSGAQ FVLERSSVLV FLPGLGEINY MHELLTSLVH KRLQVYPLHS 

       430        440        450        460        470        480 
SVALEEQNNV FLSPVPGYRK IILSTNIAES SVTVPDVKYV IDFCLTRTLV CDEDTNYQSL 

       490        500        510        520        530        540 
RLSWASKTSC NQRKGRAGRV SRGYCYRLVH KDFWDNSIPD HVVPEMLRCP LGSTILKVKL 

       550        560        570        580        590        600 
LDMGEPRALL ATALSPPGLS DIERTILLLK EVGALAVSGQ REDENPHDGE LTFLGRVLAQ 

       610        620        630        640        650        660 
LPVNQQLGKL IVLGHVFGCL DECLIIAAAL SLKNFFAMPF RQHLDGYRNK VNFSGSSKSD 

       670        680        690        700        710        720 
CIALVEAFKT WKACRQTGEL RYPKDELNWG RLNYIQIKRI REVAELYEEL KTRISQFNMH 

       730        740        750        760        770        780 
VDSRRPVMDQ EYIYKQRFIL QVVLAGAFYP NYFTFGQPDE EMAVRELAGK DPKTTVVLKH 

       790        800        810        820        830        840 
IPPYGFLYYK QLQSLFRQCG QVKSIVFDGA KAFVEFSRNP TERFKTLPAV YMAIKMSQLK 

       850        860        870        880        890        900 
VSLELSVHSA EEIEGKVQGM NVSKLRNTRV NVDFQKQTVD PMQVSFNTSD RSQTVTDLLL 

       910        920        930        940        950        960 
TIDVTEVVEV GHFWGYRIDE NNSEILKKLT AEINQLTLVP LPTHPHPDLV CLAPFADFDK 

       970        980        990       1000       1010       1020 
QRYFRAQVLY VSGNSAEVFF VDYGNKSHVD LHLLMEIPCQ FLELPFQALE FKICKMRPSA 

      1030       1040       1050       1060       1070       1080 
KSLVCGKHWS DGASQWFASL VSGCTLLVKV FSVVHSVLHV DVYQYSGVQD AINIRDVLIQ 

      1090       1100       1110       1120       1130       1140 
QGYAELTEES YESKQSHEVL KGLFSKSVEN MTDGSVPFPM KDDEKYLIRI LLESFSTNKL 

      1150       1160       1170       1180       1190       1200 
GTPNCKAELH GPFNPYELKC HSLTRISKFR CVWIEKESIN SVIISDAPED LHQRMLVAAS 

      1210       1220       1230       1240       1250       1260 
LSINATGSTM LLRETSLMPH IPGLPALLSM LFAPVIELRI DQNGKYYTGV LCGLGWNPAT 

      1270       1280       1290       1300       1310       1320 
GASILPEHDM ELAFDVQFSV EDVVEVNILR AAINKLVCDG PNGCKCLGPE RVAQLQDIAR 

      1330       1340       1350       1360       1370       1380 
QKLLGLFCQS KPREKIVPKW HEKPYEWNQV DPKLVMEQAD RESSRGKNTF LYQLHKLVVL 


GT 

« Hide

Isoform 2 [UniParc].

Checksum: 6362D3EA9EAC4D2D
Show »

FASTA1,191134,466

References

[1]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-1382 (ISOFORM 1).
Tissue: Testis.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-1382 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 635-1382 (ISOFORM 1).
Tissue: Testis.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-1382 (ISOFORM 1).
Tissue: Bone marrow.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL132712 Genomic DNA. No translation available.
AL136001 Genomic DNA. No translation available.
AL833915 mRNA. Translation: CAD38771.1.
AK093483 mRNA. Translation: BAC04182.1. Different initiation.
AK097429 mRNA. Translation: BAC05047.1. Different initiation.
AK097699 mRNA. Translation: BAC05144.1. Different initiation.
AK125978 mRNA. Translation: BAC86372.1. Different initiation.
BC016796 mRNA. Translation: AAH16796.1.
BC128057 mRNA. Translation: AAI28058.1. Sequence problems.
PIRG02632.
RefSeqNP_694591.2. NM_153046.2.
UniGeneHs.21454.

3D structure databases

ProteinModelPortalQ8NDG6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125766. 1 interaction.
STRING9606.ENSP00000387303.

PTM databases

PhosphoSiteQ8NDG6.

Proteomic databases

PaxDbQ8NDG6.
PRIDEQ8NDG6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339063; ENSP00000343545; ENSG00000156414. [Q8NDG6-2]
ENST00000409874; ENSP00000387303; ENSG00000156414. [Q8NDG6-1]
GeneID122402.
KEGGhsa:122402.
UCSCuc001yom.4. human. [Q8NDG6-1]
uc001yon.4. human. [Q8NDG6-2]

Organism-specific databases

CTD122402.
GeneCardsGC14P104394.
HGNCHGNC:20122. TDRD9.
HPAHPA038120.
neXtProtNX_Q8NDG6.
PharmGKBPA134890186.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1643.
InParanoidQ8NDG6.
OMAINDWFTI.
OrthoDBEOG7FV3PF.
PhylomeDBQ8NDG6.
TreeFamTF324869.

Gene expression databases

BgeeQ8NDG6.
CleanExHS_TDRD9.
GenevestigatorQ8NDG6.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi122402.
NextBio80877.
PROQ8NDG6.

Entry information

Entry nameTDRD9_HUMAN
AccessionPrimary (citable) accession number: Q8NDG6
Secondary accession number(s): A1A4S7 expand/collapse secondary AC list , Q6ZU54, Q8N7T3, Q8N827, Q8N9V5, Q96AS9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 2, 2010
Last modified: April 16, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM