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Protein

Putative ATP-dependent RNA helicase TDRD9

Gene

TDRD9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi155 – 1628ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_267790. PIWI-interacting RNA (piRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase TDRD9 (EC:3.6.4.13)
Alternative name(s):
Tudor domain-containing protein 9
Gene namesi
Name:TDRD9
Synonyms:C14orf75
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20122. TDRD9.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134890186.

Polymorphism and mutation databases

BioMutaiTDRD9.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13821382Putative ATP-dependent RNA helicase TDRD9PRO_0000333813Add
BLAST

Proteomic databases

PaxDbiQ8NDG6.
PRIDEiQ8NDG6.

PTM databases

PhosphoSiteiQ8NDG6.

Expressioni

Gene expression databases

BgeeiQ8NDG6.
CleanExiHS_TDRD9.
ExpressionAtlasiQ8NDG6. baseline and differential.
GenevisibleiQ8NDG6. HS.

Organism-specific databases

HPAiHPA038120.

Interactioni

Subunit structurei

Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.By similarity

Protein-protein interaction databases

BioGridi125766. 1 interaction.
STRINGi9606.ENSP00000387303.

Structurei

3D structure databases

ProteinModelPortaliQ8NDG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 308167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini377 – 544168Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini944 – 100461TudorPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi254 – 2574DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 Tudor domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000119189.
InParanoidiQ8NDG6.
KOiK18408.
OMAiINDWFTI.
OrthoDBiEOG7FV3PF.
PhylomeDBiQ8NDG6.
TreeFamiTF324869.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NDG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRKLTIEQI NDWFTIGKTV TNVELLGAPP AFPAGAAREE VQRQDVAPGA
60 70 80 90 100
GPAAQAPALA QAPARPAAAF ERSLSQRSSE VEYINKYRQL EAQELDVCRS
110 120 130 140 150
VQPTSGPGPR PSLAKLSSVT CIPGTTYKYP DLPISRYKEE VVSLIESNSV
160 170 180 190 200
VIIHGATGSG KSTQLPQYIL DHYVQRSAYC SIVVTQPRKI GASSIARWIS
210 220 230 240 250
KERAWTLGGV VGYQVGLEKI ATEDTRLIYM TTGVLLQKIV SAKSLMEFTH
260 270 280 290 300
IIIDEVHERT EEMDFLLLVV RKLLRTNSRF VKVVLMSATI SCKEFADYFA
310 320 330 340 350
VPVQNKMNPA YIFEVEGKPH SVEEYYLNDL EHIHHSKLSP HLLEEPVITK
360 370 380 390 400
DIYEVAVSLI QMFDDLDMKE SGNKAWSGAQ FVLERSSVLV FLPGLGEINY
410 420 430 440 450
MHELLTSLVH KRLQVYPLHS SVALEEQNNV FLSPVPGYRK IILSTNIAES
460 470 480 490 500
SVTVPDVKYV IDFCLTRTLV CDEDTNYQSL RLSWASKTSC NQRKGRAGRV
510 520 530 540 550
SRGYCYRLVH KDFWDNSIPD HVVPEMLRCP LGSTILKVKL LDMGEPRALL
560 570 580 590 600
ATALSPPGLS DIERTILLLK EVGALAVSGQ REDENPHDGE LTFLGRVLAQ
610 620 630 640 650
LPVNQQLGKL IVLGHVFGCL DECLIIAAAL SLKNFFAMPF RQHLDGYRNK
660 670 680 690 700
VNFSGSSKSD CIALVEAFKT WKACRQTGEL RYPKDELNWG RLNYIQIKRI
710 720 730 740 750
REVAELYEEL KTRISQFNMH VDSRRPVMDQ EYIYKQRFIL QVVLAGAFYP
760 770 780 790 800
NYFTFGQPDE EMAVRELAGK DPKTTVVLKH IPPYGFLYYK QLQSLFRQCG
810 820 830 840 850
QVKSIVFDGA KAFVEFSRNP TERFKTLPAV YMAIKMSQLK VSLELSVHSA
860 870 880 890 900
EEIEGKVQGM NVSKLRNTRV NVDFQKQTVD PMQVSFNTSD RSQTVTDLLL
910 920 930 940 950
TIDVTEVVEV GHFWGYRIDE NNSEILKKLT AEINQLTLVP LPTHPHPDLV
960 970 980 990 1000
CLAPFADFDK QRYFRAQVLY VSGNSAEVFF VDYGNKSHVD LHLLMEIPCQ
1010 1020 1030 1040 1050
FLELPFQALE FKICKMRPSA KSLVCGKHWS DGASQWFASL VSGCTLLVKV
1060 1070 1080 1090 1100
FSVVHSVLHV DVYQYSGVQD AINIRDVLIQ QGYAELTEES YESKQSHEVL
1110 1120 1130 1140 1150
KGLFSKSVEN MTDGSVPFPM KDDEKYLIRI LLESFSTNKL GTPNCKAELH
1160 1170 1180 1190 1200
GPFNPYELKC HSLTRISKFR CVWIEKESIN SVIISDAPED LHQRMLVAAS
1210 1220 1230 1240 1250
LSINATGSTM LLRETSLMPH IPGLPALLSM LFAPVIELRI DQNGKYYTGV
1260 1270 1280 1290 1300
LCGLGWNPAT GASILPEHDM ELAFDVQFSV EDVVEVNILR AAINKLVCDG
1310 1320 1330 1340 1350
PNGCKCLGPE RVAQLQDIAR QKLLGLFCQS KPREKIVPKW HEKPYEWNQV
1360 1370 1380
DPKLVMEQAD RESSRGKNTF LYQLHKLVVL GT
Length:1,382
Mass (Da):155,683
Last modified:March 2, 2010 - v3
Checksum:i8A48E71E4A12041F
GO
Isoform 2 (identifier: Q8NDG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1095-1285: Missing.

Note: No experimental confirmation available.
Show »
Length:1,191
Mass (Da):134,466
Checksum:i6362D3EA9EAC4D2D
GO

Sequence cautioni

The sequence AAI28058.1 differs from that shown. Reason: Frameshift at position 1159. Curated
The sequence AAI28058.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC04182.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC05047.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC05144.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC86372.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti758 – 7581P → L in BAC05047 (PubMed:14702039).Curated
Sequence conflicti1260 – 12601T → G in AAH16796 (PubMed:15489334).Curated
Sequence conflicti1276 – 12761V → F in BAC05144 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1095 – 1285191Missing in isoform 2. 1 PublicationVSP_033552Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132712 Genomic DNA. No translation available.
AL136001 Genomic DNA. No translation available.
AL833915 mRNA. Translation: CAD38771.1.
AK093483 mRNA. Translation: BAC04182.1. Different initiation.
AK097429 mRNA. Translation: BAC05047.1. Different initiation.
AK097699 mRNA. Translation: BAC05144.1. Different initiation.
AK125978 mRNA. Translation: BAC86372.1. Different initiation.
BC016796 mRNA. Translation: AAH16796.1.
BC128057 mRNA. Translation: AAI28058.1. Sequence problems.
CCDSiCCDS9987.2. [Q8NDG6-1]
PIRiG02632.
RefSeqiNP_694591.2. NM_153046.2. [Q8NDG6-1]
UniGeneiHs.21454.

Genome annotation databases

EnsembliENST00000409874; ENSP00000387303; ENSG00000156414.
GeneIDi122402.
KEGGihsa:122402.
UCSCiuc001yom.4. human. [Q8NDG6-1]
uc001yon.4. human. [Q8NDG6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132712 Genomic DNA. No translation available.
AL136001 Genomic DNA. No translation available.
AL833915 mRNA. Translation: CAD38771.1.
AK093483 mRNA. Translation: BAC04182.1. Different initiation.
AK097429 mRNA. Translation: BAC05047.1. Different initiation.
AK097699 mRNA. Translation: BAC05144.1. Different initiation.
AK125978 mRNA. Translation: BAC86372.1. Different initiation.
BC016796 mRNA. Translation: AAH16796.1.
BC128057 mRNA. Translation: AAI28058.1. Sequence problems.
CCDSiCCDS9987.2. [Q8NDG6-1]
PIRiG02632.
RefSeqiNP_694591.2. NM_153046.2. [Q8NDG6-1]
UniGeneiHs.21454.

3D structure databases

ProteinModelPortaliQ8NDG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125766. 1 interaction.
STRINGi9606.ENSP00000387303.

PTM databases

PhosphoSiteiQ8NDG6.

Polymorphism and mutation databases

BioMutaiTDRD9.

Proteomic databases

PaxDbiQ8NDG6.
PRIDEiQ8NDG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409874; ENSP00000387303; ENSG00000156414.
GeneIDi122402.
KEGGihsa:122402.
UCSCiuc001yom.4. human. [Q8NDG6-1]
uc001yon.4. human. [Q8NDG6-2]

Organism-specific databases

CTDi122402.
GeneCardsiGC14P104394.
HGNCiHGNC:20122. TDRD9.
HPAiHPA038120.
neXtProtiNX_Q8NDG6.
PharmGKBiPA134890186.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000119189.
InParanoidiQ8NDG6.
KOiK18408.
OMAiINDWFTI.
OrthoDBiEOG7FV3PF.
PhylomeDBiQ8NDG6.
TreeFamiTF324869.

Enzyme and pathway databases

ReactomeiREACT_267790. PIWI-interacting RNA (piRNA) biogenesis.

Miscellaneous databases

GenomeRNAii122402.
NextBioi80877.
PROiQ8NDG6.

Gene expression databases

BgeeiQ8NDG6.
CleanExiHS_TDRD9.
ExpressionAtlasiQ8NDG6. baseline and differential.
GenevisibleiQ8NDG6. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-1382 (ISOFORM 1).
    Tissue: Testis.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-1382 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 635-1382 (ISOFORM 1).
    Tissue: Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-1382 (ISOFORM 1).
    Tissue: Bone marrow.

Entry informationi

Entry nameiTDRD9_HUMAN
AccessioniPrimary (citable) accession number: Q8NDG6
Secondary accession number(s): A1A4S7
, Q6ZU54, Q8N7T3, Q8N827, Q8N9V5, Q96AS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 2, 2010
Last modified: July 22, 2015
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.