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Q8NCW5 (NNRE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD(P)H-hydrate epimerase

EC=5.1.99.6
Alternative name(s):
Apolipoprotein A-I-binding protein
Short name=AI-BP
NAD(P)HX epimerase
YjeF N-terminal domain-containing protein 1
Short name=YjeF_N1
Gene names
Name:APOA1BP
Synonyms:AIBP, YJEFN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX By similarity. HAMAP-Rule MF_03159

Catalytic activity

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide. HAMAP-Rule MF_03159

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP-Rule MF_03159

Subunit structure

Homodimer By similarity. Interacts with APOA1 and APOA2. Ref.1

Subcellular location

Mitochondrion By similarity. Secreted. Note: In sperm, secretion gradually increases during capacitation By similarity. Ref.1

Tissue specificity

Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level). Ref.1

Post-translational modification

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation By similarity. HAMAP-Rule MF_03159

Sequence similarities

Belongs to the NnrE/AIBP family.

Contains 1 YjeF N-terminal domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NCW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NCW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4747Mitochondrion Potential
Chain48 – 288241NAD(P)H-hydrate epimerase HAMAP-Rule MF_03159
PRO_5000067606

Regions

Domain65 – 275211YjeF N-terminal
Region119 – 1235NAD(P)HX By similarity
Region189 – 1957NAD(P)HX By similarity

Sites

Metal binding1201Potassium By similarity
Metal binding1851Potassium By similarity
Metal binding2211Potassium By similarity
Binding site2181NAD(P)HX By similarity

Amino acid modifications

Modified residue491Phosphoserine By similarity
Modified residue1441N6-succinyllysine By similarity

Natural variations

Alternative sequence1 – 103103Missing in isoform 2.
VSP_026417
Natural variant191V → L. Ref.1 Ref.4
Corresponds to variant rs7516274 [ dbSNP | Ensembl ].
VAR_032992

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: 1D8B04FEC595EA01

FASTA28831,675
        10         20         30         40         50         60 
MSRLRALLGL GLLVAGSRVP RIKSQTIACR SGPTWWGPQR LNSGGRWDSE VMASTVVKYL 

        70         80         90        100        110        120 
SQEEAQAVDQ ELFNEYQFSV DQLMELAGLS CATAIAKAYP PTSMSRSPPT VLVICGPGNN 

       130        140        150        160        170        180 
GGDGLVCARH LKLFGYEPTI YYPKRPNKPL FTALVTQCQK MDIPFLGEMP AEPMTIDELY 

       190        200        210        220        230        240 
ELVVDAIFGF SFKGDVREPF HSILSVLKGL TVPIASIDIP SGWDVEKGNA GGIQPDLLIS 

       250        260        270        280 
LTAPKKSATQ FTGRYHYLGG RFVPPALEKK YQLNLPPYPD TECVYRLQ 

« Hide

Isoform 2 [UniParc].

Checksum: B0E4FEB583B72308
Show »

FASTA18520,431

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel apolipoprotein A-I-binding protein, AI-BP, secreted by cells of the kidney proximal tubules in response to HDL or ApoA-I."
Ritter M., Buechler C., Boettcher A., Barlage S., Schmitz-Madry A., Orso E., Bared S.M., Schmiedeknecht G., Baehr C.H., Fricker G., Schmitz G.
Genomics 79:693-702(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-19, TISSUE SPECIFICITY, INTERACTION WITH APOA1 AND APOA2, SUBCELLULAR LOCATION.
Tissue: Liver.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT LEU-19.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ315849 mRNA. Translation: CAC86580.1.
AK294835 mRNA. Translation: BAG57944.1.
AL365181 Genomic DNA. Translation: CAI13051.1.
BC100931 mRNA. Translation: AAI00932.1.
BC100932 mRNA. Translation: AAI00933.1.
BC100933 mRNA. Translation: AAI00934.1.
BC100934 mRNA. Translation: AAI00935.1.
RefSeqNP_658985.2. NM_144772.2.
UniGeneHs.528320.

3D structure databases

ProteinModelPortalQ8NCW5.
SMRQ8NCW5. Positions 57-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126103. 5 interactions.
IntActQ8NCW5. 2 interactions.
STRING9606.ENSP00000357218.

PTM databases

PhosphoSiteQ8NCW5.

Polymorphism databases

DMDM150438841.

2D gel databases

REPRODUCTION-2DPAGEIPI00168479.

Proteomic databases

PaxDbQ8NCW5.
PeptideAtlasQ8NCW5.
PRIDEQ8NCW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368235; ENSP00000357218; ENSG00000163382. [Q8NCW5-1]
GeneID128240.
KEGGhsa:128240.
UCSCuc001fph.3. human. [Q8NCW5-1]

Organism-specific databases

CTD128240.
GeneCardsGC01P156561.
HGNCHGNC:18453. APOA1BP.
HPAHPA043766.
HPA048164.
MIM608862. gene.
neXtProtNX_Q8NCW5.
PharmGKBPA38538.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0062.
HOGENOMHOG000174236.
HOVERGENHBG058276.
InParanoidQ8NCW5.
KOK17759.
OMAELMSTGA.
PhylomeDBQ8NCW5.
TreeFamTF300197.

Enzyme and pathway databases

BioCycMetaCyc:ENSG00000163382-MONOMER.

Gene expression databases

ArrayExpressQ8NCW5.
BgeeQ8NCW5.
CleanExHS_APOA1BP.
GenevestigatorQ8NCW5.

Family and domain databases

Gene3D3.40.50.10260. 1 hit.
HAMAPMF_01966. NADHX_epimerase.
InterProIPR026600. NnrE/AIBP.
IPR004443. YjeF_N_dom.
[Graphical view]
PfamPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMSSF64153. SSF64153. 1 hit.
TIGRFAMsTIGR00197. yjeF_nterm. 1 hit.
PROSITEPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAPOA1BP. human.
GenomeRNAi128240.
NextBio82258.
PROQ8NCW5.
SOURCESearch...

Entry information

Entry nameNNRE_HUMAN
AccessionPrimary (citable) accession number: Q8NCW5
Secondary accession number(s): B4DGY3, Q496C6, Q5T3I2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM