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Protein

NAD(P)H-hydrate epimerase

Gene

APOA1BP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K(+)UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201PotassiumUniRule annotation
Metal bindingi185 – 1851PotassiumUniRule annotation
Binding sitei218 – 2181NAD(P)HXUniRule annotation
Metal bindingi221 – 2211PotassiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. NADHX epimerase activity Source: GO_Central
  3. NADPHX epimerase activity Source: GO_Central
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. NADH metabolic process Source: GO_Central
  2. NADP metabolic process Source: GO_Central
  3. protein homotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000163382-MONOMER.
BRENDAi5.1.99.6. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseUniRule annotation
YjeF N-terminal domain-containing protein 1
Short name:
YjeF_N1
Gene namesi
Name:APOA1BPUniRule annotation
Synonyms:AIBPUniRule annotation, YJEFN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:18453. APOA1BP.

Subcellular locationi

Mitochondrion UniRule annotation. Secreted UniRule annotation1 Publication
Note: In sperm, secretion gradually increases during capacitation.UniRule annotation

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. cilium Source: Ensembl
  3. cytoplasm Source: HPA
  4. extracellular region Source: UniProtKB
  5. extracellular space Source: BHF-UCL
  6. extracellular vesicular exosome Source: UniProtKB
  7. intracellular membrane-bounded organelle Source: HPA
  8. mitochondrion Source: GO_Central
  9. nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38538.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4747MitochondrionUniRule annotationAdd
BLAST
Chaini48 – 288241NAD(P)H-hydrate epimerasePRO_5000067606Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei144 – 1441N6-succinyllysineBy similarity

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8NCW5.
PaxDbiQ8NCW5.
PeptideAtlasiQ8NCW5.
PRIDEiQ8NCW5.

2D gel databases

REPRODUCTION-2DPAGEIPI00168479.

PTM databases

PhosphoSiteiQ8NCW5.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level).1 Publication

Gene expression databases

BgeeiQ8NCW5.
CleanExiHS_APOA1BP.
ExpressionAtlasiQ8NCW5. baseline and differential.
GenevestigatoriQ8NCW5.

Organism-specific databases

HPAiHPA043766.
HPA048164.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with APOA1 and APOA2.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi126103. 4 interactions.
IntActiQ8NCW5. 2 interactions.
STRINGi9606.ENSP00000357218.

Structurei

3D structure databases

ProteinModelPortaliQ8NCW5.
SMRiQ8NCW5. Positions 57-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 275211YjeF N-terminalUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1235NAD(P)HXUniRule annotation
Regioni189 – 1957NAD(P)HXUniRule annotation

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0062.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ8NCW5.
KOiK17759.
OMAiGRYHFLG.
PhylomeDBiQ8NCW5.
TreeFamiTF300197.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
[Graphical view]
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NCW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRLRALLGL GLLVAGSRVP RIKSQTIACR SGPTWWGPQR LNSGGRWDSE
60 70 80 90 100
VMASTVVKYL SQEEAQAVDQ ELFNEYQFSV DQLMELAGLS CATAIAKAYP
110 120 130 140 150
PTSMSRSPPT VLVICGPGNN GGDGLVCARH LKLFGYEPTI YYPKRPNKPL
160 170 180 190 200
FTALVTQCQK MDIPFLGEMP AEPMTIDELY ELVVDAIFGF SFKGDVREPF
210 220 230 240 250
HSILSVLKGL TVPIASIDIP SGWDVEKGNA GGIQPDLLIS LTAPKKSATQ
260 270 280
FTGRYHYLGG RFVPPALEKK YQLNLPPYPD TECVYRLQ
Length:288
Mass (Da):31,675
Last modified:June 25, 2007 - v2
Checksum:i1D8B04FEC595EA01
GO
Isoform 2 (identifier: Q8NCW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Show »
Length:185
Mass (Da):20,431
Checksum:iB0E4FEB583B72308
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191V → L.2 Publications
Corresponds to variant rs7516274 [ dbSNP | Ensembl ].
VAR_032992

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform 2. 2 PublicationsVSP_026417Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315849 mRNA. Translation: CAC86580.1.
AK294835 mRNA. Translation: BAG57944.1.
AL365181 Genomic DNA. Translation: CAI13051.1.
BC100931 mRNA. Translation: AAI00932.1.
BC100932 mRNA. Translation: AAI00933.1.
BC100933 mRNA. Translation: AAI00934.1.
BC100934 mRNA. Translation: AAI00935.1.
CCDSiCCDS1145.1. [Q8NCW5-1]
RefSeqiNP_658985.2. NM_144772.2. [Q8NCW5-1]
UniGeneiHs.528320.

Genome annotation databases

EnsembliENST00000368235; ENSP00000357218; ENSG00000163382. [Q8NCW5-1]
GeneIDi128240.
KEGGihsa:128240.
UCSCiuc001fph.3. human. [Q8NCW5-1]

Polymorphism databases

DMDMi150438841.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315849 mRNA. Translation: CAC86580.1.
AK294835 mRNA. Translation: BAG57944.1.
AL365181 Genomic DNA. Translation: CAI13051.1.
BC100931 mRNA. Translation: AAI00932.1.
BC100932 mRNA. Translation: AAI00933.1.
BC100933 mRNA. Translation: AAI00934.1.
BC100934 mRNA. Translation: AAI00935.1.
CCDSiCCDS1145.1. [Q8NCW5-1]
RefSeqiNP_658985.2. NM_144772.2. [Q8NCW5-1]
UniGeneiHs.528320.

3D structure databases

ProteinModelPortaliQ8NCW5.
SMRiQ8NCW5. Positions 57-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126103. 4 interactions.
IntActiQ8NCW5. 2 interactions.
STRINGi9606.ENSP00000357218.

PTM databases

PhosphoSiteiQ8NCW5.

Polymorphism databases

DMDMi150438841.

2D gel databases

REPRODUCTION-2DPAGEIPI00168479.

Proteomic databases

MaxQBiQ8NCW5.
PaxDbiQ8NCW5.
PeptideAtlasiQ8NCW5.
PRIDEiQ8NCW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368235; ENSP00000357218; ENSG00000163382. [Q8NCW5-1]
GeneIDi128240.
KEGGihsa:128240.
UCSCiuc001fph.3. human. [Q8NCW5-1]

Organism-specific databases

CTDi128240.
GeneCardsiGC01P156561.
HGNCiHGNC:18453. APOA1BP.
HPAiHPA043766.
HPA048164.
MIMi608862. gene.
neXtProtiNX_Q8NCW5.
PharmGKBiPA38538.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0062.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ8NCW5.
KOiK17759.
OMAiGRYHFLG.
PhylomeDBiQ8NCW5.
TreeFamiTF300197.

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000163382-MONOMER.
BRENDAi5.1.99.6. 2681.

Miscellaneous databases

ChiTaRSiAPOA1BP. human.
GenomeRNAii128240.
NextBioi82258.
PROiQ8NCW5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8NCW5.
CleanExiHS_APOA1BP.
ExpressionAtlasiQ8NCW5. baseline and differential.
GenevestigatoriQ8NCW5.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
[Graphical view]
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel apolipoprotein A-I-binding protein, AI-BP, secreted by cells of the kidney proximal tubules in response to HDL or ApoA-I."
    Ritter M., Buechler C., Boettcher A., Barlage S., Schmitz-Madry A., Orso E., Bared S.M., Schmiedeknecht G., Baehr C.H., Fricker G., Schmitz G.
    Genomics 79:693-702(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-19, TISSUE SPECIFICITY, INTERACTION WITH APOA1 AND APOA2, SUBCELLULAR LOCATION.
    Tissue: Liver.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT LEU-19.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNNRE_HUMAN
AccessioniPrimary (citable) accession number: Q8NCW5
Secondary accession number(s): B4DGY3, Q496C6, Q5T3I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 25, 2007
Last sequence update: June 25, 2007
Last modified: March 31, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.