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Q8NCL4

- GALT6_HUMAN

UniProt

Q8NCL4 - GALT6_HUMAN

Protein

Polypeptide N-acetylgalactosaminyltransferase 6

Gene

GALNT6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei217 – 2171SubstrateBy similarity
    Binding sitei246 – 2461SubstrateBy similarity
    Metal bindingi269 – 2691ManganeseBy similarity
    Metal bindingi271 – 2711ManganeseBy similarity
    Binding sitei379 – 3791SubstrateBy similarity
    Metal bindingi407 – 4071ManganeseBy similarity
    Binding sitei410 – 4101SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: ProtInc

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. O-glycan processing Source: Reactome
    3. post-translational protein modification Source: Reactome
    4. protein O-linked glycosylation Source: ProtInc

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    BRENDAi2.4.1.41. 2681.
    ReactomeiREACT_115606. O-linked glycosylation of mucins.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 6 (EC:2.4.1.41)
    Alternative name(s):
    Polypeptide GalNAc transferase 6
    Short name:
    GalNAc-T6
    Short name:
    pp-GaNTase 6
    Protein-UDP acetylgalactosaminyltransferase 6
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
    Gene namesi
    Name:GALNT6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:4128. GALNT6.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: BHF-UCL
    2. Golgi membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB-KW
    4. perinuclear region of cytoplasm Source: BHF-UCL

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28541.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Polypeptide N-acetylgalactosaminyltransferase 6PRO_0000059114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi165 ↔ 402PROSITE-ProRule annotation
    Disulfide bondi393 ↔ 474PROSITE-ProRule annotation
    Glycosylationi476 – 4761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi509 ↔ 527PROSITE-ProRule annotation
    Disulfide bondi553 ↔ 566PROSITE-ProRule annotation
    Disulfide bondi597 ↔ 610PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ8NCL4.
    PaxDbiQ8NCL4.
    PRIDEiQ8NCL4.

    PTM databases

    PhosphoSiteiQ8NCL4.

    Expressioni

    Tissue specificityi

    Expressed in placenta and trachea. Weakly expressed in brain and pancreas. Expressed in fibroblast. Weakly or not expressed in lung, liver, muscle, kidney, spleen, thymus, prostate, testis, ovary, intestine, colon, leukocyte, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland and bone marrow.1 Publication

    Gene expression databases

    ArrayExpressiQ8NCL4.
    BgeeiQ8NCL4.
    CleanExiHS_GALNT6.
    GenevestigatoriQ8NCL4.

    Organism-specific databases

    HPAiHPA011762.
    HPA017086.

    Interactioni

    Protein-protein interaction databases

    BioGridi116393. 4 interactions.
    IntActiQ8NCL4. 4 interactions.
    MINTiMINT-5006466.
    STRINGi9606.ENSP00000348668.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NCL4.
    SMRiQ8NCL4. Positions 142-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini29 – 622594LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2820Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini496 – 622127Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 285110Catalytic subdomain AAdd
    BLAST
    Regioni348 – 41063Catalytic subdomain BAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239675.
    HOGENOMiHOG000038227.
    HOVERGENiHBG051699.
    InParanoidiQ8NCL4.
    KOiK00710.
    OMAiNQCLDVG.
    OrthoDBiEOG7J9VP2.
    PhylomeDBiQ8NCL4.
    TreeFamiTF313267.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NCL4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLLRRRHMP LRLAMVGCAF VLFLFLLHRD VSSREEATEK PWLKSLVSRK    50
    DHVLDLMLEA MNNLRDSMPK LQIRAPEAQQ TLFSINQSCL PGFYTPAELK 100
    PFWERPPQDP NAPGADGKAF QKSKWTPLET QEKEEGYKKH CFNAFASDRI 150
    SLQRSLGPDT RPPECVDQKF RRCPPLATTS VIIVFHNEAW STLLRTVYSV 200
    LHTTPAILLK EIILVDDAST EEHLKEKLEQ YVKQLQVVRV VRQEERKGLI 250
    TARLLGASVA QAEVLTFLDA HCECFHGWLE PLLARIAEDK TVVVSPDIVT 300
    IDLNTFEFAK PVQRGRVHSR GNFDWSLTFG WETLPPHEKQ RRKDETYPIK 350
    SPTFAGGLFS ISKSYFEHIG TYDNQMEIWG GENVEMSFRV WQCGGQLEII 400
    PCSVVGHVFR TKSPHTFPKG TSVIARNQVR LAEVWMDSYK KIFYRRNLQA 450
    AKMAQEKSFG DISERLQLRE QLHCHNFSWY LHNVYPEMFV PDLTPTFYGA 500
    IKNLGTNQCL DVGENNRGGK PLIMYSCHGL GGNQYFEYTT QRDLRHNIAK 550
    QLCLHVSKGA LGLGSCHFTG KNSQVPKDEE WELAQDQLIR NSGSGTCLTS 600
    QDKKPAMAPC NPSDPHQLWL FV 622
    Length:622
    Mass (Da):71,159
    Last modified:August 16, 2004 - v2
    Checksum:i38E63FF2AFB486EF
    GO

    Sequence cautioni

    The sequence BAB15297.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti362 – 3621S → P in CAA69876. (PubMed:10464263)Curated
    Sequence conflicti454 – 4541A → T in BAC11118. (PubMed:14702039)Curated
    Sequence conflicti551 – 5511Q → R in BAC11118. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti423 – 4231V → I.
    Corresponds to variant rs747300 [ dbSNP | Ensembl ].
    VAR_019580

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08565 mRNA. Translation: CAA69876.1.
    AK025961 mRNA. Translation: BAB15297.1. Different initiation.
    AK074658 mRNA. Translation: BAC11118.1.
    BC035822 mRNA. Translation: AAH35822.2.
    CCDSiCCDS8813.1.
    RefSeqiNP_009141.2. NM_007210.3.
    XP_005268664.1. XM_005268607.1.
    XP_005268665.1. XM_005268608.2.
    XP_006719277.1. XM_006719214.1.
    UniGeneiHs.505575.

    Genome annotation databases

    EnsembliENST00000356317; ENSP00000348668; ENSG00000139629.
    ENST00000543196; ENSP00000444171; ENSG00000139629.
    GeneIDi11226.
    KEGGihsa:11226.
    UCSCiuc001ryk.2. human.

    Polymorphism databases

    DMDMi51316028.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Polypeptide N-acetylgalactosaminyltransferase 6

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08565 mRNA. Translation: CAA69876.1 .
    AK025961 mRNA. Translation: BAB15297.1 . Different initiation.
    AK074658 mRNA. Translation: BAC11118.1 .
    BC035822 mRNA. Translation: AAH35822.2 .
    CCDSi CCDS8813.1.
    RefSeqi NP_009141.2. NM_007210.3.
    XP_005268664.1. XM_005268607.1.
    XP_005268665.1. XM_005268608.2.
    XP_006719277.1. XM_006719214.1.
    UniGenei Hs.505575.

    3D structure databases

    ProteinModelPortali Q8NCL4.
    SMRi Q8NCL4. Positions 142-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116393. 4 interactions.
    IntActi Q8NCL4. 4 interactions.
    MINTi MINT-5006466.
    STRINGi 9606.ENSP00000348668.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    PTM databases

    PhosphoSitei Q8NCL4.

    Polymorphism databases

    DMDMi 51316028.

    Proteomic databases

    MaxQBi Q8NCL4.
    PaxDbi Q8NCL4.
    PRIDEi Q8NCL4.

    Protocols and materials databases

    DNASUi 11226.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000356317 ; ENSP00000348668 ; ENSG00000139629 .
    ENST00000543196 ; ENSP00000444171 ; ENSG00000139629 .
    GeneIDi 11226.
    KEGGi hsa:11226.
    UCSCi uc001ryk.2. human.

    Organism-specific databases

    CTDi 11226.
    GeneCardsi GC12M051769.
    HGNCi HGNC:4128. GALNT6.
    HPAi HPA011762.
    HPA017086.
    MIMi 605148. gene.
    neXtProti NX_Q8NCL4.
    PharmGKBi PA28541.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG239675.
    HOGENOMi HOG000038227.
    HOVERGENi HBG051699.
    InParanoidi Q8NCL4.
    KOi K00710.
    OMAi NQCLDVG.
    OrthoDBi EOG7J9VP2.
    PhylomeDBi Q8NCL4.
    TreeFami TF313267.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.1.41. 2681.
    Reactomei REACT_115606. O-linked glycosylation of mucins.

    Miscellaneous databases

    ChiTaRSi GALNT6. human.
    GeneWikii GALNT6.
    GenomeRNAii 11226.
    NextBioi 42726.
    PROi Q8NCL4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8NCL4.
    Bgeei Q8NCL4.
    CleanExi HS_GALNT6.
    Genevestigatori Q8NCL4.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a close homologue of human UDP-N-acetyl--D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy."
      Bennett E.P., Hassan H., Mandel U., Hollingsworth M.A., Akisawa N., Ikematsu Y., Merkx G., Geurts van Kessel A., Olofsson S., Clausen H.
      J. Biol. Chem. 274:25362-25370(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Gastric carcinoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney epithelium and Mammary gland.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lymph.

    Entry informationi

    Entry nameiGALT6_HUMAN
    AccessioniPrimary (citable) accession number: Q8NCL4
    Secondary accession number(s): Q8IYH4, Q9H6G2, Q9UIV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3