Reviewed, UniProtKB/Swiss-Prot Q8NCL4 (GALT6_HUMAN)

Names and origin

Protein names

Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 6
    EC=2.4.1.41
Alternative name(s):
    Polypeptide GalNAc transferase 6
      Short name=pp-GaNTase 6
      Short name=GalNAc-T6
    Protein-UDP acetylgalactosaminyltransferase 6
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6

Gene names

Name:

GALNT6

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.
Catalytic activity	UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide. Ref.1
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in placenta and trachea. Weakly expressed in brain and pancreas. Expressed in fibroblast. Weakly or not expressed in lung, liver, muscle, kidney, spleen, thymus, prostate, testis, ovary, intestine, colon, leukocyte, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland and bone marrow. Ref.1
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid O-linked glycosylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activity Ref.1 Traceable author statement. Source: ProtInc sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Polypeptide N-acetylgalactosaminyltransferase 6		PRO_0000059114
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 28	20	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 622	594	Lumenal Potential
	Domain	496 – 622	127	Ricin B-type lectin
	Region	176 – 285	110	Catalytic subdomain A
	Region	348 – 410	63	Catalytic subdomain B
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	476	1	N-linked (GlcNAc...) Potential
	Disulfide bond	509 ↔ 527		By similarity
	Disulfide bond	553 ↔ 566		By similarity
	Disulfide bond	597 ↔ 610		By similarity
Natural variations
	Natural variant	423	1	V → I: dbSNP rs747300.		VAR_019580
Experimental info
	Sequence conflict	362	1	S → P in CAA69876. Ref.1
	Sequence conflict	454	1	A → T in BAC11118. Ref.2
	Sequence conflict	551	1	Q → R in BAC11118. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8NCL4-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 38E63FF2AFB486EF	FASTA	622	71,159
	10 20 30 40 50 60 MRLLRRRHMP LRLAMVGCAF VLFLFLLHRD VSSREEATEK PWLKSLVSRK DHVLDLMLEA 70 80 90 100 110 120 MNNLRDSMPK LQIRAPEAQQ TLFSINQSCL PGFYTPAELK PFWERPPQDP NAPGADGKAF 130 140 150 160 170 180 QKSKWTPLET QEKEEGYKKH CFNAFASDRI SLQRSLGPDT RPPECVDQKF RRCPPLATTS 190 200 210 220 230 240 VIIVFHNEAW STLLRTVYSV LHTTPAILLK EIILVDDAST EEHLKEKLEQ YVKQLQVVRV 250 260 270 280 290 300 VRQEERKGLI TARLLGASVA QAEVLTFLDA HCECFHGWLE PLLARIAEDK TVVVSPDIVT 310 320 330 340 350 360 IDLNTFEFAK PVQRGRVHSR GNFDWSLTFG WETLPPHEKQ RRKDETYPIK SPTFAGGLFS 370 380 390 400 410 420 ISKSYFEHIG TYDNQMEIWG GENVEMSFRV WQCGGQLEII PCSVVGHVFR TKSPHTFPKG 430 440 450 460 470 480 TSVIARNQVR LAEVWMDSYK KIFYRRNLQA AKMAQEKSFG DISERLQLRE QLHCHNFSWY 490 500 510 520 530 540 LHNVYPEMFV PDLTPTFYGA IKNLGTNQCL DVGENNRGGK PLIMYSCHGL GGNQYFEYTT 550 560 570 580 590 600 QRDLRHNIAK QLCLHVSKGA LGLGSCHFTG KNSQVPKDEE WELAQDQLIR NSGSGTCLTS 610 620 QDKKPAMAPC NPSDPHQLWL FV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a close homologue of human UDP-N-acetyl--D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy." Bennett E.P., Hassan H., Mandel U., Hollingsworth M.A., Akisawa N., Ikematsu Y., Merkx G., Geurts van Kessel A., Olofsson S., Clausen H. J. Biol. Chem. 274:25362-25370(1999) [PubMed: 10464263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY. Tissue: Gastric carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney epithelium and Mammary gland.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase 6

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08565 mRNA. Translation: CAA69876.1. AK025961 mRNA. Translation: BAB15297.1. Different initiation. AK074658 mRNA. Translation: BAC11118.1. BC035822 mRNA. Translation: AAH35822.2.
IPI	IPI00026991.
RefSeq	NP_009141.2.
UniGene	Hs.505575
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NCL4.
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Proteomic databases
PRIDE	Q8NCL4.
Genome annotation databases
Ensembl	ENST00000356317; ENSP00000348668; ENSG00000139629; Homo sapiens. [Genome view]
GeneID	11226.
KEGG	hsa:11226.
UCSC	uc001ryk.1. human.
Organism-specific databases
CTD	11226.
GeneCards	GC12M050033.
H-InvDB	HIX0010638.
HGNC	HGNC:4128. GALNT6.
HPA	HPA011762. HPA017086.
MIM	605148. gene.
PharmGKB	PA28541.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8NCL4.
HOVERGEN	Q8NCL4.
OMA	QDQLIRN.
Enzyme and pathway databases
BRENDA	2.4.1.41. 247.
Gene expression databases
ArrayExpress	Q8NCL4.
Bgee	Q8NCL4.
CleanEx	HS_GALNT6.
Genevestigator	Q8NCL4.
GermOnline	ENSG00000139629. Homo sapiens.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	42726.
SOURCE	Search...

Entry information

Entry name

GALT6_HUMAN

Accession

Primary (citable) accession number: Q8NCL4
Secondary accession number(s): Q8IYH4, Q9H6G2, Q9UIV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.